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1f5l

UROKINASE PLASMINOGEN ACTIVATOR B-CHAIN-AMILORIDE COMPLEX

Method: X-RAY DIFFRACTION D_max: 60.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1f5l
deposition_date	2000-06-15
title	UROKINASE PLASMINOGEN ACTIVATOR B-CHAIN-AMILORIDE COMPLEX
keywords	UROKINASE, INHIBITOR, SERINE PROTEASE, HUMAN, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	18.31 Å
rg_electron	17.14 Å
i0	14803100.00
molecular_weight	27961.0 kDa
excluded_volume	34541 Å³
envelope_volume	39366 Å³
shell_volume	18698 Å³
envelope_diameter	59.6 Å
shell_rg	24.00 Å
envelope_rg	17.54 Å
shape_rg	17.13 Å
total_rg	18.16 Å
total_atoms	1958
n_residues	245
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	60.5 Å
rg_real	18.20 Å
rg_real_error	0.35 Å
i0_real	1.4800e+07
i0_real_error	1.7500e+05
rg_reciprocal	18.21 Å
i0_reciprocal	14800000.0000
total_estimate	0.8739
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	23.7 Å
skewness	0.171
kurtosis	-0.335
angular_range	— – 0.4350 Å⁻¹
current_alpha	0.0000
highest_alpha	4594000.0000
n_real_points	75
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.796; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.977

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	UROKINASE-TYPE PLASMINOGEN ACTIVATOR
formula_weight	28444.3 kDa
ec_number	3.4.21.73
mutation	C279S
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2006

IIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTISLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	3,5-DIAMINO-N-(AMINOIMINOMETHYL)-6-CHLOROPYRAZINECARBOXAMIDE
formula_weight	229.6 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	48

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6. Citations (1)

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length_a	52.800 Å
length_b	54.600 Å
length_c	82.920 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.100 Å
resolution_low	500.000 Å
r_factor	—
r_work	0.2020
r_free	0.2500

fit	/app/data/saxs_computation/f5/1f5l/1f5l.fit
pddf	/app/data/pr_computation/f5/1f5l/1f5l.pr.out
profile	/app/data/saxs_computation/f5/1f5l/1f5l.dat

UROKINASE PLASMINOGEN ACTIVATOR B-CHAIN-AMILORIDE COMPLEX

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction