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1e0v

Xylanase 10A from Sreptomyces lividans. cellobiosyl-enzyme intermediate at 1.7 A

Method: X-RAY DIFFRACTION D_max: 59.3 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e0v
deposition_date	2000-04-10
title	Xylanase 10A from Sreptomyces lividans. cellobiosyl-enzyme intermediate at 1.7 A
keywords	XYLANASE, XYLAN DEGRADATION, HYDROLASE, GLYCOSYL-ENZYME INTERMEDIATE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	19.31 Å
rg_electron	18.15 Å
i0	21024600.00
molecular_weight	33193.0 kDa
excluded_volume	40638 Å³
envelope_volume	44609 Å³
shell_volume	20124 Å³
envelope_diameter	60.2 Å
shell_rg	24.90 Å
envelope_rg	18.31 Å
shape_rg	18.14 Å
total_rg	18.99 Å
total_atoms	2336
n_residues	302
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	59.3 Å
rg_real	19.17 Å
rg_real_error	0.29 Å
i0_real	2.1020e+07
i0_real_error	2.5580e+05
rg_reciprocal	19.19 Å
i0_reciprocal	21020000.0000
total_estimate	0.9023
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	24.2 Å
skewness	0.127
kurtosis	-0.483
angular_range	— – 0.4100 Å⁻¹
current_alpha	0.0000
highest_alpha	4967000.0000
n_real_points	72
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.913; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.989; Smooth: 0.997

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	ENDO-1,4-BETA-XYLANASE A
formula_weight	34085.4 kDa
ec_number	3.2.1.8
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2887

AESTLGAAAAQSGRYFGTAIASGRLSDSTYTSIAGREFNMVTAENEMKIDATEPQRGQFNFSSADRVYNWAVQNGKQVRGHTLAWHSQQPGWMQSLSGSALRQAMIDHINGVMAHYKGKIVQWDVVNEAFADGSSGARRDSNLQRSGNDWIEVAFRTARAADPSAKLCYNDYNVENWTWAKTQAMYNMVRDFKQRGVPIDCVGFQSHFNSGSPYNSNFRTTLQNFAALGVDVAITELDIQGAPASTYANVTNDCLAVSRCLGITVWGVRDSDSWRSEQTPLLFNNDGSKKAAYTAVLDALNGGASSEPPADGG

Source

organism	STREPTOMYCES LIVIDANS
ncbi_taxonomy_id	1916
host_organism	STREPTOMYCES LIVIDANS

Entity 2 branched

description	beta-D-glucopyranose-(1-4)-2-deoxy-2-fluoro-alpha-D-glucopyranose
formula_weight	344.3 kDa
src_method	man
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	505

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6. Citations (3)

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length_a	67.870 Å
length_b	46.270 Å
length_c	87.070 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.700 Å
resolution_low	15.000 Å
r_factor	—
r_work	0.1580
r_free	0.1990

fit	/app/data/saxs_computation/e0/1e0v/1e0v.fit
pddf	/app/data/pr_computation/e0/1e0v/1e0v.pr.out
profile	/app/data/saxs_computation/e0/1e0v/1e0v.dat

Xylanase 10A from Sreptomyces lividans. cellobiosyl-enzyme intermediate at 1.7 A

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction