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1c4z

STRUCTURE OF AN E6AP-UBCH7 COMPLEX: INSIGHTS INTO THE UBIQUITINATION PATHWAY

Method: X-RAY DIFFRACTION D_max: 114.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1c4z
deposition_date	1999-10-14
title	STRUCTURE OF AN E6AP-UBCH7 COMPLEX: INSIGHTS INTO THE UBIQUITINATION PATHWAY
keywords	BILOBAL STRUCTURE, ELONGATED SHAPE, E3 UBIQUITIN LIGASE, E2 UBIQUITIN CONJUGATING ENZYME, LIGASE; LIGASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.89 Å
rg_electron	35.04 Å
i0	274039000.00
molecular_weight	137280.0 kDa
excluded_volume	173420 Å³
envelope_volume	228930 Å³
shell_volume	54261 Å³
envelope_diameter	126.2 Å
shell_rg	41.57 Å
envelope_rg	34.89 Å
shape_rg	35.03 Å
total_rg	35.58 Å
total_atoms	9681
n_residues	1194
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	114.4 Å
rg_real	35.80 Å
rg_real_error	0.86 Å
i0_real	2.7400e+08
i0_real_error	4.9540e+06
rg_reciprocal	35.86 Å
i0_reciprocal	274100000.0000
total_estimate	0.8838
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	44.2 Å
skewness	0.312
kurtosis	-0.275
angular_range	— – 0.2200 Å⁻¹
current_alpha	0.0000
highest_alpha	46420000.0000
n_real_points	45
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.887; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.828

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	UBIQUITIN-PROTEIN LIGASE E3A
formula_weight	41540.4 kDa
ec_number	6.3.2.-
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,B,C
atom_count	10040

QLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 polymer

description	UBIQUITIN CONJUGATING ENZYME E2
formula_weight	17889.6 kDa
ec_number	6.3.2.19
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	359

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6. Citations (1)

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length_a	103.400 Å
length_b	112.700 Å
length_c	123.800 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	12

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.600 Å
resolution_low	15.000 Å
r_factor	0.2340
r_work	0.2370
r_free	0.2900

fit	/app/data/saxs_computation/c4/1c4z/1c4z.fit
pddf	/app/data/pr_computation/c4/1c4z/1c4z.pr.out
profile	/app/data/saxs_computation/c4/1c4z/1c4z.dat

STRUCTURE OF AN E6AP-UBCH7 COMPLEX: INSIGHTS INTO THE UBIQUITINATION PATHWAY

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)