8h8e

Structure of the dimeric Xenopus tropical acid-sensitive outwardly rectifying channel ASOR trimer bound with tRNA (closed state)

Method: ELECTRON MICROSCOPY D_max: 163.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8h8e
沉积日期 deposition_date	2022-10-22
结构标题 title	Structure of the dimeric Xenopus tropical acid-sensitive outwardly rectifying channel ASOR trimer bound with tRNA (closed state)
关键词 keywords	Chloride Channel tRNA Complex, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	53.33 Å
回转半径 Rg (电子) rg_electron	51.18 Å
零角强度 I(0) i0	791224000.00
分子量 molecular_weight	225700.0 kDa
排除体积 excluded_volume	278510 Å³
包络体积 envelope_volume	441210 Å³
水化壳体积 shell_volume	71145 Å³
包络直径 envelope_diameter	160.7 Å
壳层 Rg shell_rg	56.04 Å
包络 Rg envelope_rg	49.42 Å
形状 Rg shape_rg	51.07 Å
总 Rg total_rg	51.70 Å
总原子数 total_atoms	15818
残基数 n_residues	1798
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	163.8 Å
Rg (实空间) rg_real	53.25 Å
Rg 误差 (实空间) rg_real_error	1.53 Å
I(0) (实空间) i0_real	7.9120e+08
I(0) 误差 (实空间) i0_real_error	1.5690e+07
Rg (倒空间) rg_reciprocal	53.37 Å
I(0) (倒空间) i0_reciprocal	791300000.0000
解质量估计 total_estimate	0.8547
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	87.4 Å
偏度 Skewness skewness	0.053
峰度 Kurtosis kurtosis	-0.932
角度范围 angular_range	— – 0.1500 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	58820000.0000
实空间数据点数 n_real_points	31
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.899; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.985; Smooth: 0.424

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Proton-activated chloride channel
分子量 formula_weight	41031.8 kDa
来源方法 src_method	man
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F
原子数 atom_count	15818

MEAIRKELSRSYQELNEEAEPVAIDPEEAEDEEKEQEEAASAVAPDRDSDRSSPPVRFSRTCLKNFFSVLLILVYLLLMGVAVFLVYQTITDFRDKLKHPVMSVSYKEVNMYDAPGIALYPGKARLLSCEHHWYDHIPPLKDPGQPGENTCVTQDISYIDPYTNKTMKHALIVQGPRDVRRRELVFLQFHLNETKQDFSAIDYLLFSSYEAFLKSHDQVKFMQDCESSFSSWKFSGGFRTWVKMSLVKTKEEDGSQSVEFRQETSVVNFIDRRETPDKGDQLFFVVFEWKDPYIQEIQDIITANPWSMIALLCSVFLVLFKAADFAKLSVKWMIKVRRRHLKKRARELNHIS

来源 Source

来源生物 organism	Xenopus tropicalis
通用名 common_name	tropical clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8364
宿主生物 host_organism	Spodoptera frugiperda

Entity 2 polymer

描述 description	tRNA (75-MER)of Spodoptera frugiperda
分子量 formula_weight	24108.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	G

UCUUCGGUAGUAUAGUGGUCAGUAUCCCCGCCUGUCACGCGGGAGACCGGGGUUCGAUUCCCCGCCGGAGAGCCA

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.81 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	182807

fit	/app/data/saxs_computation/h8/8h8e/8h8e.fit
pddf	/app/data/pr_computation/h8/8h8e/8h8e.pr.out
profile	/app/data/saxs_computation/h8/8h8e/8h8e.dat

Structure of the dimeric Xenopus tropical acid-sensitive outwardly rectifying channel ASOR trimer bound with tRNA (closed state)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)