8fne

phiPA3 PhuN Tetramer, p2

Method: ELECTRON MICROSCOPY D_max: 157.9 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8fne
沉积日期 deposition_date	2022-12-27
结构标题 title	phiPA3 PhuN Tetramer, p2
关键词 keywords	phiPA3 protein, shell protein, PhuN, phage nucleus, VIRAL PROTEIN; VIRAL PROTEIN
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	48.45 Å
回转半径 Rg (电子) rg_electron	48.18 Å
零角强度 I(0) i0	845359000.00
分子量 molecular_weight	239430.0 kDa
排除体积 excluded_volume	298670 Å³
包络体积 envelope_volume	424210 Å³
水化壳体积 shell_volume	70913 Å³
包络直径 envelope_diameter	157.7 Å
壳层 Rg shell_rg	54.40 Å
包络 Rg envelope_rg	47.39 Å
形状 Rg shape_rg	48.18 Å
总 Rg total_rg	48.41 Å
总原子数 total_atoms	33508
残基数 n_residues	2148
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	157.9 Å
Rg (实空间) rg_real	48.29 Å
Rg 误差 (实空间) rg_real_error	1.08 Å
I(0) (实空间) i0_real	8.4540e+08
I(0) 误差 (实空间) i0_real_error	1.3710e+07
Rg (倒空间) rg_reciprocal	48.45 Å
I(0) (倒空间) i0_reciprocal	845500000.0000
解质量估计 total_estimate	0.8925
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	71.8 Å
偏度 Skewness skewness	0.123
峰度 Kurtosis kurtosis	-0.668
角度范围 angular_range	— – 0.1650 Å⁻¹
当前正则化参数 α current_alpha	0.0001
最高正则化参数 α highest_alpha	174800000.0000
实空间数据点数 n_real_points	34
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.903; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.889

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (1)

Entity 1 polymer

描述 description	Maltose/maltodextrin-binding periplasmic protein, PhuN
分子量 formula_weight	110126.1 kDa
来源方法 src_method	man
拷贝数 entity_count	8

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H
原子数 atom_count	33508

HHHHHHMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTGKPIPNPLLGLDSTENLYFQGMQQTQQGPKVQTQTLQGGAGNLNSIFQRSGRTDGGDARASEALAVFNKLKEEAIAQQDLHDDFLVFRFDRDQNRVGYSALLVVKRAAINGQQVIVTRPLVMPNDQITLPTKKLTIQNGMHQETIEAEADVQDVFTTQYWNRICDSIRQQTGKHDAMVINAGPTVIPADFDLKDELVLKQLLIKSVNLCDDMLAKRSGEQPFSVAMLKGTDETLAARLNFTGKPMHDSLGYPIRSDILVSLNRVKKPGQQENEFYEAEDKLNQVSCFVNLEYTPQPQQAIYGAPQQTQQLPPLTPAIVITDVRQAEWLKANTMELYLFALSNAFRVTANQSWARSLLPQLGKVKDMRDIGAIGYLSRLAARVETKTETFTDQNFAELLYNMVRPSPVFMSDLNRFGDNAAIENVFIDALGGVNQQRAVAAIIAGVNNLIGGGFEKFFDHNTMPIIQPYGTDIQLGYYLDGEGEKQDRRDLDVLGALNASDGNIQEWMSWYGTQCNVAVHPELRARQSKNFDRQYLGNSVTYTTRAHRGIWNPKFIEALDKAIASVGLTVAMDNVAQVFGAQRFSGNLAIADYAVTGTAQVSSGLVSNGGYNPQFGVGQGSGFY

来源 Source

来源生物 organism	Pseudomonas phage PhiPA3
NCBI 分类编号 ncbi_taxonomy_id	998086
宿主生物 host_organism	Escherichia coli BL21(DE3)

▶

7. 引用文献 (1)

▶

分辨率 resolution	3.90 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	29303

fit	/app/data/saxs_computation/fn/8fne/8fne.fit
pddf	/app/data/pr_computation/fn/8fne/8fne.pr.out
profile	/app/data/saxs_computation/fn/8fne/8fne.dat