8ouw

Cryo-EM structure of CMG helicase bound to TIM-1/TIPN-1 and homodimeric DNSN-1 on fork DNA (Caenorhabditis elegans)

Method: ELECTRON MICROSCOPY D_max: 228.4 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8ouw
沉积日期 deposition_date	2023-04-25
结构标题 title	Cryo-EM structure of CMG helicase bound to TIM-1/TIPN-1 and homodimeric DNSN-1 on fork DNA (Caenorhabditis elegans)
关键词 keywords	DNA Replication, DNA Replication Initiation, Replisome, DONSON, Replication; REPLICATION
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	68.15 Å
回转半径 Rg (电子) rg_electron	67.75 Å
零角强度 I(0) i0	8663150000.00
分子量 molecular_weight	767890.0 kDa
排除体积 excluded_volume	953410 Å³
包络体积 envelope_volume	1570000 Å³
水化壳体积 shell_volume	187830 Å³
包络直径 envelope_diameter	234.4 Å
壳层 Rg shell_rg	72.78 Å
包络 Rg envelope_rg	65.64 Å
形状 Rg shape_rg	67.78 Å
总 Rg total_rg	67.74 Å
总原子数 total_atoms	53754
残基数 n_residues	6631
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	228.4 Å
Rg (实空间) rg_real	67.94 Å
Rg 误差 (实空间) rg_real_error	2.20 Å
I(0) (实空间) i0_real	8.6630e+09
I(0) 误差 (实空间) i0_real_error	2.0070e+08
Rg (倒空间) rg_reciprocal	68.76 Å
I(0) (倒空间) i0_reciprocal	8676000000.0000
解质量估计 total_estimate	0.8800
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	79.4 Å
偏度 Skewness skewness	0.124
峰度 Kurtosis kurtosis	-0.561
角度范围 angular_range	— – 0.1150 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	701600000.0000
实空间数据点数 n_real_points	24
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.858; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.976; Smooth: 0.886

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (19)

Entity 1 polymer

描述 description	DNA replication licensing factor MCM2
分子量 formula_weight	99448.6 kDa
EC 编号 ec_number	3.6.4.12
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2
原子数 atom_count	53754

MADRANNDDDVDRQPLPIADDADDDVDGIDEMFNNEDEDPEDEEGENLFGDDMERDYREQPELDQYSESGMDDASDVGSLSVSARRAAEREMAQRDQLLDDDALMYEDGDSEEVDTRRRGRGRRGRGDAADDDSVPMEEEDIPVDILENIRGRTIRDHVSDEAVAKEIERRFKNFLRSFHEPGNKQTKYIQMIKSMAADNRESLEVSFTDLSDDNGEQNISYFLPEAPNEMLAIMDRAATEVVMNMYPFYSRVCNEIKVRISQLPVEEDIRMLRQVHLNMLIRTAGVVTIASGILPQLAVVKYDCVACGYLLGPFVQQNDEEVRPTICPSCQGKGPFELNVENTVYHNYQRITMQESPNKVAAGRLPRSKDVILLGDLCDSCKPGDEIEVTGVYTNNFDGSLNYKQGFPVFNTLIHANHITNKDKMASDQLTDEDIKAIRELSQDPNISQRVFSSIAPSIYGHDDVKRAIALALFRGEAKNPGAKHRLRGDINVLLCGDPGTAKSQFLRYAAHIAPRSVLTTGQGASAVGLTAYVQRHPVTREWTLEAGAMVLADKGVCLIDEFDKMSDQDRTSIHEAMEQQSISISKAGIVTSLHARCTVIAASNPIGGRYNPTRTFAENVDLTEPILSRFDVLCVIRDSVDSVEDERLAKFVVGNHRTHHPDAKKIVKEGDELEEDQMDERTGVRLIPQDLLRKYIIYAREKCHPTLPEQHSEKFSNIFAQMRKESMATGSVAITVRHVESMIRLSEAHAKLHLRSYVNDEDCAAAIRVMLESFVNTQKASIMRMMKKTFSRHLTENRSANELLLFILKQLIRQQMHYATARAAAGTILQSVTIPESEFIEKAQQLRIENVKPFYTSEIFASNNFLYDPSKKTIVQEIF

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 2 polymer

描述 description	DNA replication licensing factor MCM3
分子量 formula_weight	90810.7 kDa
EC 编号 ec_number	3.6.4.12
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3

MDGQADGMFIDRAEDARKQEITEQYLNFLDNQADTFHYRQKIVKMMNDGESRLVMNLDEIREILPERAEGLLTQSVLEMNCLQSALQMAINRSDVQAVVKTGFHVGFEGTFGERHVNPRTLKSSYLGNLVCCEGIVTKCSTVRQKLLTSVHYCPATNKVLEKKFADFTMLDTAVTNNNYPTEDENKNPLETEFGHSVYKDHQTFTIQELPESAPAGQLPRAVDCVADLDLADRVKPGDRVRIIGVFRVLPNKQNGVSSGSFRSIIIINHIQMLSKEVIPNFEPQDVKDVRKISKSRNPFELLARSLAPSICGHEETKKALLCLLLGGMEKILNNGSRLRGDINVLLIGDPSVAKSQLLRYVLRMAPRAITTTGRGSSGVGLTAAVTTDPDSGERRLEAGAMVLADRGVVCIDEFDKMSDIDRTAIHEVMEQGRVTISKAGIHAKLNARCSVLAAANPVYGRYNPFKSPMENIGMQDSLLSRFDLIFVLLDEHDADKDANVAEHVLKLHTYRTQGEADGTVLPMGGGVETISTINMETKKASSSIYEENTQWAGIQDTKILTMDFMRKYIHLARSVQPKLTDEATEYISEVYADIRSFDITKTDQERTMPVTARQLETLIRLSTAIAKARFSKKVEREDTEKAYNLLHFACFKEKPKARQEYEKKKRAPRIDGDDDEDQSDNEDMATSEEPQSSSVPTAATPRRGVRRRAEDDDSQDSMMDTTIGESQPTAKKARTGPAAIGVDRYKELRKYVRKAFDDIGSTDDMVELQIITDSVQTQAGSHKFTEDELKAGYEQLENDNAAMIAEDKITLI

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 3 polymer

描述 description	DNA replication licensing factor mcm-4
分子量 formula_weight	91687.6 kDa
EC 编号 ec_number	3.6.4.12
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	4

MPRSNDPAEPQSESADLFAEPSSQHSSMRSGSEQVAGNMSPRSTSSASALQYGSEMGSVSQMSSASTLRRGGPRGDLGIAAVDHRTVQIRGMEDEMAGDDGQPRLYVWGTRICVADVQRSFRDFLTTFKISQLAEDENMMTGHDEALHEIDVNHPYYMERLLECNDAEVTHINLNLKHLNAFSEALYRKVIAYPADVIPYLDIVVNEVFAERFNRTLAQSIELRPFNAQKTRNMRGLNPNDVDQLITISGMVTRTSSLIPEMRSGYFQCAVCAFGIESEVDKGRIEEPVVCTNCSNTHCFQLVHNRSVFLDKQVVKLQESPDDMPSGETPHTVSVYAHGSLVESVQPGDRITVTGIFRATGMKVNPKQRALASVYRTSIDALHFRKMDTSRLHQDNGETITEERIQQIIELSKRPDIMDALAQSIAPSIYEHDDVKRGLLCLLFGGTRKDDETTNKTKLRSEINILLCGDPGTSKSQMLQYVYRLLPRSQYTSGKGSSAVGLTASVSRDADTKQLVLQTGALVLADNGVCCIDEFDKMNESARSVLHEVMEQQTLSIAKAGIICQLNARASVLAAANPVDSKWNRNKTIVENITLPHTLLSRFDLIFLIVDAQDEMQDRRLGNHLVSLYFENDGNQEKTEHVDMNLLRDYIAYAKANIHPKLSEEASQFIIEKYLFMRKAGAQHGQITAYPRQLESLIRLSEAHAKIRLSQEVSVDDVEKAFTLWREALKQSAVDPSTGRVDVAILASGMSASGRKAVEAMCEAVLKQLKTAKGFVTSKALFHTLKSADKTCQREVFDEAINELSKKESIARSGDRIRFQTEA

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 4 polymer

描述 description	DNA replication licensing factor mcm-5
分子量 formula_weight	85057.7 kDa
EC 编号 ec_number	3.6.4.12
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	5

MSNLDNPGIYYQERFFANDGVPDTGRELIAEYRQLITQFRNFIRDFSTGGFGMIYRDQLKRNYFSHEYRLEINLNHLKNFDEDIEMKLRKFPGKVLPALEEAAKIVADEITTPRPKGEEKLHDIQVTLTLDEYPTSLRQVKSAQVSQVVKISGIIVAAAQVRSKATKVTLQCRQCKHTIPDVSIKPGLEGFALPRTCAAPQQGQMQRCPIDPYIMLPDKCECVDYQTLKLQENPEDVPHGEMPRHLQLFTERYLTDKVVPGNRVTIVGVYSIKKLIQKKGGDKSLQGIRTPYLRVLGIHMETSGPGRTNFTTFTPEEERMFKTLAQRKDAYELIAKSIAPSIYGSADIKKSIACLLFGGARKKLPDGITRRGDINVLLLGDPGTAKSQLLKFVEQVSPIGVYTSGKGSSAAGLTASVIRDPQSRSFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVYGRWDESRGDDNIDFMPTILSRFDMIYIVKDTHDVLKDATLAKHVIEVHVNASAAKERDIAGVPKTATTDSDGVMTMFDTDGFLTIEFLKKFVTYARLNCGPRLTPQASEKLVNHYVKMRNPVVNADAFKSGKKAHNSAIPITVRQLEAIVRIAESIAKMELQQFATDKHVEEALRLFRVSTIEAAATGNLAGVEGFTSTADQEALNRIEVQMKKRFAIGTHVSEHLIVQDFVARQHYRESLVKKVIDNLVRRGDLQQKMQRKMLYRVR

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 5 polymer

描述 description	DNA replication licensing factor mcm-6
分子量 formula_weight	91248.3 kDa
EC 编号 ec_number	3.6.4.12
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	6

MDNIIGGQAQQVEDTDGTRVQNEFSKFLKSFKDQKNEFIYKSAMKELVQPEKNTIFINMQHLYKFSNNLATTIELQYYRVYPFMCEALHLATLDACDESERQQMFKKQLYVSLFNLDAKTKVRELSADKVGGLVRIAGQIVRTHPVHPELSRACFVCEDCGVTTRDVQQQFRYTQPTKCANPQCMNRTRFSLDVNSSTFVDFQKIRIQETQAELPRGSIPRTVDVIVRGEMVETVQPGDKCDIVGTLIVIPDIAQLSTPGLRAETSNQNRGRATDKSEGITGLKALGVRDLTYKMAFLACHIQQTESLVGGDASGAVEETDYLDLWSKMSTEDRATLKKMSDDKKIEKNIVDSLFPNIYGNHEVKLGVLLMLLGGVAKKSRDEGTSLRGDINVCLVGDPSTAKSQVLKAVEEFSPRAIYTSGKASSAAGLTAAVVKDEESFEFVIEAGALMLADNGVCCIDEFDKMDLKDQVAIHEAMEQQTISITKAGVKATLNARASILAAANPVNGRYDRSRPLKYNVQMSAPIMSRFDLFFVLVDECNEVTDYAIARRILDNHRAISEHTERDSVYKIDDIKKYIAFARCFKPKISDKAAETLVREYKKLRMSDSNNAATSSWRITVRQLESLVRLSEALARLHCGKEVLVEHVEKAAELLNKSIVRVEQPDIALDDDDFDNNIMVVEADKENQRGDDSMDHDGEKENAPKIDIAKLKISFKEYKQLSDVLVLHMRSDEDNQGEDEYDGVKQSALVEWYLSTIEADLETEEDFNVQKTICERVIHRLIHQDHVLLEVEQGEDPTLCVHPNYVIADE

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 6 polymer

描述 description	DNA replication licensing factor MCM7
分子量 formula_weight	81721.4 kDa
EC 编号 ec_number	3.6.4.12
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	7

MKTTTYNTDWAAEKTKIRSFFDEYYVDNEDGSGKAFPYRDQVFEIARRDKQAIVVNVDHIKESDIPDALELSEAITSNTKRYEVLFKDTISDMIQDYLGDKQAPVIDALDAYMFQRLHMDRNEGAANEEVSLQDKRKKYPPQLLQRFEVYFTTDDAAHETCVRNIKATEIGHLVSMKGVVIRATEVKPCVEVMTYTCDTCAAEVYQPVKGMQFTPPVNCPNKECVEAKANGRLHMQLRGSKFVKFQELKIQELSEQVPVGSIPRTMTVHVYGEMTRKCNTGNVVHVSGVFLPIMQSGFRPTGGLVADTYLEAHYINNLDDNPTFNGVQSAELEVLRRKGDNYETLAASIAPEIFGHVDVKKCLLMALVGGNDNSSNGMKIRGCINVLMMGDPGVAKSQLLGYVNRLAPRSQYTTGRGSSGVGLTAAVMKDPVTGEMSLEGGALVLADGGICCIDEFDKMMDHDRTAIHEVMEQQTISIAKAGIMTTLNARTAIIAAANPAYGRYNPNRSIEQNVDLPAALLSRFDLILLMQDKADRENDKILAEHITYVHQHGCHPNREKKDLISLETLREYISLCKTYTPTVDPALRERIVEAYVEMRRDARYSSDPTFVSPRMILGIVRMATARAKLRLSTIVDESDVEEALRLMQFAKDSLRPEQNKIEKRMAPVDAAFAVLRELYHADNAPIAISNAIQRCARKGISEVALKKCLDQYTANGLLVMDRQNIVFAMN

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 7 polymer

描述 description	Probable DNA replication complex GINS protein PSF1
分子量 formula_weight	23064.4 kDa
突变 mutation	Protease-cleaved N-terminal expression tag (Gly-Pro-Gly-Ser)
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A

GPGSMSSGDQNRGGVADKALQLVLEMKRNPDVLPPYNTELVRQCYQKIDELFQKNAAVVEKIRAGLPHDSTLLQPRLAAMCHIRRCMMAYVNERKNRIRSFRWKYGGALPASVRNALCDAEIQFFNEYSSTLARFQSNLGEGGVNLLLHSAPPKSLFVQVRALEDYGEFETSDGTQVQLSKDSLHSLPRQDCEMLIRQGVLELVH

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 8 polymer

描述 description	Probable DNA replication complex GINS protein PSF2
分子量 formula_weight	20349.3 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

MNAERCEFIAGNSLIEVIPSISDDRPIHLISGDIGPFEAGVPCRIPVWTAILMKRKHNCKVVAPQWMDVDELKKILTSETESQGLAKLPDHFFEISHMLVRDAREDIFEVEAVKSLVQDIYDRRDAKLRSSAIEFLRQNQTCHAQLDNVQLIEASSARATLEACRQMGAVVRNKHESTPL

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 9 polymer

描述 description	DNA replication complex GINS protein PSF3
分子量 formula_weight	21717.7 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MAGFEIQRVESKFFDLDDIIAKSDSTSCTFEIGDLNPDFFQEMLGVSKPTQNADGYGVDAPYWLLESVRSSFSIQLPKAYSVNMQNVLNADSKKLNLSGLQQHFYGNGMQLCRLMKGENPDGALSLARCLVSTLTQRLGEIVSTATHLQSKGEKFDSLETKVFLEGKRCKEDIDTWLRQDNKCSSKKRKRLSL

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 10 polymer

描述 description	DNA replication complex GINS protein SLD5
分子量 formula_weight	25705.1 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MAVTDSATTFLDFDDDEYDEMTTPEEVLRKMTATWQNELCAPCLLPTQMELVEILLDQIQGMEENIGKQTDKMQLRISVHRVELQRIGFITSDYVRCRLQKIESNPHDAIDQHKKRKEEGKSDLLSESEMKFAEEYALAESNLFQKTVLEFMPAALKKMPVPRGDHDDVMVYAKVTSDDVGNVAIPDWQDLNGEVILEMEPESCHLIPFESVHQLVEDGNIQLM

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 11 polymer

描述 description	Cell division control protein 45 homolog
分子量 formula_weight	66245.8 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MIIEDNFRQEFYNKIKKGDKSVVLIVSVDTDALCTTMILTHLLRCDDIPFSILAVEGWSSVEKIFADSQEQKCHYILINCGATRSLTRLQIPPASIAFVIDSHRPFHIENVYENGQIHLLANPSEMSELQIPDLESVIREDSDDEEDSDEDEDQEYISYEQRMEKIRRKAIKREEMQIWERQRRTILWRYYESTWFSSPSCVTLLEMAAEMNRVSAETMWFTAVGLNSAMADKLISIEMYTQICVDRMRPFVHRFMPKNIVNQGKVDDLLHITFGRELPLALYSHWDLFSAMMVSEYFSIKTKNWTQKGDVNIRHLLAQLGITLHETKQKFEALPTEQRNLVVDVLEKEMDSSFATFFGTLGYCGKLSACDVARAVTLRLEMPKSETIMNRFRSGQSILRSSITGERQDRLNLNNTFTSICQRTLQVSWKTVAAAINQSEIIPNGPYYLFSCSRSIDEDMVDSRHFLYNTAGFMIRAFASMKKGRTTKPLIAMFPLTGESAGWLVVTGVMPIATIYEDSLLKTCIGRAFERVKKMTPPLRIVDDYFNSDIIRLKSEDRTRFIDFLQTVFEGTSN

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 12 polymer

描述 description	Downstream Neighbor of SoN homolog
分子量 formula_weight	66079.4 kDa
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F,G,H

MSDEHYNPRNRLKTQIKRRSSCRNILNAVPPPPPTDFEGLEGFTDSCPAPTANNPFKVSSPQKKRQKPQVTFSQDDPFAASISRSPDPLKTPTKGVRSSPRKRKSPQKFERFQSFDPALLQEFHPVDFSALLATSSSAQKPAEPAQESAEDLPTDLRIGSKMRIVSKVSFPWMNTRKSTGNVIVKFQTNDRFDGMQYFNKTFLNGYEDPTVPLPVSPMSILEAASLFYQFPVIPGLKMYPRITAEQENVSRVSLAPLVTSTMLEQWQECYAELYSSYKKGQRGSFYVATAVCNVLFTKMPLSGGGDDVAADDTSQSCSQAFRGQKLVAVITHTTSAIREHLRSQGVDFEAPKPTSSLKRVSSSPQLHHDQSTLDCLLASESKENSQMGPTTFGGIENSENSDESPLKKDQDSDENESPMKLANQWLNEIGVSPRTVKNTVSRELSSQVTDRAGFQCLVVHGSKIHALFNALMDSDVVHEKTGPYTKIPPTLIATSPFLYGQLLSLTKSSNIFAKAGTKCTEYELKLENGPVLPHCAKMMTQFIRSTNMCSTEEKPVTMQVMDRHTSQGMSDWTETATNWNTVTIAENCVKWTKN

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 13 polymer

描述 description	DNA Leading Strand Template
分子量 formula_weight	26396.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polydeoxyribonucleotide
链编号 strand_id	I,J

TAGAGTAGGAAGTGATGGTAAGTGATTAGAGAATTGGAGAGTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT

Entity 14 polymer

描述 description	Protein timeless homolog
分子量 formula_weight	157256.7 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K

MNVLVQGAVHALGYYEDGKYSREPDCYESIRDLIRYLREDGDDHTARIECGRHNLVEQDLVPMVKCEDLTDDEFDIAIRLMVNLCQPAISTMRGKPPADRDQWKMYWELEENLRRAKTAFSDAHFFTAIKKRIDNYFIDTEYEDRDERLRLVVERIVLLIKYVFSINPDTSEGRRTRIEDSSHDRVIIAFLESGIDKTLMHIANQPREKEFHVTILDIFALILKEQTAEDLATKSEEVSTAEQKKTEEEFRKIIENHVVKETQKRKSFSRFGGSYTIKGLKGISANSSQVVFKPIQNVEKHNFLDDRKAKKRAPRNRRPFEIDTNSHFASSEVRGMLRDMVIRIIETCFNRLMKSSKTTVFVQVQKTSQINYFFLIKFVLRFVRLSRQDHLLERISECIGVEAFHENNVQLTEYVENATTLKGVEAKSHGLKAQYALGAYNELVLLHRYIYEHAKEENERKFAKRALEHIVNVEEYRELPIFIIKKFSSSVLSNNFLRELVLTTHHYMKLVERFVKTGALKKVTKKVKVRKATKKSKMSEEDVRSEFDGMSKKDLDRLWEESKGLVLQILKKEVPEMRGMNPIDSQLDVPVDAQQKFAKLSIQRSLRSRGFPAAVGLYHASRALWPESFKRGLTDFQDSPGEEDQLQELEQLLKADMKKVAKDLKKAESCKTCDEDPAYKKYDKMDATALQSLWEQSTDTLARILSHELPESESTSPVNWQLDITPDVQQKFAMLAIQRALRARDLPAAVGLYHTSRKLWPGDEAIFGAPGIGVEEEIAELKAILEADLHEVAREMKVAEDRAEDPDEEDPAEPYDSEQEEEEEVPAWKVEEIDFQFDSYVCKFSNVDVLKWYVFLLNDFSKNSTELNQALVKMLHRIAFDLKLPIKLYQVSLFQVFSKVNEHFTHLSKDLRKSSRLYELYQFGFHLLKKFFSKFTGDLAIEALFWKGPRECFEIENGYGSWVKSREADIRVWTEDLEIELRNLYEEYRTMETRDGIDVLDFIEHNLSRARSRKKVAKKLIEFGFDLLGAKWKNSDKARMDSVLPIGDIQKWYDEWKEAGARGDLVNVLQEKLNEDLGMEISRKKILKQLAHMDILYEKPKKEKPLPQWDTGLIEELKKLKEQYDDIPDALNMLGVNIVRYVMKRLSEKKPTRQVERHLESLGATIPERSKKSEKNGKKFDDFLNDDDDDSENDVGGGSEDDEEEEIVMKSKRIIPDSEDEEEHIEQEEAQKKLEKVAEKPNTLMGMIAGRKRKLAQLESDSSDESDDDDSAEKEEKKLPAAEDDSDLEEDAVIYKRSYVDALLTGGSIAGNGITETRRDTSEEREDDDDEDPFTKKLTFKRRIVMSDNEDEA

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 15 polymer

描述 description	Protein TIPIN homolog
分子量 formula_weight	27415.2 kDa
突变 mutation	Protease-cleaved N-terminal expression tag (Gly-Pro-Gly-Ser)
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

GPGSMDEMEDFFENDELDREPSPMGDEAIEDNSGEGGTRRVVEPKLLRTKRLANPRLALNERILTGPKGISALRETLKDFKPNPKDDPYANLEKLMKKYAYWGHLMFPKMKTEDVLNRVETLGTRRQVKVFMIKHRLGETGEDSEHEEQENQKNGIIDDGASDNDDDLFKDLPEKEVTTEKAKNSEKSDQKTAEIDENVEEEYRMMEEERLREEQEAKEAADEDALMEDFGDMNNDW

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Saccharomyces cerevisiae

Entity 16 polymer

描述 description	DNA Lagging Strand Template
分子量 formula_weight	18524.9 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polydeoxyribonucleotide
链编号 strand_id	M

GGCAGGCAGGCAGGCACACACTCTCCAATTCTCTAATCACTTACCATCACTTCCTACTCTA

Entity 17 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	5

Entity 18 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

Entity 19 non-polymer

描述 description	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
分子量 formula_weight	506.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.75 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	33900

fit	/app/data/saxs_computation/ou/8ouw/8ouw.fit
pddf	/app/data/pr_computation/ou/8ouw/8ouw.pr.out
profile	/app/data/saxs_computation/ou/8ouw/8ouw.dat

Cryo-EM structure of CMG helicase bound to TIM-1/TIPN-1 and homodimeric DNSN-1 on fork DNA (Caenorhabditis elegans)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (19)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 polymer

聚合体 Polymer

来源 Source

Entity 5 polymer

聚合体 Polymer

来源 Source

Entity 6 polymer

聚合体 Polymer

来源 Source

Entity 7 polymer

聚合体 Polymer

来源 Source

Entity 8 polymer

聚合体 Polymer

来源 Source

Entity 9 polymer

聚合体 Polymer

来源 Source

Entity 10 polymer

聚合体 Polymer

来源 Source

Entity 11 polymer

聚合体 Polymer

来源 Source

Entity 12 polymer

聚合体 Polymer

来源 Source

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

来源 Source

Entity 15 polymer

聚合体 Polymer

来源 Source

Entity 16 polymer

聚合体 Polymer

Entity 17 non-polymer

Entity 18 non-polymer

Entity 19 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)