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1e0j

gp4d helicase from phage T7 ADPNP complex

Method: X-RAY DIFFRACTION D_max: 135.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e0j
deposition_date	2000-03-30
title	gp4d helicase from phage T7 ADPNP complex
keywords	HELICASE, ATPASE, DNA REPLICATION; HELICASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	42.27 Å
rg_electron	41.80 Å
i0	583682000.00
molecular_weight	191410.0 kDa
excluded_volume	237160 Å³
envelope_volume	331150 Å³
shell_volume	65241 Å³
envelope_diameter	134.3 Å
shell_rg	47.60 Å
envelope_rg	40.95 Å
shape_rg	41.84 Å
total_rg	41.94 Å
total_atoms	13392
n_residues	1726
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	135.1 Å
rg_real	42.14 Å
rg_real_error	0.83 Å
i0_real	5.8370e+08
i0_real_error	9.3980e+06
rg_reciprocal	42.27 Å
i0_reciprocal	583800000.0000
total_estimate	0.8925
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	58.4 Å
skewness	0.140
kurtosis	-0.567
angular_range	— – 0.1850 Å⁻¹
current_alpha	0.0001
highest_alpha	115700000.0000
n_real_points	38
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.923; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.830

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	DNA HELICASE
formula_weight	31755.1 kDa
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F
atom_count	13392

IPDGVVSALSLRERIREHLSSEESVGLLFSGCTGINDKTLGARGGEVIMVTSGSGMGKSTFVRQQALQWGTAMGKKVGLAMLEESVEETAEDLIGLHNRVRLRQSDSLKREIIENGKFDQWFDELFGNDTFHLYDSFAEAETDRLLAKLAYMRSGLGCDVIILDHISIVVSASGESDERKMIDNLMTKLKGFAKSTGVVLVVICHLKNPDKGKAHEEGRPVSITDLRGSGALRQLSDTIIALERNQQGDMPNLVLVRILKCRFTGDTGIAGYMEYNKETGWLEPSSYSG

Source

organism	PHAGE T7
ncbi_taxonomy_id	10760
host_organism	ESCHERICHIA COLI

Entity 2 non-polymer

description	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
formula_weight	506.2 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	4

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6. Citations (1)

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length_a	119.180 Å
length_b	119.180 Å
length_c	283.420 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	48

space_group	P 41 21 2
int_tables_number	92
crystal_system	—

resolution	3.000 Å
resolution_low	20.000 Å
r_factor	0.2530
r_work	0.2530
r_free	0.3040

fit	/app/data/saxs_computation/e0/1e0j/1e0j.fit
pddf	/app/data/pr_computation/e0/1e0j/1e0j.pr.out
profile	/app/data/saxs_computation/e0/1e0j/1e0j.dat

gp4d helicase from phage T7 ADPNP complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction