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1ad2

RIBOSOMAL PROTEIN L1 MUTANT WITH SERINE 179 REPLACED BY CYSTEINE

Method: X-RAY DIFFRACTION D_max: 62.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ad2
deposition_date	1997-02-20
title	RIBOSOMAL PROTEIN L1 MUTANT WITH SERINE 179 REPLACED BY CYSTEINE
keywords	RIBOSOMAL PROTEIN, RNA BINDING, PROTEIN SYNTHESIS, MUTANT; RIBOSOMAL PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	19.71 Å
rg_electron	18.80 Å
i0	11570400.00
molecular_weight	24903.0 kDa
excluded_volume	31042 Å³
envelope_volume	38122 Å³
shell_volume	17374 Å³
envelope_diameter	64.3 Å
shell_rg	24.48 Å
envelope_rg	18.91 Å
shape_rg	18.83 Å
total_rg	19.58 Å
total_atoms	1736
n_residues	224
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	62.6 Å
rg_real	19.62 Å
rg_real_error	0.40 Å
i0_real	1.1570e+07
i0_real_error	1.5040e+05
rg_reciprocal	19.64 Å
i0_reciprocal	11570000.0000
total_estimate	0.8229
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	23.8 Å
skewness	0.209
kurtosis	-0.448
angular_range	— – 0.4050 Å⁻¹
current_alpha	0.0000
highest_alpha	2347000.0000
n_real_points	72
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.899; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	RIBOSOMAL PROTEIN L1
formula_weight	24752.6 kDa
mutation	S179C
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1858

PKHGKRYRALLEKVDPNKIYTIDEAAHLVKELATAKFDETVEVHAKLGIDPRRSDQNVRGTVSLPHGLGKQVRVLAIAKGEKIKEAEEAGADYVGGEEIIQKILDGWMDFDAVVATPDVMGAVGSKLGRILGPRGLLPNPKAGTVGFNIGEIIREIKAGRIEFRNDKTGAIHAPVGKACFPPEKLADNIRAFIRALEAHKPEGAKGTFLRSVYVTTTMGPSVRINPHS

Source

organism	Thermus thermophilus
ncbi_taxonomy_id	274
host_organism	Escherichia coli BL21

Entity 2 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	3

Entity 3 non-polymer

description	MERCURY (II) ION
formula_weight	200.6 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	(4R)-2-METHYLPENTANE-2,4-DIOL
formula_weight	118.2 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	122

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6. Citations (1)

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length_a	76.100 Å
length_b	61.400 Å
length_c	46.100 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	1.900 Å
resolution_low	10.000 Å
r_factor	0.2030
r_work	0.2030
r_free	0.2700

fit	/app/data/saxs_computation/ad/1ad2/1ad2.fit
pddf	/app/data/pr_computation/ad/1ad2/1ad2.pr.out
profile	/app/data/saxs_computation/ad/1ad2/1ad2.dat