8imi

;A1-A2, A3-A4, B'1-B'2, C'1-C'2 cylinder in cyanobacterial phycobilisome from Anthocerotibacter panamensis (Cluster A) ;

Method: ELECTRON MICROSCOPY D_max: 227.0 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8imi
沉积日期 deposition_date	2023-03-07
结构标题 title	;A1-A2, A3-A4, B'1-B'2, C'1-C'2 cylinder in cyanobacterial phycobilisome from Anthocerotibacter panamensis (Cluster A) ;
关键词 keywords	Complex, PHOTOSYNTHESIS; PHOTOSYNTHESIS
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	85.68 Å
回转半径 Rg (电子) rg_electron	85.91 Å
零角强度 I(0) i0	13154200000.00
分子量 molecular_weight	993540.0 kDa
排除体积 excluded_volume	1249600 Å³
包络体积 envelope_volume	1837400 Å³
水化壳体积 shell_volume	176410 Å³
包络直径 envelope_diameter	279.2 Å
壳层 Rg shell_rg	85.78 Å
包络 Rg envelope_rg	82.83 Å
形状 Rg shape_rg	85.90 Å
总 Rg total_rg	85.93 Å
总原子数 total_atoms	69970
残基数 n_residues	8859
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	227.0 Å
Rg (实空间) rg_real	82.67 Å
Rg 误差 (实空间) rg_real_error	0.70 Å
I(0) (实空间) i0_real	1.2580e+10
I(0) 误差 (实空间) i0_real_error	2.0250e+08
Rg (倒空间) rg_reciprocal	84.07 Å
I(0) (倒空间) i0_reciprocal	13090000000.0000
解质量估计 total_estimate	0.8997
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	63.1 Å
偏度 Skewness skewness	0.213
峰度 Kurtosis kurtosis	-0.873
角度范围 angular_range	— – 0.0900 Å⁻¹
当前正则化参数 α current_alpha	1.3130
最高正则化参数 α highest_alpha	1103000000.0000
实空间数据点数 n_real_points	19
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.996; Stabil: 0.962; Sysdev: 1.000; Positv: 1.000; Valcen: 0.824; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (6)

Entity 1 polymer

描述 description	ApcE
分子量 formula_weight	127950.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	0
原子数 atom_count	69970

MPIKGTSGSNIARPRFYNTVMVETIEGANAEERYFNPGELSSMAGFFNDAQRRLAIVQILTTNAEAIVSRAAGRIFTGGSPMAFSVQQANRQKDAEKERVSARVVQAESEQPIGNEVVIEDKGGFLERLKSFFSYGGAEVETPGFRPIPIAVYGPERMQKSLRDLDWFLRYVNYSLVAGDSNMILLNCLGLREILEKACSIDATIVAVQEMRRAATGYLKSNDDKELVGSYFDVIIRSLNADKSDTPADVVRPSSPDRAGLVLPAIYALAGQSRPAFKMSRTLTSAEKERVVRAAYRQVFERDILAYGQSISYLDSKVKNGEISVKEFIRLLGKSELYRKQFFEPFINSRVLELAFKHFLGRAPESRTEVQNYYSIVAAQGLGGLVDALVDGEEYGRIFGEDTVPFIRDLGQEAQPSWNWGAAYSLYNYAAPRRKVPQFITLYADYVKPLPNQHPYGSGNDPLEIQFGAIFKSETKAPSARPAPIGKDVQRILIRSGNPITNERGNPAGGISDKTSLSPQIFKLTQDNRRIRGKSGKGSLITNAGAGSVEVNVQAVIRAAYQQVFGRQLYEGQHLSVSEIKLENGEISVKEFVRDLATSEIFRKLYWQNFYVCKSIEYIHRRLLGRPTYGRDETNRYYDLAFKKGFAGVVNAILDTMEYAEVFGDDVVPYERYVTPAGLNLRKLRAGTVPTLPSFEETPKFIEKGTAPDRALPQIRSAINQGVSKKRDQRKIFSTVGIQTSLASRTEFDALIRAAYRQVFERDMDSYRITEVFSVLETKLRNREITTKEFIQALASSDLYRKQFFEPYPPTKNVELSLKHLLGRATKDQAELRKYNQIIATQGFKPFINAILDSKEYGEVFGDGTVPYNRYPTLPAANFPNTEILYNQLTKQSAEVVVPSFKPVTSPRGMDMSQTPLMLQAMGDIAEAEQEVALQKPLFIQKGKALRGAEGDPYTIGTRRSPKPIFWVPQGGTNPTEFQNVIRAAYRQVFERDVPDYQRLSYPESRLKNGEISMREFIRQLAESDLYRKQFYEPYPNTKVIELLTKHFLGRAPQDQAEIQRYNRILAGKGLKVAIEEVLNSDEYTQLFGEDVVPFKRYPTLPTGTYLASVATNDEMIQQSGSSYSPSYAGYSYPFS

Entity 2 polymer

描述 description	ApcA2
分子量 formula_weight	17470.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	23

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,N,O,P,Q,R,S,a,b,c,d,e,f,n,o,p,q,r,s

MSVVTKAIVSADAEARYLSPGELDRIRGFVSSGERRLRVAQTLTESRERIIKQAGDQLFQKRPDLVSPGGNAYGAERTASCLRDLDYYLRLVTFGIVAGDVTPIEEIGVIGVKEMYRNLEVPLPGMVEAVKAMKSVATGLLSGDDSAEVGYYFDYLAGALA

Entity 3 polymer

描述 description	ApcB3
分子量 formula_weight	17673.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

MQDVIGKVIAEYDTKGKYLDAAALDLLRSYFDSGDLRLKAAQAITANAEVIVRAASAKALHYTPVTKPGGNMYYARRYASCIRDLDYFLRYATYAMLADNTTLLDEYVLKGLTETYRALGVPLDISVRAINALKEVVAGQVGPKAGQEMAKYFDHLAKGLG

Entity 4 polymer

描述 description	ApcB2
分子量 formula_weight	17524.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	23

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H,I,J,K,L,T,U,V,W,X,Y,g,h,i,j,k,l,t,u,v,w,x,y

MQDAITSVINTYDVQGKYFDTSAFDKLKAYYATGELRVRAAGTISANAATIIKEASAKLFSNQPDLVRPGGNAYTTRRYAACVRDMDYFLRYATYAMLAGDTSILDERVLNGLKETYNSLGVPISSTVQGIQAMKEVTGSLVGSGAAKEMGVYFDYLSSGLS

Entity 5 polymer

描述 description	ApcC2
分子量 formula_weight	7801.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M,Z,m,z

MTRLFKVTALIPSYKKVRGGRELQNTYFTKLVEYDRWFAEQQRIQKQGGKILSVKMVAGKPGLNTGVL

Entity 6 non-polymer

描述 description	PHYCOCYANOBILIN
分子量 formula_weight	588.7 kDa
来源方法 src_method	syn
拷贝数 entity_count	48

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7. 引用文献 (1)

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分辨率 resolution	2.59 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	1109579

fit	/app/data/saxs_computation/im/8imi/8imi.fit
pddf	/app/data/pr_computation/im/8imi/8imi.pr.out
profile	/app/data/saxs_computation/im/8imi/8imi.dat

;A1-A2, A3-A4, B'1-B'2, C'1-C'2 cylinder in cyanobacterial phycobilisome from Anthocerotibacter panamensis (Cluster A) ;

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (6)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)