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1a4r

G12V MUTANT OF HUMAN PLACENTAL CDC42 GTPASE IN THE GDP FORM

Method: X-RAY DIFFRACTION D_max: 74.9 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1a4r
deposition_date	1998-02-02
title	G12V MUTANT OF HUMAN PLACENTAL CDC42 GTPASE IN THE GDP FORM
keywords	HYDROLASE, GTPASE, SIGNAL TRANSDUCTION; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	23.43 Å
rg_electron	22.49 Å
i0	30546100.00
molecular_weight	43249.0 kDa
excluded_volume	54565 Å³
envelope_volume	65226 Å³
shell_volume	24180 Å³
envelope_diameter	76.5 Å
shell_rg	29.22 Å
envelope_rg	22.70 Å
shape_rg	22.50 Å
total_rg	23.34 Å
total_atoms	3033
n_residues	380
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	74.9 Å
rg_real	23.37 Å
rg_real_error	0.43 Å
i0_real	3.0550e+07
i0_real_error	4.0880e+05
rg_reciprocal	23.39 Å
i0_reciprocal	30550000.0000
total_estimate	0.9034
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	27.7 Å
skewness	0.262
kurtosis	-0.439
angular_range	— – 0.3400 Å⁻¹
current_alpha	0.0000
highest_alpha	5084000.0000
n_real_points	66
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.917; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.991

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	G25K GTP-BINDING PROTEIN
formula_weight	21325.7 kDa
mutation	YES
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	3087

MQTIKCVVVGDVAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	GUANOSINE-5'-DIPHOSPHATE
formula_weight	443.2 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	AMINOPHOSPHONIC ACID-GUANYLATE ESTER
formula_weight	442.2 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	54

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6. Citations (1)

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length_a	98.650 Å
length_b	98.650 Å
length_c	104.160 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 41 21 2
int_tables_number	92
crystal_system	—

resolution	2.500 Å
resolution_low	30.000 Å
r_factor	0.2280
r_work	0.2280
r_free	0.2800

fit	/app/data/saxs_computation/a4/1a4r/1a4r.fit
pddf	/app/data/pr_computation/a4/1a4r/1a4r.pr.out
profile	/app/data/saxs_computation/a4/1a4r/1a4r.dat