1f1z

TNSA, a catalytic component of the TN7 transposition system

Method: X-RAY DIFFRACTION Dmax: 115.0 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1f1z

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1f1z
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1f1z
沉积日期 deposition_date2000-05-21
结构标题 titleTNSA, a catalytic component of the TN7 transposition system
关键词 keywordsrestriction endonuclease fold, DNA BINDING PROTEIN; DNA BINDING PROTEIN
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier30.56
回转半径 Rg (电子) rg_electron30.59
零角强度 I(0) i055962000.00
分子量 molecular_weight59548.0 kDa
排除体积 excluded_volume74893 ų
包络体积 envelope_volume95304 ų
水化壳体积 shell_volume28720 ų
包络直径 envelope_diameter121.2
壳层 Rg shell_rg33.82
包络 Rg envelope_rg30.95
形状 Rg shape_rg30.55
总 Rg total_rg31.03
总原子数 total_atoms4196
残基数 n_residues520
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax115.0
Rg (实空间) rg_real30.96
Rg 误差 (实空间) rg_real_error1.20
I(0) (实空间) i0_real5.5960e+07
I(0) 误差 (实空间) i0_real_error9.1550e+05
Rg (倒空间) rg_reciprocal30.79
I(0) (倒空间) i0_reciprocal55950000.0000
解质量估计 total_estimate0.7617
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary26.6
偏度 Skewness skewness0.668
峰度 Kurtosis kurtosis0.035
角度范围 angular_range— – 0.2600 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha24090000.0000
实空间数据点数 n_real_points53
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.511; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.422; Smooth: 0.943

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (4)

6. 折叠分类 (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd1f1za1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.5 — 'Winged helix' DNA-binding domain
家族 Family familya.4.5.27 — TnsA endonuclease, C-terminal domain
结构域编号 domain_idd1f1za2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.52 — Restriction endonuclease-like
超家族 Superfamily superfamilyc.52.1 — Restriction endonuclease-like
家族 Family familyc.52.1.16 — TnsA endonuclease, N-terminal domain
结构域编号 domain_idd1f1zb1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.5 — 'Winged helix' DNA-binding domain
家族 Family familya.4.5.27 — TnsA endonuclease, C-terminal domain
结构域编号 domain_idd1f1zb2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.52 — Restriction endonuclease-like
超家族 Superfamily superfamilyc.52.1 — Restriction endonuclease-like
家族 Family familyc.52.1.16 — TnsA endonuclease, N-terminal domain

CATH v4.4 (4 domains)

结构域编号 domain_id1f1zA01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology1350 — Trna Endonuclease; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1f1zA02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id1f1zB01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology1350 — Trna Endonuclease; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1f1zB02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

7. 引用文献 (1)

8. 文件与曲线 (10)