← Back to search

1ej7

CRYSTAL STRUCTURE OF UNACTIVATED TOBACCO RUBISCO WITH BOUND PHOSPHATE IONS

Method: X-RAY DIFFRACTION D_max: 92.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1ej7
deposition_date	2000-03-01
title	CRYSTAL STRUCTURE OF UNACTIVATED TOBACCO RUBISCO WITH BOUND PHOSPHATE IONS
keywords	TIM Barrel, alpha/beta barrel, LYASE; LYASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	26.83 Å
rg_electron	26.40 Å
i0	69999400.00
molecular_weight	65568.0 kDa
excluded_volume	81946 Å³
envelope_volume	99394 Å³
shell_volume	31488 Å³
envelope_diameter	95.1 Å
shell_rg	33.67 Å
envelope_rg	26.99 Å
shape_rg	26.42 Å
total_rg	27.06 Å
total_atoms	4623
n_residues	580
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	92.1 Å
rg_real	26.86 Å
rg_real_error	0.71 Å
i0_real	7.0000e+07
i0_real_error	1.0640e+06
rg_reciprocal	26.85 Å
i0_reciprocal	70000000.0000
total_estimate	0.8691
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	29.7 Å
skewness	0.416
kurtosis	-0.231
angular_range	— – 0.2950 Å⁻¹
current_alpha	0.0000
highest_alpha	20660000.0000
n_real_points	60
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.792; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.935; Smooth: 0.982

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (4)

Entity 1 polymer

description	RUBISCO (LARGE SUBUNIT)
formula_weight	52734.8 kDa
ec_number	4.1.1.39
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	L
atom_count	4790

PQTETKASVGFKAGVKDYKLTYYTPEYQTKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYRIERVVGEKDQYIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPAYVKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEALYKAQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLAHYCRDNGLLLHIHRAMHAVIDRQKNHGIHFRVLAKALRMSGGDHIHSGTVVGKLEGERDITLGFVDLLRDDFVEQDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVKARNEGRDLAQEGNEIIREACKWSPELAAACEVWKEIVFNFAAVDVLDK

Entity 2 polymer

description	RUBISCO (SMALL SUBUNIT)
formula_weight	14573.6 kDa
ec_number	4.1.1.39
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	S

MQVWPPINKKKYETLSYLPDLSQEQLLSEVEYLLKNGWVPCLEFETEHGFVYRENNKSPGYYDGRYWTMWKLPMFGCTDATQVLAEVEEAKKAYPQAWIRIIGFDNVRQVQCISFIAYKPEGY

Entity 3 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	syn
entity_count	3

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	167

▶

6. Citations (1)

▶

length_a	149.180 Å
length_b	149.180 Å
length_c	138.340 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	I 4 2 2
int_tables_number	97
crystal_system	—

resolution	2.450 Å
resolution_low	20.000 Å
r_factor	—
r_work	0.1490
r_free	0.1970

fit	/app/data/saxs_computation/ej/1ej7/1ej7.fit
pddf	/app/data/pr_computation/ej/1ej7/1ej7.pr.out
profile	/app/data/saxs_computation/ej/1ej7/1ej7.dat

CRYSTAL STRUCTURE OF UNACTIVATED TOBACCO RUBISCO WITH BOUND PHOSPHATE IONS

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 non-polymer

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)