8gmo

Bacteriophage T4 capsid shell containing 9DE insertions into the gp23* major capsid protein subunits

Method: ELECTRON MICROSCOPY D_max: 636.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8gmo
沉积日期 deposition_date	2023-03-26
结构标题 title	Bacteriophage T4 capsid shell containing 9DE insertions into the gp23* major capsid protein subunits
关键词 keywords	Bacteriophage T4, capsid shell, phage display, decorated capsid, phage head, VIRUS LIKE PARTICLE; VIRUS LIKE PARTICLE
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	212.90 Å
零角强度 I(0) i0	323446000000.00
分子量 molecular_weight	4847500.0 kDa
排除体积 excluded_volume	6046200 Å³
包络体积 envelope_volume	15008000 Å³
水化壳体积 shell_volume	622970 Å³
包络直径 envelope_diameter	698.3 Å
壳层 Rg shell_rg	169.10 Å
包络 Rg envelope_rg	198.30 Å
形状 Rg shape_rg	212.80 Å
总 Rg total_rg	212.80 Å
总原子数 total_atoms	341379
残基数 n_residues	45294
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	636.6 Å
Rg (实空间) rg_real	218.90 Å
Rg 误差 (实空间) rg_real_error	3.17 Å
I(0) (实空间) i0_real	3.2150e+11
I(0) 误差 (实空间) i0_real_error	8.5710e+09
Rg (倒空间) rg_reciprocal	146.50 Å
I(0) (倒空间) i0_reciprocal	232700000000.0000
解质量估计 total_estimate	0.8891
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	272.8 Å
偏度 Skewness skewness	0.238
峰度 Kurtosis kurtosis	-0.860
角度范围 angular_range	— – 0.0350 Å⁻¹
当前正则化参数 α current_alpha	2.5700
最高正则化参数 α highest_alpha	2350000000.0000
实空间数据点数 n_real_points	8
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 10.070; Oscil: 0.979; Stabil: 0.914; Sysdev: 1.000; Positv: 1.000; Valcen: 0.891; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Mature major capsid protein
分子量 formula_weight	49790.7 kDa
来源方法 src_method	man
拷贝数 entity_count	93

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	;0,1,2,3,4,5,6,7,8,9,AA,AB,AC,AD,AE,AF,AG,AH,AI,AJ,AK,AL,AM,AN,AO,AP,AQ,AR,AS,AT,AU,AV,AW,AX,AY,AZ,Aa,Ab,Ac,Ad,Ae,Af,Ag,Ah,Ai,Aj,Ak,Al,Am,G,H,J,K,L,M,N,O,P,Q,R,S,T,U,V,W,X,Y,Z,b,c,d,e,f,g,h,i,j,k,l,m,n,o,p,q,r,s,t,u,v,w,x,y,z ;
原子数 atom_count	341379

AEIGGDHGYNATNIAAGQTSGAVTQIGPAVMGMVRRAIPNLIAFDICGVQPMNSPTGQVFALRAVYGKDPVAAGAKEAFHPMYGPDAMFSGQGAAKKFPALAASTQTTVGDIYTHFFQETGTVYLQASVQVTIDAGDEDEDEDEDATDAAKLDAEIKKQMEAGALVEIAEGMATSIAELQEGFNGSTDNPWNEMGFRIDKQVIEAKSRQLKAAYSIELAQDLRAVHGMDADAELSGILATEIMLEINREVVDWINYSAQVGKSGMTLTPGSKAGVFDFQDPIDIRGARWAGESFKALLFQIDKEAVEIARQTGRGEGNFIIASRNVVNVLASVDTGISYAAQGLATGFSTDTTKSVFAGVLGGKYRVYIDQYAKQDYFTVGYKGPNEMDAGIYYAPYVALTPLRGSDPKNFQPVMGFKTRYGIGINPFAESAAQAPASRIQSGMPSILNSLGKNAYFRRVYVKGI

来源 Source

来源生物 organism	Tequatrovirus T4
NCBI 分类编号 ncbi_taxonomy_id	10665
宿主生物 host_organism	Escherichia coli

Entity 2 polymer

描述 description	Mature capsid vertex protein
分子量 formula_weight	45594.4 kDa
来源方法 src_method	man
拷贝数 entity_count	5

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,a

STTTNSNSIGRPNLVALTRATTKLIYSDIVATQRTNQPVAAFYGIKYLNPDNEFTFKTGATYAGEAGYVDREQITELTEESKLTLNKGDLFKYNNIVYKVLEDTPFATIEESDLELALQIAIVLLKVRLFSDAASTSKFESSDSEIADARFQINKWQTAVKSRKLKTGITVELAQDLEANGFDAPNFLEDLLATEMADEINKDILQSLITVSKRYKVTGITDSGFIDLSYASAPEAGRSLYRMVCEMVSHIQKESTYTATFCVASARAAAILAASGWLKHKPEDDKYLSQNAYGFLANGLPLYCDTNSPLDYVIVGVVENIGEKEIVGSIFYAPYTEGLDLDDPEHVGAFKVVVDPESLQPSIGLLVRYALSANPYTVAKDEKEARIIDGGDMDKMAGRSDLSVLLGVKLPKIII

来源 Source

来源生物 organism	Tequatrovirus T4
NCBI 分类编号 ncbi_taxonomy_id	10665
宿主生物 host_organism	Escherichia coli

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	triclinic

分辨率 resolution	3.90 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	70340

fit	/app/data/saxs_computation/gm/8gmo/8gmo.fit
pddf	/app/data/pr_computation/gm/8gmo/8gmo.pr.out
profile	/app/data/saxs_computation/gm/8gmo/8gmo.dat

Bacteriophage T4 capsid shell containing 9DE insertions into the gp23* major capsid protein subunits

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)