8rc5

Complex between the RecA-like Sak4 SSAP and the SaPI2 Stl master regulator

Method: ELECTRON MICROSCOPY D_max: 210.1 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8rc5
沉积日期 deposition_date	2023-12-06
结构标题 title	Complex between the RecA-like Sak4 SSAP and the SaPI2 Stl master regulator
关键词 keywords	;Annealase, SSAP, Single Strand Annealing, Single Strand Binding, Recombineering, Recombination, SaPI, Bacteriophage, Staphylococcal, Complex, SaPI induction, SaPI2, Mobile Genetic Element, MGE, PICI, Phage-Inducible Chromosomal Island, C18, Ring ;; RECOMBINATION
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	86.50 Å
回转半径 Rg (电子) rg_electron	85.68 Å
零角强度 I(0) i0	11430400000.00
分子量 molecular_weight	914280.0 kDa
排除体积 excluded_volume	1146300 Å³
包络体积 envelope_volume	2500400 Å³
水化壳体积 shell_volume	246640 Å³
包络直径 envelope_diameter	268.3 Å
壳层 Rg shell_rg	92.51 Å
包络 Rg envelope_rg	76.47 Å
形状 Rg shape_rg	85.70 Å
总 Rg total_rg	85.70 Å
总原子数 total_atoms	64360
残基数 n_residues	7866
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	210.1 Å
Rg (实空间) rg_real	84.47 Å
Rg 误差 (实空间) rg_real_error	0.36 Å
I(0) (实空间) i0_real	1.1130e+10
I(0) 误差 (实空间) i0_real_error	2.1500e+08
Rg (倒空间) rg_reciprocal	88.35 Å
I(0) (倒空间) i0_reciprocal	11490000000.0000
解质量估计 total_estimate	0.6126
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	116.7 Å
偏度 Skewness skewness	-0.096
峰度 Kurtosis kurtosis	-0.531
角度范围 angular_range	— – 0.0900 Å⁻¹
当前正则化参数 α current_alpha	0.3048
最高正则化参数 α highest_alpha	406200000.0000
实空间数据点数 n_real_points	19
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.963; Stabil: 0.984; Sysdev: 0.000; Positv: 1.000; Valcen: 0.594; Smooth: 0.001

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (3)

Entity 1 polymer

描述 description	ORF016
分子量 formula_weight	31653.7 kDa
来源方法 src_method	man
拷贝数 entity_count	18

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1A,4A,1B,4B,1C,4C,1D,4D,1E,4E,1F,4F,1G,4G,1H,4H,1I,4I
原子数 atom_count	64360

MGSSHHHHHHSSGLVPRGSHMTEKTNQDVDILTQLGVKDISKQNANKFYKFAIYGKFGTGKTTFLTKDNNALVLDINEDGTTVTEDGAVVQIKNYKHFSAVIKMLPKIIEQLRENGKQIDVVVIETIQKLRDITMDDIMDGKSKKPTFNDWGECATRIVSIYRYISKLQEHYQFHLAISGHEGINKDKDDEGSTINPTITIEAQDQIKKAVISQSDVLARMTIEEHEQDGEKTYQYVLNAEPSNLFETKIRHSSNIKINNKRFINPSINDVVQAIRNGN

来源 Source

来源生物 organism	Staphylococcus phage 52A
NCBI 分类编号 ncbi_taxonomy_id	2908114
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

描述 description	Helix-turn-helix XRE family protein
分子量 formula_weight	27189.6 kDa
来源方法 src_method	man
拷贝数 entity_count	18

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2A,3A,2B,3B,2C,3C,2D,3D,2E,3E,2F,3F,2G,3G,2H,3H,2I,3I

MIRNRLSELLSERGLKISRVAKDVKIARSSLTSMAQNDSEMIRYDAIDKLCSYLHISPSEFFEHNPINFDFTFDEEPNYKINDVFEGFEVTANITHAFSIENFDFEILVDVELDNRQKLNFDLDVSYKETEKITNSQHRFIFTIKNEDENIGLKKYVDSLSAGLKNLLFKKINQKLSGYVSEIIVKNIDDIEELFPNKGEKSTTLHKEILQTDSRLSSDIFKEYLEHHHHHH

来源 Source

来源生物 organism	Staphylococcus aureus
NCBI 分类编号 ncbi_taxonomy_id	1280
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 3 non-polymer

描述 description	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
分子量 formula_weight	523.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	16

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7. 引用文献 (1)

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分辨率 resolution	3.35 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	141863

fit	/app/data/saxs_computation/rc/8rc5/8rc5.fit
pddf	/app/data/pr_computation/rc/8rc5/8rc5.pr.out
profile	/app/data/saxs_computation/rc/8rc5/8rc5.dat

Complex between the RecA-like Sak4 SSAP and the SaPI2 Stl master regulator

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (3)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)