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1e0x

XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A

Method: X-RAY DIFFRACTION D_max: 93.7 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e0x
deposition_date	2000-04-10
title	XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A
keywords	GLYCOSIDE HYDROLASE FAMILY 10, HYDROLASE, XYLAN DEGRADATION, GLYCOSYL-ENZYME INTERMEDIATE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.76 Å
rg_electron	26.95 Å
i0	85193600.00
molecular_weight	68395.0 kDa
excluded_volume	83678 Å³
envelope_volume	98625 Å³
shell_volume	30669 Å³
envelope_diameter	97.4 Å
shell_rg	34.11 Å
envelope_rg	26.88 Å
shape_rg	26.94 Å
total_rg	27.61 Å
total_atoms	4814
n_residues	620
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	93.7 Å
rg_real	28.72 Å
rg_real_error	0.27 Å
i0_real	8.4830e+07
i0_real_error	1.1170e+06
rg_reciprocal	27.77 Å
i0_reciprocal	85190000.0000
total_estimate	0.6770
solution_quality	REASONABLE a REASONABLE solution
n_peaks	1
r_peak_primary	29.6 Å
skewness	0.438
kurtosis	-0.303
angular_range	— – 0.2850 Å⁻¹
current_alpha	4.3680
highest_alpha	32550000.0000
n_real_points	58
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.876; Stabil: 0.900; Sysdev: 0.000; Positv: 1.000; Valcen: 0.957; Smooth: 0.558

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	ENDO-1,4-BETA-XYLANASE A
formula_weight	34129.5 kDa
ec_number	3.2.1.8
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	5594

AESTLGAAAAQSGRYFGTAIASGRLSDSTYTSIAGREFNMVTAENEMKIDATEPQRGQFNFSSADRVYNWAVQNGKQVRGHTLAWHSQQPGWMQSLSGSALRQAMIDHINGVMAHYKGKIVQWDVVNEAFADGSSGARRDSNLQRSGNDWIEVAFRTARAADPSAKLCYNDYNVENWTWAKTQAMYNMVRDFKQRGVPIDCVGFQSHFNSGSPYNSNFRTTLQNFAALGVDVAITELDIQGAPASTYANVTNDCLAVSRCLGITVWGVRDSDSWRSEQTPLLFNNDGSKKAAYTAVLDALNGGDSSEPPADGG

Source

organism	STREPTOMYCES LIVIDANS
ncbi_taxonomy_id	1916
host_organism	STREPTOMYCES LIVIDANS

Entity 2 branched

description	beta-D-xylopyranose-(1-4)-2-deoxy-2-fluoro-alpha-D-xylopyranose
formula_weight	284.2 kDa
src_method	man
entity_count	2

Entity 3 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	3

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	717

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6. Citations (2)

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length_a	49.210 Å
length_b	81.060 Å
length_c	72.810 Å
angle_alpha	90.00 °
angle_beta	102.79 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.650 Å
resolution_low	15.000 Å
r_factor	—
r_work	0.1260
r_free	0.1620

fit	/app/data/saxs_computation/e0/1e0x/1e0x.fit
pddf	/app/data/pr_computation/e0/1e0x/1e0x.pr.out
profile	/app/data/saxs_computation/e0/1e0x/1e0x.dat

XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction