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1d8t

CRYSTAL STRUCTURE OF ELONGATION FACTOR, TU (EF-TU-MGGDP) COMPLEXED WITH GE2270A, A THIAZOLYL PEPTIDE ANTIBIOTIC

Method: X-RAY DIFFRACTION D_max: 122.3 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1d8t
deposition_date	1999-10-25
title	CRYSTAL STRUCTURE OF ELONGATION FACTOR, TU (EF-TU-MGGDP) COMPLEXED WITH GE2270A, A THIAZOLYL PEPTIDE ANTIBIOTIC
keywords	;HYDROLASE-ANTIBIOTIC COMPLEX, THIOPEPTIDE, ANTIBIOTIC, ANTIBACTERIAL, THIAZOLE, OXAZOLE, HYDROLASE, GTPASE, TRANSLATION ELONGATION FACTOR, PROTEIN SYNTHESIS, NUCLEOTIDE-BINDING, PHOSPHORYLATION ;; HYDROLASE/ANTIBIOTIC
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.17 Å
rg_electron	35.07 Å
i0	131133000.00
molecular_weight	90541.0 kDa
excluded_volume	112830 Å³
envelope_volume	145830 Å³
shell_volume	36630 Å³
envelope_diameter	128.0 Å
shell_rg	39.02 Å
envelope_rg	34.73 Å
shape_rg	35.03 Å
total_rg	35.48 Å
total_atoms	6332
n_residues	784
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	122.3 Å
rg_real	35.39 Å
rg_real_error	1.13 Å
i0_real	1.3110e+08
i0_real_error	2.0540e+06
rg_reciprocal	35.26 Å
i0_reciprocal	131100000.0000
total_estimate	0.6331
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	38.0 Å
skewness	0.497
kurtosis	-0.188
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	13440000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.819; Stabil: 1.000; Sysdev: 0.062; Positv: 1.000; Valcen: 0.863; Smooth: 0.720

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	ELONGATION FACTOR TU
formula_weight	43209.3 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	6718

SKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG

Entity 2 polymer

description	THIOCILLIN GE2270
formula_weight	1484.7 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,D

SCNCVCGFCCSCSPX

Entity 3 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	GUANOSINE-5'-DIPHOSPHATE
formula_weight	443.2 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	ACETATE ION
formula_weight	59.0 kDa
src_method	syn
entity_count	30

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	379

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6. Citations (1)

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length_a	133.470 Å
length_b	45.170 Å
length_c	144.000 Å
angle_alpha	90.00 °
angle_beta	94.64 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.350 Å
resolution_low	24.440 Å
r_factor	0.2000
r_work	0.2000
r_free	0.2460

fit	/app/data/saxs_computation/d8/1d8t/1d8t.fit
pddf	/app/data/pr_computation/d8/1d8t/1d8t.pr.out
profile	/app/data/saxs_computation/d8/1d8t/1d8t.dat

CRYSTAL STRUCTURE OF ELONGATION FACTOR, TU (EF-TU-MGGDP) COMPLEXED WITH GE2270A, A THIAZOLYL PEPTIDE ANTIBIOTIC

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)