8fr8

Structure of Mycobacterium smegmatis Rsh bound to a 70S translation initiation complex

Method: ELECTRON MICROSCOPY D_max: 295.1 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8fr8
沉积日期 deposition_date	2023-01-06
结构标题 title	Structure of Mycobacterium smegmatis Rsh bound to a 70S translation initiation complex
关键词 keywords	starvation sensing, RelA/SpoT homologue, Mycobacterium, 70S initiation complex, TRANSLATION; TRANSLATION
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	83.75 Å
回转半径 Rg (电子) rg_electron	84.41 Å
零角强度 I(0) i0	167450000000.00
分子量 molecular_weight	2269800.0 kDa
排除体积 excluded_volume	2335400 Å³
包络体积 envelope_volume	3980000 Å³
水化壳体积 shell_volume	357530 Å³
包络直径 envelope_diameter	271.0 Å
壳层 Rg shell_rg	100.10 Å
包络 Rg envelope_rg	82.93 Å
形状 Rg shape_rg	84.47 Å
总 Rg total_rg	84.38 Å
总原子数 total_atoms	152819
残基数 n_residues	11140
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	295.1 Å
Rg (实空间) rg_real	85.70 Å
Rg 误差 (实空间) rg_real_error	1.66 Å
I(0) (实空间) i0_real	1.6620e+11
I(0) 误差 (实空间) i0_real_error	3.9310e+09
Rg (倒空间) rg_reciprocal	85.32 Å
I(0) (倒空间) i0_reciprocal	168200000000.0000
解质量估计 total_estimate	0.9057
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	108.7 Å
偏度 Skewness skewness	0.345
峰度 Kurtosis kurtosis	0.066
角度范围 angular_range	— – 0.0950 Å⁻¹
当前正则化参数 α current_alpha	1.0720
最高正则化参数 α highest_alpha	7909000000.0000
实空间数据点数 n_real_points	20
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.748; Stabil: 0.924; Sysdev: 1.000; Positv: 1.000; Valcen: 0.957; Smooth: 0.878

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (58)

Entity 1 polymer

描述 description	50S ribosomal protein L33 2
分子量 formula_weight	6484.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	c
原子数 atom_count	152820

ARNEIRPIVKLRSTAGTGYTYVTRKNRRNDPDRIVLRKYDPVLRRHVEFREER

Entity 2 polymer

描述 description	50S ribosomal protein L28
分子量 formula_weight	8730.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	y

SAHCQVTGRGPGFGNSVSHSHRRTPRRWDPNIQNKTYYLPSEGRRIRLRVSAKGIKVIDRDGIEAVVARMRARGEKV

Entity 3 polymer

描述 description	fMet tRNA (77-MER)
分子量 formula_weight	24780.7 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	E

CGCGGGGUGGAGCAGCUCGGUAGCUCGCUGGGCUCAUAACCCAGAGGUCGCAGGUUCGAAUCCUGUCCCCGCUACCA

来源 Source

来源生物 organism	Mycolicibacterium smegmatis MC2 155
NCBI 分类编号 ncbi_taxonomy_id	246196
宿主生物 host_organism	Escherichia coli

Entity 4 polymer

描述 description	30S ribosomal protein S19
分子量 formula_weight	9379.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	d

PRSLKKGPFVDDHLLKKVDVQNEKNTKQVIKTWSRRSTIIPDFIGHTFAVHDGRKHVPVFVTEAMVGHKLGEFAPTRTFKGH

Entity 5 polymer

描述 description	5S rRNA (118-MER)
分子量 formula_weight	38061.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	B

GUUACGGCGGUCCAUAGCGGCAGGGAAACGCCCGGUCCCAUCCCGAACCCGGAAGCUAAGCCUGCCAGCGCCGAUGAUACUACCCAUCCGGGUGGAAAAGUAGGACACCGCCGAACAC

Entity 6 polymer

描述 description	30S ribosomal protein S13
分子量 formula_weight	13374.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	j

ARLVGVDLPRDKRMEIALTYIYGIGRTRSNEILAATGIDKNMRTKDLTDDQVTVLRDYIEGNLKVEGDLRREVQADIRRKIEIGCYQGLRHRRGLPVRGQRTKTNARTRKGPKRTI

Entity 7 polymer

描述 description	50S ribosomal protein L16
分子量 formula_weight	15498.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

MLIPRKVKHRKQHHPEQRGIASGGTSVSFGDYGIQALEHAYITNRQIESARIAINRHIKRGGKVWINIFPDRPLTKKPAETRMGSGKGSPEWWVANVKPGRVLFELSYPDEKTARDALTRAIHKLPIKARIVTREE

Entity 8 polymer

描述 description	50S ribosomal protein eL31
分子量 formula_weight	2710.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3

AKRGRKKRDRKHSKANHGKRPNA

Entity 9 polymer

描述 description	23S rRNA (3119-MER)
分子量 formula_weight	1011834.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	A

AAGUGUUUAAGGGCGCAUGGUGGAUGCCUUGGCACUGGGAGCCGAUGAAGGACGUAGGAGGCUGCGAUAAGCCUCGGGGAGCUGUCAACCGAGCGUUGAUCCGAGGAUGUCCGAAUGGGGAAACCCGGCACGAGUGAUGUCGUGUCACCAGGCGCUGAAUAUAUAGGCGUCUGGGGGGAACGCGGGGAAGUGAAACAUCUCAGUACCCGUAGGAAGAGAAAACAAAAUGUGAUUCCGUGAGUAGUGGCGAGCGAAAGCGGAGGAUGGCUAAACCGUAUGCAUGUGAUACCGGGUAGGGGUUGUGUGUGCGGGGUUGUGGGACCUAUCUUUCCGGCUCUACCUGGCUGGAGGGCAGUGAGAAAAUGUUGUGGUUAGCGGAAAUGGCUUGGGAUGGCCUGCCGUAGACGGUGAGAGCCCGGUACGUGAAAACCCGACGUCUGUCUUGAUGGUGUUCCCGAGUAGCAGCGGGCCCGUGGAAUCUGCUGUGAAUCUGCCGGGACCACCCGGUAAGCCUGAAUACUUCCCAGUGACCGAUAGCGGAUUAGUACCGUGAGGGAAUGGUGAAAAGUACCCCGGGAGGGGAGUGAAAGAGUACCUGAAACCGUGCGCUUACAAUCCGUCAGAGCCCUCGACGUGUCGUGGGGUGAUGGCGUGCCUUUUGAAGAAUGAGCCUGCGAGUCAGGGACAUGUCGCGAGGUUAACCCGGGUGGGGUAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGUAUCCACACAAGAGUGUGUGGUGUAGUGGUGUGUUCUGGACCCGAAGCGGAGUGAUCUACCCAUGGCCAGGGUGAAGCGCGGGUAAGACCGCGUGGAGGCCCGAACCCACUUAGGUUGAAGACUGAGGGGAUGAGCUGUGGGUAGGGGUGAAAGGCCAAUCAAACUCCGUGAUAGCUGGUUCUCCCCGAAAUGCAUUUAGGUGCAGCGUCGCAUGUUUCUUGCCGGAGGUAGAGCUACUGGAUGGCCGAUGGGCCCCACAGGGUUACUGACGUCAGCCAAACUCCGAAUGCCGGUAAGUCCAAGAGUGCGGCAGUGAGACGGCGGGGGAUAAGCUCCGUGCGUCGAGAGGGAAACAGCCCAGAUCGCCGGCUAAGGCCCCUAAGCGUGUGCUAAGUGGAAAAGGAUGUGCAGUCGCGAAGACAACCAGGAGGUUGGCUUAGAAGCAGCCACCCUUGAAAGAGUGCGUAAUAGCUCACUGGUCAAGUGAUUGUGCGCCGAUAAUGUAGCGGGGCUCAAGCACACCGCCGAAGCCGCGGCAGCCAACGUGUUGGCUGGGUAGGGGAGCGUCCUGCAUCCGGUGAAGCCGCCGAGUGAUCGAGUGGUGGAGGGUGUGGGAGUGAGAAUGCAGGCAUGAGUAGCGAUUAGGCAAGUGAGAACCUUGCCCGCCGAAAGACCAAGGGUUCCUGGGCCAGGCCAGUCCGCCCAGGGUGAGUCGGGACCUAAGGCGAGGCCGACAGGCGUAGUCGAUGGACAACGGGUUGAUAUUCCCGUACCCGUGUAUGUGCGUCCAUGAUGAAUCAGCGGUACUAACCAUCCAAAACCACCGUGACCGCACCUUUCGGGGUGUGGCGUUGGUGGGGCUGCAUGGGACCUUCGUUGGUAGUAGUCAAGCGAUGGGGUGACGCAGGAAGGUAGCCGUACCGGUCAGUGGUAAUACCGGGGUAAGCCUGUAGGGAGUCAGAUAGGUAAAUCCGUCUGGCAUAUAUCCUGAGAGGUGAUGCAUAGCCGAGUGAGGCGAAUUCGGUGAUCCUAUGCUGCCGAGAAAAGCCUCUAGCGAGGACAUACACGGCCCGUACCCCAAACCAACACAGGUGGUCAGGUAGAGAAUACUAAGGCGUACGAGUGAACUAUGGUUAAGGAACUCGGCAAAAUGCCCCCGUAACUUCGGGAGAAGGGGGACCCACAUGGCGUGUAAGCCUUUACGGCCCAAGCGUGAGUGGGUGGCACAAACCAGUGAGAAGCGACUGUUUACUAAAAACACAGGUCCGUGCGAAGUCGCAAGACGAUGUAUACGGACUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGACCCGUUAACUCCCUUUGGGGGUGAAGCGGAGAAUUUAAGCCCCAGUAAACGGCGGUGGUAACUAUAACCAUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAACGACUUCUCAACUGUCUCAACCAUAGACUCGGCGAAAUUGCACUACGAGUAAAGAUGCUCGUUACGCGCGGCAGGACGAAAAGACCCCGGGACCUUCACUACAACUUGGUAUUGGUGCUCGAUACGGUUUGUGUAGGAUAGGUGGGAGACUGUGAAGCUCACACGCCAGUGUGGGUGGAGUCGUUGUUGAAAUACCACUCUGAUCGUAUUGGGCCUCUAACCUCGGACCGUAUAUCCGGUUCAGGGACAGUGCCUGGUGGGUAGUUUAACUGGGGCGGUUGCCUCCUAAAAUGUAACGGAGGCGCCCAAAGGUUCCCUCAACCUGGACGGCAAUCAGGUGUUGAGUGUAAGUGCACAAGGGAGCUUGACUGCGAGACGGACAUGUCGAGCAGGGACGAAAGUCGGGACUAGUGAUCCGGCACCUCUGAGUGGAAGGGGUGUCGCUCAACGGAUAAAAGGUACCCCGGGGAUAACAGGCUGAUCUUCCCCAAGAGUCCAUAUCGACGGGAUGGUUUGGCACCUCGAUGUCGGCUCGUCGCAUCCUGGGGCUGGAGCAGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGCACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCUCUAUCCGCCGCGCGCGUCAGAAGCUUGAGGAAACCUGUCCCUAGUACGAGAGGACCGGGACGGACGAACCUCUGGUAUACCAGUUGUCCCACCAGGGGCACGGCUGGAUAGCCACGUUCGGACAGGAUAACCGCUGAAAGCAUCUAAGCGGGAAACCUCUUCCAAGACCAGGCUUCUCACCCUCUAGGAGGGAUAAGGCCCCCCGCAGACCACGGGAUUGAUAGACCAGACCUGGAAGCCUAGUAAUAGGUGCAGGGAACUGGCACUAACCGGCCGAAAACUUAC

Entity 10 polymer

描述 description	50S ribosomal protein L2
分子量 formula_weight	30008.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K

GIRKYKPTTPGRRGASVSDFAEITRSTPEKSLVRPLHGKGGRNAHGRITTRHKGGGHKRAYRVIDFRRHDKDGVNAKVAHIEYDPNRTANIALLHYLDGEKRYIIAPQGLKQGDVIESGANADIKPGNNLPLRNIPAGTVIHAVELRPGGGAKLARSAGVSIQLLGKEGTYAALRMPSGEIRRVDVRCRATVGEVGNAEQSNINWGKAGRMRWKGKRPTVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGKPEGRTRKPNKPSDKLIVRRRRTGK

Entity 11 polymer

描述 description	50S ribosomal protein L3
分子量 formula_weight	22621.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

ARKGILGTKLGMTQVFDENNKVVPVTVVKAGPNVVTRIRTTERDGYSAVQLAYGEISPRKVIKPVAGQFAAAGVNPRRHVAELRLDDEAAVAEYEVGQELTAEIFSDGAYVDVTGTSKGKGFAGTMKRHGFRGQGAAHGAQAVHRRPGSIGGCATPGRVFKGTRMSGRMGNDRVTTQNLKVHKVDAENGVLLIKGAIPGRNGGLVVVRSAIKRG

Entity 12 polymer

描述 description	50S ribosomal protein L4
分子量 formula_weight	22346.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

TLKVDVKTPAGKTDGSVELPAELFDVEPNIALMHQVVTAQLAAKRQGTHSTKTRGEVSGGGKKPYRQKGTGRARQGSTRAPQFTGGGTVHGPKPRDYSQRTPKKMIAAALRGALSDRARNDRIHAVTELVEGQTPSTKSAKTFLGTLTENKKVLVVIGRTDEVGAKSVRNLPGVHVISPDQLNTYDVLNADDVVFSVEALNAYISANSK

Entity 13 polymer

描述 description	50S ribosomal protein L5
分子量 formula_weight	20588.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

KALPRLKQRYREEIREALQQEFNYANVMQIPGVVKVVVNMGVGDAARDAKLINGAINDLALITGQKPEVRRARKSIAQFKLREGMPIGARVTLRGDRMWEFLDRLISIALPRIRDFRGLSPKQFDGTGNYTFGLNEQSMFHEIDVDSIDRPRGMDITVVTTATNDAEGRALLRALGFPFKEN

Entity 14 polymer

描述 description	50S ribosomal protein L6
分子量 formula_weight	19165.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P

SRIGKQPVPVPSGVDVTINGQNLSVKGPKGTLTLDVAEPISVSRAEDGAIVVTRPDDERRSRSLHGLSRTLIANLVTGVTEGYTQKMEIFGVGYRVQLKGQNLEFALGYSHPVLIEAPEGITFAVESPTKFSVSGIDKQKVGQISAVIRRLRRPDPYKGKGVRYEGEQIRRKVGKT

Entity 15 polymer

描述 description	50S ribosomal protein L9
分子量 formula_weight	15949.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q

MKLILTAEVEHLGAAGDTVEVKDGYGRNYLLPRGLAIVASRGAERQAEEIRRARESKVIRDIEHANELKTALEGLGDVTLSVNAAGDTGKLFGSVTAADVVNAIKKAGGPNLDKRTVQLAKAHIKSVGTHPVTVKLHTGVEAKVSLNVVAQ

Entity 16 polymer

描述 description	50S ribosomal protein L10
分子量 formula_weight	13095.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R

MAKADKATAVADIAEQFKASTATVVTEYRGLTVANLAELRRALGDSATYTVAKNTLVKRAASEAGIEGLDELFAGPTAIAFVKGEAVDAAKAIKKFAKDNKALVIKGGYMDGKALSVADVEKIADL

Entity 17 polymer

描述 description	50S ribosomal protein L11
分子量 formula_weight	14109.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S

LIKLQIQAGQANPAPPVGPALGQHGVNIMEFCKAYNAATESQRGNVIPVEITVYEDRSFTFALKTPPAAKLLLKAAGVQKGSGEPHKTKVAKVTWDQVREIAETKKADLNANDIDAAAKIIAGTARSMGITVE

Entity 18 polymer

描述 description	50S ribosomal protein L13
分子量 formula_weight	16015.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T

PTYTPKAGDTTRSWYVIDASDVVLGRLASAAATLLRGKHKPTFTPNVDGGDFVIVINADKIAVSGDKLTKKFAYRHSGYPGGLRKRTIGELLEKHPTRVVENAIIGMLPHNKLGRQIQKKLKVYAGPDHPHAAQQPIPFEIKQVAQ

Entity 19 polymer

描述 description	50S ribosomal protein L14
分子量 formula_weight	13400.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	U

MIQQESRLKVADNTGAKEILCIRVLGGSSRRYAGIGDVIVATVKDAIPGGNVKRGDVVKAVVVRTVKERRRADGSYIKFDENAAVIIKNDNDPRGTRIFGPVGRELREKKFMKIVSLAPEVL

Entity 20 polymer

描述 description	50S ribosomal protein L15
分子量 formula_weight	15384.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	V

VIKLHDLKPAPGEKKAKTRVGRGEGSKGKTAGRGTKGTKARKNVPVMFEGGQMPIHMRLPKLKGFKNRFRTEYQVVNVGDINKAFPQGGTVGVDELVAKGLVRKNSLVKVLGDGKLTVKVDVTANKFSGSAREAITAAGGSATEL

Entity 21 polymer

描述 description	50S ribosomal protein L17
分子量 formula_weight	13210.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J

PKPTKGPRLGGSSSHQSALLANLATSLFEHGRIKTTEPKARALRPYAEKLITHAKKGALHNRREVMKKIRDKDVVHTLFAEIGPFYADRNGGYTRIIKVENRKGDNAPMAVIELVREK

Entity 22 polymer

描述 description	50S ribosomal protein L18
分子量 formula_weight	13580.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

AHKPVGQNISEVRRNARLRRHARLRKKVAGTAEVPRLVVNRSARHIHVQLVNDLNGTTLAAASSIEADVRAIDGDKKAHSVRVGQLIAERAKAAGVETVVFDRGGYTYGGRIAALADAAREAGLKF

Entity 23 polymer

描述 description	50S ribosomal protein L19
分子量 formula_weight	12892.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MNTLDFVDQASLRDDIPTFSPGDTVNVHVKVIEGSKERIQVFKGVVIRRQGGGISETFTVRKESYGVGVERTFPVHSPNIDHIDVLTRGDVRRAKLYYLRELRGKKAKIKEKR

Entity 24 polymer

描述 description	50S ribosomal protein L20
分子量 formula_weight	14035.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	W

ARVKRALNAQKKRRTVLKASKGYRGQRSRLYRKAKEQQLHSLTYAYRDRRARKGEFRKLWISRINAAARANDITYNRLIQGLKAAGVEVDRKNLAELAVSDPAAFTALVDVARAALPEDVNAPS

Entity 25 polymer

描述 description	50S ribosomal protein L21
分子量 formula_weight	10724.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	X

TYAIVKTGGKQYKVAAGDVVKVEKLDSEPGASVSLPVALVVDGANVTSKADDLAKVAVTAEVLEHTKGPKIRIHKFKNKTGYHKRQGHRQQLTVLKVTGI

Entity 26 polymer

描述 description	50S ribosomal protein L22
分子量 formula_weight	12408.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Y

EFPSATAKARYVRVSATKARRVIDLVRGKSVEEALDILRWAPQAASEPVAKVIASAAANAQNNEGLDPSTLVVATVYADEGPTAKRIRPRAQGRAFRIRKRTSHITVIVESRPP

Entity 27 polymer

描述 description	50S ribosomal protein L23
分子量 formula_weight	10748.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Z

ATITDPRDIILAPVISEKSYGLIEDNVYTFVVHPDSNKTQIKIAIEKIFDVKVDSVNTANRQGKRKRTRTGFGKRKSTKRAIVKLAAGSKPIDLFGA

Entity 28 polymer

描述 description	50S ribosomal protein L24
分子量 formula_weight	11228.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1

MKVHKGDTVLVISGKDKGAKGKVLVAYPDRNKVLVEGVNRIKKHTAVSANERGASSGGIVTQEAPIHVSNVMVVDSDGKPTRVGYRIDDETGKKVRIAKTNGKDI

Entity 29 polymer

描述 description	50S ribosomal protein L25
分子量 formula_weight	20331.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2

TANIPNKLTANVRTRTGKGASRQARRDGKVPAVLYGHGTDPQHLELNARDFAAVLRSHGTNAILTLDIEGTEQLALTKALDVHPIRRNIQHADLLVVQRGEKVTVEVTVLVEGDATPGTLVTQDANTIEIEAEALSIPEQLTVSVEGVEAGTQITAGQISLPEGVNLISDPELLVVNVVEAPSAEALEEEGA

Entity 30 polymer

描述 description	50S ribosomal protein L27
分子量 formula_weight	8322.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	4

SSSRNGRDSAAQRLGVKRFGGQVVKAGEILVRQRGTHFHPGVNVGRGGDDTLFALAPGAVEFGAKRGRKTVNIVPVARP

Entity 31 polymer

描述 description	50S ribosomal protein L29
分子量 formula_weight	7590.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	6

GTTPGELRELTDDELKDKLRESKEELFNLRFQMATGQLSNNRRLRTVRQEIARVYTVLRERELG

Entity 32 polymer

描述 description	50S ribosomal protein L30
分子量 formula_weight	6761.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	7

AELKITQVRSTIGARWKQRESLRTLGLKKIRQSVVREDNAQTRGLINTVHHLVEVEEVG

Entity 33 polymer

描述 description	50S ribosomal protein L32
分子量 formula_weight	6050.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	8

AVPKRRMSRANTRSRRAQWKAEAPGLVTVSVAGQQRKVPRRLLKAARLGLVDLD

Entity 34 polymer

描述 description	50S ribosomal protein L34
分子量 formula_weight	5391.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	0

AKGKRTFQPNNRRRARVHGFRLRMRTRAGRAIVANRRSKGRRALTA

Entity 35 polymer

描述 description	50S ribosomal protein L35
分子量 formula_weight	7167.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	x

PKAKTHSGASKRFRRTGTGKIVRQKANRRHLLEHKPTKRTRRLDGRTTVSAADNSRINKLLNG

Entity 36 polymer

描述 description	50S ribosomal protein L36
分子量 formula_weight	4341.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

MKVNPSVKPICDKCRVIRRHGRVMVICSDPRHKQRQG

Entity 37 polymer

描述 description	16S rRNA (1511-MER)
分子量 formula_weight	490111.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	a

UUUGGAGAGUUUGAUCCUGGCUCAGGACGAACGCUGGCGGCGUGCUUAACACAUGCAAGUCGAACGGAAAGGCCCUUUCGGGGGUACUCGAGUGGCGAACGGGUGAGUAACACGUGGGUGAUCUGCCCUGCACUUUGGGAUAAGCCUGGGAAACUGGGUCUAAUACCGAAUACACCCUGCUGGUCGCAUGGCCUGGUAGGGGAAAGCUUUUGCGGUGUGGGAUGGGCCCGCGGCCUAUCAGCUUGUUGGUGGGGUGAUGGCCUACCAAGGCGACGACGGGUAGCCGGCCUGAGAGGGUGACCGGCCACACUGGGACUGAGAUACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCGACGCCGCGUGAGGGAUGACGGCCUUCGGGUUGUAAACCUCUUUCAGCACAGACGAAGCGCAAGUGACGGUAUGUGCAGAAGAAGGACCGGCCAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGGUCCGAGCGUUGUCCGGAAUUACUGGGCGUAAAGAGCUCGUAGGUGGUUUGUCGCGUUGUUCGUGAAAACUCACAGCUUAACUGUGGGCGUGCGGGCGAUACGGGCAGACUAGAGUACUGCAGGGGAGACUGGAAUUCCUGGUGUAGCGGUGGAAUGCGCAGAUAUCAGGAGGAACACCGGUGGCGAAGGCGGGUCUCUGGGCAGUAACUGACGCUGAGGAGCGAAAGCGUGGGGAGCGAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGGUGGGUACUAGGUGUGGGUUUCCUUCCUUGGGAUCCGUGCCGUAGCUAACGCAUUAAGUACCCCGCCUGGGGAGUACGGCCGCAAGGCUAAAACUCAAAGGAAUUGACGGGGGCCCGCACAAGCGGCGGAGCAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGGUUUGACAUGCACAGGACGCCGGCAGAGAUGUCGGUUCCCUUGUGGCCUGUGUGCAGGUGGUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUGUCUCAUGUUGCCAGCACGUUAUGGUGGGGACUCGUGAGAGACUGCCGGGGUCAACUCGGAGGAAGGUGGGGAUGACGUCAAGUCAUCAUGCCCCUUAUGUCCAGGGCUUCACACAUGCUACAAUGGCCGGUACAAAGGGCUGCGAUGCCGUGAGGUGGAGCGAAUCCUUUCAAAGCCGGUCUCAGUUCGGAUCGGGGUCUGCAACUCGACCCCGUGAAGUCGGAGUCGCUAGUAAUCGCAGAUCAGCAACGCUGCGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACGUCAUGAAAGUCGGUAACACCCGAAGCCGGUGGCCUAACCCUUGUGGAGGGAGCCGUCGAAGGUGGGAUCGGCGAUUGGGACGAAGUCGUAACAAGGUAGCCGUACCGGAAGGUGCGGCUGGAUC

Entity 38 polymer

描述 description	30S ribosomal protein S22
分子量 formula_weight	4033.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	b

GSVIKKRRKRMSKKKHRKLLRRTRVQRRKLGK

Entity 39 polymer

描述 description	30S ribosomal protein S3
分子量 formula_weight	23617.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

GQKINPHGFRLGITTEWKSRWYADKQYKDYVKEDVAIRKLLATGLERAGIADVEIERTRDRVRVDIHTARPGIVIGRRGTEADRIRADLEKLTGKQVQLNILEVKNPESQAQLVAQGVAEQLSNRVAFRRAMRKAIQSAMRQPNVKGIRVQCSGRLGGAEMSRSEFYREGRVPLHTLRADIDYGLYEAKTTFGRIGVKVWIYKGDIVG

Entity 40 polymer

描述 description	30S ribosomal protein S4
分子量 formula_weight	23284.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	e

ARYTGPATRKSRRLGVDLVGGDQSFEKRPYPPGQHGRARIKESEYRQQLQEKQKARFSYGVMEKQFRRYYEEANRQPGKTGDNLLRILESRLDNVVYRAGLARTRRMARQLVSHGHFLVNGVKVDIPSYRVSQYDIIDVKEKSLNTLPFQIARETAGERPIPSWLQVVGERQRILVHQLPERAQIDVPLTEQLIVELYSK

Entity 41 polymer

描述 description	30S ribosomal protein S5
分子量 formula_weight	18481.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	f

NYIERVVSINRVSKVVKGGRRFSFTALVIVGDGKGMVGVGYGKAKEVPAAIAKGVEEARKNFFRVPLIGSTITHPVQGEAAAGVVMLRPASPGTGVIAGGAARAVLECAGVHDILAKSLGSDNAINVVHATVAALKLLQRPEEVAARRGLPIEDVAPAGMLKARRESEALAAAAAREGSA

Entity 42 polymer

描述 description	30S ribosomal protein S6
分子量 formula_weight	10991.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	g

MRPYEIMVILDPTLDERTVAPSLETFLNVIRKDGGTVDKVDIWGRRRLAYEIAKHAEGIYAVIDVKAEPATVSELDRQLNLNESVLRTKVLRTDKH

Entity 43 polymer

描述 description	30S ribosomal protein S7
分子量 formula_weight	17529.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	h

PRKGPAPKRPLVNDPVYGSQLVTQLVNKVLLEGKKSLAERIVYGALEQAREKTGTDPVVTLKRALDNVKPALEVRSRRVGGATYQVPVEVRPDRSTTLALRWLVNFSRQRREKTMVERLANEILDASNGLGASVKRREDTHKMAEANRAFAHYRW

Entity 44 polymer

描述 description	30S ribosomal protein S8
分子量 formula_weight	14361.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	i

TMTDPIADFLTRLRNANSAYHDEVTLPHSKLKANIAEILKREGYISDYRTEDARVGKSLVVQLKYGPSRERSIAGLRRVSKPGLRVYAKSTNLPRVLGGLGVAIISTSSGLLTDRQAARQGVGGEVLAYVW

Entity 45 polymer

描述 description	30S ribosomal protein S9
分子量 formula_weight	14093.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	k

PIQTVGRRKEAVVRVRLVPGTGQFNLDGRTLENYFPNKVHQQLIKAPLVTVDRVDQFDIYAHLDGGGPSGQAGALRLAIARALILVQPEDRPALKKAGFLTRDPRAIERKKYGLKKARKAPQYSKR

Entity 46 polymer

描述 description	30S ribosomal protein S10
分子量 formula_weight	11252.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	l

GQKIRIRLKAYDHEAIDASARKIVETVTRTGASVVGPVPLPTEKNVYCVIRSPHKYKDSREHFEMRTHKRLIDILDPTPKTVDALMRIDLPASVDVNIQ

Entity 47 polymer

描述 description	30S ribosomal protein S11
分子量 formula_weight	12151.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	m

KNVPHGAAHIKSTFNNTIVSITDPQGNVIAWASSGHVGFKGSRKSTPFAAQLAAENAARKAQEHGVKKVDVFVKGPGSGRETAIRSLQAAGLEVGTISDVTPQPHNGCRPPKRRR

Entity 48 polymer

描述 description	30S ribosomal protein S12
分子量 formula_weight	13678.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	n

PTIQQLVRKGRRDKIAKVKTAALKGSPQRRGVCTRVYTTTPKKPNSALRKVARVKLTSQVEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGSLDTQGVKNRKQARSRYGAKKEK

Entity 49 polymer

描述 description	30S ribosomal protein S15
分子量 formula_weight	10236.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	p

ALTAEQKKEILGQYGLHDTDTGSPEAQVALLTKRIQDLTEHLKVHKHDHHSRRGLLLLVGRRRRLLKYVAQVDVARYRSLIERLGLRR

Entity 50 polymer

描述 description	30S ribosomal protein S16
分子量 formula_weight	12635.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	q

AVKIKLTRLGKIRNPQYRIIVADARTRRDGRAIEVIGRYHPKEEPSLIQIDSERAQYWLGVGAQPTEPVLALLKITGDWQKFKGLPGAEGTLKVKEPKPSKLDLFNAALAEAE

Entity 51 polymer

描述 description	30S ribosomal protein S17
分子量 formula_weight	10680.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	r

QKGPKYTPAAEKPRGRRKTAIGYVVSDKMQKTIVVELEDRKSHPLYGKIIRTTKKVKAHDENGEAGIGDRVSLMETRPLSATKRWRLVEILEKA

Entity 52 polymer

描述 description	30S ribosomal protein S20
分子量 formula_weight	9424.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	t

ANIKSQIKRIRTNERRRLRNQSVKSSLRTAIRGFREAVDAGDKDKASELLHATSRKLDKAASKGVIHPNQAANKKSALALALNKL

Entity 53 polymer

描述 description	30S ribosomal protein S2
分子量 formula_weight	25576.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	u

MAVVTMKQLLDSGAHFGHQTRRWNPKMKRFIFTDRNGIYIIDLQQTLTYIDKAYEFVKETVAHGGTVLFVGTKKQAQESIAEEATRVGMPYVNQRWLGGMLTNFSTVHKRLQRLKELEAMEQTGGFEGRTKKEILMLTREKNKLERSLGGIRDMQKVPSAVWVVDTNKEHIAVGEARKLGIPVIAILDTNCDPDVVDYPIPGNDDAIRSAALLTKVIASAVAEGLQAR

Entity 54 polymer

描述 description	mRNA
分子量 formula_weight	6503.0 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	v

AGGAGGUAAAAAUGUUCAAA

Entity 55 polymer

描述 description	GTP pyrophosphokinase RelA
分子量 formula_weight	88057.7 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	I

MVDEPGKGQAVQSPPAVPETVPKPPSGTAPTTTPAAPSTSSETARISASRRVRARLARRMTAQRSAINPVLEPLVAVHREIYPKADLQLLQRAYEVAEKRHADQLRKSGDPYITHPLAVANILAELGMDTTTLVAALLHDTVEDTGYSLEALTADFGSEVGHLVDGVTKLDKVVLGSAAEGETIRKMIIAMARDPRVLVIKVADRLHNMRTMRFLPPEKQARKARETLEVIAPLAHRLGMATVKWELEDLAFAILHPKKYEEIVRLVADRAPSRDTYLAKVRAEIGVALSAMKINAVVEGRPKHYWSIYQKMIVKGRDFDDIHDLVGVRILCDEIRDCYAAVGVVHSLWQPMAGRFKDYIAQPRYGVYQSLHTTVVGPEGKPLEVQIRTREMHRTAEYGIAAHWRYKEAKGRNGVPHSHAAAEIDDMAWMRQLLDWQREAADPGEFLESLRYDLATQEIFVFTPKGDVITLPAGSTPVDFAYAVHTEVGHRCIGARVNGRLVALERKLENGEVVEVFTSKAPNAGPSRDWQGFVVSPRAKTKIRQWFAKERREEALESGKDAIAREVRRGGLPLQRLMNAETMGALARELRYQDVSALYTAVGEGHVSARHVVQRLLAQFGGDDAAEDELAERSTPATMPMRQRSNDDTGVAVPGAPGVLTKLAKCCTPVPGDTIMGFVTRGGGVSVHRTDCTNAESLQQQSERIIEVNWAPSPSSVFLVAIQVEALDRHRLLSDVTRVLADEKVNILSASVTTSNDRVAISRFTFEMGDPKHLGHLLSVVRNVEGVYDVYRVTSAA

来源 Source

来源生物 organism	Mycolicibacterium smegmatis MC2 155
NCBI 分类编号 ncbi_taxonomy_id	246196
宿主生物 host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 56 polymer

描述 description	50S ribosomal protein L31
分子量 formula_weight	9141.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H

MKPGIHPDYHPVVFQDAATGAQFLTRSTATSTRTIEWPTPSGPKTYPLIVVDVTSDSHPFWTGSARHIDSAGQVEKFRRRYG

Entity 57 polymer

描述 description	30S ribosomal protein S18 1
分子量 formula_weight	9449.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	o

MMAVKKSRKRTAATELKKPRRNQLEALGVTTIDYKDVAVLRTFLSERGKIRSRHVTGLTPQQQRQVATAIKNAREMALLPMAGP

Entity 58 polymer

描述 description	30S ribosomal protein S14
分子量 formula_weight	11661.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	9

AKKSKIVKNEQRRELVQRYAERRAELKRTIRDPASSPERRAAAVSALQRLPRDSSPVRLRNRDVVDGRPRGHLRKFGLSRVRVREMAHRGELPGVRKASW

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.76 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	36321

fit	/app/data/saxs_computation/fr/8fr8/8fr8.fit
pddf	/app/data/pr_computation/fr/8fr8/8fr8.pr.out
profile	/app/data/saxs_computation/fr/8fr8/8fr8.dat

Structure of Mycobacterium smegmatis Rsh bound to a 70S translation initiation complex

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (58)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 polymer

聚合体 Polymer

Entity 34 polymer

聚合体 Polymer