MELITTIN
Apis mellifera
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 44–69 | Mutation：PRO 14 REPLACED WITH D-PRO Non-standard monomer：Yes (specific site not provided by mmCIF) | No other associated polymer | SOLUTION NMR NMR measurement conditions：pH 5.2；277 K；Ionic strength (raw mmCIF value) 2mM；Pressure 1 NMR sample composition：METHANOL | Resolution not provided |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 1BH1 | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2MLT MELITTIN Deposited 1990-10-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
44–69（26 aa）
Chain B
44–69（26 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 2MW6 Structure of the bee venom toxin melittin with [(C5H5)Ru]+ fragment attached to the tryptophan residue Deposited 2014-10-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
44–69（26 aa）
Fragment：residues 44-69
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3UQ [(1,2,3,4,5-eta)-cyclopentadienyl][(1,2,3,4,4a,8a-eta)-naphthalene]ruthenium(1+) × 1 |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Pressure 1
NMR sample composition
6 mM Ru-melittin-1, CD3OH | CD3OH
|
Resolution not provided |
| 3QRX Chlamydomonas reinhardtii centrin bound to melittin Deposited 2011-02-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
44–69（26 aa）
Fragment：residues 44-69
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 L of the Crcen-MLT (1:1.5 molar ratio, 10mg/mL total) solution with 2 L of a precipitant solution containing 50 mM HEPES, pH 7.5, 0.2 M KCl, and 40% v/v pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.341 |
| 3QRX Chlamydomonas reinhardtii centrin bound to melittin Deposited 2011-02-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
44–69（26 aa）
Fragment：residues 44-69
|
Not recorded | CA CALCIUM ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 L of the Crcen-MLT (1:1.5 molar ratio, 10mg/mL total) solution with 2 L of a precipitant solution containing 50 mM HEPES, pH 7.5, 0.2 M KCl, and 40% v/v pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.341 |
| 6DST Recombinant melittin Deposited 2018-06-14 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
44–69（26 aa）
Fragment：residues 44-69
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR measurement conditions
pH 7；285 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.05 mM [U-95% 13C; U-95% 15N] melittin, 10 % v/v [U-2H] glycerol, 10 mM potassium phosphate buffer, trifluoroethanol/water | trifluoroethanol/water
NMR sample composition
0.050 mM [U-95% 13C; U-95% 15N] Melittin, 10 mM potassium phosphate buffer, trifluoroethanol/water | trifluoroethanol/water
|
Resolution not provided |
| 6O4M Racemic melittin Deposited 2019-02-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain C
44–69（26 aa）
Fragment：residues 44-69
Chain D
44–69（26 aa）
Fragment：residues 44-69
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.05 M ammonium sulfate, 0.1 M BIS-TRIS pH = 6.5, 30% v/v pentaerythritol ethoxylate (15/4 EO/OH)
|
Resolution 1.27 Å R-free 0.235 |
| 6O4M Racemic melittin Deposited 2019-02-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
44–69（26 aa）
Fragment：residues 44-69
Chain B
44–69（26 aa）
Fragment：residues 44-69
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.05 M ammonium sulfate, 0.1 M BIS-TRIS pH = 6.5, 30% v/v pentaerythritol ethoxylate (15/4 EO/OH)
|
Resolution 1.27 Å R-free 0.235 |
| 8AHS Crystal structure of human Ca2+/Calmodulin in complex with melittin Deposited 2022-07-22 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
44–69（26 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；0.2M NaCl, 22w/v% PEG3350, 0.1M Bis-Tris, pH 5.4
|
Resolution 2.48 Å R-free 0.270 |
| 8AHT Crystal structure of Plasmodium falciparum Ca2+/Calmodulin in complex with melittin Deposited 2022-07-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: dimeric |
Chain F
44–69（26 aa）
Chain G
44–69（26 aa）
Chain H
44–69（26 aa）
Chain I
44–69（26 aa）
|
Not recorded | CA CALCIUM ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；0.25M NaCl, 30w/v% PEG3350, 0.1M Bis-Tris buffer, pH 6.0
|
Resolution 2.20 Å R-free 0.266 |
7 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | MEL_APIME |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–26； UniProt 44–69 |
