8j0j

AtSLAC1 8D mutant in closed state

Method: ELECTRON MICROSCOPY D_max: 100.8 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8j0j
沉积日期 deposition_date	2023-04-11
结构标题 title	AtSLAC1 8D mutant in closed state
关键词 keywords	Stomatal closure, anion channel, phosphorylation-dependent activation, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	33.00 Å
回转半径 Rg (电子) rg_electron	32.07 Å
零角强度 I(0) i0	200580000.00
分子量 molecular_weight	128870.0 kDa
排除体积 excluded_volume	167380 Å³
包络体积 envelope_volume	208200 Å³
水化壳体积 shell_volume	51993 Å³
包络直径 envelope_diameter	100.4 Å
壳层 Rg shell_rg	40.76 Å
包络 Rg envelope_rg	32.21 Å
形状 Rg shape_rg	32.06 Å
总 Rg total_rg	32.86 Å
总原子数 total_atoms	9285
残基数 n_residues	1131
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	100.8 Å
Rg (实空间) rg_real	32.74 Å
Rg 误差 (实空间) rg_real_error	0.55 Å
I(0) (实空间) i0_real	2.0060e+08
I(0) 误差 (实空间) i0_real_error	3.2280e+06
Rg (倒空间) rg_reciprocal	32.86 Å
I(0) (倒空间) i0_reciprocal	200600000.0000
解质量估计 total_estimate	0.6865
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	0
主峰位置 r_peak_primary	— Å
偏度 Skewness skewness	0.083
峰度 Kurtosis kurtosis	-0.575
角度范围 angular_range	— – 0.2400 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	74860000.0000
实空间数据点数 n_real_points	49
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.940; Stabil: 1.000; Sysdev: 0.049; Positv: 1.000; Valcen: 0.983; Smooth: 0.971

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (3)

Entity 1 polymer

描述 description	Guard cell S-type anion channel SLAC1,Green fluorescent protein
分子量 formula_weight	94073.0 kDa
突变 mutation	S59D,T62D,S65D,S86D,S107D,S124D,S146D,S152D,S595R,Y604N,F629L,S630T,Q645R,F664S,N670T,Y710F,M718T,V728A,I736V,A771V
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C
原子数 atom_count	9285

MERKQSNAHSTFADINEVEDEAEQELQQQENNNNKRFSGNRGPNRGKQRPFRGFSRQVDLEDGFDVLNRESRERDDKKSLPRSGRDFGGFESGGIINGGDGRKTDFDMFRTKSTLSKQKSLLPDIIRERDIENSLRTEDGETKDDDINENVDAGRYFAALRGPELDEVKDNEDILLPKEEQWPFLLRFPIGCFGICLGLSSQAVLWLALAKSPATNFLHITPLINLVVWLFSLVVLVSVSFTYILKCIFYFEAVKREYFHPVRVNFFFAPWVVCMFLAISVPPMFSPNRKYLHPAIWCVFMGPYFFLELKIYGQWLSGGKRRLCKVANPSSHLSVVGNFVGAILASKVGWDEVAKFLWAVGFAHYLVVFVTLYQRLPTSEALPKELHPVYSMFIAAPSAASIAWNTIYGQFDGCSRTCFFIALFLYISLVARINFFTGFKFSVAWWSYTFPMTTASVATIKYAEAVPGYPSRALALTLSFISTAMVCVLFVSTLLHAFVWQTLFPNDLAIAITKRKLTREKKPFKRAYDLKRWTKQALAKKISAEKDFEAEEESHHGSENLYFQSMSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKYPYDVPDYAGGGSHHHHHHHHHH

来源 Source

来源生物 organism	Aequorea victoria
通用名 common_name	Water jellyfish, Mesonema victoria
NCBI 分类编号 ncbi_taxonomy_id	6100
宿主生物 host_organism	Spodoptera frugiperda

Entity 2 non-polymer

描述 description	CHLORIDE ION
分子量 formula_weight	35.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

Entity 3 non-polymer

描述 description	CHOLESTEROL HEMISUCCINATE
分子量 formula_weight	486.7 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	triclinic

分辨率 resolution	2.70 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	181365

fit	/app/data/saxs_computation/j0/8j0j/8j0j.fit
pddf	/app/data/pr_computation/j0/8j0j/8j0j.pr.out
profile	/app/data/saxs_computation/j0/8j0j/8j0j.dat