2j20
Human p53 core domain mutant M133L-V203A-N239Y-N268D-R273C
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 2j20 |
| 沉积日期 deposition_date | 2006-08-15 |
| 结构标题 title | Human p53 core domain mutant M133L-V203A-N239Y-N268D-R273C |
| 关键词 keywords | ;SECOND-SITE SUPPRESSOR MUTATION, DISEASE MUTATION, NUCLEAR PROTEIN, PHOSPHORYLATION, TUMOR SUPPRESSOR, ALTERNATIVE SPLICING, LI-FRAUMENI SYNDROME, LI- FRAUMENI SYNDROME, HOST-VIRUS INTERACTION, TRANSCRIPTION, METAL-BINDING, ANTI-ONCOGENE, DNA-BINDING, TRANSFERASE, POLYMORPHISM, GLYCOPROTEIN, ZINC, ACTIVATOR, APOPTOSIS, CELL CYCLE, ACETYLATION, P53 DNA-BINDING DOMAIN, TRANSCRIPTION REGULATION, SUPERSTABLE MUTANT, DNA-BINDING PROTEIN ;; NUCLEAR PROTEIN |
| 实验方法 method | X-RAY DIFFRACTION |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 24.47 Å |
| 回转半径 Rg (电子) rg_electron | 23.46 Å |
| 零角强度 I(0) i0 | 37325300.00 |
| 分子量 molecular_weight | 44080.0 kDa |
| 排除体积 excluded_volume | 53858 ų |
| 包络体积 envelope_volume | 67621 ų |
| 水化壳体积 shell_volume | 24137 ų |
| 包络直径 envelope_diameter | 76.6 Å |
| 壳层 Rg shell_rg | 30.23 Å |
| 包络 Rg envelope_rg | 23.27 Å |
| 形状 Rg shape_rg | 23.45 Å |
| 总 Rg total_rg | 24.24 Å |
| 总原子数 total_atoms | 3068 |
| 残基数 n_residues | 389 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 75.0 Å |
| Rg (实空间) rg_real | 24.38 Å |
| Rg 误差 (实空间) rg_real_error | 0.41 Å |
| I(0) (实空间) i0_real | 3.7330e+07 |
| I(0) 误差 (实空间) i0_real_error | 4.5500e+05 |
| Rg (倒空间) rg_reciprocal | 24.40 Å |
| I(0) (倒空间) i0_reciprocal | 37330000.0000 |
| 解质量估计 total_estimate | 0.9157 |
| 解质量评级 solution_quality | EXCELLENT a EXCELLENT solution |
| P(r) 峰数 n_peaks | 2 |
| 主峰位置 r_peak_primary | 29.8 Å |
| 偏度 Skewness skewness | 0.180 |
| 峰度 Kurtosis kurtosis | -0.583 |
| 角度范围 angular_range | — – 0.3250 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 3966000.0000 |
| 实空间数据点数 n_real_points | 64 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.970; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.989 |
4. 晶体学与实验 4. Crystallography & Experiment
5. 实体与聚合体信息 (4)
6. 折叠分类 (SCOP + CATH) 4 domains
SCOP 2.08 (2 domains)
| 结构域编号 domain_id | d2j20a_ |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.2 — Common fold of diphtheria toxin/transcription factors/cytochrome f |
| 超家族 Superfamily superfamily | b.2.5 — p53-like transcription factors |
| 家族 Family family | b.2.5.2 — p53 DNA-binding domain-like |
| 结构域编号 domain_id | d2j20b_ |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.2 — Common fold of diphtheria toxin/transcription factors/cytochrome f |
| 超家族 Superfamily superfamily | b.2.5 — p53-like transcription factors |
| 家族 Family family | b.2.5.2 — p53 DNA-binding domain-like |
CATH v4.4 (2 domains)
| 结构域编号 domain_id | 2j20A00 |
| 类 Class class | 2 — Mainly Beta |
| 架构 Architecture architecture | 60 — Sandwich |
| 拓扑 Topology topology | 40 — Immunoglobulin-like |
| 同源超家族 H-superfamily homologous superfamily | 720 — |
| 结构域编号 domain_id | 2j20B00 |
| 类 Class class | 2 — Mainly Beta |
| 架构 Architecture architecture | 60 — Sandwich |
| 拓扑 Topology topology | 40 — Immunoglobulin-like |
| 同源超家族 H-superfamily homologous superfamily | 720 — |