8rdj

Plastid-encoded RNA polymerase transcription elongation complex (Integrated model)

Method: ELECTRON MICROSCOPY D_max: 277.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8rdj
沉积日期 deposition_date	2023-12-08
结构标题 title	Plastid-encoded RNA polymerase transcription elongation complex (Integrated model)
关键词 keywords	Transcription, chloroplast, RNA polymerase, photosynthesis, GENE REGULATION; GENE REGULATION
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	76.13 Å
回转半径 Rg (电子) rg_electron	76.39 Å
零角强度 I(0) i0	13919700000.00
分子量 molecular_weight	994760.0 kDa
排除体积 excluded_volume	1241300 Å³
包络体积 envelope_volume	1964900 Å³
水化壳体积 shell_volume	210880 Å³
包络直径 envelope_diameter	264.1 Å
壳层 Rg shell_rg	77.00 Å
包络 Rg envelope_rg	75.08 Å
形状 Rg shape_rg	76.42 Å
总 Rg total_rg	76.29 Å
总原子数 total_atoms	69954
残基数 n_residues	8540
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	277.8 Å
Rg (实空间) rg_real	80.01 Å
Rg 误差 (实空间) rg_real_error	1.82 Å
I(0) (实空间) i0_real	1.3990e+10
I(0) 误差 (实空间) i0_real_error	3.2090e+08
Rg (倒空间) rg_reciprocal	76.31 Å
I(0) (倒空间) i0_reciprocal	13930000000.0000
解质量估计 total_estimate	0.8885
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	92.6 Å
偏度 Skewness skewness	0.547
峰度 Kurtosis kurtosis	0.172
角度范围 angular_range	— – 0.1050 Å⁻¹
当前正则化参数 α current_alpha	0.9996
最高正则化参数 α highest_alpha	869600000.0000
实空间数据点数 n_real_points	22
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.770; Stabil: 0.861; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.713

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (27)

Entity 1 polymer

描述 description	DNA-directed RNA polymerase subunit alpha
分子量 formula_weight	37945.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	70206

MVREKVKVSTRTLQWKCVESRRDSKRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRAKFENIPHDYSNIVGIQESVHEILMNLNEIVLKSNLYGTRNALICVQGPGYITARDIILPPSVEIVDNTQHIATLTEPINLCIGLKIERNRGYSLKMSNNFEDRSYPIDAVFMPVQNANHSIHSYGNGNEKQEILFIEIWTNGSLTPKEALHEASRNLINLFIPFLHVEEETFYLENNQHQVTLPLFPFHNKLVNLRQKKKELAFQYIFIDQLELPPRIYNCLKKSNIHTLLDLLNNSQEDLIKMEHFHIEDVKKLLDILEKK

Entity 2 polymer

描述 description	DNA-directed RNA polymerase subunit beta
分子量 formula_weight	121209.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MLGDGKEGTSTIPGFNQIQFEGFYRFIDQGLIEELSKFPKIEDIDHEIEFQLFVETYQLVEPLIKERDAVYESLTYSSELYVSAGLIWKTNRNMQEQRIFIGNIPLMNSLGTFIVNGIYRVVINQILQSPGIYYQSELDHNGISVYTGTIISDWGGRLELEIDKKARIWARVSRKQKISILVLSSAMGSNLREILENVCYPEIFLSFLTDKEKKKIGSKENAILEFYQQFSCVGGDPIFSESLCKELQKKFFHQRCELGRIGRRNINWRLNLNIPQNNIFLLPRDILAAADHLIGMKFGMGTLDDMNHLKNKRIRSVADLLQDQLGLALARLENVVKGTIGGAIRHKLIPTPQNLVTSTPLTTTYESFFGLHPLSQVLDRTNPLTQIVHGRKLSYLGPGGLTGRTANFRIRDIHPSHYGRICPIDTSEGINVGLIGSLSIHARIGDWGSLESPFYELVEKSKKAQIRMLFLSPSQDEYYMIAAGNSLALNRGIQEEQVVPARYRQEFLTIAWEEVHLRSIFPFQYFSIGASLIPFIEHNDANRALMSSNMQRQAVPLSRSEKCIVGTGLERQVALDSGVPAIAEHEGKILYTDTEKIILSGNENTLSIPLIMYQRSNKNTCMHQKPQVRRGKCIKKGQILADGAATVGGELALGKNVLVAYMPWEGYNFEDAVLISECLVYGDIYTSFHIRKYEIQTHVTTQGPERITKEIPHLEGRLLRNLDKNGIVMLGSWVETGDILVGKLTPQVAKESSYAPEDRLLRAILGIQVSTSKETCLKLPIGGRGRVIDVRWVQKKGGSSYNPEIIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGRPVDMVFNPLGVPSRMNVGQIFECSLGLAGSLLDRHYRIAPFDERYEQEASRKLVFSELYEASKQTANPWVFEPEYPGKSRIFDGRTGDPFEQPVIIGKPYILKLIHQVDDKIHGRSSGHYALVTQQPLRGRSKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDAPESFRLLVRELRSLALELNHFLVSEKNFQINRKEV

Entity 3 polymer

描述 description	;DNA-directed RNA polymerase subunit beta' ;
分子量 formula_weight	78761.9 kDa
EC 编号 ec_number	2.7.7.6
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MIDRYKHQQLRIGLVSPQQISAWATKKIPNGEIVGEVTKPYTFHYKTNKPEKDGLFCERIFGPIKSGICACGNYRVIGDEKEDPKFCEQCGVEFVDSRIRRYQMGYIKLTCPVTHVWYLKRLPSYIANLLDKPLKELEGLVYCDFSFARPITKKPTFLRLRGSFEYEIQSWKYSIPLFFTTQGFEIFRNREISTGAGAIREQLADLDLRIIIENSLVEWKQLGEEGPTGNEWEDRKIVRRKDFLVRRMELAKHFIRTNIEPEWMVLCLLPVLPPELRPIIQIEGGKLMSSDINELYRRVIYRNNTLTDLLTTSRSTPGELVMCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIVVGPSLSLHRCGLPREIAIELFQTFVIRGLIRQHLASNIGVAKSQIREKKPIVWEILQEVMQGHPVLLNRAPTLHRLGIQSFQPILVEGRTICLHPLVCKGFNADFDGDQMAVHVPLSLEAQAEARLLMFSHMNLLSPAIGDPISVPTQDMLIGLYVLTSGTRRGICANRYNPCNRKNYQNERIYETNYKYMKEPFFCNSYDAIGAYRQKRINLDSPLWLRWQLDQRVIASKEVPIEVHYESFGNYHEIYAHYLIVRSVKKETLYIYIRTTVGHISFYREIEEAIQGFSQACSYDT

Entity 4 polymer

描述 description	;DNA-directed RNA polymerase subunit beta'' ;
分子量 formula_weight	156388.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MAERANLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQVKTLGFQQATATSISLGIDDLLTIPSKGWLVQDAEQQSLILEKHHHYGNVHAVEKLRQSIEIWYATSEYLRQEMNPNFRMTDPFNPVHMMSFSGARGNASQVHQLVGMRGLMSDPQGQMIDLPIQSNLREGLSLTEYIISCYGARKGVVDTAVRTSDAGYLTRRLVEVVQHIVVRRTDCGTIRGISVSPRNKSRMMSERIFIQTLIGRVLADDIYIGSRCVAFRNQDLGIGLVNRFITFGTQSISIRTPFTCRSTSWICRLCYGRSPTHGDLVELGEAVGIIAGQSIGEPGTQLTLRTFHTGGVFTGGTAEHVRAPYNGKIKFNEDLVHPTRTRHGHPAFLCYIDLSVIIESEDIIHSVTIPPKSFLLVQNDQYVESEQVIAEIREGTYTFHFKERVRKYIYSDSEGEMHWSTDVSHAPEFTYSNVHLLPKTSHLWILSGGSCGSSLILFSIHKDQDQMNIPFLSVERKSISSLSVNNDQVSQKFFSSDFSDKKKSGIPNYSELNGIVGTSHYNFIYSAIFHENSDLLAKRRRNRFLIPFQSIQEQEQEKEFIPHSGISVEIPINGIFRRNSIFAFFDDPRYRRKSSGILKYGTLKADSIIQKEDMIEYRGVQKFKTKYEMKVDRFFFIPEEVHILPESSAIMVENYSIIGVDTRITLNIRSQVGGLIRVERKKKRIELKIFSGDIHFPDKTDKISRHSGILIPPGRGKTNSKESKNLKNWIYVQRITPTKKKFFVLVRPVATYEIADSINLATLFPKDLFREKDNIQLRVFNYILYGNGKPTRGISDTSIQLVRTCLVLNWDQDNKNSSLEEVRAFFVEVNTKGLIRDFIRIGLVKSHISYIRKRNNPPDSGLISADSMNPFYSISPKAGILHQSLRQNHGTIRMFLNRNKESQSLLILSSSNCFRIGPFNHVKYHNVINQSIKKKPLITIKNSSGPLGTAIQISNFYSFLPLLTYNQISVIKYLQLDNFKYIFQVIHSYLIDENGRIFNLDPYSNLVLNPFKLNWYFLHQNYNNNYCEETSTIISLGQFFCENVCIAKKEPYLKSGQVLIVQRDSVVIRSAKPYLATPGAKVHGHYREILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISLNLEKRIKGWNRCITRILGIPWGFLIGAELTIVQSRISLVNKIQKVYRSQGVQIHNRHIEIIVRQITSKVLVSEEGMSNVFLPGELIGLLRAERTGRALEEAICYRAVLLGITRASLNTQSFISEASFQETARVLAKAALRGRIDWLKGLKENVVLGGVIPAGTGFNKGLVHCSRQHTNILLEKKTKNLSLLEGDMRDILFYHREFCDSSI

Entity 5 polymer

描述 description	PAP1
分子量 formula_weight	103467.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

MSLFFLNPALPSNSIHPIPRRAAGISSIRCSISAPEKKPRRRRKQQQKRENEDSSSFGSSEAVSALERSLRLTFMDELMERARNRDPSGVSEVIYDMIAAGLSPGPRSFHGLVVAHALNGDEQGAMHSLRKELGAGQRPLPETMIALVRLSGSKGNAQRGLELLAAMEKLNYDIRQAWLILVEELVRTNHLEEANKVFLKGARGGMRATDQLYDLMIEEDCKAGDHSNALDISYEMEAAGRFATTFHFNCLLSVQATCGIPEVAYATFENMEYGEDFMKPDTETYNWVIQAYTRADSYDRVQDVAELLGMMVEDYKRVQPNVKTHALLVECFTKYCVVKEAIRHFRALKNFEGGTKVLHNAGNFEDPLSLYLRALCREGRIVELIDALDAMRRDNQPIPPRAMIMSRKYRTLVSSWIEPLQEEAELGYEIDYLARYVEEGGLTGERKRWVPRRGKTPLDPDAAGFIYSNPIETSFKQRCLEDWKVHHRKLLRTLQSEGLPVLGDASESDYMRVMERLRNIIKGPAQNLLKPKAASKMVVSELKEELEAQGLPIDGTRNVLYQRVQKARRINKSRGRPLWVPPIEEEEEEVDEEVDELICRIKLHEGDTEFWKRRFLGEGLIETTAETKETDESSVATGEIENKTEVVAKEADDDEDDEEEEQEGDEDDDENEEEEEAVVVEPENRAEGEDLIKNKAADAKRHLQMIGVQLLKESDEANRTKKRGKRASRMTLEDDADEDWFPEEPFEAFKEMRERKVFDVSDMYTIADVWGWTWEKDFKNKTPRRWSQEWEVELAIVLMAKVIELGGVPTIGDCAVILRAAIRAPMPSSFLKILQTTHSLGYAFGSPLYDEIITLCLDLGELDAAIAIVADMETTGITVPDQTLDKVISARQSNEIPKSEHEEPPSSSESS

Entity 6 polymer

描述 description	PAP2
分子量 formula_weight	96112.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINKLSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLEVFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEGIQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNVLLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGYFKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNTMHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDERTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGNYDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGGMYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLKQLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA

Entity 7 polymer

描述 description	PAP3
分子量 formula_weight	79815.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H

MQICQATLTTFTFTNPSNPNFCKPKPLFPSFQPPRRVTLPPCRGFSSDEFPVDETFLEKFGPKDKDTEDEARRRNWIERGWAPWEEILTPEADFARKSLNEGEEVPLQSPEAIEAFKMLRPSYRKKKIKEMGITEDEWYAKQFEIRGDKPPPLDTSWAGPLVVRQIPPRDWPPKGWEVDRKELEFIREAHKLMAERVWLEDLDKDLKVGEDATVDKMCLERFKVFLKQYNEWVEANKDRLEEDSYKYDQDFYPGRRIRGKDYKEGMYELPFYYPGMICEGTVTTLHLYQGAFVDIGGVHEGWVPIKGNDWFWIRHFIRVGMHVIVEITAKRDPYRFRFPLELRFVHPNIDHMIFNKFDFPPIFHRDGDTNPDEIRRDCGRPPEPRKDPGSKPEEEGLLSDHPYVDKLWQLHVAEQMILDDYEANPEKYKGKKLSELSDDEGFDERKEIEHGEAYYKKTKLPKVILKTSVKELDLEAALIERKYHNKLMMEAKARGEGYKIEKLRRNIEMDEYDSLHWRRSLEEREALLRDISSRQALGLPLEEPGRYKPGSFFGKDQYDPTSALYQYDYWGEPKNSEISKQERMKDAHNKSIVGKGNVWYDMSYDDAIKQTIERRKAESNVVTQKEEETESKEEEEDDDDEYEFDDFDYSILSDESSIGYSEQQPLVNGTQVFTD

Entity 8 polymer

描述 description	PAP4
分子量 formula_weight	30273.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	I

MASCLVTTSSFYTVSDSSLRLKPSNLVHLSNQQRRRSLGSRGGLKVEAYYGLKTPPYPLDALEPYMSQRTLEVHWGKHHRGYVDNLNKQLGKDDRLYGYTMEELIKATYNNGNPLPEFNNAAQVYNHDFFWESMQPGGGDMPIKGVLEQIEKDFGSFTNFREKFTNAALTQFGSGWVWLVLKREERRLEVVKTSNAINPLVWDDIPIINLDVWEHSYYLDYKNERGKYINTFLNHLVSWNAAMSRMARAEAFVNLGEPTIPIA

Entity 9 polymer

描述 description	PAP5
分子量 formula_weight	60884.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J

MASISTTSWLYRDKLCTESGKLGTCILQRPVKCGFPVKRLYVGITSKDVLMRDCIKCKKDDDDDDASEGSSKKDGQGYEYVSVERAPYYSYMDSTSGKMEPASGARASIPGEDYWPEGTSSRVRAARAPQPAGESSSFPSYGKNPGSRRKKNRKATEGNAAVETYDEVSDSEDSSEEEESDSSNGFVVYNNEVEGEDEEETGFELDKKLGRPHPFIDPTKKKQIETTLTSDESWWNWRKPEKEQWSRWQRRRPDVETVFLKAMAETGQVKLYGKEPTLTETSLYRARRHLFKEERLQAERERLAKEGPMAFYSEWVKAWKRDTSREAVQKHFEETGEDENTQLIEMFSHQTDREYRIMMGTDVRIKRDPLAMRMKEDQIKQIWGGDPVYPTINYIQAPDAVMDFRGPDFHEPTPNMLSYLKENCKVISREMHETLLAKEKTEQVEVPDIDDAMAQAVDIGENDDEEEDTEEAEKDEKVARNWSVLKSTPELRNSKPKPKKEGRMSLDEAVDDSENLTDFLMDFDEETDP

Entity 10 polymer

描述 description	PAP6
分子量 formula_weight	52435.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K

MASLHLFPHLHHFDSIIHRREIAAHSRRQFLSPKASINGGVVTNGASAAETAKPSRKGRNKKKQTEETNPDSDPALVDYDDGIDFPYDDPPLVCCFGAVQKEFVPVVRVHDNPMHPDIYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMGKVGGDDYGEELVLMMNKERVQTRGVKFDEGASTACTRVKIKFEDGKMKAETVKEPPEDSLLASELNLAVLKEARIFHFNSEVLTSPTMESTLFKAIQWSKKFGGLIFFDLNLPLPLWRSRNETRKLIKKSWDEANIIEVSQQELEFLLDEEYYERRRNYTPQYFAEDFEQTKNRRDYYHYTPEEIKPLWHDDLKLLVVTDGTLRLHYYTPKFDGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTCPEMFEDQDVLERQLRFAVAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW

Entity 11 polymer

描述 description	PAP7
分子量 formula_weight	55675.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

MASPVSLHYLINTFISKPQGFCNGTVSSPRPRSSFVRERQNSVKPIKVASLETQPFPLFQSPASEESSSSELESADPDFYKIGYVRRVRAYGVEFKEGPDGFGIYASKDIEPRRRARVIMEIPHELMITIRQKHPWMFFPDIVPIGHPIFDIINSTDPERDWDLRLACLLLFSFDREDHFWRLYGDFLPAADECSSLLLATEEDLAELQDPQLVSTIRQQQKRVLEFWEKNWHSGVPLKIKRLAEDAERFIWAVSIAQTRCISMKTRIGALVQDLNMMIPYADMLNHSFEPNCFLHWRPKDRILEVMSNAGQAIKKGEEMTINYMPGQKNNMLMERYGFSTPVNPWDAIPFSGDSRIHLNSFLSVFNIFGLPEEYYHDSELSGDDSFVDGAVIAAARTLPTWSDIDLPPIPSAERKAVKELQDECRKMLAEYPTTSEQDQKLLDSLSEARTTFATAVKYRMHRKMFIGKIIKALDIYQERLLF

Entity 12 polymer

描述 description	PAP8
分子量 formula_weight	38039.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

MASSAASPSLSLLSLTPKPPPSPSTASATSHRLFPSFRTNGCFAPLTLKPRRGRSIIVKVDDGDADGGGQDEYDMDDEEVEEVDNKKDYDVEYDPLAAAMAAASGGGGDGDIAFVQSKSFISTQGWDSEMVVDYRINEDEFHKISLLDCDFFIRKPPDPDNDVYDFREMYVTPPDTDIYSVPRVLAPMPQKYIRCAMSDYGCYDVTEPPIDAPRDPLYKSEREISKVFLTKHYRNRRLNDPEFVLDFEEIYVIDSKTKSITRARVLVTVPGGRKRDRKDDLLVIRDNGNSFKIIHVGERDDPTTVIEREEWTKTREDMEKHLRKLRDFSVSNWF

Entity 13 polymer

描述 description	PAP9
分子量 formula_weight	34008.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

MMTSTCSFLSSCSLLPSQEPNQRMQWKRHEKRQFSRKVAVSGVIRAGFELKPPPYPLDALEPHMSRETLDYHWGKHHKTYVENLNKQIVGTDLDGLSLEEVVLLSYNRGNMLPAFNNAAQAWNHEFFWESIQPGGGGKPSGDLLRLIERDFGSFSDFVERFKAAAASNFGSGWTWLAYKANRLDVANAVNPLPKEEDKKLVIVKTPNAVNPLVWDYSPLLTIDTWEHAYYLDFENRRIEYINTFMEKLVSWETVSTRLESAMARAAQREQEGSDTEAEENPDDEEPEVYLDDASEVD

Entity 14 polymer

描述 description	PAP10
分子量 formula_weight	20954.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O,P

MALVQSRALPRLNVSLSPILSTLHAPPSSLFLRREIRPVVTSPFSSSTTGNLPFSPLTHPRKILCPPPRGKFVREDYLVRKLSAQELQDLVKGERKVPLIVDFYATWCGPCILMAQELEMLAVEYESNAMIVKVDTDDEYEFARDMQVRGLPTLFFISPDPSKDAIRTEGLIPLQMMRDIIDNDM

Entity 15 polymer

描述 description	PAP11
分子量 formula_weight	85121.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q

MAFTFFSPHPVFLPLGRTTSSFSYKPVYSPFPRNSRNLQLAAGPARRNSYPNPADDDPPEAPEDSMHGVSKFQQIQRQAARARKLEEEDFEKNRNTYLSAIADVEDAPETGRDDVESGGDLFSDIDRAISMKRSEFVKKGLLQPNPPKTTSSKKLDEEEDDVTDVVDELDEEEVVDLDEIDKLTGLTEVSDEEDWVDEEGNPRINKKKETGSDHHFEFDLDDFGGSKARIVEPKFRLSLAELLDESKVVPISVYGDLDVEITGIQHDSRGVSSGDLFVCCENEGDSVLSEADKRGAVAVVASKEIDIEDTLGCRALVIVEDTEAVLAALASSFYRHPSKNMAVIGVTGTNGKTTTTYLIKSLYEAMGVRTGMFSTVSCYVHGDNKMDSPTTTSPDAVLVQSMMAKMLHNGTEALVMEASPQELASGKCDEVDFDIAVFTNLTREDSDFRGTDEEYRDAEAKLFARMVDPERHRKVVNIDDPNAAFFVQQGNPDVPVVTFAMENTKADVHPLKFELSLFETQVLVNTPQGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGVEEVDAVPGRCELIDEEQAFGVIVDHANTPDGLSRLLDSVRELKPRRIITVIGCAGENERGKRPVMTKIATEKSDVTMLTSDNPGNEDPLDILDDMLSGIGWTMQEYLKHGEHDYYPPLSNGHRLFLHDVRRVAVRCAVAMGEEGDMVVVAGKGHESYQLEGDKKEFYDDREECREALQYVDELHQAGIDTSEFPWRLPESH

Entity 16 polymer

描述 description	PAP12
分子量 formula_weight	18835.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R

MFCSSFTSSISRIGDARSGNSRASSFTFQTQVSCGIQRDDNGRRIWRRRTLTKKDDMLRYKLQRVPFVEEQVRKIKEVGKVMTMDIERLLLSEDNRFEFVNSVAAEATEYVEKNRDEYGGTKKAIFHVLSNRVNDLGFDRPEAYAESDPYKPGPGYLKEYYT

Entity 17 polymer

描述 description	FLN2
分子量 formula_weight	68527.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S

MASLSFTQFLPFPRCSVDVPCLQPHGFVKFRGERWKGKHSFLMVAGRRKLSESAPLDEDDGGNGAVGGKKPTKVPKKSGARTAKKKVVAKDEPLEESSQLLVDSDNVSDNESDTKEPVRRTRKKAAASSDVNEGKTEKKVRRKRTVKKDKEVEDGLVTYDEASDVEEALTVEATDADSEGEEIDLSKHESEDISHTYGWPPLVCCFGSAQHAFVPSGRPANRLLDYERQERMKDAVWAPEKYIRAPGGCAGGVAIALASLGGKAAFMGKLGDDDFGQAMLYYLNVCQVQTRSVKIDSKRVTACSTMKISKRGRLKSTCVKPCAEDSLSKSEINVDVLKEAKMFYFTTHSLLDKKMMSTTLQAIKISKQLGNVIFYDLNLPLPLWQSLEETKSLIQEVWDLADVIEVTKQELEFLCGIEPTEEFDTKNNDSSKFVHYEPETVEPLWHENLKILFVTNGTSKIHYYTKEHNGAVLGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNDKGYLERTARYAIECGVVDQWLLAQTRGYPPKDDMEEEEDDDEEEEMESDPNGIRSITEREYRTSKPYDEPDGPYVMKPVEEREYRKLELVGSMGEDDDSS

Entity 18 polymer

描述 description	PTAC18
分子量 formula_weight	16430.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T

MASLVMATPFSGSLTQCKKKTKNLSVQRAFFKVTCSMQTPLEELYNVKVERKVSQRRLEELGVSRWSVWKTGKCKLPWDWQVDQLVYIEEGEVRVVPEGSKRFMQFLAGDLVRYPKWLEADLFFNAPYSERYCFKAYADD

Entity 19 polymer

描述 description	PRIN2
分子量 formula_weight	20915.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	U

MQMASLDAALFSSRLQFHSSSSSSSSIRCALPTIFNLSSPAGSSCVLKSTQFLKRRLRAKSTNFSLSSLPRRGFVCRAAEYKFPDPIPEFAEAETKKFRDHMAKKLAKRDLFEDSVDEIVGVCTEIFETFLRNEYGGPGTLLVIPFIDMADTLNERELPGGPQAARAAIKWAQDHVDKDWKEWTGTD

Entity 20 polymer

描述 description	DNA (81-MER)
分子量 formula_weight	25062.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polydeoxyribonucleotide
链编号 strand_id	X

TTATTTGGTTCCTAAAATGGAGGTCAGTACGTCCTATCGATCTTCGGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCC

Entity 21 polymer

描述 description	DNA (81-MER)
分子量 formula_weight	24821.9 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polydeoxyribonucleotide
链编号 strand_id	Y

GGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCCCGCGCGCCGTAGGACGTACTGACCTCCATTTTAGGAACCAAATAA

Entity 22 polymer

描述 description	RNA (40-MER)
分子量 formula_weight	12819.7 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	Z

CCUGAUGAUUAAAUAAACCAAGGAUUUUACCCGGCGCGCG

Entity 23 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 24 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 25 non-polymer

描述 description	FE (III) ION
分子量 formula_weight	55.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 26 non-polymer

描述 description	S-ADENOSYL-L-HOMOCYSTEINE
分子量 formula_weight	384.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 27 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	252

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.62 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	417374

fit	/app/data/saxs_computation/rd/8rdj/8rdj.fit
pddf	/app/data/pr_computation/rd/8rdj/8rdj.pr.out
profile	/app/data/saxs_computation/rd/8rdj/8rdj.dat

Plastid-encoded RNA polymerase transcription elongation complex (Integrated model)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (27)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 non-polymer

Entity 24 non-polymer

Entity 25 non-polymer

Entity 26 non-polymer

Entity 27 water

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)