2e1q

Crystal Structure of Human Xanthine Oxidoreductase mutant, Glu803Val

Method: X-RAY DIFFRACTION Dmax: 212.9 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 2e1q

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2e1q
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2e1q
沉积日期 deposition_date2006-10-27
结构标题 titleCrystal Structure of Human Xanthine Oxidoreductase mutant, Glu803Val
关键词 keywordsxanthine oxidase, molybdenum cofactor, FAD, OXIDOREDUCTASE; OXIDOREDUCTASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier69.72
回转半径 Rg (电子) rg_electron70.22
零角强度 I(0) i04773350000.00
分子量 molecular_weight582640.0 kDa
排除体积 excluded_volume728110 ų
包络体积 envelope_volume978150 ų
水化壳体积 shell_volume117850 ų
包络直径 envelope_diameter251.4
壳层 Rg shell_rg66.24
包络 Rg envelope_rg69.21
形状 Rg shape_rg70.24
总 Rg total_rg70.07
总原子数 total_atoms40772
残基数 n_residues5228
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax212.9
Rg (实空间) rg_real69.64
Rg 误差 (实空间) rg_real_error1.29
I(0) (实空间) i0_real4.7630e+09
I(0) 误差 (实空间) i0_real_error9.1880e+07
Rg (倒空间) rg_reciprocal68.59
I(0) (倒空间) i0_reciprocal4760000000.0000
解质量估计 total_estimate0.8305
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary48.4
偏度 Skewness skewness0.380
峰度 Kurtosis kurtosis-0.582
角度范围 angular_range— – 0.1100 −1
当前正则化参数 α current_alpha0.0234
最高正则化参数 α highest_alpha304700000.0000
实空间数据点数 n_real_points23
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.930; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.982; Smooth: 0.019

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (9)

6. 折叠分类 (SCOP + CATH) 20 domains

CATH v4.4 (20 domains)

结构域编号 domain_id2e1qC01
类 Class class3 — Alpha Beta
架构 Architecture architecture10 — Roll
拓扑 Topology topology20 — Ubiquitin-like (UB roll)
同源超家族 H-superfamily homologous superfamily30 — Beta-grasp domain
结构域编号 domain_id2e1qC02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology150 — DNA polymerase; domain 1
同源超家族 H-superfamily homologous superfamily120 — [2Fe-2S]-binding domain
结构域编号 domain_id2e1qC03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology43 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 2
同源超家族 H-superfamily homologous superfamily10 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2
结构域编号 domain_id2e1qC04
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology465 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2e1qC05
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology390 — Enolase-like; domain 1
同源超家族 H-superfamily homologous superfamily50 — CO dehydrogenase flavoprotein, C-terminal domain
结构域编号 domain_id2e1qC06
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology365 — Aldehyde Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain
结构域编号 domain_id2e1qC07
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1170 — Aldehyde Oxidoreductase; domain 3
同源超家族 H-superfamily homologous superfamily50 — Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead
结构域编号 domain_id2e1qC08
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology365 — Aldehyde Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain
结构域编号 domain_id2e1qC09
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology365 — Aldehyde Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain
结构域编号 domain_id2e1qC10
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology365 — Aldehyde Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain
结构域编号 domain_id2e1qD01
类 Class class3 — Alpha Beta
架构 Architecture architecture10 — Roll
拓扑 Topology topology20 — Ubiquitin-like (UB roll)
同源超家族 H-superfamily homologous superfamily30 — Beta-grasp domain
结构域编号 domain_id2e1qD02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology150 — DNA polymerase; domain 1
同源超家族 H-superfamily homologous superfamily120 — [2Fe-2S]-binding domain
结构域编号 domain_id2e1qD03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology43 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 2
同源超家族 H-superfamily homologous superfamily10 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2
结构域编号 domain_id2e1qD04
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology465 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2e1qD05
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology390 — Enolase-like; domain 1
同源超家族 H-superfamily homologous superfamily50 — CO dehydrogenase flavoprotein, C-terminal domain
结构域编号 domain_id2e1qD06
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology365 — Aldehyde Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain
结构域编号 domain_id2e1qD07
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1170 — Aldehyde Oxidoreductase; domain 3
同源超家族 H-superfamily homologous superfamily50 — Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead
结构域编号 domain_id2e1qD08
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology365 — Aldehyde Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain
结构域编号 domain_id2e1qD09
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology365 — Aldehyde Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain
结构域编号 domain_id2e1qD10
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology365 — Aldehyde Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain

7. 引用文献 (1)

8. 文件与曲线 (10)