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1apl

CRYSTAL STRUCTURE OF A MAT-ALPHA2 HOMEODOMAIN-OPERATOR COMPLEX SUGGESTS A GENERAL MODEL FOR HOMEODOMAIN-DNA INTERACTIONS

Method: X-RAY DIFFRACTION D_max: 80.9 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1apl
deposition_date	1993-10-04
title	CRYSTAL STRUCTURE OF A MAT-ALPHA2 HOMEODOMAIN-OPERATOR COMPLEX SUGGESTS A GENERAL MODEL FOR HOMEODOMAIN-DNA INTERACTIONS
keywords	PROTEIN-DNA COMPLEX, DOUBLE HELIX, DNA BINDING PROTEIN-DNA COMPLEX; DNA BINDING PROTEIN/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	23.19 Å
rg_electron	23.25 Å
i0	20291000.00
molecular_weight	26148.0 kDa
excluded_volume	29006 Å³
envelope_volume	39672 Å³
shell_volume	15435 Å³
envelope_diameter	76.3 Å
shell_rg	28.27 Å
envelope_rg	22.94 Å
shape_rg	23.24 Å
total_rg	23.77 Å
total_atoms	1791
n_residues	159
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	80.9 Å
rg_real	23.37 Å
rg_real_error	0.83 Å
i0_real	2.0290e+07
i0_real_error	3.0030e+05
rg_reciprocal	23.33 Å
i0_reciprocal	20290000.0000
total_estimate	0.7122
solution_quality	REASONABLE a REASONABLE solution
n_peaks	3
r_peak_primary	19.9 Å
skewness	0.416
kurtosis	-0.691
angular_range	— – 0.3400 Å⁻¹
current_alpha	0.0000
highest_alpha	1800000.0000
n_real_points	66
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.579; Stabil: 0.990; Sysdev: 1.000; Positv: 1.000; Valcen: 0.547; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	;DNA (5'-D(APCPAPTPGPTPAPAPTPTPCPAPTPTPTPAPC PAPCPGP*C)-3') ;
formula_weight	6381.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	A
atom_count	1791

ACATGTAATTCATTTACACGC

Entity 2 polymer

description	;DNA (5'-D(TPGPCPGPTPGPTPAPAPAPTPGPAPAPTPTPA PCPAPTP*G)-3') ;
formula_weight	6501.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	B

TGCGTGTAAATGAATTACATG

Entity 3 polymer

description	PROTEIN (MAT-ALPHA2 HOMEODOMAIN)
formula_weight	9773.3 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,D

TKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE

Source

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932
host_organism	Escherichia coli

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6. Citations (2)

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length_a	60.780 Å
length_b	39.930 Å
length_c	68.790 Å
angle_alpha	90.00 °
angle_beta	97.35 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.700 Å
resolution_low	8.000 Å
r_factor	0.2260
r_work	0.2260
r_free	—

fit	/app/data/saxs_computation/ap/1apl/1apl.fit
pddf	/app/data/pr_computation/ap/1apl/1apl.pr.out
profile	/app/data/saxs_computation/ap/1apl/1apl.dat

CRYSTAL STRUCTURE OF A MAT-ALPHA2 HOMEODOMAIN-OPERATOR COMPLEX SUGGESTS A GENERAL MODEL FOR HOMEODOMAIN-DNA INTERACTIONS

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)