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1n9m

Streptavidin Mutant S27A with Biotin at 1.6A Resolution

Method: X-RAY DIFFRACTION Dmax: 68.2 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Streptavidin

Streptomyces avidinii

UniProt P22629

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain A； UniProt 37–163 Chain B； UniProt 37–163 Chain C； UniProt 37–163 Chain D； UniProt 37–163 Fragment：core streptavidin, residues 13-139 Mutation：S27A BTN BIOTIN × 4 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K Resolution 1.60 Å R-free 0.207

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1N9M Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1DF8 S45A MUTANT OF STREPTAVIDIN IN COMPLEX WITH BIOTIN Deposited 1999-11-18 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 3–129（127 aa） Fragment：CORE, RESIDUES 13-139
Chain B 3–129（127 aa） Fragment：CORE, RESIDUES 13-139
Mutation：S45A Mutation：S45A BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；294 K；40% SATURATED AMMONIUM SULFATE, 0.1 M SODIUM ACETATE, pH 4.5, temperature 294.K
Resolution 1.51 Å R-free 0.225
1DF8 S45A MUTANT OF STREPTAVIDIN IN COMPLEX WITH BIOTIN Deposited 1999-11-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 3–129（127 aa） Fragment：CORE, RESIDUES 13-139
Chain B 3–129（127 aa） Fragment：CORE, RESIDUES 13-139
Mutation：S45A Mutation：S45A BTN BIOTIN × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；294 K；40% SATURATED AMMONIUM SULFATE, 0.1 M SODIUM ACETATE, pH 4.5, temperature 294.K
Resolution 1.51 Å R-free 0.225
1HQQ MINIPROTEIN MP-2 (M9A) COMPLEX WITH STREPTAVIDIN Deposited 2000-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–129（129 aa）
Chain B 1–129（129 aa）
Chain C 1–129（129 aa）
Chain D 1–129（129 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；100 mM potassium acetate, ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.249
1HXL MINIPROTEIN MP-2 (V10A) COMPLEX WITH STREPTAVIDIN Deposited 2001-01-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–129（129 aa）
Chain B 1–129（129 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；298 K；100 MM POTASSIUM ACETATE, AMMONIUM SULFATE, pH 4.80, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.80 Å R-free 0.236
1HXZ MINIPROTEIN MP-2 COMPLEX WITH STREPTAVIDIN Deposited 2001-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–129（129 aa）
Chain B 1–129（129 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；100mM potassium acetate, 32% AMMONIUM SULFATE , pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.80 Å R-free 0.230
1HY2 MINIPROTEIN MP-1 COMPLEX WITH STREPTAVIDIN Deposited 2001-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–129（129 aa）
Chain B 1–129（129 aa）
Chain C 1–129（129 aa）
Chain D 1–129（129 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；100mM potassium acetate, ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.258
1I9H CORE STREPTAVIDIN-BNA COMPLEX Deposited 2001-03-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–163（139 aa） Fragment：RESIDUES 25-163
Chain B 25–163（139 aa） Fragment：RESIDUES 25-163
Not recorded BNI 5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID (4-NITRO-PHENYL)-AMIDE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；10% isopropanol, 0.1M NaCitrate, 0.05M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.250
1KFF An engineered streptavidin with improved affinity for the strep-tag II peptide: apo-SAM1 Deposited 2001-11-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–163（126 aa）
Chain B 38–163（126 aa）
Chain C 38–163（126 aa）
Chain D 38–163（126 aa）
Mutation：E44V,S45T,V47R Mutation：E44V,S45T,V47R Mutation：E44V,S45T,V47R Mutation：E44V,S45T,V47R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.3；293 K；100 mM Na2HPO4, 1.2-M (NH4)2SO4, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.229
1KL3 an engineered streptavidin with improved affinity for the strep-tag II peptide : SAm1-StrepII Deposited 2001-12-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 38–163（126 aa）
Chain B 38–163（126 aa）
Chain C 38–163（126 aa）
Chain D 38–163（126 aa）
Mutation：E44V,S45T,V47R Mutation：E44V,S45T,V47R Mutation：E44V,S45T,V47R Mutation：E44V,S45T,V47R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；100 mM Na2HPO4, 1.3-M (NH4)2SO4, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.70 Å R-free 0.217
1KL4 AN ENGINEERED STREPTAVIDIN WITH IMPROVED AFFINITY FOR THE STREP-TAG II PEPTIDE : apo-SAM2 Deposited 2001-12-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–163（126 aa）
Chain B 38–163（126 aa）
Chain C 38–163（126 aa）
Chain D 38–163（126 aa）
Mutation：E44I,S45G,V47R Mutation：E44I,S45G,V47R Mutation：E44I,S45G,V47R Mutation：E44I,S45G,V47R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；50 mM Na3PO4, 50 mM Na3-citrate, 30% PEG 600, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.70 Å R-free 0.228
1KL5 an engineered streptavidin with improved affinity for the strep-tag II peptide : SAm2-StrepII Deposited 2001-12-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 38–163（126 aa）
Chain B 38–163（126 aa）
Chain C 38–163（126 aa）
Chain D 38–163（126 aa）
Mutation：E44I,S45G,V47R Mutation：E44I,S45G,V47R Mutation：E44I,S45G,V47R Mutation：E44I,S45G,V47R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；100 mM Na2HPO4, 1.2-M (NH4)2SO4, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.80 Å R-free 0.221
1LCV streptavidin-norbiotin complex Deposited 2002-04-07 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Not recorded SNR NORBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；1.2 M ammonium sulfate, 0.1M sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.233
1LCV streptavidin-norbiotin complex Deposited 2002-04-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Not recorded SNR NORBIOTIN × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；1.2 M ammonium sulfate, 0.1M sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.233
1LCW streptavidin-homobiotin complex Deposited 2002-04-07 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Not recorded SHM HOMOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；ammonium sulfate, sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.20 Å R-free 0.233
1LCZ streptavidin-BCAP complex Deposited 2002-04-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–159（135 aa）
Chain B 25–159（135 aa）
Not recorded BH7 E-AMINO BIOTINYL CAPROIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；1.2M ammonium sulfate, 0.1M sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.95 Å R-free 0.237
1LCZ streptavidin-BCAP complex Deposited 2002-04-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 25–159（135 aa）
Chain B 25–159（135 aa）
Not recorded BH7 E-AMINO BIOTINYL CAPROIC ACID × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；1.2M ammonium sulfate, 0.1M sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.95 Å R-free 0.237
1MEP Crystal Structure of Streptavidin Double Mutant S45A/D128A with Biotin: Cooperative Hydrogen-Bond Interactions in the Streptavidin-Biotin System. Deposited 2002-08-08 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：Core streptavidin (residues 13-139)
Chain B 37–163（127 aa） Fragment：Core streptavidin (residues 13-139)
Chain C 37–163（127 aa） Fragment：Core streptavidin (residues 13-139)
Chain D 37–163（127 aa） Fragment：Core streptavidin (residues 13-139)
Mutation：S45A, D128A Mutation：S45A, D128A Mutation：S45A, D128A Mutation：S45A, D128A BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；294 K；sodium citrate, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K
Resolution 1.65 Å R-free 0.295
1MK5 Wildtype Core-Streptavidin with Biotin at 1.4A. Deposited 2002-08-28 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：Core Streptavidin (residues 13-139)
Chain B 37–163（127 aa） Fragment：Core Streptavidin (residues 13-139)
Not recorded BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；ammonium sulphate, sodium acetate, sodium chloride, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.40 Å R-free 0.189
1MM9 Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD Deposited 2002-09-03 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：Core Streptavidin (residues 13-139)
Not recorded MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.66 Å R-free 0.195
1MM9 Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD Deposited 2002-09-03 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：Core Streptavidin (residues 13-139)
Not recorded MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.66 Å R-free 0.195
1MM9 Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD Deposited 2002-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 37–163（127 aa） Fragment：Core Streptavidin (residues 13-139)
Not recorded MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.66 Å R-free 0.195
1MOY Streptavidin Mutant with Osteopontin Hexapeptide Insertion Including RGD Deposited 2002-09-10 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Not recorded MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.55 Å R-free 0.185
1MOY Streptavidin Mutant with Osteopontin Hexapeptide Insertion Including RGD Deposited 2002-09-10 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Not recorded MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.55 Å R-free 0.185
1MOY Streptavidin Mutant with Osteopontin Hexapeptide Insertion Including RGD Deposited 2002-09-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Not recorded MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.55 Å R-free 0.185
1N43 Streptavidin Mutant N23A with biotin at 1.89A Deposited 2002-10-30 Different mutation/modification Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain B 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain C 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain D 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Mutation：N23A Mutation：N23A Mutation：N23A Mutation：N23A BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.89 Å R-free 0.301
1N4J STREPTAVIDIN MUTANT N23A AT 2.18A Deposited 2002-10-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Mutation：N23A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.2；293 K；PEG4000, phosphate buffer, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.18 Å R-free 0.253
1N7Y STREPTAVIDIN MUTANT N23E AT 1.96A Deposited 2002-11-18 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain B 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain C 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain D 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Mutation：N23E Mutation：N23E Mutation：N23E Mutation：N23E No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.96 Å R-free 0.284
1N9Y Streptavidin Mutant S27A at 1.5A Resolution Deposited 2002-11-26 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain B 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain C 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain D 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Mutation：S27A Mutation：S27A Mutation：S27A Mutation：S27A MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.53 Å R-free 0.243
1NBX Streptavidin Mutant Y43A at 1.70A Resolution Deposited 2002-12-04 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain B 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain C 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain D 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Mutation：Y43A Mutation：Y43A Mutation：Y43A Mutation：Y43A MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.273
1NC9 STREPTAVIDIN MUTANT Y43A WITH IMINOBIOTIN AT 1.8A RESOLUTION Deposited 2002-12-05 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core strepavidin, residues 13-139
Chain B 37–163（127 aa） Fragment：core strepavidin, residues 13-139
Chain C 37–163（127 aa） Fragment：core strepavidin, residues 13-139
Chain D 37–163（127 aa） Fragment：core strepavidin, residues 13-139
Mutation：Y43A Mutation：Y43A Mutation：Y43A Mutation：Y43A IMI 2-IMINOBIOTIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.266
1NDJ Streptavidin Mutant Y43F with Biotin at 1.81A Resolution Deposited 2002-12-09 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain B 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain C 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Chain D 37–163（127 aa） Fragment：core streptavidin, residues 13-139
Mutation：Y43F Mutation：Y43F Mutation：Y43F Mutation：Y43F BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.81 Å R-free 0.312
1NQM Structure of Savm-W120K, streptavidin mutant Deposited 2003-01-22 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 24–159（136 aa）
Chain B 24–159（136 aa）
Chain C 24–159（136 aa）
Chain D 24–159（136 aa）
Mutation：W120K Mutation：W120K Mutation：W120K Mutation：W120K BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.45；293 K；25% PEG 6K, 0.3M sodium acetate, 0.1M cac. buffer, pH 6.45, VAPOR DIFFUSION, HANGING DROP, temperature 20K
Resolution 1.70 Å R-free 0.240
1PTS CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN Deposited 1992-07-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1PTS CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN Deposited 1992-07-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1RST COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE Deposited 1995-10-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 38–163（126 aa） Fragment：RESIDUES 13 - 139
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.70 Å R-free 0.227
1RSU COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG II PEPTIDE Deposited 1995-10-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 38–163（126 aa） Fragment：RESIDUES 13 - 139
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.70 Å R-free 0.213
1RXH Crystal structure of streptavidin mutant L124R (M1) complexed with biotinyl p-nitroanilide (BNI) Deposited 2003-12-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Mutation：L124R, L324R Mutation：L124R, L324R BNI 5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID (4-NITRO-PHENYL)-AMIDE × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.90 Å R-free 0.309
1RXJ Crystal structure of streptavidin mutant (M2) where the L3,4 loop was replace by that of avidin Deposited 2003-12-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Chain C 39–159（121 aa）
Chain D 39–159（121 aa）
Not recorded BNI 5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID (4-NITRO-PHENYL)-AMIDE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 4000, 0.1M NaAc, 0.1M NaCac, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.14 Å R-free 0.200
1RXK crystal structure of streptavidin mutant (M3) a combination of M1+M2 Deposited 2003-12-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–159（122 aa）
Chain B 38–159（122 aa）
Mutation：L124R Mutation：L124R BNI 5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID (4-NITRO-PHENYL)-AMIDE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.6；293 K；1.5M AS, 0.1M Tris-HCl, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.70 Å R-free 0.239
1SLD STREPTAVIDIN, PH 7.5, BOUND TO CYCLIC DISULFIDE-BONDED PEPTIDE LIGAND AC-CHPQFC-NH2 Deposited 1995-03-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 2.50 Å R-free 0.220
1SLE STREPTAVIDIN, PH 5.0, BOUND TO CYCLIC PEPTIDE AC-CHPQGPPC-NH2 Deposited 1995-03-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；pH 5.0
Resolution 2.00 Å R-free 0.233
1SLF APOSTREPTAVIDIN, PH 5.6, TWO MOLECULES OF (SO4)2 BOUND AT THE BIOTIN BINDING SITE Deposited 1995-03-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.6；pH 5.6
Resolution 1.76 Å R-free 0.202
1SLG STREPTAVIDIN, PH 5.6, BOUND TO PEPTIDE FCHPQNT Deposited 1995-03-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.6；pH 5.6
Resolution 1.76 Å R-free 0.222
1SRE CRYSTALLOGRAPHIC AND THERMODYNAMIC COMPARISON OF NATURAL AND SYNTHETIC LIGANDS BOUND TO STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded HAB 2-((4'-HYDROXYPHENYL)-AZO)BENZOIC ACID × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.78 Å
1SRE CRYSTALLOGRAPHIC AND THERMODYNAMIC COMPARISON OF NATURAL AND SYNTHETIC LIGANDS BOUND TO STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded HAB 2-((4'-HYDROXYPHENYL)-AZO)BENZOIC ACID × 8 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.78 Å
1SRF STRUCTURE-BASED DESIGN OF SYNTHETIC AZOBENZENE LIGANDS FOR STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded MTB 2-((3'-TERTBUTYL-4'-HYDROXYPHENYL)AZO)BENZOIC ACID × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1SRG STRUCTURE-BASED DESIGN OF SYNTHETIC AZOBENZENE LIGANDS FOR STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded MHB 2-((3'-METHYL-4'-HYDROXYPHENYL)AZO)BENZOIC ACID × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1SRG STRUCTURE-BASED DESIGN OF SYNTHETIC AZOBENZENE LIGANDS FOR STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded MHB 2-((3'-METHYL-4'-HYDROXYPHENYL)AZO)BENZOIC ACID × 8 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1SRH STRUCTURE-BASED DESIGN OF SYNTHETIC AZOBENZENE LIGANDS FOR STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded MOB 2-((3',5'-DIMETHOXY-4'-HYDROXYPHENYL)AZO)BENZOIC ACID × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.20 Å
1SRI STRUCTURE-BASED DESIGN OF SYNTHETIC AZOBENZENE LIGANDS FOR STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded DMB 2-((3',5'-DIMETHYL-4'-HYDROXYPHENYL)AZO)BENZOIC ACID × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.65 Å
1SRI STRUCTURE-BASED DESIGN OF SYNTHETIC AZOBENZENE LIGANDS FOR STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded DMB 2-((3',5'-DIMETHYL-4'-HYDROXYPHENYL)AZO)BENZOIC ACID × 8 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.65 Å
1SRJ STRUCTURE-BASED DESIGN OF SYNTHETIC AZOBENZENE LIGANDS FOR STREPTAVIDIN Deposited 1994-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–157（121 aa）
Chain B 37–157（121 aa）
Not recorded NAB 2-((4'-HYDROXYNAPHTHYL)-AZO)BENZOIC ACID × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1STP STRUCTURAL ORIGINS OF HIGH-AFFINITY BIOTIN BINDING TO STREPTAVIDIN Deposited 1992-03-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–183（159 aa）
Not recorded BTN BIOTIN × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.60 Å
1STR STREPTAVIDIN DIMERIZED BY DISULFIDE-BONDED PEPTIDE AC-CHPQNT-NH2 DIMER Deposited 1995-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å R-free 0.233
1STS STREPTAVIDIN DIMERIZED BY DISULFIDE-BONDED PEPTIDE FCHPQNT-NH2 DIMER Deposited 1995-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.95 Å R-free 0.238
1SWA APO-CORE-STREPTAVIDIN AT PH 4.5 Deposited 1997-03-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain B 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain C 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain D 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN WAS CRYSTALLIZED FROM 47% MPD (PH 4.5)
Resolution 1.90 Å R-free 0.256
1SWB APO-CORE-STREPTAVIDIN AT PH 7.5 Deposited 1997-03-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain B 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain C 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain D 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN WAS CRYSTALLIZED FROM 48% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5), 5.5H SOAKING IN 0.1M HEPES BUFFER PH 7.5
Resolution 1.85 Å R-free 0.253
1SWC APO-CORE-STREPTAVIDIN AT PH 4.5 Deposited 1997-03-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain B 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain C 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain D 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN WAS CRYSTALLIZED FROM 60% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5), SOAKING IN 20 MM SODIUM ACETATE BUFFER PH 4.5
Resolution 1.80 Å R-free 0.232
1SWD APO-CORE-STREPTAVIDIN IN COMPLEX WITH BIOTIN (TWO UNOCCUPIED BINDING SITES) AT PH 4.5 Deposited 1997-03-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain B 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain C 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain D 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Not recorded BTN BIOTIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN-BIOTIN COMPLEX WAS CO-CRYSTALLIZED FROM 50% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5) WITH 1.2M EXCESS OF BIOTIN
Resolution 1.90 Å R-free 0.328
1SWE APO-CORE-STREPTAVIDIN IN COMPLEX WITH BIOTIN AT PH 4.5 Deposited 1997-03-04 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain B 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain C 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Chain D 37–163（127 aa） Fragment：CORE, RESIDUES 13 - 139
Not recorded BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN-BIOTIN COMPLEX WAS CO-CRYSTALLIZED FROM 50% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5) WITH 2.5M EXCESS OF BIOTIN
Resolution 2.06 Å R-free 0.266
1SWF CIRCULAR PERMUTED STREPTAVIDIN E51/A46 Deposited 1997-04-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 75–163（89 aa）
Chain B 75–163（89 aa）
Chain C 75–163（89 aa）
Chain D 75–163（89 aa）
Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN WAS CRYSTALLIZED FROM 52% MPD (PH 4.5).
Resolution 2.00 Å R-free 0.288
1SWF CIRCULAR PERMUTED STREPTAVIDIN E51/A46 Deposited 1997-04-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 75–163（89 aa）
Chain B 75–163（89 aa）
Chain C 75–163（89 aa）
Chain D 75–163（89 aa）
Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN WAS CRYSTALLIZED FROM 52% MPD (PH 4.5).
Resolution 2.00 Å R-free 0.288
1SWG CIRCULAR PERMUTED STREPTAVIDIN E51/A46 IN COMPLEX WITH BIOTIN Deposited 1997-07-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 75–178（104 aa）
Chain B 75–178（104 aa）
Chain C 75–178（104 aa）
Chain D 75–178（104 aa）
Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN (12MG/ML, 10MM BIOTIN) WAS CRYSTALLIZED FROM 52% MPD (PH 4.5)
Resolution 1.80 Å R-free 0.245
1SWG CIRCULAR PERMUTED STREPTAVIDIN E51/A46 IN COMPLEX WITH BIOTIN Deposited 1997-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 75–178（104 aa）
Chain B 75–178（104 aa）
Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; BTN BIOTIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN (12MG/ML, 10MM BIOTIN) WAS CRYSTALLIZED FROM 52% MPD (PH 4.5)
Resolution 1.80 Å R-free 0.245
1SWG CIRCULAR PERMUTED STREPTAVIDIN E51/A46 IN COMPLEX WITH BIOTIN Deposited 1997-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 75–178（104 aa）
Chain D 75–178（104 aa）
Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; Mutation：;DELETION OF SURFACE LOOP RESIDUES 45 - 50 FROM THE SEQUENCE. THE OLD N- AND C-TERMINI (S139, A13, RESPECTIVELY) ARE CONNECTED INTRODUCING THE FOUR ADDITIONAL RESIDUES GGGS ; BTN BIOTIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN (12MG/ML, 10MM BIOTIN) WAS CRYSTALLIZED FROM 52% MPD (PH 4.5)
Resolution 1.80 Å R-free 0.245
1SWH CORE-STREPTAVIDIN MUTANT W79F AT PH 4.5 Deposited 1998-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W79F Mutation：W79F Mutation：W79F Mutation：W79F No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；pH 4.5
Resolution 1.70 Å R-free 0.244
1SWJ CORE-STREPTAVIDIN MUTANT W79F AT PH 4.5 Deposited 1998-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W79F Mutation：W79F Mutation：W79F Mutation：W79F No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；pH 4.5
Resolution 2.00 Å R-free 0.281
1SWK CORE-STREPTAVIDIN MUTANT W79F IN COMPLEX WITH BIOTIN AT PH 4.5 Deposited 1998-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W79F Mutation：W79F Mutation：W79F Mutation：W79F BTN BIOTIN × 3 BTQ EPI-BIOTIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；pH 4.5
Resolution 2.00 Å R-free 0.238
1SWL CORE-STREPTAVIDIN MUTANT W108F AT PH 7.0 Deposited 1998-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W108F Mutation：W108F Mutation：W108F Mutation：W108F No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 1.80 Å R-free 0.309
1SWN CORE-STREPTAVIDIN MUTANT W108F IN COMPLEX WITH BIOTIN AT PH 7.0 Deposited 1998-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W108F Mutation：W108F Mutation：W108F Mutation：W108F BTN BIOTIN × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 2.20 Å R-free 0.268
1SWO CORE-STREPTAVIDIN MUTANT W120F AT PH 7.5 Deposited 1998-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W120F Mutation：W120F Mutation：W120F Mutation：W120F No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 1.95 Å R-free 0.257
1SWP CORE-STREPTAVIDIN MUTANT W120F IN COMPLEX WITH BIOTIN AT PH 7.5 Deposited 1998-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W120F Mutation：W120F Mutation：W120F Mutation：W120F BTN BIOTIN × 3 BTQ EPI-BIOTIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 2.00 Å R-free 0.329
1SWQ CORE-STREPTAVIDIN MUTANT W120A AT PH 7.5 Deposited 1998-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W120A Mutation：W120A Mutation：W120A Mutation：W120A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 1.90 Å R-free 0.259
1SWR CORE-STREPTAVIDIN MUTANT W120A IN COMPLEX WITH BIOTIN AT PH 7.5 Deposited 1998-01-27 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：W120A Mutation：W120A Mutation：W120A Mutation：W120A BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 1.90 Å R-free 0.264
1SWT CORE-STREPTAVIDIN MUTANT D128A IN COMPLEX WITH BIOTIN AT PH 4.5 Deposited 1998-10-22 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Mutation：D128A Mutation：D128A BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；INCUBATED IN 2.5 M BIOTIN/HOH, COCRYSTALLIZED IN 52% MPD (PH 4.5)
Resolution 2.00 Å R-free 0.308
1SWU STREPTAVIDIN MUTANT Y43F Deposited 1998-10-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Mutation：Y43F Mutation：Y43F Mutation：Y43F Mutation：Y43F MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；pH 4.5
Resolution 1.14 Å R-free 0.157
1VWA STREPTAVIDIN-FSHPQNT Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4；SYNTHETIC MOTHER LIQUOR = 50 % SATURATED AMMONIUM SULFATE, 50 % 0.1 M POTASSIUM ACETATE., pH 4.0
Resolution 1.85 Å R-free 0.259
1VWB STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 11.8 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 11.8；SYNTHETIC MOTHER LIQUOR = 50 % SATURATED AMMONIUM SULFATE, 0.1 M CAPS ADJUSTED TO PH 11.8.
Resolution 1.82 Å R-free 0.239
1VWC STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 2.0 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 2；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.0.
Resolution 1.86 Å R-free 0.209
1VWD STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 3.0 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 3；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.0.
Resolution 1.87 Å R-free 0.209
1VWE STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 3.6 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.6；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.6.
Resolution 1.50 Å R-free 0.246
1VWF STREPTAVIDIN COMPLEXED WITH CYCLO-AC-[CHPQGPPC]-NH2 MONOMER, PH 3.67 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.67；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.67.
Resolution 1.92 Å R-free 0.236
1VWG STREPTAVIDIN COMPLEXED WITH THE HEAD-TO-TAIL DISULFIDE-BONDED PEPTIDE DIMER OF CYCLO-AC-[CHPQGPPC]-NH2, PH 2.5 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 2.5.
Resolution 1.46 Å R-free 0.237
1VWH STREPTAVIDIN COMPLEXED WITH THE HEAD-TO-TAIL DISULFIDE-BONDED PEPTIDE DIMER OF CYCLO-AC-[CHPQGPPC]-NH2, PH 3.5 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.5.
Resolution 1.48 Å R-free 0.239
1VWI STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 1.5, I222 COMPLEX Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 1.5.
Resolution 1.50 Å R-free 0.235
1VWI STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 1.5, I222 COMPLEX Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 1.5.
Resolution 1.50 Å R-free 0.235
1VWI STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 1.5, I222 COMPLEX Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 1.5.
Resolution 1.50 Å R-free 0.235
1VWJ STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 2.5, I222 COMPLEX Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.5
Resolution 1.45 Å R-free 0.237
1VWJ STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 2.5, I222 COMPLEX Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.5
Resolution 1.45 Å R-free 0.237
1VWK STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 3.0.
Resolution 1.45 Å R-free 0.240
1VWL STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 3.5, I222 COMPLEX Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.5.
Resolution 1.45 Å R-free 0.244
1VWM STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 4.2 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.2；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 4.2.
Resolution 1.60 Å R-free 0.243
1VWN STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 4.8 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.8；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 4.8.
Resolution 1.85 Å R-free 0.250
1VWO STREPTAVIDIN COMPLEXED WITH CYCLO-AC-[CHPQGPPC]-NH2 MONOMER, PH 2.85 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.85；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.85.
Resolution 1.65 Å R-free 0.228
1VWP STREPTAVIDIN COMPLEXED WITH CYCLO-AC-[CHPQGPPC]-NH2 MONOMER, PH 2.5 Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.5.
Resolution 1.75 Å R-free 0.235
1VWQ STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 2.5, I4122 COMPLEX Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.5.
Resolution 1.70 Å R-free 0.223
1VWR STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 3.5, I4122 COMPLEX Deposited 1997-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Not recorded LEA PENTANOIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.5.
Resolution 1.50 Å R-free 0.246
2BC3 T7-tagged full-length streptavidin Deposited 2005-10-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Not recorded GOL GLYCEROL × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 4.5；293 K；25% Ammonium Sulfate, 0.1M NaAcetate pH4.5, VAPOR DIFFUSION, temperature 293K
Resolution 1.54 Å R-free 0.243
2F01 Epi-biotin complex with core streptavidin Deposited 2005-11-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Not recorded BTN BIOTIN × 4 BTQ EPI-BIOTIN × 4 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 4.5；277 K；38% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.5, 0.2 M sodium chloride, VAPOR DIFFUSION, temperature 277K
Resolution 0.85 Å R-free 0.174
2G5L Streptavidin in complex with Nanotag Deposited 2006-02-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 37–163（127 aa） Fragment：residues 13-139
Chain B 37–163（127 aa） Fragment：residues 13-139
Not recorded SO4 SULFATE ION × 4 GOL GLYCEROL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.15 Å R-free 0.172
2GH7 Epi-biotin complex with core streptavidin Deposited 2006-03-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Not recorded BTN BIOTIN × 4 BTQ EPI-BIOTIN × 4 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；Protein solution - 10 mg/mL, 10mM Tris Hl, pH 7.0 Reservoir - 38% saturate ammonium sulfate, 0.1 M sodium acetate, pH 4.5,0.2 M NaCl , VAPOR DIFFUSION
Resolution 1.00 Å R-free 0.152
2IZA APOSTREPTAVIDIN PH 2.00 I4122 STRUCTURE Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–157（121 aa）
Not recorded FMT FORMIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2；SYNTHETIC MOTHER LIQUOR = 75% SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.00. COMPLEX PRODUCED BY SOAKING STREPTAVIDIN-2-IMINOBIOTIN CO-CRYSTAL.
Resolution 1.46 Å R-free 0.220
2IZB APOSTREPTAVIDIN PH 3.12 I4122 STRUCTURE Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–158（122 aa）
Not recorded SO4 SULFATE ION × 4 FMT FORMIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.12；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM ACETATE ADJUSTED TO PH 3.12. COMPLEX PRODUCED BY SOAKING STREPTAVIDIN-2-IMINOBIOTIN CO-CRYSTAL.
Resolution 1.20 Å R-free 0.235
2IZC APOSTREPTAVIDIN PH 2.0 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded NA SODIUM ION × 4 CL CHLORIDE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.0.
Resolution 1.40 Å R-free 0.241
2IZC APOSTREPTAVIDIN PH 2.0 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded NA SODIUM ION × 8 CL CHLORIDE ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.0.
Resolution 1.40 Å R-free 0.241
2IZD APOSTREPTAVIDIN pH 3.0 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded SO4 SULFATE ION × 2 CL CHLORIDE ION × 4 IOD IODIDE ION × 2 NH4 AMMONIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.0.
Resolution 1.60 Å R-free 0.252
2IZD APOSTREPTAVIDIN pH 3.0 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded SO4 SULFATE ION × 4 CL CHLORIDE ION × 8 IOD IODIDE ION × 4 NH4 AMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.0.
Resolution 1.60 Å R-free 0.252
2IZE APOSTREPTAVIDIN PH 3.08 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded NA SODIUM ION × 2 CL CHLORIDE ION × 4 FMT FORMIC ACID × 4 NH4 AMMONIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.08；SYNTHETIC MOTHER LIQUOR = 50 % SATURATED AMMONIUM SULFATE, 50 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 3.08.
Resolution 1.57 Å R-free 0.252
2IZE APOSTREPTAVIDIN PH 3.08 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded NA SODIUM ION × 4 CL CHLORIDE ION × 8 FMT FORMIC ACID × 8 NH4 AMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.08；SYNTHETIC MOTHER LIQUOR = 50 % SATURATED AMMONIUM SULFATE, 50 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 3.08.
Resolution 1.57 Å R-free 0.252
2IZF STREPTAVIDIN-BIOTIN PH 4.0 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded BTN BIOTIN × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 4.0.
Resolution 1.58 Å R-free 0.200
2IZG STREPTAVIDIN-BIOTIN PH 2.0 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded BTN BIOTIN × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.0.
Resolution 1.36 Å R-free 0.235
2IZH STREPTAVIDIN-BIOTIN PH 10.44 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 10.44；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 50.0 MM CAPS ADJUSTED TO PH 10.44.
Resolution 1.36 Å R-free 0.227
2IZI STREPTAVIDIN-BIOTIN PH 2.53 I4122 STRUCTURE Deposited 1997-08-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–159（123 aa）
Not recorded BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.53；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM ACETATE ADJUSTED TO PH 2.53. COMPLEX PRODUCED BY SOAKING STREPTAVIDIN-2-IMINOBIOTIN CO-CRYSTAL.
Resolution 1.50 Å R-free 0.246
2IZJ STREPTAVIDIN-BIOTIN PH 3.50 I4122 STRUCTURE Deposited 1997-08-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–159（123 aa）
Not recorded BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.5；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.50. COMPLEX PRODUCED BY SOAKING STREPTAVIDIN-2-IMINOBIOTIN CO-CRYSTAL.
Resolution 1.30 Å R-free 0.222
2IZK STREPTAVIDIN-GLYCOLURIL PH 2.58 I4122 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–159（123 aa）
Not recorded ACT ACETATE ION × 4 SO4 SULFATE ION × 4 GLL GLYCOLURIL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.58；SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25% 1.0M POTASSIUM ACETATE ADJUSTED TO PH 2.58. COMPLEX PRODUCED BY SOAKING STREPTAVIDIN-2-IMINOBIOTIN CO-CRYSTAL.
Resolution 1.30 Å R-free 0.202
2IZL STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX Deposited 1997-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 37–159（123 aa）
Chain D 37–159（123 aa）
Not recorded IMI 2-IMINOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.3；SYNTHETIC MOTHER LIQUOR = 75% SATURATED AMMONIUM SULFATE, 25% 1M TRIS ADJUSTED TO PH 7.3
Resolution 1.48 Å R-free 0.211
2QCB T7-tagged full-length streptavidin complexed with ruthenium ligand Deposited 2007-06-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：S112K KYS N-(4-{[(2-AMINOETHYL)AMINO]SULFONYL}PHENYL)-5-[(3AS,4S,6AR)-2-OXOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANAMIDE-(1,2,3,4,5,6-ETA)-BENZENE-CHLORO-RUTHENIUM(III) × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；protein, 26 mg/ml in water. 4-5x molar excess of ligand. reservoir, 1.0 M sodium citrate, 0.1 M cacodylate buffer, pH 6.5 , VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.65 Å R-free 0.187
2RTA APOSTREPTAVIDIN, PH 2.97, SPACE GROUP I4122 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–159（135 aa）
Not recorded SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.97；SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.97.
Resolution 1.39 Å R-free 0.238
2RTB APOSTREPTAVIDIN, PH 3.32, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded NA SODIUM ION × 2 ACT ACETATE ION × 2 CL CHLORIDE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.32；SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.32.
Resolution 1.50 Å R-free 0.258
2RTC APOSTREPTAVIDIN, PH 3.60, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.6；SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, SODIUM FORMATE ADJUSTED TO PH 3.60.
Resolution 1.50 Å R-free 0.265
2RTD STREPTAVIDIN-BIOTIN COMPLEX, PH 1.39, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.39；SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, SODIUM FORMATE ADJUSTED TO PH 1.39.
Resolution 1.65 Å R-free 0.236
2RTE STREPTAVIDIN-BIOTIN COMPLEX, PH 1.90, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded BTN BIOTIN × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.9；SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, SODIUM FORMATE ADJUSTED TO PH 1.90.
Resolution 1.50 Å R-free 0.220
2RTF STREPTAVIDIN-BIOTIN COMPLEX, PH 2.00, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded BTN BIOTIN × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2；ROOM TEMPERATURE, PH 2.0. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE, 0.99 MM BIOTIN, 170 MM 2-IMINOBIOTIN, ADJUSTED TO PH 2.00.
Resolution 1.47 Å R-free 0.230
2RTG STREPTAVIDIN-BIOTIN COMPLEX, PH 2.40, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded CL CHLORIDE ION × 2 BTN BIOTIN × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.4；ROOM TEMPERATURE, PH 2.0. SYNTHETIC MOTHER LIQUOR, 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE, 0.2 MM BIOTIN, 25 MM 2-IMINOBIOTIN, ADJUSTED TO PH 2.40.
Resolution 1.39 Å R-free 0.223
2RTH STREPTAVIDIN-GLYCOLURIL COMPLEX, PH 2.50, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded ACT ACETATE ION × 4 GLL GLYCOLURIL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.5；ROOM TEMPERATURE, PH 2.50. SYNTHETIC MOTHER LIQUOR, 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M POTASSIUM ACETATE, ADJUSTED TO PH 2.50.
Resolution 1.56 Å R-free 0.269
2RTH STREPTAVIDIN-GLYCOLURIL COMPLEX, PH 2.50, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded ACT ACETATE ION × 8 GLL GLYCOLURIL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.5；ROOM TEMPERATURE, PH 2.50. SYNTHETIC MOTHER LIQUOR, 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M POTASSIUM ACETATE, ADJUSTED TO PH 2.50.
Resolution 1.56 Å R-free 0.269
2RTI STREPTAVIDIN-GLYCOLURIL COMPLEX, PH 2.50, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded GLL GLYCOLURIL × 4 FMT FORMIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.9；SYNTHETIC MOTHER LIQUOR, 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE ADJUSTED TO PH 1.90.
Resolution 1.40 Å R-free 0.232
2RTI STREPTAVIDIN-GLYCOLURIL COMPLEX, PH 2.50, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded GLL GLYCOLURIL × 8 FMT FORMIC ACID × 12 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.9；SYNTHETIC MOTHER LIQUOR, 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE ADJUSTED TO PH 1.90.
Resolution 1.40 Å R-free 0.232
2RTJ STREPTAVIDIN-GLYCOLURIL COMPLEX, PH 2.50, SPACE GROUP I4122 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–159（135 aa）
Not recorded GLL GLYCOLURIL × 4 FMT FORMIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.5；ROOM TEMPERATURE, PH 2.50. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.50.
Resolution 1.40 Å R-free 0.221
2RTK STREPTAVIDIN-GLYCOLURIL COMPLEX, PH 2.58, SPACE GROUP I4122 PREPARED FROM AN APOSTREPTAVIDIN CRYSTAL Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–159（135 aa）
Not recorded ACT ACETATE ION × 4 SO4 SULFATE ION × 4 GLL GLYCOLURIL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.9；SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE ADJUSTED TO PH 1.90.
Resolution 1.82 Å R-free 0.276
2RTL STREPTAVIDIN-2-IMINOBIOTIN-SULFATE COMPLEX, PH 2.50, SPACE GROUP I4122 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–159（135 aa）
Not recorded SO4 SULFATE ION × 4 IMI 2-IMINOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 1.9；SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE ADJUSTED TO PH 1.90.
Resolution 1.41 Å R-free 0.234
2RTM STREPTAVIDIN-2-IMINOBIOTIN-SULFATE COMPLEX, PH 3.50, SPACE GROUP I4122 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–159（135 aa）
Not recorded SO4 SULFATE ION × 4 IMI 2-IMINOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.5；ROOM TEMPERATURE, PH 3.50. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 3.50.
Resolution 1.30 Å R-free 0.238
2RTN STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 2.0, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2；ROOM TEMPERATURE, PH 2.00. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE, SATURATED IN 2-IMINOBIOTIN, PH ADJUSTED TO PH 2.00.
Resolution 1.80 Å R-free 0.200
2RTN STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 2.0, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2；ROOM TEMPERATURE, PH 2.00. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE, SATURATED IN 2-IMINOBIOTIN, PH ADJUSTED TO PH 2.00.
Resolution 1.80 Å R-free 0.200
2RTO STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 2.6, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.6；ROOM TEMPERATURE, PH 2.60. SYNTHETIC MOTHER LIQUOR OF 50% SATURATED AMMONIUM SULFATE, 50% 1 M SODIUM FORMATE, 170 MM 2-IMINOBIOTIN, PH ADJUSTED TO 2.6.
Resolution 1.58 Å R-free 0.253
2RTO STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 2.6, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 2.6；ROOM TEMPERATURE, PH 2.60. SYNTHETIC MOTHER LIQUOR OF 50% SATURATED AMMONIUM SULFATE, 50% 1 M SODIUM FORMATE, 170 MM 2-IMINOBIOTIN, PH ADJUSTED TO 2.6.
Resolution 1.58 Å R-free 0.253
2RTP STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 3.25, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.25；ROOM TEMPERATURE, PH 3.25. SYNTHETIC MOTHER LIQUOR OF 50% SATURATED AMMONIUM SULFATE, 50% 1 M POTASSIUM ACETATE, 170 MM 2-IMINOBIOTIN, PH ADJUSTED TO 3.25
Resolution 1.50 Å R-free 0.243
2RTP STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 3.25, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.25；ROOM TEMPERATURE, PH 3.25. SYNTHETIC MOTHER LIQUOR OF 50% SATURATED AMMONIUM SULFATE, 50% 1 M POTASSIUM ACETATE, 170 MM 2-IMINOBIOTIN, PH ADJUSTED TO 3.25
Resolution 1.50 Å R-free 0.243
2RTQ STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 3.25, SPACE GROUP I222, CRYSTALLIZED FROM 4.3 M AMMONIUM SULFATE Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.25；ROOM TEMPERATURE, PH 3.25. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1 M POTASSIUM ACETATE, 25 MM 2-IMINOBIOTIN, PH ADJUSTED TO 3.25
Resolution 1.39 Å R-free 0.243
2RTQ STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 3.25, SPACE GROUP I222, CRYSTALLIZED FROM 4.3 M AMMONIUM SULFATE Deposited 1997-09-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.25；ROOM TEMPERATURE, PH 3.25. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1 M POTASSIUM ACETATE, 25 MM 2-IMINOBIOTIN, PH ADJUSTED TO 3.25
Resolution 1.39 Å R-free 0.243
2RTR STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 4.0, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4；ROOM TEMPERATURE, PH 4.00. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 4.0.
Resolution 1.62 Å R-free 0.234
2RTR STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 4.0, SPACE GROUP I222 Deposited 1997-09-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain B 25–159（135 aa）
Chain D 25–159（135 aa）
Not recorded IMI 2-IMINOBIOTIN × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4；ROOM TEMPERATURE, PH 4.00. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M POTASSIUM ACETATE ADJUSTED TO PH 4.0.
Resolution 1.62 Å R-free 0.234
2WPU Chaperoned ruthenium metallodrugs that recognize telomeric DNA Deposited 2009-08-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded KYT (3AS,4S,6AR)-4-(5-((3R,4R)-3,4-DIAMINOPYRROLIDIN-1-YL)-5-OXOPENTYL)TETRAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-2(3H)-ONE-P-CYMENE-CHLORO-RUTHENIUM(III) × 4 SO4 SULFATE ION × 4 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；PROTEIN, 26 MG/ML IN WATER. RESERVOIR, 2.0 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE BUFFER, PH 4.0 , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K. LIGAND SOAKING CONDITIONS: LIGAND, 10 MM K2OSO2(OH)4 IN 3.0 M AMMONIUM SULFATE, 0.1 SODIUM ACETATE BUFFER, PH 4.0, CRYO, 1.5 M AMMONIUM SULFATE, 0.1 SODIUM ACETATE BUFFER, PH 4.0
Resolution 1.92 Å R-free 0.198
2Y3E Traptavidin, apo-form Deposited 2010-12-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：RESIDUES 37-163
Chain B 37–163（127 aa） Fragment：RESIDUES 37-163
Mutation：YES Mutation：YES GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；12% PEG 8000, 9% ETHYLENE GLYCOL, 0.1 M HEPES PH 7.5
Resolution 1.45 Å R-free 0.178
2Y3F Traptavidin, biotin bound form Deposited 2010-12-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：RESIDUES 37-163
Mutation：YES BTN BIOTIN × 4 GOL GLYCEROL × 12 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；27% PEG 4000, 0.25M MGCL2, 0.1M TRIS-HCL PH 8.5
Resolution 1.49 Å R-free 0.151
3MG5 Core-streptavidin mutant F130L in complex with biotin Deposited 2010-04-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：CORE STREPTAVIDIN (RESIDUES 13-139)
Chain B 37–163（127 aa） Fragment：CORE STREPTAVIDIN (RESIDUES 13-139)
Chain C 37–163（127 aa） Fragment：CORE STREPTAVIDIN (RESIDUES 13-139)
Chain D 37–163（127 aa） Fragment：CORE STREPTAVIDIN (RESIDUES 13-139)
Mutation：F154L Mutation：F154L Mutation：F154L Mutation：F154L BTN BIOTIN × 4 GOL GLYCEROL × 11 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1 M HEPES, pH 7.5, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.30 Å R-free 0.178
3PK2 Artificial Transfer Hydrogenases for the Enantioselective Reduction of Cyclic Imines Deposited 2010-11-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：S112A 4IR {N-(4-{[2-(amino-kappaN)ethyl]sulfamoyl-kappaN}phenyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}(chloro)[(1,2,3,4,5-eta)-1,2,3,4,5-pentamethylcyclopentadienyl]iridium(III) × 4 IR3 IRIDIUM (III) ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；3.5 uL precipitation buffer (2.0 M ammonium sulfate, 0.1 M sodium acetate) mixed with 6.5 uL protein (26 mg/mL) and equilibrated against precipitation buffer, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.90 Å R-free 0.204
3RDM Crystal structure of R7-2 streptavidin complexed with biotin/PEG Deposited 2011-04-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S, W108V, L110T, F29L, S52G, R53S 1PE PENTAETHYLENE GLYCOL × 1 BTN BIOTIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.2 MgCl2, 0.1 Bis-Tris, pH 5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.60 Å R-free 0.245
3RDM Crystal structure of R7-2 streptavidin complexed with biotin/PEG Deposited 2011-04-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S, W108V, L110T, F29L, S52G, R53S 1PE PENTAETHYLENE GLYCOL × 4 BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.2 MgCl2, 0.1 Bis-Tris, pH 5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.60 Å R-free 0.245
3RDO Crystal structure of R7-2 streptavidin complexed with biotin Deposited 2011-04-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S, W108V, L110T, F29L, S52G, R53S BTN BIOTIN × 1 NI NICKEL (II) ION × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.1 M ammonium sulfate, 0.1 M Tris, pH 7.5, 20% PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.40 Å R-free 0.171
3RDQ Crystal structure of R7-2 streptavidin complexed with desthiobiotin Deposited 2011-04-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S, W108V, L110T, F29L, S52G, R53S DTB 6-(5-METHYL-2-OXO-IMIDAZOLIDIN-4-YL)-HEXANOIC ACID × 1 NI NICKEL (II) ION × 1 NA SODIUM ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；298 K；0.1 M Na-acetate, pH 5.0, 2 M Na-formate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.60 Å R-free 0.190
3RDS Crystal structure of the refolded R7-2 streptavidin Deposited 2011-04-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S, W108V, L110T, F29L, S52G, R53S 1PE PENTAETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；25% PEG 1500, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.50 Å R-free 0.214
3RDS Crystal structure of the refolded R7-2 streptavidin Deposited 2011-04-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S, W108V, L110T, F29L, S52G, R53S 1PE PENTAETHYLENE GLYCOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；25% PEG 1500, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.50 Å R-free 0.214
3RDU Crystal structure of R7-2 streptavidin complexed with PEG Deposited 2011-04-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S,W108V,L110T,F29L,S52G,R53S GOL GLYCEROL × 3 1PE PENTAETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；10% PEG1000, 10% PEG8000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.50 Å R-free 0.207
3RDU Crystal structure of R7-2 streptavidin complexed with PEG Deposited 2011-04-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S,W108V,L110T,F29L,S52G,R53S GOL GLYCEROL × 12 1PE PENTAETHYLENE GLYCOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；10% PEG1000, 10% PEG8000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.50 Å R-free 0.207
3RDX Crystal structure of ligand-free R7-2 streptavidin Deposited 2011-04-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Chain B 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S,W108V,L110T,F29L,S52G,R53S Mutation：T90S,W108V,L110T,F29L,S52G,R53S GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；298 K；0.1 M Na acetate, pH 4.5, 3 M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.278
3RDX Crystal structure of ligand-free R7-2 streptavidin Deposited 2011-04-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Chain B 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S,W108V,L110T,F29L,S52G,R53S Mutation：T90S,W108V,L110T,F29L,S52G,R53S GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；298 K；0.1 M Na acetate, pH 4.5, 3 M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.278
3RE5 Crystal structure of R4-6 streptavidin Deposited 2011-04-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S,W108V,L110T,F29L,R53S 1PE PENTAETHYLENE GLYCOL × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 MgCl2, 0.1 HEPES, pH 7.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.95 Å R-free 0.231
3RE5 Crystal structure of R4-6 streptavidin Deposited 2011-04-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S,W108V,L110T,F29L,R53S 1PE PENTAETHYLENE GLYCOL × 4 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 MgCl2, 0.1 HEPES, pH 7.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.95 Å R-free 0.231
3RE6 Crystal structure of R4-6 streptavidin Deposited 2011-04-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S,W108V,L110T,F29L,R53S GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；18% PEG 8000, 0.1 M Na-cacodylate, pH 6.5, 0.2 M Ca-acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.82 Å R-free 0.234
3RE6 Crystal structure of R4-6 streptavidin Deposited 2011-04-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–164（128 aa） Fragment：UNP Residues 37-164
Mutation：T90S,W108V,L110T,F29L,R53S GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；18% PEG 8000, 0.1 M Na-cacodylate, pH 6.5, 0.2 M Ca-acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.82 Å R-free 0.234
3RY1 Wild-type core streptavidin at atomic resolution Deposited 2011-05-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；298 K；52% MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.03 Å R-free 0.135
3RY2 Wild-type core streptavidin-biotin complex at atomic resolution Deposited 2011-05-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Not recorded BTN BIOTIN × 4 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；298 K；30% saturated ammonium sulfate, 0.1 M sodium acetate, 0.2 M sodium chloride, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 0.95 Å R-free 0.131
3T6F Biotin complex of Y54F core streptavidin Deposited 2011-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Chain B 37–163（127 aa） Fragment：UNP residues 37-163
Mutation：Y54F Mutation：Y54F BTN BIOTIN × 4 BSO BIOTIN-D-SULFOXIDE × 4 GOL GLYCEROL × 8 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；60% saturated ammonium sulfate, 5% isopropanol (30% glycerol cryoprotectant), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.22 Å R-free 0.151
3T6L Y54F mutant of core streptavidin Deposited 2011-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Mutation：Y54F CL CHLORIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.2；293 K；2.5 M sodium chloride, 0.1 M sodium-potassium phosphate (30% ethylene glycol cryoprotectant), pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.30 Å R-free 0.160
3WYP Crystal structure of wild-type core streptavidin in complex with D-biotin/biotin-D-sulfoxide at 1.3 A resolution Deposited 2014-09-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Chain B 37–163（127 aa） Fragment：UNP residues 37-163
Chain C 37–163（127 aa） Fragment：UNP residues 37-163
Chain D 37–163（127 aa） Fragment：UNP residues 37-163
Not recorded BTN BIOTIN × 2 GOL GLYCEROL × 10 BSO BIOTIN-D-SULFOXIDE × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.2；293 K；0.2M ammonium sulfate, 0.1M sodium acetate trihydrate, 24% polyethylene glycol 4000, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.30 Å R-free 0.179
3WYQ Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution Deposited 2014-09-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Chain B 37–163（127 aa） Fragment：UNP residues 37-163
Mutation：Y22S/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/Y83S/R84K/E101D/R103K/E116N BTN BIOTIN × 4 GOL GLYCEROL × 20 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.2；293 K；0.2M ammonium sulfate, 0.1M sodium acetate trihydrate, 24% polyethylene glycol 4000, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.00 Å R-free 0.163
3WZN Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin at 1.3 A resolution Deposited 2014-10-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Chain B 37–163（127 aa） Fragment：UNP residues 37-163
Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N BTN BIOTIN × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；294 K；100 mM sodium acetate trihydrate, 2.0 M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 294K
Resolution 1.30 Å R-free 0.207
3WZO Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin long tail (BTNtail) at 1.5 A resolution Deposited 2014-10-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Chain B 37–163（127 aa） Fragment：UNP residues 37-163
Chain C 37–163（127 aa） Fragment：UNP residues 37-163
Chain D 37–163（127 aa） Fragment：UNP residues 37-163
Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N ZOE 6-({5-[(3aS,4S,5S,6aR)-5-oxido-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid × 4 GOL GLYCEROL × 3 CD CADMIUM ION × 9 P6G HEXAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；293 K；0.1 M cadmium chloride hydrate, 0.1 M sodium acetate trihydrate, 30%(v/v) PEG400, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.50 Å R-free 0.236
3WZP Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with iminobiotin long tail (IMNtail) at 1.2 A resolution Deposited 2014-10-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Chain B 37–163（127 aa） Fragment：UNP residues 37-163
Chain C 37–163（127 aa） Fragment：UNP residues 37-163
Chain D 37–163（127 aa） Fragment：UNP residues 37-163
Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N ZOF 6-({5-[(2E,3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid × 4 GOL GLYCEROL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.2 M sodium citrate tribasic dehydrate, 0.1 M HEPES sodium, 35%(w/v) (+/-)-2-methyl-2, 4-pentandiol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.20 Å R-free 0.161
3WZQ Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with iminobiotin long tail (IMNtail) at 1.7 A resolution Deposited 2014-10-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Chain B 37–163（127 aa） Fragment：UNP residues 37-163
Chain C 37–163（127 aa） Fragment：UNP residues 37-163
Chain D 37–163（127 aa） Fragment：UNP residues 37-163
Mutation：Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N ZOF 6-({5-[(2E,3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid × 4 P6G HEXAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.8；293 K；60 mM sodium cacodylate trihydrate, 27%(w/v) PEG300, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.70 Å R-free 0.244
3X00 Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution Deposited 2014-10-09 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：UNP residues 37-163
Chain B 37–163（127 aa） Fragment：UNP residues 37-163
Chain C 37–163（127 aa） Fragment：UNP residues 37-163
Chain D 37–163（127 aa） Fragment：UNP residues 37-163
Mutation：Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N Mutation：Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N ZOF 6-({5-[(2E,3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid × 4 EDN ETHANE-1,2-DIAMINE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.4；293 K；0.2 M sodium fluoride, 20% PEG3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.30 Å R-free 0.188
4BX5 cis-divalent streptavidin Deposited 2013-07-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：RESIDUES 37-163
Chain B 37–163（127 aa） Fragment：RESIDUES 37-163
Chain C 37–163（127 aa） Fragment：RESIDUES 37-163
Chain D 37–163（127 aa） Fragment：RESIDUES 37-163
Mutation：YES Mutation：YES EDO 1,2-ETHANEDIOL × 4 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；2.5 % W/V POLYETHYLENE GLYCOL (PEG) 1000, 12.5 % W/V PEG 3350, 12.5 % V/V MPD, 30 MM OF ETHYLENE GLYCOL MIX (DI-ETHYLENEGLYCOL, TRI-ETHYLENEGLYCOL, TETRA-ETHYLENEGLYCOL, PENTA-ETHYLENEGLYCOL) AND 0.1 M MES/IMIDAZOLE PH 6.5, CORRESPONDING TO CONDITION E4 OF THE MORPHEUS SCREEN
Resolution 1.43 Å R-free 0.187
4BX6 trans-divalent streptavidin Deposited 2013-07-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：RESIDUES 37-163
Chain B 37–163（127 aa） Fragment：RESIDUES 37-163
Chain C 37–163（127 aa） Fragment：RESIDUES 37-163
Chain D 37–163（127 aa） Fragment：RESIDUES 37-163
Mutation：YES Mutation：YES MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；291 K；65% V/V 2-METHYL-2, 4-PENTANEDIOL (MPD), 0.1 M 2-(N-MORPHOLINO)ETHANESULFONIC ACID (MES) PH 6.0. CRYSTALS WERE OBTAINED BY THE SITTING-DROP VAPOR-DIFFUSION METHOD AT 291 K AND REACHED A MAXIMUM SIZE AFTER 10 DAYS AND WERE HARVESTED SOON AFTER
Resolution 1.59 Å R-free 0.187
4BX7 trans-divalent streptavidin bound to biotin-4-fluorescein Deposited 2013-07-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Mutation：YES MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 B4F biotin-4-fluorescein × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；277 K；65% V/V MPD, 0.1 M MES PH 4.0. CRYSTALS WERE OBTAINED BY THE SITTING-DROP VAPOR-DIFFUSION METHOD AT 277 K, REACHED A MAXIMUM SIZE AFTER 14 DAYS AND WERE HARVESTED SOON AFTER
Resolution 2.26 Å R-free 0.237
4CPE Wild-type streptavidin in complex with love-hate ligand 1 (LH1) Deposited 2014-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：RESIDUES 37-163
Chain B 37–163（127 aa） Fragment：RESIDUES 37-163
Not recorded LUV (3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4- d]imidazolidin-4-yl]-N-{2-[(2,6- diphenylphenyl)formamido]ethyl}pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；75% SATURATED AMMONIUM SULPHATE, 25% 1M SODIUM ACETATE PH4.5. SITTING DROP
Resolution 1.06 Å R-free 0.153
4CPF Wild-type streptavidin in complex with love-hate ligand 3 (LH3) Deposited 2014-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：RESIDUES 37-163
Chain B 37–163（127 aa） Fragment：RESIDUES 37-163
Not recorded LH3 methyl 4-(2-{5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H- thieno[3,4-d]imidazolidin-4-yl]pentanehydrazido}-3- [4-(methoxycarbonyl)phenyl]phenyl)benzoate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；75% SATURATED AMMONIUM SULPHATE, 25% 1M SODIUM ACETATE PH4.5. SITTING DROP
Resolution 1.14 Å R-free 0.165
4CPH trans-divalent streptavidin with love-hate ligand 4 Deposited 2014-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：RESIDUES 37-163
Chain B 37–163（127 aa） Fragment：RESIDUES 37-163
Chain C 37–163（127 aa） Fragment：RESIDUES 37-163
Chain D 37–163（127 aa） Fragment：RESIDUES 37-163
Mutation：YES Mutation：YES LH4 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4- d]imidazolidin-4-yl]-N'-{2,6-bis[4-(morpholine-4- sulfonyl)phenyl]phenyl}pentanehydrazide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；75% AMMONIUM SULPHATE, 25% SODIUM ACETATE PH4.5. SITTING DROP
Resolution 1.64 Å R-free 0.232
4CPI streptavidin A86D mutant with love-hate ligand 4 Deposited 2014-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 37–163（127 aa） Fragment：RESIDUES 37-163
Chain D 37–163（127 aa） Fragment：RESIDUES 37-163
Mutation：YES Mutation：YES LH4 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4- d]imidazolidin-4-yl]-N'-{2,6-bis[4-(morpholine-4- sulfonyl)phenyl]phenyl}pentanehydrazide × 4 PEG DI(HYDROXYETHYL)ETHER × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；CONDITION A9 OF THE MORPHEUS SCREEN: 0.1 M BICINE/TRIZMA BASE PH 8.5, 10% W/V POLYETHYLENE GLYCOL 20,000, 20% V/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 30 MM MAGNESIUM CHLORIDE AND 30 MM CALCIUM CHLORIDE. SITTING DROP.
Resolution 1.54 Å R-free 0.181
4CPI streptavidin A86D mutant with love-hate ligand 4 Deposited 2014-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa） Fragment：RESIDUES 37-163
Chain B 37–163（127 aa） Fragment：RESIDUES 37-163
Mutation：YES Mutation：YES LH4 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4- d]imidazolidin-4-yl]-N'-{2,6-bis[4-(morpholine-4- sulfonyl)phenyl]phenyl}pentanehydrazide × 4 PEG DI(HYDROXYETHYL)ETHER × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；CONDITION A9 OF THE MORPHEUS SCREEN: 0.1 M BICINE/TRIZMA BASE PH 8.5, 10% W/V POLYETHYLENE GLYCOL 20,000, 20% V/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 30 MM MAGNESIUM CHLORIDE AND 30 MM CALCIUM CHLORIDE. SITTING DROP.
Resolution 1.54 Å R-free 0.181
4CPI streptavidin A86D mutant with love-hate ligand 4 Deposited 2014-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 37–163（127 aa） Fragment：RESIDUES 37-163
Chain D 37–163（127 aa） Fragment：RESIDUES 37-163
Mutation：YES Mutation：YES LH4 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4- d]imidazolidin-4-yl]-N'-{2,6-bis[4-(morpholine-4- sulfonyl)phenyl]phenyl}pentanehydrazide × 4 PEG DI(HYDROXYETHYL)ETHER × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；CONDITION A9 OF THE MORPHEUS SCREEN: 0.1 M BICINE/TRIZMA BASE PH 8.5, 10% W/V POLYETHYLENE GLYCOL 20,000, 20% V/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 30 MM MAGNESIUM CHLORIDE AND 30 MM CALCIUM CHLORIDE. SITTING DROP.
Resolution 1.54 Å R-free 0.181
4DNE Crystal structure of a triple-mutant of streptavidin in complex with desthiobiotin Deposited 2012-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Mutation：E44V, S45T, V47R Mutation：E44V, S45T, V47R DTB 6-(5-METHYL-2-OXO-IMIDAZOLIDIN-4-YL)-HEXANOIC ACID × 4 SO4 SULFATE ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；10 mM MgSO4, 50 mM Na caodylate, 2M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.88 Å R-free 0.219
4EKV Streptavidin 8-aa-loop H127C mutein with reversible biotin binding Deposited 2012-04-09 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–183（159 aa）
Mutation：H151C BTN BIOTIN × 4 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；50% Tacsimate, 10% glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.225
4GD9 Circular Permuted Streptavidin N49/G48 Deposited 2012-07-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 73–163（91 aa）
Chain A 37–72（36 aa）
Chain B 73–163（91 aa）
Chain B 37–72（36 aa）
Chain C 73–163（91 aa）
Chain C 37–72（36 aa）
Chain D 73–163（91 aa）
Chain D 37–72（36 aa）
Not recorded BTN BIOTIN × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；1.0 M ammonium phosphate, 0.1 M Tris-chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.50 Å R-free 0.230
4GDA Circular Permuted Streptavidin A50/N49 Deposited 2012-07-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 74–163（90 aa）
Chain A 37–73（37 aa）
Chain B 74–163（90 aa）
Chain B 37–73（37 aa）
Not recorded BTN BIOTIN × 4 GOL GLYCEROL × 6 SO4 SULFATE ION × 6 EOH ETHANOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；25% saturated ammonium sulfate, 0.1 M Tris-chloride, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.00 Å R-free 0.143
4GJS Streptavidin-K121H Deposited 2012-08-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–183（147 aa）
Chain B 37–183（147 aa）
Not recorded 0OD trichloro{(1,2,3,4,5-eta)-1,2,3,4-tetramethyl-5-[2-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)ethyl]cyclopentadienyl}rhodium(1+) × 4 RH Rhodium × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1 M MES, 19 % PEG500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.250
4GJV Streptavidin-S112H Deposited 2012-08-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–183（147 aa）
Mutation：S112H 0OD trichloro{(1,2,3,4,5-eta)-1,2,3,4-tetramethyl-5-[2-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)ethyl]cyclopentadienyl}rhodium(1+) × 4 CL CHLORIDE ION × 16 RH Rhodium × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1 M MES, 19 % PEG500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.40 Å R-free 0.230
4IRW Co-crystallization of streptavidin-biotin complex with a lanthanide-ligand complex gives rise to a novel crystal form Deposited 2013-01-15 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 36–163（128 aa） Fragment：UNP residues 36-163
Not recorded PDC PYRIDINE-2,6-DICARBOXYLIC ACID × 48 BTN BIOTIN × 4 TB TERBIUM(III) ION × 20 NA SODIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；1.5 uL protein (pH 8.0) incubated with saturated biotin solution + 1.5 uL 200 mM Na3[Tb(Dpa)3] + 3 uL 60% v/v MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.40 Å R-free 0.154
4JO6 Streptavidin complex with SBP-Tag Deposited 2013-03-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 25–183（159 aa） Fragment：UNP residues 25-183
Chain B 25–183（159 aa） Fragment：UNP residues 25-183
Chain C 25–183（159 aa） Fragment：UNP residues 25-183
Chain D 25–183（159 aa） Fragment：UNP residues 25-183
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；56% Tacsimate, 12%(w/v) glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.75 Å R-free 0.242
4OKA Structural-, Kinetic- and Docking Studies of Artificial Imine Reductases Based on the Biotin-Streptavidin Technology: An Induced Lock-and-Key Hypothesis Deposited 2014-01-22 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa） Fragment：UNP residues 38-183
Mutation：S112K 5IR [N-(4-{[2-(amino-kappaN)ethyl]sulfamoyl-kappaN}phenyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamidato]iridium(III) × 4 IR IRIDIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；ammonium sulfate, sodium acetate, pH 8.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.50 Å R-free 0.240
4Y59 Crystal structure of ALiS1-Streptavidin complex Deposited 2015-02-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa） Fragment：UNP residues 39-159
Chain B 39–159（121 aa） Fragment：UNP residues 39-159
Chain C 39–159（121 aa） Fragment：UNP residues 39-159
Chain D 39–159（121 aa） Fragment：UNP residues 39-159
Not recorded T21 2-[3-(trifluoromethyl)phenyl]furo[3,2-c]pyridin-4(5H)-one × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1M Hepes-NaOH (pH7.0), 50%(v/v) MPD
Resolution 1.22 Å R-free 0.155
4Y5D CRYSTAL STRUCTURE OF ALiS2-STREPTAVIDIN COMPLEX Deposited 2015-02-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–160（122 aa） Fragment：UNP residues 39-160
Chain B 39–160（122 aa） Fragment：UNP residues 39-160
Chain C 39–160（122 aa） Fragment：UNP residues 39-160
Chain D 39–160（122 aa） Fragment：UNP residues 39-160
Not recorded MT6 methyl 3-(4-oxo-4,5-dihydrofuro[3,2-c]pyridin-2-yl)benzoate × 3 PE3 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 P6G HEXAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1M Mes-NaOH (pH6.0), 45%(w/v) PEG1000
Resolution 1.20 Å R-free 0.174
4YVB Structure of D128N streptavidin Deposited 2015-03-19 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 40–159（120 aa）
Chain B 40–159（120 aa）
Chain C 40–159（120 aa）
Chain D 40–159（120 aa）
Mutation：D128N Mutation：D128N Mutation：D128N Mutation：D128N BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.05 M HEPES, pH 7.5 with 7.5% PEG 8000
Resolution 1.35 Å R-free 0.170
5B5F Crystal structure of ALiS3-Streptavidin complex Deposited 2016-05-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 40–159（120 aa） Fragment：UNP residues 40-159
Chain B 40–159（120 aa） Fragment：UNP residues 40-159
Chain C 40–159（120 aa） Fragment：UNP residues 40-159
Chain D 40–159（120 aa） Fragment：UNP residues 40-159
Not recorded 6F3 N-methyl-3-(4-oxo-4,5-dihydrofuro[3,2-c]pyridin-2-yl)benzenesulfonamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1M MES-NaOH (pH6.0), 35%(w/v) PEG 1000, 2.0% agarose hydrogel
Resolution 1.20 Å R-free 0.193
5B5G Crystal structure of ALiS4-Streptavidin complex Deposited 2016-05-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 40–159（120 aa） Fragment：UNP residues 40-159
Chain B 40–159（120 aa） Fragment：UNP residues 40-159
Chain C 40–159（120 aa） Fragment：UNP residues 40-159
Chain D 40–159（120 aa） Fragment：UNP residues 40-159
Not recorded SO3 SULFITE ION × 7 6FX methyl 5-(4-oxidanylidene-5~{H}-furo[3,2-c]pyridin-2-yl)pyridine-3-carboxylate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.1M Tris-HCl (pH8.0), 30%(w/v) PEG 1000, 2.0% agarose hydrogel
Resolution 1.50 Å R-free 0.193
5CSE Streptavidin-S112Y-K121E Complexed with Palladium-Containing Biotin Ligand Deposited 2015-07-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–158（121 aa） Fragment：UNP residues 37-158
Chain B 38–158（121 aa） Fragment：UNP residues 37-158
Mutation：S112Y-K121E Mutation：S112Y-K121E SVP chloro{di-tert-butyl[2-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)ethyl]-lambda~5~-phosphanyl}(1-phenylprop-1-ene-1,3-diyl-kappa~2~C~1~,C~3~)palladium × 4 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；15-25% PEG 1500, 100mM SPG buffer (mixed succinic acid, sodium dihydrogen phosphate and glycine in the ratio 2:7:7; 75% at pH 4 and 25% at pH 10)
Resolution 1.79 Å R-free 0.256
5CSE Streptavidin-S112Y-K121E Complexed with Palladium-Containing Biotin Ligand Deposited 2015-07-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 38–158（121 aa） Fragment：UNP residues 37-158
Chain B 38–158（121 aa） Fragment：UNP residues 37-158
Mutation：S112Y-K121E Mutation：S112Y-K121E SVP chloro{di-tert-butyl[2-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)ethyl]-lambda~5~-phosphanyl}(1-phenylprop-1-ene-1,3-diyl-kappa~2~C~1~,C~3~)palladium × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；15-25% PEG 1500, 100mM SPG buffer (mixed succinic acid, sodium dihydrogen phosphate and glycine in the ratio 2:7:7; 75% at pH 4 and 25% at pH 10)
Resolution 1.79 Å R-free 0.256
5F2B Expanding Nature's Catalytic Repertoire -Directed Evolution of an Artificial Metalloenzyme for In Vivo Metathesis Deposited 2015-12-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：V47A,N49K,T114Q,A119G,K121R 9RU [1-[4-[[5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]methyl]-2,6-dimethyl-phenyl]-3-(2,4,6-trimethylphenyl)-4,5-dihydroimidazol-1-ium-2-yl]-bis(chloranyl)ruthenium × 4 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；1.5 M ammonium sulfate, 0.1 M sodium acetate
Resolution 1.70 Å R-free 0.206
5JD2 SFX structure of corestreptavidin-selenobiotin complex Deposited 2016-04-15 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 40–159（120 aa）
Chain B 40–159（120 aa）
Chain C 40–159（120 aa）
Chain D 40–159（120 aa）
Not recorded BYY 5-[(3aS,4S,6aR)-2-oxohexahydro-1H-selenopheno[3,4-d]imidazol-4-yl]pentanoic acid × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；293 K；24% PEG 1500 and 20% glycerol
Resolution 1.90 Å R-free 0.200
5K67 Designed Artificial Cupredoxins Deposited 2016-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded SI4 [CuII(biot-pr-dpea)]2+ × 4 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；274 K；2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4
Resolution 1.70 Å R-free 0.234
5K68 Designed Artificial Cupredoxins Deposited 2016-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded SI9 [CuII(biot-bu-dpea)]2+ × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；274 K；2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4
Resolution 1.40 Å R-free 0.181
5L3Y Designed Artificial Cupredoxins Deposited 2016-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded CU6 [CuII(biot-et-dpea)]2+ × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；274 K；2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4
Resolution 1.70 Å R-free 0.244
5N7X CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK Deposited 2017-02-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–183（183 aa）
Chain K 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1M Magnesium acetate 0.1M Sodium cacodylate pH 6.5 15% PEG6000
Resolution 1.12 Å R-free 0.186
5N7X CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK Deposited 2017-02-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–183（183 aa）
Chain M 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1M Magnesium acetate 0.1M Sodium cacodylate pH 6.5 15% PEG6000
Resolution 1.12 Å R-free 0.186
5N7X CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK Deposited 2017-02-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain E 1–183（183 aa）
Chain O 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1M Magnesium acetate 0.1M Sodium cacodylate pH 6.5 15% PEG6000
Resolution 1.12 Å R-free 0.186
5N7X CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK Deposited 2017-02-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain F 1–183（183 aa）
Chain H 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1M Magnesium acetate 0.1M Sodium cacodylate pH 6.5 15% PEG6000
Resolution 1.12 Å R-free 0.186
5N89 CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE GNSFDDWLASKG Deposited 2017-02-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Chain D 1–183（183 aa）
Chain F 1–183（183 aa）
Not recorded GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M Potassium chloride 0.1M HEPES pH 7.5 15% PEG6000
Resolution 1.27 Å R-free 0.204
5N89 CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE GNSFDDWLASKG Deposited 2017-02-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain H 1–183（183 aa）
Chain K 1–183（183 aa）
Chain M 1–183（183 aa）
Chain O 1–183（183 aa）
Not recorded GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M Potassium chloride 0.1M HEPES pH 7.5 15% PEG6000
Resolution 1.27 Å R-free 0.204
5N8B CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE AFPDYLAEYHGG Deposited 2017-02-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Chain D 1–183（183 aa）
Chain G 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.1M Magnesium chloride 0.1M Sodium citrate pH 5 15% PEG4000
Resolution 1.03 Å R-free 0.155
5N8E CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE RDPAPAWAHGGG Deposited 2017-02-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Chain C 1–183（183 aa）
Chain D 1–183（183 aa）
Not recorded NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；0.2M Ammonium acetate 0.1M Sodium acetate pH 4 15% PEG4000
Resolution 1.10 Å R-free 0.153
5N8J CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE D-amino acid containing peptide GyGlanvdessG Deposited 2017-02-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Chain C 1–183（183 aa）
Chain D 1–183（183 aa）
Not recorded IPA ISOPROPYL ALCOHOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1M Sodium citrate pH 5.6 20% PEG4000 20% Isopropanol
Resolution 1.05 Å R-free 0.193
5N8T CRYSTAL STRUCTURE OF STREPTAVIDIN D-amino acid containing peptide Gdlwqheatwkkq Deposited 2017-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–183（183 aa）
Not recorded DLE D-LEUCINE × 1 DTR D-TRYPTOPHAN × 2 DGN D-GLUTAMINE × 1 DHI D-HISTIDINE × 1 DGL D-GLUTAMIC ACID × 1 DAL D-ALANINE × 1 DTH D-THREONINE × 1 DLY D-LYSINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；0.1M Sodium citrate pH 4.5, 20% PEG4000
Resolution 1.61 Å R-free 0.243
5N8W CRYSTAL STRUCTURE OF STREPTAVIDIN with D-amino acid containing peptide GGwhdeatwkpG Deposited 2017-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M Magnesium acetate 0.1M MOPS pH 7.5 12% PEG8000
Resolution 1.10 Å R-free 0.151
5N99 CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ Deposited 2017-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Chain D 1–183（183 aa）
Chain M 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；0.1M Sodium citrate pH 5.5 15% PEG6000
Resolution 1.50 Å R-free 0.192
5N99 CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ Deposited 2017-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–183（183 aa）
Chain I 1–183（183 aa）
Chain K 1–183（183 aa）
Chain Q 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；0.1M Sodium citrate pH 5.5 15% PEG6000
Resolution 1.50 Å R-free 0.192
5N99 CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ Deposited 2017-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain O 1–183（183 aa）
Chain S 1–183（183 aa）
Chain U 1–183（183 aa）
Chain Y 1–183（183 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；0.1M Sodium citrate pH 5.5 15% PEG6000
Resolution 1.50 Å R-free 0.192
5TO2 Crystal structure of streptavidin with one wild type subunit and three mutated subunits (N23A/S27D/S45A) Deposited 2016-10-16 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–163（125 aa）
Chain B 39–163（125 aa）
Chain C 39–163（125 aa）
Chain D 39–162（124 aa）
Mutation：N23A, S27D, S45A Mutation：N23A, S27D, S45A Mutation：N23A, S27D, S45A PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M Tris pH 8.3, 0.25 M MgCl2, 32% PEG4K
Resolution 1.65 Å R-free 0.234
5VCQ A Hyrdrogen Producing Hybrid Streptavidin-Diiron Catalyst Deposited 2017-03-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Chain C 1–183（183 aa）
Chain D 1–183（183 aa）
Not recorded BN7 2-methylpropyl 5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；100mM TRIS pH 8.5, 200mM MgCl2, 20-30% PEG4000
Resolution 2.05 Å R-free 0.218
5VKX Coordination Chemistry within a Protein Host: Regulation of the Secondary Coordination Sphere Deposited 2017-04-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded S18 [N-(3-{bis[2-(pyridin-2-yl-kappaN)ethyl]amino-kappaN}ethyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide](azido)copper × 4 CU COPPER (II) ION × 4 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL protein, 2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.37 Å R-free 0.187
5VL5 Coordination Chemistry within a Protein Host: Regulation of the Secondary Coordination Sphere Deposited 2017-04-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded S31 [N-(3-{bis[2-(pyridin-2-yl-kappaN)ethyl]amino-kappaN}propyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide](azido)(hydroxy)copper × 4 CU COPPER (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.46 Å R-free 0.260
5VL8 Coordination Chemistry within a Protein Host: Regulation of the Secondary Coordination Sphere Deposited 2017-04-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded S32 [N-(3-{bis[2-(pyridin-2-yl-kappaN)ethyl]amino-kappaN}propyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide](hydroxy)copper × 4 CU COPPER (II) ION × 4 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL protein, 2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.70 Å R-free 0.215
5WBA Peroxide Activation Regulated by Hydrogen Bonds within Artificial Cu Proteins - WT Deposited 2017-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa） Fragment：UNP residues 38-183
Not recorded SI8 [N-(2-{bis[2-(pyridin-2-yl-kappaN)ethyl]amino-kappaN}ethyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide](hydrogen peroxido-kappaO)copper × 4 ACT ACETATE ION × 4 SO4 SULFATE ION × 4 CU COPPER (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；0.1 M sodium acetate, pH 4.0, 2.6 M ammonium sulfate
Resolution 1.50 Å R-free 0.151
5WBB Peroxide Activation Regulated by Hydrogen Bonds within Artificial Cu Proteins - S112A Deposited 2017-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa） Fragment：UNP residues 38-183
Mutation：S112A SQ1 [N-(2-{bis[2-(pyridin-2-yl-kappaN)ethyl]amino-kappaN}ethyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide]copper × 4 CU COPPER (II) ION × 4 GOL GLYCEROL × 4 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；0.1 M sodium acetate, 2.6 M sodium sulfate
Resolution 1.50 Å R-free 0.188
5WBC Designed Artificial Cupredoxins - WT Deposited 2017-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded CU6 [CuII(biot-et-dpea)]2+ × 4 GOL GLYCEROL × 4 ACT ACETATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；274 K；0.1 M sodium acetate, pH 4, 2.6 M ammonium sulfate
Resolution 1.72 Å R-free 0.168
5WBD Peroxide Activation Regulated by Hydrogen Bonds within Artificial Cu Proteins - N49A Deposited 2017-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa） Fragment：UNP residues 38-183
Mutation：N49A SI7 [N-(2-{bis[2-(pyridin-2-yl-kappaN)ethyl]amino-kappaN}ethyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide](hydroxy)copper × 4 ACT ACETATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；0.1 M sodium acetate, 2.0 M sodium sulfate
Resolution 1.50 Å R-free 0.178
6ANX Peroxide Activation Regulated by Hydrogen Bonds within Artificial Cu Proteins - WT (low exposure) Deposited 2017-08-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa） Fragment：UNP residues 38-183
Not recorded SI0 [N-(2-{bis[2-(pyridin-2-yl-kappaN)ethyl]amino-kappaN}ethyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide](hydrogen peroxido-kappaO)hydroxycopper × 4 ACT ACETATE ION × 4 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；274 K；2.6 M ammonium sulfate, 0.1 M sodium acetate
Resolution 1.62 Å R-free 0.187
6AUC Artificial metalloproteins containing a Co4O4 active site - 2xm-Sav Deposited 2017-08-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, K121A OLS N-biotin-C-Co4(mu3-O)4(Py)4(H2O)4-beta-alanine × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；2.6 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.46 Å R-free 0.201
6AUE Artificial Metalloproteins Containing a Co4O4 Active Site - 2xm-S112Y-b Deposited 2017-08-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112Y, K121A OL3 N-biotin-C-Co4(mu3-O)4(OAc)(Py)4(H2O)3-beta-alanine × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；2.6 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.36 Å R-free 0.229
6AUH Artificial Metalloproteins Containing a Co4O4 Active Site - 2xm-S112Y-a Deposited 2017-09-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112Y, K121A OL5 N-biotin-C-Co4(mu3-O)4(OAc)(Py)3(H2O)3-beta-alanine × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；2.6 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.60 Å R-free 0.219
6AUL Artificial Metalloproteins Containing a Co4O4 Active Site - 2xm-S112Y-b Deposited 2017-09-01 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112Y, K121A BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；2.6 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.36 Å R-free 0.195
6AUO Artificial Metalloproteins Containing a Co4O4 Active Site - 2xm-S112F Deposited 2017-09-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112F, K121A OL4 N-biotin-C-Co4(mu3-O)4(Py)3(H2O)4-beta-alanine × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；2.6 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.70 Å R-free 0.222
6AVK Streptavidin bound to peptide-like compound KPM-6 Deposited 2017-09-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–159（123 aa）
Chain B 37–159（123 aa）
Not recorded BZ4 N-[(2H-1,3-benzodioxol-5-yl)methyl]-2-({[(2H-1,3-benzodioxol-5-yl)methyl][2-(chloromethyl)-1,3-oxazole-4-carbonyl]amino}methyl)-N-[(4-carbamoyl-1,3-oxazol-2-yl)methyl]-1,3-oxazole-4-carboxamide × 2 HDO 1-hydroxydodecan-4-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；290 K；0.2 M potassium iodide, 20% PEG3350
Resolution 1.40 Å R-free 0.186
6ESS Artificial imine reductase mutant S112A-N118P-K121A-S122M Deposited 2017-10-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded 4IR {N-(4-{[2-(amino-kappaN)ethyl]sulfamoyl-kappaN}phenyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}(chloro)[(1,2,3,4,5-eta)-1,2,3,4,5-pentamethylcyclopentadienyl]iridium(III) × 4 IR IRIDIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.91 Å R-free 0.327
6ESU Artificial imine reductase mutant S112A-N118P-K121A-S122M Deposited 2017-10-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded 6IR 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[4-(2-azanylethylsulfamoyl)phenyl]pentanamide × 4 IR IRIDIUM ION × 16 ACT ACETATE ION × 4 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.78 Å R-free 0.211
6FH8 E. coli surface display of streptavidin for directed evolution of an allylic deallocase Deposited 2018-01-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：S112M-K121A JCT biotinylated ruthenium cyclopentadienide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.64 Å R-free 0.159
6FRY Photo-Driven Hydrogen Evolution by an Artificial Hydrogenase Utilizing the Biotin-Streptavidin Technology Deposited 2018-02-16 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded 9CO [CoBr(appy)-Biot]Br × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0
Resolution 1.70 Å R-free 0.202
6GH7 WILDTYPE CORE-STREPTAVIDIN WITH a conjugated BIOTINYLATED PYRROLIDINE Deposited 2018-05-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Not recorded EYW 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[(3~{R})-pyrrolidin-3-yl]pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；25 mg/mL protein in deionised water is mixed at equal volume with 51% MPD.
Resolution 1.08 Å R-free 0.185
6GMI Genetic Engineering of an Artificial Metalloenzyme for Transfer Hydrogenation of a Self-Immolative Substrate in E. coli's Periplasm. Deposited 2018-05-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Mutation：S112V, E116SPLSEALTKANSPAEAYKASRGAGA, K121A 4IR {N-(4-{[2-(amino-kappaN)ethyl]sulfamoyl-kappaN}phenyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}(chloro)[(1,2,3,4,5-eta)-1,2,3,4,5-pentamethylcyclopentadienyl]iridium(III) × 4 IR3 IRIDIUM (III) ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；0.1 M SPG 9 pH (Buffer) 25 %w/v PEG 1500 (Precipitant)
Resolution 1.60 Å R-free 0.199
6J6J Biotin-bound streptavidin Deposited 2019-01-15 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 40–158（119 aa）
Chain B 40–158（119 aa）
Chain C 40–158（119 aa）
Chain D 40–158（119 aa）
Not recorded BTN BIOTIN × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6K Apo-state streptavidin Deposited 2019-01-15 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 40–158（119 aa）
Chain B 40–158（119 aa）
Chain C 40–158（119 aa）
Chain D 40–158（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6LNG Rapid crystallization of streptavidin using charged peptides Deposited 2019-12-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–163（125 aa）
Chain B 39–163（125 aa）
Chain C 39–163（125 aa）
Chain D 39–163（125 aa）
Not recorded GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v polyethylene glycol monomethyl ether 5000
Resolution 1.80 Å R-free 0.219
6LNG Rapid crystallization of streptavidin using charged peptides Deposited 2019-12-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 39–163（125 aa）
Chain F 39–163（125 aa）
Not recorded GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v polyethylene glycol monomethyl ether 5000
Resolution 1.80 Å R-free 0.219
6M9B Wild-type streptavidin in complex with biotin solved by native SAD with data collected at 6 keV Deposited 2018-08-23 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Not recorded BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；13.25mg SAV WT + 0.610mg biotin in 500uL H2O in the well: 500uL => 50% MPD sitting drops: 1uL SAV-biot + 1uL 80% MPD, 100mM MMT pH 5.5
Resolution 1.55 Å R-free 0.183
6QBB Engineered streptavidin variant (ENAGY) in complex with the Strep-tag II peptide Deposited 2018-12-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 38–163（126 aa）
Chain B 38–163（126 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG MME 550, zinc sulfate, 2-(N-morpholino)ethanesulfonic acid
Resolution 1.52 Å R-free 0.190
6QSY Engineered streptavidin variant (H--WY) in complex with the Strep-tag II peptide Deposited 2019-02-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 38–163（126 aa）
Not recorded PGE TRIETHYLENE GLYCOL × 12 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；293 K；polyethylene glycol 8000, lithium sulfate, tris(hydroxymethyl)aminomethane
Resolution 1.70 Å R-free 0.179
6QW4 Engineered streptavidin variant (ACGR) in complex with the Strep-tag II peptide Deposited 2019-03-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 38–163（126 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；293 K；lithium sulfate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Resolution 2.10 Å R-free 0.240
6S4Q scdSav(SASK) - Engineering Single-Chain Dimeric Streptavidin as Host for Artificial Metalloenzymes Deposited 2019-06-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Not recorded 4IR {N-(4-{[2-(amino-kappaN)ethyl]sulfamoyl-kappaN}phenyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}(chloro)[(1,2,3,4,5-eta)-1,2,3,4,5-pentamethylcyclopentadienyl]iridium(III) × 4 GOL GLYCEROL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4
Resolution 1.85 Å R-free 0.231
6S50 scdSav(SARK)mv2 - Engineering Single-Chain Dimeric Streptavidin as Host for Artificial Metalloenzymes Deposited 2019-06-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Not recorded 4IR {N-(4-{[2-(amino-kappaN)ethyl]sulfamoyl-kappaN}phenyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}(chloro)[(1,2,3,4,5-eta)-1,2,3,4,5-pentamethylcyclopentadienyl]iridium(III) × 4 GOL GLYCEROL × 6 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4
Resolution 2.00 Å R-free 0.208
6SOK Engineered streptavidin variant (VTAR) in complex with the Twin-Strep-tag peptide Deposited 2019-08-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 38–163（126 aa）
Chain B 38–163（126 aa）
Chain C 38–163（126 aa）
Chain D 38–163（126 aa）
Not recorded NH2 AMINO GROUP × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；ammonium sulfate, sodium citrate-phosphate
Resolution 1.96 Å R-free 0.184
6SOS Engineered streptavidin variant (ENAGY) in complex with the Twin-Strep-tag peptide Deposited 2019-08-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 38–163（126 aa）
Chain B 38–163（126 aa）
Chain C 38–163（126 aa）
Chain D 38–163（126 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；ammonium sulfate, sodium acetate
Resolution 2.20 Å R-free 0.247
6T1E Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded GOL GLYCEROL × 12 ACT ACETATE ION × 4 HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；(NH4)2SO4 1.8 M, NaCH3COO 0.1 M, pH 4.6
Resolution 1.30 Å R-free 0.158
6T1G Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Not recorded HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Na-citrate 0.1 M pH 5.5, 40% PEG 600
Resolution 1.90 Å R-free 0.250
6T1K Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 EDO 1,2-ETHANEDIOL × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；CHES 0.1 M pH 9.5 30% PEG 3000
Resolution 1.20 Å R-free 0.171
6T2L Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-09 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；273 K；0.17M Na-acetate, 0.085M TRIS-HCl, pH 8.5, 25.5% PEG 4000, 15% glycerol
Resolution 1.00 Å R-free 0.134
6T2Y Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Not recorded EDO 1,2-ETHANEDIOL × 6 HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2M di-Sodium hydrogen phosphate 20% PEG 3350
Resolution 1.80 Å R-free 0.240
6T2Z Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Not recorded HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；1.6M Na2HPO4 0.4M K2HPO4 0.1M Sodium citrate phosphate pH 4.2
Resolution 1.35 Å R-free 0.183
6T30 Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Not recorded HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 EDO 1,2-ETHANEDIOL × 6 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2M di-Sodium hydrogen phosphate 20% PEG 3350
Resolution 1.80 Å R-free 0.230
6T31 Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Not recorded HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；CHES 0.1 M pH 9.5, 30% PEG 3000
Resolution 1.35 Å R-free 0.165
6T32 Streptavidin variants harbouring an artificial organocatalyst based cofactor Deposited 2019-10-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Not recorded HL9 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；CHES 0.1 M pH 9.5 30% PEG 3000
Resolution 1.75 Å R-free 0.191
6TIP Engineered streptavidin variant (YNAFM) in complex with the Strep-tag II peptide Deposited 2019-11-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 38–163（126 aa）
Not recorded NH2 AMINO GROUP × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；ammonium sulfate, sodium acetate
Resolution 2.10 Å R-free 0.228
6UC3 Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions Deposited 2019-09-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) BTN BIOTIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 3.5；298 K；500 ul of 0.1 M Citric Acid, pH 3.5, 3M NaCl in reservoir with 2 ul of reservoir buffer mixed with 2 ul of 10 mg/ml of protein in the drop. Crystals were soaked to pH 5.5 overnight with 3 exchanges
Resolution 1.84 Å R-free 0.213
6UD1 Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions Deposited 2019-09-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 3.5；298 K；500 ul of 0.1 M Citric Acid, pH 3.5, 3M NaCl in the reservoir with 2 ul of reservoir buffer mixed with 2 ul of 10 mg/ml of protein in the drop. Crystals were soaked to pH 5.5 overnight with 3 exchanges
Resolution 1.55 Å R-free 0.192
6UD6 Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions Deposited 2019-09-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 3.5；298 K；500 ul of 0.1 M citric acid, pH 3.5, 3M NaCl in the reservoir with 2 ul of reservoir buffer mixed with 2 ul of 10 mg/ml of protein in the drop. Crystals were soaked to pH 5.5 overnight with 3 exchanges
Resolution 1.50 Å R-free 0.190
6UD6 Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions Deposited 2019-09-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 14 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 3.5；298 K；500 ul of 0.1 M citric acid, pH 3.5, 3M NaCl in the reservoir with 2 ul of reservoir buffer mixed with 2 ul of 10 mg/ml of protein in the drop. Crystals were soaked to pH 5.5 overnight with 3 exchanges
Resolution 1.50 Å R-free 0.190
6UDB Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions Deposited 2019-09-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) PEG DI(HYDROXYETHYL)ETHER × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；500 ul of 0.1 M Bis-Tris, pH 6.5, 25% w/v polyethylene glycol 3350 in the reservoir with 2 ul of reservoir buffer mixed with 2 ul of 10 mg/ml of protein in the drop
Resolution 1.55 Å R-free 0.201
6UDC Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions Deposited 2019-09-19 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 37–163（127 aa）
Chain B 37–163（127 aa）
Chain C 37–163（127 aa）
Chain D 37–163（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；500 ul of 0.1 M Bis-Tris, pH 6.5, 25% w/v polyethylene glycol 3350 in the reservoir with 2 ul of reservoir buffer mixed with 2 ul of 10 mg/ml of protein in the drop
Resolution 2.10 Å R-free 0.256
6UI0 Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases Deposited 2019-09-29 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：K121A, E101Q, S112E QFY {N-(2-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 ACT ACETATE ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL protein, 2.0 Ammonium Sulfate, 0.1 M sodium acetate pH 4
Resolution 1.40 Å R-free 0.179
6UIU Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases Deposited 2019-10-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：K121A, E101Q, S112E QG7 N-(2-{bis[(pyridin-2-yl)methyl]amino}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL protein, 2.0 M ammonium sulfate, 0.1 M sodium acetate pH 4
Resolution 1.35 Å R-free 0.230
6UIY Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases Deposited 2019-10-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112E, K121A QG1 {5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-(2-{[(pyridin-2-yl)methyl][(pyridin-2-yl-kappaN)methyl]amino-kappaN}ethyl)pentanamide}iron(2+) × 4 ACT ACETATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL protein, 2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4
Resolution 1.47 Å R-free 0.177
6UIZ Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases Deposited 2019-10-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112E, K121A QG4 {N-(2-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}(triaza-1,2-dien-2-ium-1-ide-kappaN~1~)iron(4+) × 4 ACT ACETATE ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL, 2.0 M ammonium sulfate, 0.1 M sodium acetate pH 4
Resolution 1.85 Å R-free 0.234
6US6 Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases Deposited 2019-10-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：K121A, E101Q, S112E QFY {N-(2-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 ACT ACETATE ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL protein, 2.0 M ammonium sulfate, 0.1 M sodium acetate pH 4
Resolution 1.50 Å R-free 0.182
6VJK Streptavidin mutant M88 (N49C/A86C) Deposited 2020-01-16 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–160（123 aa）
Chain B 38–160（123 aa）
Chain E 38–160（123 aa）
Chain F 38–160（123 aa）
Mutation：N49C, A86C Mutation：N49C, A86C Mutation：N49C, A86C Mutation：N49C, A86C BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；21% PEG 3350, 8% glycerol, 100 mM Bis-Tris-Cl pH 7.5
Resolution 1.60 Å R-free 0.215
6VJK Streptavidin mutant M88 (N49C/A86C) Deposited 2020-01-16 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 38–160（123 aa）
Chain D 38–160（123 aa）
Chain I 38–160（123 aa）
Chain J 38–160（123 aa）
Mutation：N49C, A86C Mutation：N49C, A86C Mutation：N49C, A86C Mutation：N49C, A86C BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；21% PEG 3350, 8% glycerol, 100 mM Bis-Tris-Cl pH 7.5
Resolution 1.60 Å R-free 0.215
6VJK Streptavidin mutant M88 (N49C/A86C) Deposited 2020-01-16 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain G 38–160（123 aa）
Chain H 38–160（123 aa）
Chain K 38–160（123 aa）
Chain L 38–160（123 aa）
Mutation：N49C, A86C Mutation：N49C, A86C Mutation：N49C, A86C Mutation：N49C, A86C BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；21% PEG 3350, 8% glycerol, 100 mM Bis-Tris-Cl pH 7.5
Resolution 1.60 Å R-free 0.215
6VJL Streptavidin mutant M112 (G26C/A46C) Deposited 2020-01-16 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–183（159 aa）
Mutation：G26C, A46C BTN BIOTIN × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；294.15 K；28% PEG 4000, 0.15 M ammonium sulfate, 50 mM Bis-Tris
Resolution 1.30 Å R-free 0.201
6VO9 Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-01-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：K121A, L124Y KM3 {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 ACT ACETATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M ammonium sulfate, 0.1 M sodium acetate
Resolution 1.50 Å R-free 0.211
6VOB Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-01-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：K121A, L124Y KM3 {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 AZI AZIDE ION × 4 ACT ACETATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M ammonium sulfate, 0.1 M sodium acetate
Resolution 1.70 Å R-free 0.197
6VOZ Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-02-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：K121A, L124Y KM3 {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 ACT ACETATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M ammonium sulfate, 0.1 M sodium acetate
Resolution 1.30 Å R-free 0.206
6VP1 Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-02-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Mutation：K121A, L124Y Mutation：K121A, L124Y Mutation：K121A, L124Y Mutation：K121A, L124Y KM3 {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 ACT ACETATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M ammonium sulfate, 0.1 M sodium acetate
Resolution 1.45 Å R-free 0.197
6VP2 Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-02-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Mutation：K121A, L124Y Mutation：K121A, L124Y Mutation：K121A, L124Y Mutation：K121A, L124Y KM3 {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 AZI AZIDE ION × 2 ACT ACETATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M ammonium sulfate, 0.1 M sodium acetate
Resolution 1.80 Å R-free 0.199
6VP3 Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-02-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Mutation：K121A, L124Y Mutation：K121A, L124Y Mutation：K121A, L124Y Mutation：K121A, L124Y KM3 {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 ACT ACETATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M ammonium sulfate, 0.1 M sodium acetate
Resolution 1.65 Å R-free 0.221
6Y25 Streptavidin mutant S112R,K121E with a biotC4-1 cofactor - an artificial iron hydroxylase Deposited 2020-02-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 39–183（145 aa）
Mutation：S112R, K121E O6T biotC4-1 cofactor × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4
Resolution 1.95 Å R-free 0.203
6Y2M Streptavidin mutant S112R with a biotC4-1 cofactor - an artificial iron hydroxylase Deposited 2020-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 39–183（145 aa）
Not recorded O6T biotC4-1 cofactor × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4
Resolution 1.95 Å R-free 0.231
6Y2T Streptavidin wildtype with a biotC4-1 cofactor - an artificial iron hydroxylase Deposited 2020-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 39–183（145 aa）
Not recorded O6T biotC4-1 cofactor × 8 GOL GLYCEROL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4
Resolution 1.55 Å R-free 0.200
6Y33 Streptavidin mutant S112R with a biotC5-1 cofactor - an artificial iron hydroxylase Deposited 2020-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 39–183（145 aa）
Not recorded O7Q biotC5-1 cofactor × 4 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4
Resolution 1.49 Å R-free 0.192
6Y34 Streptavidin wildtype with a biotC5-1 cofactor - an artificial iron hydroxylase Deposited 2020-02-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 39–183（145 aa）
Not recorded O7Q biotC5-1 cofactor × 4 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4
Resolution 1.31 Å R-free 0.198
6Y3Q Streptavidin mutant S112R_K121E with a biotC5-1 cofactor - an artificial iron hydroxylase Deposited 2020-02-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 39–183（145 aa）
Not recorded O7Q biotC5-1 cofactor × 4 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4
Resolution 1.95 Å R-free 0.258
7ALX Sav-SOD: Chimeric Streptavidin-cSOD as Host for Artificial Metalloenzymes Deposited 2020-10-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 39–72（34 aa）
Chain AAA 73–183（111 aa）
Chain BBB 39–72（34 aa）
Chain BBB 73–183（111 aa）
Not recorded UFW ((4S)-1,3-dimesityl-4-((5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)methyl)imidazolidin-2-yl)gold × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；20 %w/v PEG 3350 (Precipitant) 0.2 M Na K Phos (Salt) 0.1 M BIS-TRIS prop 7.5 pH (Buffer)
Resolution 1.80 Å R-free 0.218
7B74 Chimeric Streptavidin With A Dimerization Domain For Artificial Transfer Hydrogenation Deposited 2020-12-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 39–72（34 aa）
Chain AAA 73–183（111 aa）
Chain BBB 39–72（34 aa）
Chain BBB 73–183（111 aa）
Chain CCC 39–72（34 aa）
Chain CCC 73–183（111 aa）
Chain DDD 39–72（34 aa）
Chain DDD 73–183（111 aa）
Not recorded 4IR {N-(4-{[2-(amino-kappaN)ethyl]sulfamoyl-kappaN}phenyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}(chloro)[(1,2,3,4,5-eta)-1,2,3,4,5-pentamethylcyclopentadienyl]iridium(III) × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；20 %w/v PEG 3350 (Polymer) 0.2 M KF (Salt)
Resolution 1.85 Å R-free 0.218
7DY0 1.93 A cryo-EM structure of streptavidin Deposited 2021-01-20 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–183（183 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.93 Å
7DY0 1.93 A cryo-EM structure of streptavidin Deposited 2021-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–183（183 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.93 Å
7DY0 1.93 A cryo-EM structure of streptavidin Deposited 2021-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–183（183 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.93 Å
7EFC 1.70 A cryo-EM structure of streptavidin Deposited 2021-03-21 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–183（183 aa）
Not recorded BTN BIOTIN × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.70 Å
7EFC 1.70 A cryo-EM structure of streptavidin Deposited 2021-03-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–183（183 aa）
Not recorded BTN BIOTIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.70 Å
7EFC 1.70 A cryo-EM structure of streptavidin Deposited 2021-03-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–183（183 aa）
Not recorded BTN BIOTIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.70 Å
7EFD 1.77 A cryo-EM structure of Streptavidin using first 40 frames (corresponding to about 40 e/A^2 total dose) Deposited 2021-03-21 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–183（183 aa）
Not recorded BTN BIOTIN × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.77 Å
7EFD 1.77 A cryo-EM structure of Streptavidin using first 40 frames (corresponding to about 40 e/A^2 total dose) Deposited 2021-03-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–183（183 aa）
Not recorded BTN BIOTIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.77 Å
7EFD 1.77 A cryo-EM structure of Streptavidin using first 40 frames (corresponding to about 40 e/A^2 total dose) Deposited 2021-03-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–183（183 aa）
Not recorded BTN BIOTIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.77 Å
7EK8 Crystal structure of apo streptavidin at ambient temperature Deposited 2021-04-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–160（123 aa）
Chain B 38–160（123 aa）
Chain C 38–160（123 aa）
Chain D 38–160（123 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；Pact PremierTM 100 mM MMT buffer pH 6.0 and 25 % w/v PEG 1500
Resolution 1.70 Å R-free 0.224
7EK9 Crystal structure of apo streptavidin at cryogenic temperature Deposited 2021-04-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–160（123 aa）
Chain B 38–160（123 aa）
Chain C 38–160（123 aa）
Chain D 38–160（123 aa）
Not recorded MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；294 K；Pact PremierTM 100 mM MMT buffer, PEG 1500
Resolution 1.10 Å R-free 0.187
7KBY Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-10-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：K121A, L124Y KM3 {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 CYN CYANIDE ION × 4 ACT ACETATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL 2.0 M ammonium sulfate, 0.1 M sodium acetate pH 4
Resolution 1.70 Å R-free 0.224
7KBZ Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-10-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Mutation：K121A, L124Y Mutation：K121A, L124Y Mutation：K121A, L124Y Mutation：K121A, L124Y KM3 {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+) × 4 CYN CYANIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；26 mg/mL 2.0 M ammonium sulfate, 0.1 M sodium acetate pH 4
Resolution 1.90 Å R-free 0.245
7KNL Artificial Metalloproteins with Dinuclear Iron Centers Deposited 2020-11-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：K121A, L124Y No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M Ammonium Sulfate, 0.1 M Sodium Acetate pH 4
Resolution 1.35 Å R-free 0.241
7NLV WILDTYPE CORE-STREPTAVIDIN WITH a conjugated BIOTINYLATED PYRROLIDINE II Deposited 2021-02-22 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain AAA 37–163（127 aa）
Chain BBB 37–163（127 aa）
Chain CCC 37–163（127 aa）
Chain DDD 37–163（127 aa）
Not recorded UJE 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-((S)-pyrrolidin-3-yl)pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；25 mg/mL protein in deionised water is mixed at equal volume with 52% MPD.
Resolution 1.29 Å R-free 0.247
7ZOF Streptavidin Iron-Porphyrin Deposited 2022-04-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Not recorded JLL (2R)-2-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-3-[[(5Z,10Z,14Z,19Z)-15-[[[(2R)-2-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-3-sulfo-propanoyl]amino]methyl]-1,4,21,23-tetrahydroporphyrin-5-yl]methylamino]-3-oxidanylidene-propane-1-sulfonic acid × 2 IMD IMIDAZOLE × 4 FE FE (III) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M MIB 7 pH (Buffer) 22.5 %v/v PEG 1500 (Precipitant)
Resolution 1.74 Å R-free 0.203
7ZX9 Streptavidin with a fluorescent substrate Deposited 2022-05-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Not recorded K9D 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-[6-(3,4-dihydro-1~{H}-isoquinolin-2-yl)-1,3-bis(oxidanylidene)benzo[de]isoquinolin-2-yl]ethyl]pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M ammonium sulfate, 0.1 M sodium acetate 26 mg/ml protein
Resolution 1.55 Å R-free 0.199
7ZXZ dithiol-ligand bound to streptavidin Deposited 2022-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 2 K9R 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[(1~{R},3~{S})-3-[3,5-bis(sulfanylmethyl)phenyl]-2,4-bis(oxidanylidene)cyclopentyl]pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；0.1 M MIB, 25 % w/v PEG 1500
Resolution 1.45 Å R-free 0.204
8AQD Hydrophobic probe bound to Streptavidin - 1 Deposited 2022-08-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded N9O 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-[6-(dimethylamino)-1,3-bis(oxidanylidene)benzo[de]isoquinolin-2-yl]ethyl]pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；25 w/v% PEG 3350 0.1 M sodium acetate pH 4.0
Resolution 1.45 Å R-free 0.240
8AQJ Hydrophobic probe bound to Streptavidin - 2 Deposited 2022-08-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded N9O 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-[6-(dimethylamino)-1,3-bis(oxidanylidene)benzo[de]isoquinolin-2-yl]ethyl]pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；25 % w/v PEG 3350 0.1 M sodium acetate pH 4.0
Resolution 1.85 Å R-free 0.234
8AQO Streptavidin with a bisbiothinilated Fe4S4 cluster Deposited 2022-08-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Not recorded NUI 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-[[20-[2-[5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethanoylamino]-2$l^{3},4,12,14$l^{3},16,24,25$l^{3},27$l^{3}-octathia-1$l^{4},3$l^{4},13$l^{4},15$l^{4}-tetraferranonacyclo[11.11.1.1^{1,13}.1^{6,10}.1^{18,22}.0^{2,15}.0^{3,14}.0^{3,25}.0^{15,27}]octacosa-6(28),7,9,18,20,22(26)-hexaen-8-yl]amino]-2-oxidanylidene-ethyl]pentanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；25 % w/v PEG 1500 0.1 M boric acid pH 8.5 (anaerobic)
Resolution 1.90 Å R-free 0.217
8AQX streptavidin mutant S112I with an iridium catalyst for CH activation Deposited 2022-08-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Not recorded NOF tert-butyl 7'-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-1-chloranyl-2,3,4,5,6-pentamethyl-spiro[1$l^{8}-iridapentacyclo[2.2.0.0^{1,3}.0^{1,5}.0^{2,6}]hexane-1,2'-3-aza-1-azonia-2$l^{8}-iridatricyclo[6.3.1.0^{4,12}]dodeca-1(11),4,6,8(12),9-pentaene]-3'-carboxylate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.2 M NaCl 0.1 M BIS-TRIS 6.5 pH 25 % w/v PEG 3350
Resolution 1.85 Å R-free 0.211
8AQY streptavidin mutant S112A with an iridium catalyst for CH activation Deposited 2022-08-15 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Not recorded NOF tert-butyl 7'-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-1-chloranyl-2,3,4,5,6-pentamethyl-spiro[1$l^{8}-iridapentacyclo[2.2.0.0^{1,3}.0^{1,5}.0^{2,6}]hexane-1,2'-3-aza-1-azonia-2$l^{8}-iridatricyclo[6.3.1.0^{4,12}]dodeca-1(11),4,6,8(12),9-pentaene]-3'-carboxylate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES 7.5 pH 10 % w/v PEG 8K 8 % v/v EG
Resolution 1.65 Å R-free 0.201
8BY0 streptavidin mutant S112I K121R with an iridium catalyst for CH activation Deposited 2022-12-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Not recorded NOF tert-butyl 7'-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-1-chloranyl-2,3,4,5,6-pentamethyl-spiro[1$l^{8}-iridapentacyclo[2.2.0.0^{1,3}.0^{1,5}.0^{2,6}]hexane-1,2'-3-aza-1-azonia-2$l^{8}-iridatricyclo[6.3.1.0^{4,12}]dodeca-1(11),4,6,8(12),9-pentaene]-3'-carboxylate × 4 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2.0 M ammonium sulfate 0.1 M sodium acetate (soaking under pH 6.0)
Resolution 2.10 Å R-free 0.251
8BY1 streptavidin with an iridium catalyst for CH activation Deposited 2022-12-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–158（120 aa）
Not recorded NOF tert-butyl 7'-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-1-chloranyl-2,3,4,5,6-pentamethyl-spiro[1$l^{8}-iridapentacyclo[2.2.0.0^{1,3}.0^{1,5}.0^{2,6}]hexane-1,2'-3-aza-1-azonia-2$l^{8}-iridatricyclo[6.3.1.0^{4,12}]dodeca-1(11),4,6,8(12),9-pentaene]-3'-carboxylate × 4 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；2 M ammonium sulfate 0.1 M sodium acetate (soaking at pH 6.0)
Resolution 1.49 Å R-free 0.195
8CRN Streptavidin S112Y Co-TAML artificial metalloenzyme Deposited 2023-03-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Chain C 39–183（145 aa）
Chain D 39–183（145 aa）
Not recorded VJL Co-linked Tetra-amido macrocyclic ligand × 4 NA SODIUM ION × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.1 M sodium acetate pH 4.0 2 M ammonium sulfate soaking with pH change to pH 6.0
Resolution 2.00 Å R-free 0.208
8CRP Streptavidin WT Co-TAML artificial metalloenzyme Deposited 2023-03-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Chain C 39–183（145 aa）
Chain D 39–183（145 aa）
Not recorded VJL Co-linked Tetra-amido macrocyclic ligand × 4 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；2 M ammonium sulfate, 0.1 M sodium acetate pH 4.0 for soaking pH adjustment to pH 6.0
Resolution 2.00 Å R-free 0.214
8GOG Structure of streptavidin mutant (S112Y-K121E) complexed with biotin-cyclopentadienyl-rhodium (III)(Cp*-Rh(III)) Deposited 2022-08-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–157（119 aa）
Chain B 39–157（119 aa）
Mutation：S112Y,K121E Mutation：S112Y,K121E RH3 RHODIUM(III) ION × 8 JSU trichloro((3~{a}~{S},4~{S},6~{a}~{R})-4-[(5~{R})-5-oxidanyl-5-[2-(2,3,4,5-tetramethylcyclopenta-2,4-dien-1-ylidene)ethylamino]pentyl]-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-2-one)rhodium(3+) × 4 GOL GLYCEROL × 18 CL CHLORIDE ION × 8 SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；291 K；0.2 M sodium sulfate pH 6.7 with 20% w/v PEG 3350
Resolution 2.00 Å R-free 0.294
8GVK Cryo-EM structure of streptavidin Deposited 2022-09-15 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–183（183 aa）
Chain B 1–183（183 aa）
Chain C 1–183（183 aa）
Chain D 1–183（183 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
8HRM Cryo-EM structure of streptavidin Deposited 2022-12-15 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 40–158（119 aa）
Chain B 40–158（119 aa）
Chain C 40–158（119 aa）
Chain D 40–158（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.56 Å
8OJW Streptavidin WT artificial metalloenzyme for carboamination Deposited 2023-03-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–159（122 aa）
Not recorded 0OD trichloro{(1,2,3,4,5-eta)-1,2,3,4-tetramethyl-5-[2-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)ethyl]cyclopentadienyl}rhodium(1+) × 4 CL CHLORIDE ION × 4 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2.0 M ammonium sulfate 0.1 M sodium acetate -> soaking experiment by pH adjustment to pH 6.0
Resolution 1.48 Å R-free 0.202
8OJX Streptavidin S112YK121E artificial metalloenzyme for carboamination Deposited 2023-03-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–158（121 aa）
Not recorded 0OD trichloro{(1,2,3,4,5-eta)-1,2,3,4-tetramethyl-5-[2-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)ethyl]cyclopentadienyl}rhodium(1+) × 4 IR IRIDIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；2 M ammonium sulfate 0.1 M sodium acetate pH 4.0 -> for soaking pH adjustment to pH 6.0
Resolution 1.60 Å R-free 0.197
8P5Y Artificial transfer hydrogenase with a Mn-12 cofactor and Streptavidin S112Y-K121M mutant Deposited 2023-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Chain C 39–183（145 aa）
Chain D 39–183（145 aa）
Mutation：S112Y-K121M Mutation：S112Y-K121M Mutation：S112Y-K121M Mutation：S112Y-K121M WZQ 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-(3,4-dihydro-2~{H}-pyrano[2,3-c]pyridin-6-ylmethylamino)ethyl]pentanamide × 4 MN MANGANESE (II) ION × 4 BR BROMIDE ION × 4 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；0.1 M Sodium/potassium phosphate, 0.1 M HEPES pH 7.5, 15 % v/v PEG, Smear High, 10 % v/v Ethylene glycol
Resolution 1.88 Å R-free 0.265
8P5Z Artificial transfer hydrogenase with a Mn-5 cofactor and Streptavidin S112Y-K121M mutant Deposited 2023-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Mutation：S112Y-K121M Mutation：S112Y-K121M Mutation：S112Y-K121M Mutation：S112Y-K121M X08 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-[(5-methylpyridin-2-yl)methylamino]ethyl]pentanamide × 4 MN MANGANESE (II) ION × 4 BR BROMIDE ION × 4 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.8；291 K；0.15 M Lithium sulfate, 0.05 M Magnesium chloride hexahydrate, 0.1 M Bis-Tris pH = 6.8, 25 % v/v PEG Smear Low
Resolution 1.56 Å R-free 0.218
8PXG Structure of Streptactin, solved at wavelength 2.75 A Deposited 2023-07-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–163（126 aa）
Not recorded CL CHLORIDE ION × 28 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；3 M sodium chloride, 0.1 citric acid, pH 3.5
Resolution 1.80 Å R-free 0.194
8QEX Streptavidin variant with a cobalt catalyst for CH metal-catalyzed hydrogen-atom-transfer (M-HAT) Deposited 2023-09-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–71（33 aa）
Chain A 78–183（106 aa）
Chain B 39–71（33 aa）
Chain B 78–183（106 aa）
Not recorded UFU cobalt Streptavidin × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；0.05 M Citric acid, 19 % w/v PEG 1000, 0.1 M Lithium sulfate, 0.05 M Sodium phosphate dibasic dihydrate
Resolution 1.90 Å R-free 0.254
8QQ3 Streptavidin with a Ni-cofactor Deposited 2023-10-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Chain C 39–183（145 aa）
Chain D 39–183（145 aa）
Not recorded WKF 4-[4-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]butylamino]-~{N}1,~{N}1'-di(quinolin-8-yl)cyclohexane-1,1-dicarboxamide × 4 NI NICKEL (II) ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M sodium acetate, 0.1 M Tris, pH 8.5, 30% w/v PEG 4K
Resolution 1.60 Å R-free 0.191
8TY0 Streptavidin variant S112E-K121H bound to bis-biotinylated Iron-porphyrin Deposited 2023-08-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Chain B 38–183（146 aa）
Chain C 38–183（146 aa）
Chain D 38–183（146 aa）
Not recorded SIK Bis-biotinylated Iron-porphyrin × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 , 20% PEG 4000
Resolution 1.54 Å R-free 0.191
8XG4 X-ray crystal structure of streptavidin flash-cooled in 30% glycerol at ambient pressure Deposited 2023-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 39–159（121 aa） Fragment：UNP residues 39-159
Chain D 39–159（121 aa） Fragment：UNP residues 39-159
Not recorded PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1 GOL GLYCEROL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG1,000
Resolution 1.38 Å R-free 0.169
8XG4 X-ray crystal structure of streptavidin flash-cooled in 30% glycerol at ambient pressure Deposited 2023-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 39–159（121 aa） Fragment：UNP residues 39-159
Chain C 39–159（121 aa） Fragment：UNP residues 39-159
Not recorded PG4 TETRAETHYLENE GLYCOL × 1 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG1,000
Resolution 1.38 Å R-free 0.169
8XG5 X-ray crystal structure of streptavidin flash-cooled in 30% PEG1000 at ambient pressure Deposited 2023-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 39–159（121 aa） Fragment：Avidin-like
Chain D 39–159（121 aa） Fragment：Avidin-like
Not recorded PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.40 Å R-free 0.183
8XG5 X-ray crystal structure of streptavidin flash-cooled in 30% PEG1000 at ambient pressure Deposited 2023-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 39–159（121 aa） Fragment：Avidin-like
Chain C 39–159（121 aa） Fragment：Avidin-like
Not recorded PG4 TETRAETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.40 Å R-free 0.183
8XG6 X-ray crystal structure of Streptavidine without cryo-protectant using a high-pressure cryocooling method Deposited 2023-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 39–159（121 aa） Fragment：Avidin-like
Chain D 39–159（121 aa） Fragment：Avidin-like
Not recorded PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.31 Å R-free 0.182
8XG6 X-ray crystal structure of Streptavidine without cryo-protectant using a high-pressure cryocooling method Deposited 2023-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 39–159（121 aa） Fragment：Avidin-like
Chain C 39–159（121 aa） Fragment：Avidin-like
Not recorded PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.31 Å R-free 0.182
8Y23 X-ray crystal structure of ALiS4-Streptavidine complex without cryo-protectant using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Chain C 39–159（121 aa）
Chain D 39–159（121 aa）
Not recorded 6FX methyl 5-(4-oxidanylidene-5~{H}-furo[3,2-c]pyridin-2-yl)pyridine-3-carboxylate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.48 Å R-free 0.193
8Y24 X-ray crystal structure of ALiS4-Streptavidine complex with 10% glycerol using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Chain C 39–159（121 aa）
Chain D 39–159（121 aa）
Not recorded DMS DIMETHYL SULFOXIDE × 1 GOL GLYCEROL × 5 PEG DI(HYDROXYETHYL)ETHER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.45 Å R-free 0.184
8Y25 X-ray crystal structure of ALiS4-Streptavidine complex with 20% glycerol using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Chain C 39–159（121 aa）
Chain D 39–159（121 aa）
Not recorded GOL GLYCEROL × 6 DMS DIMETHYL SULFOXIDE × 1 PEG DI(HYDROXYETHYL)ETHER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.45 Å R-free 0.188
8Y26 X-ray crystal structure of ALiS5-Streptavidine complex without cryo-protectant using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa） Fragment：UNP residues 39-159
Chain B 39–159（121 aa） Fragment：UNP residues 39-159
Chain C 39–159（121 aa） Fragment：UNP residues 39-159
Chain D 39–159（121 aa） Fragment：UNP residues 39-159
Not recorded DMS DIMETHYL SULFOXIDE × 4 A1LXQ dimethyl 5-(4-oxidanylidene-5~{H}-furo[3,2-c]pyridin-2-yl)benzene-1,3-dicarboxylate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.23 Å R-free 0.176
8Y27 X-ray crystal structure of ALiS5-Streptavidine complex with 10% glycerol using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa） Fragment：UNP residues 39-159
Chain B 39–159（121 aa） Fragment：UNP residues 39-159
Chain C 39–159（121 aa） Fragment：UNP residues 39-159
Chain D 39–159（121 aa） Fragment：UNP residues 39-159
Not recorded DMS DIMETHYL SULFOXIDE × 1 GOL GLYCEROL × 3 A1LXQ dimethyl 5-(4-oxidanylidene-5~{H}-furo[3,2-c]pyridin-2-yl)benzene-1,3-dicarboxylate × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG1,000
Resolution 1.45 Å R-free 0.185
8Y28 X-ray crystal structure of ALiS5-Streptavidine complex with 20% glycerol using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Chain C 39–159（121 aa）
Chain D 39–159（121 aa）
Not recorded GOL GLYCEROL × 4 DMS DIMETHYL SULFOXIDE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.33 Å R-free 0.185
8Y29 X-ray crystal structure of ALiS2-Streptavidine complex without cryo-protectant using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Chain C 39–159（121 aa）
Chain D 39–159（121 aa）
Not recorded MT6 methyl 3-(4-oxo-4,5-dihydrofuro[3,2-c]pyridin-2-yl)benzoate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG1,000
Resolution 1.42 Å R-free 0.192
8Y2A X-ray crystal structure of ALiS2-Streptavidine complex with 10% glycerol using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Chain C 39–159（121 aa）
Chain D 39–159（121 aa）
Not recorded MT6 methyl 3-(4-oxo-4,5-dihydrofuro[3,2-c]pyridin-2-yl)benzoate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.34 Å R-free 0.184
8Y2B X-ray crystal structure of ALiS2-Streptavidine complex with 20% glycerol using a high-pressure cryocooling method Deposited 2024-01-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–159（121 aa）
Chain B 39–159（121 aa）
Chain C 39–159（121 aa）
Chain D 39–159（121 aa）
Not recorded MT6 methyl 3-(4-oxo-4,5-dihydrofuro[3,2-c]pyridin-2-yl)benzoate × 2 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES pH 6.0, 20% PEG 1000
Resolution 1.50 Å R-free 0.179
8ZR1 Cocrystallization of engineered streptavidin with A9 oligo DNA Deposited 2024-06-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–163（125 aa）
Chain B 39–163（125 aa）
Chain C 39–163（125 aa）
Chain D 39–163（125 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 8；298 K；10 mM Tris-HCl
Resolution 2.60 Å R-free 0.281
8ZR2 Cocrystallization of engineered streptavidin with C9 oligo DNA Deposited 2024-06-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–163（125 aa）
Chain B 39–163（125 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 8；298 K；20 mM Tris-HCl
Resolution 2.50 Å R-free 0.346
8ZR2 Cocrystallization of engineered streptavidin with C9 oligo DNA Deposited 2024-06-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain C 39–163（125 aa）
Chain D 39–163（125 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 8；298 K；20 mM Tris-HCl
Resolution 2.50 Å R-free 0.346
9CST Streptavidin-E101Q-K121A bound to Cu(II)-biotin-ethyl-dipicolylamine cofactor Deposited 2024-07-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, K121A QG7 N-(2-{bis[(pyridin-2-yl)methyl]amino}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；ammonium sulfate, sodium acetate
Resolution 1.13 Å R-free 0.160
9CSU Streptavidin-E101Q-S112Y-K121A bound to Cu(II)-biotin-ethyl-dipicolylamine cofactor Deposited 2024-07-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112Y, K121A ACY ACETIC ACID × 4 QG7 N-(2-{bis[(pyridin-2-yl)methyl]amino}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；ammonium sulfate, sodium acetate
Resolution 1.60 Å R-free 0.207
9CSV Streptavidin-E101Q-S112Y-K121A bound to Cu(II)-biotin-ethyl-dipicolylamine cofactor, oxidized by hydrogen peroxide Deposited 2024-07-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112Y, K121A QG7 N-(2-{bis[(pyridin-2-yl)methyl]amino}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；ammonium sulfate, sodium acetate
Resolution 1.60 Å R-free 0.234
9CSW Streptavidin-E101Q-S112A-K121Y bound to Cu(II)-biotin-ethyl-dipicolylamine cofactor Deposited 2024-07-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112A, K121Y QG7 N-(2-{bis[(pyridin-2-yl)methyl]amino}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 8 ACY ACETIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；ammonium sulfate, sodium acetate
Resolution 1.30 Å R-free 0.189
9E6Z Streptavidin-E101Q-S112F-K121A bound to Cu(II)-biotin-ethyl-dipicolylamine cofactor Deposited 2024-10-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E101Q, S112F, K121A QG7 N-(2-{bis[(pyridin-2-yl)methyl]amino}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 ACY ACETIC ACID × 4 CU COPPER (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；296 K；ammonium sulfate, sodium acetate
Resolution 1.70 Å R-free 0.219
9EC5 Streptavidin-S112C-L124F bound to Cu(II)dpea cofactor, containing S-nitrosocysteine modification Deposited 2024-11-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：E14Q, S112C, L124F Non-standard monomer：Yes (specific site not provided by mmCIF) A1BIA N-(3-{bis[2-(pyridin-2-yl)ethyl]amino}propyl)-5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 8 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；ammonium sulfate, sodium acetate
Resolution 1.40 Å R-free 0.228
9EDF Streptavidin-S112C-L124F bound to Cu(II)dpea cofactor Deposited 2024-11-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：S112C, L124F A1BIA N-(3-{bis[2-(pyridin-2-yl)ethyl]amino}propyl)-5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 12 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；ammonium sulfate, sodium acetate
Resolution 1.57 Å R-free 0.183
9EDG Streptavidin-WT bound to Cu(II)dpea cofactor Deposited 2024-11-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa） Fragment：UNP residues 33-183
Not recorded A1BIA N-(3-{bis[2-(pyridin-2-yl)ethyl]amino}propyl)-5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 ACT ACETATE ION × 4 CU COPPER (II) ION × 8 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；ammonium sulfate, sodium acetate
Resolution 1.75 Å R-free 0.197
9EDH Streptavidin-S112C-T114F bound to Cu(II)dpea cofactor Deposited 2024-11-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：S112C, T114F A1BIA N-(3-{bis[2-(pyridin-2-yl)ethyl]amino}propyl)-5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；ammonium sulfate, sodium acetate
Resolution 1.72 Å R-free 0.222
9EDI Streptavidin-S112C-L124A bound to Cu(II)dpea cofactor Deposited 2024-11-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：S112C, L124A A1BIA N-(3-{bis[2-(pyridin-2-yl)ethyl]amino}propyl)-5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 12 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；ammonium sulfate, sodium acetate
Resolution 1.50 Å R-free 0.202
9EDK Streptavidin-S112C-T114V bound to Cu(II)dpea cofactor Deposited 2024-11-17 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：S112C, T114V A1BIA N-(3-{bis[2-(pyridin-2-yl)ethyl]amino}propyl)-5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 CU COPPER (II) ION × 16 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；ammonium sulfate, sodium acetate
Resolution 1.45 Å R-free 0.190
9EDU Streptavidin-S112C bound to Cu(II)dpea cofactor Deposited 2024-11-18 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–183（146 aa）
Mutation：S112C A1BIA N-(3-{bis[2-(pyridin-2-yl)ethyl]amino}propyl)-5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 ACT ACETATE ION × 4 CU COPPER (II) ION × 12 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；ammonium sulfate, sodium acetate
Resolution 1.85 Å R-free 0.183
9FFJ Artificial metalloenzyme with a nickel-based 1,10-phenanthroline cofactor and streptavidin N49M-S112V mutant Deposited 2024-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Not recorded EDO 1,2-ETHANEDIOL × 8 A1ICD N-methyl-N-[(4,4,6,6-tetrahydroxy-4,6-dioxido-1,3,3a,5,6a-tetrahydrothien[3,4-d]imidazol-4-ium-2-yl)methyl]-5-(2,4,4-trihydroxy-2-keto-3,3a,5,6-tetrahydro-1H-thien[3,4-d]imidazol-4-ium-6-yl × 4 PEG DI(HYDROXYETHYL)ETHER × 8 SO4 SULFATE ION × 8 NH4 AMMONIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；11% w/v PEG 8000 0.1M MES 6.0 0.24M Ammonium sulfate
Resolution 1.27 Å R-free 0.197
9FNR Artificial metalloenzyme with a nickel-based 1,10-phenanthroline cofactor and streptavidin S112V mutant Deposited 2024-06-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Not recorded A1ICD N-methyl-N-[(4,4,6,6-tetrahydroxy-4,6-dioxido-1,3,3a,5,6a-tetrahydrothien[3,4-d]imidazol-4-ium-2-yl)methyl]-5-(2,4,4-trihydroxy-2-keto-3,3a,5,6-tetrahydro-1H-thien[3,4-d]imidazol-4-ium-6-yl × 4 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；1.6M Magnesium sulfate heptahydrate,0.1M HEPES 7.2, 6% v/v Glycerol
Resolution 1.64 Å R-free 0.206
9FOA Artificial photoenzyme with anthraquinone cofactor and wild type streptavidin Deposited 2024-06-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain D 39–183（145 aa）
Not recorded A1ID2 ~{N}-[4-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]butyl]-9,10-bis(oxidanylidene)anthracene-2-carboxamide × 4 GOL GLYCEROL × 8 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.2M Ammonium sulfate, 20% w/v PEG 3350
Resolution 1.36 Å R-free 0.182
9I4R N-terminal Oic streptag II in Sav E44V-S45T-V47R-D67A-K121R variant Deposited 2025-01-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 39–183（145 aa）
Chain C 39–183（145 aa）
Chain D 39–183（145 aa）
Chain E 39–183（145 aa）
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.2M Ammonium citrate dibasic, 20% w/v PEG 3350
Resolution 1.84 Å R-free 0.213
9MFZ Streptavidin-E101Q-K121A-L124E bound to Fe(III)-biotin-pentyl-dipicolylamine cofactor Deposited 2024-12-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 38–158（121 aa）
Chain B 38–158（121 aa）
Chain C 38–158（121 aa）
Chain D 38–158（121 aa）
Mutation：E101Q, K121A, L124E Mutation：E101Q, K121A, L124E Mutation：E101Q, K121A, L124E Mutation：E101Q, K121A, L124E A1BLH N-(5-{bis[(pyridin-2-yl)methyl]amino}pentyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide × 4 FE FE (III) ION × 8 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；ammonium sulfate, sodium acetate
Resolution 1.55 Å R-free 0.189
9PUA L-Biotin-streptavidin binding Deposited 2025-07-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain J 37–163（127 aa）
Chain L 37–163（127 aa）
Not recorded A1CK6 5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid × 4 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；1 micro-liter protein containing 3 mM Biotin in 10 mM Tris-HCl pH 7.0 mixed with 1 micro-liter 38% Ammonium Sulfate, 100 mM Na-Acetate pH 4.5, 200 mM NaCl at 21C (294 K)
Resolution 0.94 Å R-free 0.147
9PUB Biotin-streptavidin binding Deposited 2025-07-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain J 37–163（127 aa）
Chain K 37–163（127 aa）
Not recorded BTN BIOTIN × 4 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；1 micro-liter protein containing 3 mM Biotin in 10 mM Tris-HCl pH 7.0 mixed with 1 micro-liter 38% Ammonium Sulfate, 100 mM Na-Acetate pH 4.5, 200 mM NaCl at 21C (294 K)
Resolution 0.95 Å R-free 0.145
9QNE Streptavidin with a thiophenol cofactor as artificial hydrogen atom transferase Deposited 2025-03-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Not recorded A1I7Y 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(3-sulfanylphenyl)pentanamide × 4 EDO 1,2-ETHANEDIOL × 24 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；0.1 M MES pH 5.5, 25 % w/v PEG 4K, 0.15 M (NH4)2SO4
Resolution 1.84 Å R-free 0.202
9QNP Streptavidin K121W with a thiophenol cofactor as artificial hydrogen atom transferase Deposited 2025-03-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Chain C 39–183（145 aa）
Chain D 39–183（145 aa）
Not recorded A1I7Y 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(3-sulfanylphenyl)pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10 % w/v PEG 1K, 10 % w/v PEG 8K
Resolution 2.30 Å R-free 0.254
9QNZ Streptavidin 112Y-121W-124F with a thiophenol cofactor as artificial hydrogen atom transferase Deposited 2025-03-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Chain B 39–183（145 aa）
Chain C 39–183（145 aa）
Chain D 39–183（145 aa）
Not recorded A1I7Y 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(3-sulfanylphenyl)pentanamide × 4 ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；0.15 M NH4 acetate, 0.1 M BIS-TRIS pH 5.5, 45 % v/v MPD
Resolution 1.88 Å R-free 0.201
9QO7 Streptavidin K121M with a thiophenol cofactor as artificial hydrogen atom transferase Deposited 2025-03-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 39–183（145 aa）
Not recorded A1I8C ~{N}-[4-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]butyl]-3-sulfanyl-benzamide × 4 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；1 M Na3 citrate, 0.1 M Na cacodylate pH 6.5
Resolution 2.00 Å R-free 0.278

312 other PDB entries and 368 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SAV_STRAV
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–127； UniProt 37–163 Author chain B； PDBConstruct 1–127； UniProt 37–163 Author chain C； PDBConstruct 1–127； UniProt 37–163 Author chain D； PDBConstruct 1–127； UniProt 37–163

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1n9m

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1n9m
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1n9m
Deposition date deposition_date2002-11-25
Structure title titleStreptavidin Mutant S27A with Biotin at 1.6A Resolution
Keywords keywordshomotetramer, BIOTIN-BINDING PROTEIN; BIOTIN-BINDING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier23.13 Å
Radius of gyration Rg (electron density) rg_electron21.99 Å
Forward intensity I(0) i048700900.00
Molecular weight molecular_weight52214.0 kDa
Excluded volume excluded_volume64383 ų
Envelope volume envelope_volume77760 ų
Hydration-shell volume shell_volume28239 ų
Envelope diameter envelope_diameter72.7 Å
Shell Rg shell_rg29.76 Å
Envelope Rg envelope_rg22.28 Å
Shape Rg shape_rg21.96 Å
Total Rg total_rg22.94 Å
Total atoms total_atoms3696
Residues n_residues488
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax68.2 Å
Rg (real space) rg_real22.96 Å
Rg uncertainty (real space) rg_real_error0.34 Å
I(0) (real space) i0_real4.8700e+07
I(0) uncertainty (real space) i0_real_error5.8250e+05
Rg (reciprocal space) rg_reciprocal23.00 Å
I(0) (reciprocal space) i0_reciprocal48700000.0000
Solution quality estimate total_estimate0.8324
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary30.4 Å
Skewness Skewness skewness0.095
Kurtosis Kurtosis kurtosis-0.526
Angular range angular_range— – 0.3450 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha14430000.0000
Real-space data points n_real_points66
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.949; Stabil: 0.997; Sysdev: 1.000; Positv: 1.000; Valcen: 0.979; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

Domain ID domain_idd1n9ma_
Class classb — All beta proteins
Fold Fold foldb.61 — Streptavidin-like
Superfamily Superfamily superfamilyb.61.1 — Avidin/streptavidin
Family Family familyb.61.1.1 — Avidin/streptavidin
Domain ID domain_idd1n9mb_
Class classb — All beta proteins
Fold Fold foldb.61 — Streptavidin-like
Superfamily Superfamily superfamilyb.61.1 — Avidin/streptavidin
Family Family familyb.61.1.1 — Avidin/streptavidin
Domain ID domain_idd1n9mc_
Class classb — All beta proteins
Fold Fold foldb.61 — Streptavidin-like
Superfamily Superfamily superfamilyb.61.1 — Avidin/streptavidin
Family Family familyb.61.1.1 — Avidin/streptavidin
Domain ID domain_idd1n9md_
Class classb — All beta proteins
Fold Fold foldb.61 — Streptavidin-like
Superfamily Superfamily superfamilyb.61.1 — Avidin/streptavidin
Family Family familyb.61.1.1 — Avidin/streptavidin

CATH v4.4 (4 domains)

Domain ID domain_id1n9mA00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology128 — Lipocalin
Homologous superfamily homologous superfamily30 — Avidin-like
Domain ID domain_id1n9mB00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology128 — Lipocalin
Homologous superfamily homologous superfamily30 — Avidin-like
Domain ID domain_id1n9mC00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology128 — Lipocalin
Homologous superfamily homologous superfamily30 — Avidin-like
Domain ID domain_id1n9mD00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology128 — Lipocalin
Homologous superfamily homologous superfamily30 — Avidin-like
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8. Citations (1)

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9. Files and Curves (10)