STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE

Method: X-RAY DIFFRACTION D_max: 65.6 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1edz
deposition_date	2000-01-28
title	STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE
keywords	nucleotide-binding domain, monofunctional, dehydrogenase, folate, oxidoreductase; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	20.99 Å
rg_electron	19.81 Å
i0	21411000.00
molecular_weight	35698.0 kDa
excluded_volume	44950 Å³
envelope_volume	52356 Å³
shell_volume	21895 Å³
envelope_diameter	67.0 Å
shell_rg	26.40 Å
envelope_rg	19.94 Å
shape_rg	19.82 Å
total_rg	20.69 Å
total_atoms	2511
n_residues	317
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	65.6 Å
rg_real	20.87 Å
rg_real_error	0.31 Å
i0_real	2.1410e+07
i0_real_error	2.4400e+05
rg_reciprocal	20.89 Å
i0_reciprocal	21410000.0000
total_estimate	0.7403
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	25.7 Å
skewness	0.162
kurtosis	-0.457
angular_range	— – 0.3800 Å⁻¹
current_alpha	0.0000
highest_alpha	3785000.0000
n_real_points	70
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.916; Stabil: 1.000; Sysdev: 0.301; Positv: 1.000; Valcen: 0.992; Smooth: 0.979

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (2)

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length_a	73.980 Å
length_b	73.980 Å
length_c	161.685 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 41 21 2
int_tables_number	92
crystal_system	—

resolution	2.800 Å
resolution_low	20.000 Å
r_factor	0.2650
r_work	0.2600
r_free	0.3510

description	5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE
formula_weight	36284.6 kDa
ec_number	1.5.1.15
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	2533

STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932
host_organism	Escherichia coli

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	22

fit	/app/data/saxs_computation/ed/1edz/1edz.fit
pddf	/app/data/pr_computation/ed/1edz/1edz.pr.out
profile	/app/data/saxs_computation/ed/1edz/1edz.dat