8gzg

Cryo-EM structure of Synechocystis sp. PCC 6803 RPitc

Method: ELECTRON MICROSCOPY D_max: 189.0 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8gzg
沉积日期 deposition_date	2022-09-27
结构标题 title	Cryo-EM structure of Synechocystis sp. PCC 6803 RPitc
关键词 keywords	;Cryo-EM, Synechocystis sp. PCC 6803, Syn6803, Transcription initiation, RPitc, Transcription, Cyanobacteria, TRANSCRIPTION-DNA-RNA complex ;; TRANSCRIPTION/DNA/RNA
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	52.33 Å
回转半径 Rg (电子) rg_electron	51.99 Å
零角强度 I(0) i0	2821880000.00
分子量 molecular_weight	420700.0 kDa
排除体积 excluded_volume	517250 Å³
包络体积 envelope_volume	801930 Å³
水化壳体积 shell_volume	123720 Å³
包络直径 envelope_diameter	204.9 Å
壳层 Rg shell_rg	58.57 Å
包络 Rg envelope_rg	52.07 Å
形状 Rg shape_rg	52.02 Å
总 Rg total_rg	52.07 Å
总原子数 total_atoms	29503
残基数 n_residues	3723
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	189.0 Å
Rg (实空间) rg_real	52.26 Å
Rg 误差 (实空间) rg_real_error	1.54 Å
I(0) (实空间) i0_real	2.8220e+09
I(0) 误差 (实空间) i0_real_error	5.2840e+07
Rg (倒空间) rg_reciprocal	52.39 Å
I(0) (倒空间) i0_reciprocal	2822000000.0000
解质量估计 total_estimate	0.7658
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	63.0 Å
偏度 Skewness skewness	0.378
峰度 Kurtosis kurtosis	-0.031
角度范围 angular_range	— – 0.1500 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	585600000.0000
实空间数据点数 n_real_points	31
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.657; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.980; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (11)

Entity 1 polymer

描述 description	DNA-directed RNA polymerase subunit alpha
分子量 formula_weight	36657.3 kDa
EC 编号 ec_number	2.7.7.6
来源方法 src_method	man
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	29503

MGSSHHHHHHSQDPMAQFQIECVESSTRKNQQQYSKFSLEPLDRGQGTTVGNALRRVLLSNLPGAAVTAIRIAGVNHEFATILGVREDVLEIMLNMKELVLKSYTDQPQIGRLTAIGPGTVTAAQFEVPSEVEVIDPNQYIATLAEGAKLEMEFRVERGVGYRVIERGKDENSSLDFLQIDSVFMPVTKVNYTVEDIRADGMSPKDRLILDIWTNGSIQPREALSEASDIIANLFIPLKDLNELEAAHSDYQDEVNPESQIPIEELQLSVRAYNCLKRAQINSVADLLEYSQEDLLEIKNFGLKSAEEVIEALQKRLGITLPHEKAKA

来源 Source

来源生物 organism	Synechocystis sp. PCC 6803
NCBI 分类编号 ncbi_taxonomy_id	1148
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

描述 description	DNA-directed RNA polymerase subunit beta
分子量 formula_weight	123719.8 kDa
EC 编号 ec_number	2.7.7.6
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MAMTNLATTMLPDLIEIQHASFHWFLEEGLIEELNSFSPISDYTGKLELHFLGKDYKLKQPKYDVDESKRRDASYSVQMYVPTRLINKETGEIKEQEVFIGDLPLMTERGTFIINGAERVIVNQIVRSPGVYYKKELDKNGRRTYSASLIPNRGAWLKFETDKNGLVYVRIDKTRKLSAQVLLKAIGLSDNEILDSLSHPEFYQKTLDKEGNPTEEEALVELYKKLRPGEPPTVSGGQQLLESRFFDPKRYDLGRVGRYKLNKKLRLNEADTTRVLTPQDILAAINYLINLEFDVGTTDDIDHLGNRRVRSVGELLQNQIRVGLNRLERIIRERMTVSESDALTPASLVNPKPLVAAIKEFFGSSQLSQFMDQTNPLAELTHKRRISALGPGGLTRERAGFAVRDIHPSHHGRICPVETPEGPNAGLIGSLATCARVNDYGFIETPYFRVESGRVRKDLDPVYLTADEEDDMRVAPGDIPTDEEGNIIGESVPIRYRQEFSTTSPEQVDYVAVSPVQIISVATSMIPFLEHDDANRALMGSNMQRQAVPLLRPERPLVGTGLEAQAARDSGMVIVSRTHGIVTYVDATEIRVQPHSPDNPAEKGEEIVYPIQKYQRSNQDTCLNQRPLVYAGEDVVPGQVLADGSATEGGELALGQNILVAYMPWEGYNYEDAILISERLVYDDVYTSIHIEKFEIEARQTKLGPEEITREIPNVGEDALRNLDEHGIIRIGAWVESGDILVGKVTPKGEADQPPEEKLLRAIFGEKARDVRDNSLRVPNGEKGRVVDVRVFTREKGDELPPGANMVVRIYVAQKRKIQVGDKMAGRHGNKGIISRILPIEDMPYLPDGRPIDIALNPLGVPSRMNVGQVFECLLGWAGENLGVRFKITPFDEMYGEEASRDTVHGLLEEASQRPNKDWVFNENHPGKIQVFDGRTGEPFDRPITVGQAYMLKLVHLVDDKIHARSTGPYSLVTQQPLGGKAQQGGQRFGEMEVWALEAYGAAYILQELLTVKSDDMQGRNEALNAIVKGKSIPRPGTPESFKVLMRELQSLGLDIAAHKVQLSEDGESADAEVDLMIDSQRRAPNRPTYESLHTEEDLEEEEV

来源 Source

来源生物 organism	Synechocystis sp. PCC 6803
NCBI 分类编号 ncbi_taxonomy_id	1148
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 3 polymer

描述 description	DNA-directed RNA polymerase subunit gamma
分子量 formula_weight	71073.0 kDa
EC 编号 ec_number	2.7.7.6
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MKAQSEPRFDYVKIAIASPERIRQWGERTLPNGTVVGEVTKPETINYRTLKPEMDGLFCEKIFGPSKDWECWCGKYKRVRHRGIVCERCGVEVTESRVRRHRMGYIKLAAPVTHVWYLKGIPSYLSILLDMALRDVEQIVYFNAYVVLNPGNASNLQYKQLLTEDQWVEIEDQIYAEDSELEGIEVGIGAEAVQRLLAELQLEEVAEKLREEILASKGQKRAKLIKRLRVIDNFIATHSQAEWMTLDVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLARLQEILAPEIIVRNEKRMLQEAVDALIDNGRRGRTVVGANNRALKSLSDIIEGKQGRFRQNLLGKRVDYSGRSVIVVGPNLKIYQCGLPREMAIELFQPFVIHRLIKLGIVNNIKAAKKLILKGDPQIWSVLEEVITGHPVMLNRAPTLHRLGIQAFEPILVEGRAIQLHPLVCPAFNADFDGDQMAVHVPLSLEAQCEARLLMLACHNVLSPATGKPIVAPSQDMVLGCYYLTAENPNAQKGAGRYFAGIEDALRAYDHGQVDLHSQIWIRHLDEDVVTEKPDTEVIKTEDLGDGTVMKYYRERKIREGVDGEIITQYIQTTPGRIIYNKTIAEALVF

来源 Source

来源生物 organism	Synechocystis sp. PCC 6803
NCBI 分类编号 ncbi_taxonomy_id	1148
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 4 polymer

描述 description	DNA-directed RNA polymerase subunit omega
分子量 formula_weight	8749.8 kDa
EC 编号 ec_number	2.7.7.6
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MTKRSNLDSNHIIYRSEELLGAASNRYNITVRVAKRAKENRSEDFDSIDDPNMKPAIRAIIEMSDELTRPEIISDN

来源 Source

来源生物 organism	Synechocystis sp. PCC 6803
NCBI 分类编号 ncbi_taxonomy_id	1148
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 5 polymer

描述 description	RNA polymerase sigma factor SigA
分子量 formula_weight	50335.4 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

GAMGMTQTKEPLTKAESAELEQEIELSQYINTDDIDDDDIDVEDLEQEVAATEGKEKKVRKIRKDAVKKKPYTEDSIRIYLQEIGRIRLLRAEEEIELARQIADLLELELIRDNLTLQLERQPSELEWGKQVWKLETAKQRLVGDKKKEPKKKDIDSYLANPDNELSLENEWSQQPNKNFAAFRRRLFLDRRAKDKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMRRKPTEEEIAEKMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADGETPEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSILKEYIR

来源 Source

来源生物 organism	Synechocystis sp. PCC 6803
NCBI 分类编号 ncbi_taxonomy_id	1148
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 6 polymer

描述 description	;DNA-directed RNA polymerase subunit beta' ;
分子量 formula_weight	145372.0 kDa
EC 编号 ec_number	2.7.7.6
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Z

GSGSGSMTFYNYTIDKGRLKKLIALAYRRYGSARCSQLADELKELGFRFATKAGVSISVDDLTIPPEKKQMLEAAEKEIRTTEERYARGEITEVERFQKVIDTWNGTSEELKDQVVVNFRKTDPLNSVYMMAFSGARGNMSQVRQLVGMRGLMADPQGEIIDLPIKTNFREGLTVTEYVISSYGARKGLVDTALRTADSGYLTRRLVDVSQDVIVREQDCGTERSLRVTAMTDGDQVKISLADRLFGRLLAKDVVGPDGEIIAKRNDEIDEALANRIAAVTDEVYVRSPLTCEAARSVCQNCYGWSLAHGHKVDLGEAVGIIAAQSIGEPGTQLTMRTFHTGGVFTGEVARQEKAPEDGTVKWGKGLSTRKVRTRHGEDAEQVEIAGDLIWKGEGKKAATQTYSLTPGSLLFVQDGQTVTAGQLMTEISLSKTQRSTERATKDVAGDLAGEVLFDRLVPEEKTDRQGNTTRIAQRGGLVWILSGEVYNLPPGAEPVVKNDEQVEVGSIMAETKLVTNDGGVVRLVSNREIEIITASVLLDQAQVKLESSGGREQYVIYTADKQRFLLKAAPGTKVQNHSIVAELIDDRYRTTTGGMIRYAGVEVAKGGRKQGYEVTKGGTLLWIPEETHEINKDISLLIVEDGQYVEAGTEVVKDIFCQSSGIVEVVQKNDILREIIIKPGDFYQDVDPGSVKIESGQLLQPGQDVFPGVTVSTLSQAEWIESPEGNGLLLRPVEEYKVFDEPAAPSQGSQNEEGGRQIELRSVQRLFYKDGDRVKSVEGAPLLSTQLVLEIYGSGNEGISHLSADIELQDDEEEDCQRLQLVILESLVLRRDQESDPLGGASKTRLLVQDGDQIPPGAVVARTEIQCKEAGTVRGIKEGQESIRRVLLERAADRLVVDLPSAPEVKPGQLLVAGQELVPGVKLEESGKVLEINGKGDNYQLVLRRARPYRVSPGAVLHIEDGDLVQRGDNLVLLVFERAKTGDIVQGLPRIEELLEARKPKEACVLARAPGVCQVEYLEDESVDIKVVEDDGTVSEYPLLPGQNAMVTDGQRIDVGHALTDGYNNPHEILDVFFSYYVDKDGCYQAALRGLQAAQKFLVNEVQTVYQSQGVDISDKHIEVIVRQMTAKVRIDDGGDTTMLPGELVELRQVEQVNEAMGITGSAPARYTPVLLGITKASLNTDSFISAASFQETTRVLTEAAIEGKSDWLRGLKENVIIGRLIPAGTGFSSHEEVLGLIETQDDIQGYMIEPIELPTTKKKASATKVKTKKVEADDDLLDDTRARAYAGTQLSQDDEEFEETYDTDEDDFDMDDDDDFGDDED

来源 Source

来源生物 organism	Synechocystis sp. PCC 6803
NCBI 分类编号 ncbi_taxonomy_id	1148
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 7 polymer

描述 description	Nontemplate strand DNA
分子量 formula_weight	20810.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polydeoxyribonucleotide
链编号 strand_id	1

GCTTGACAAGGCCCGTCCGTTATGGTATAATGGGAGCTGTCACGGATGCAGGTGGCTGGTTCTCGCG

Entity 8 polymer

描述 description	Template strand DNA
分子量 formula_weight	20673.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polydeoxyribonucleotide
链编号 strand_id	2

CGCGAGAACCAGCCACCTGCATCCGTGAGTCGAGGGTAATAACCATAACGGACGGGCCTTGTCAAGC

Entity 9 polymer

描述 description	;RNA (5'-R(UPCPGPA)-3') ;
分子量 formula_weight	1240.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	3

UCGA

Entity 10 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 11 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

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6. 折叠分类 (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

结构域编号 domain_id	8gzgC01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	1110 — Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3
同源超家族 H-superfamily homologous superfamily	10 — RNA polymerase Rpb2, domain 2

结构域编号 domain_id	8gzgC02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily	150 — RNA polymerase II, Rpb2 subunit, wall domain

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.13 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	157889

fit	/app/data/saxs_computation/gz/8gzg/8gzg.fit
pddf	/app/data/pr_computation/gz/8gzg/8gzg.pr.out
profile	/app/data/saxs_computation/gz/8gzg/8gzg.dat

Cryo-EM structure of Synechocystis sp. PCC 6803 RPitc

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (11)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 polymer

聚合体 Polymer

来源 Source

Entity 5 polymer

聚合体 Polymer

来源 Source

Entity 6 polymer

聚合体 Polymer

来源 Source

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 non-polymer

Entity 11 non-polymer

6. 折叠分类 (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)