Polymerase acidic protein
Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric(3) Consistent with protein copy count | Chain A; UniProt 1–716 | Mutation:E349K; R490I | RNA-directed RNA polymerase catalytic subunit × 1 (S5ME50) Polymerase basic protein 2 × 1 (X5F427) MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY cryo-EM buffer:pH 8 cryo-EM vitrification conditions:Cryogen ETHANE | Resolution 3.21 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8RMS | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 6TU5 Influenza A/H7N9 polymerase core (apo) Deposited 2020-01-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain AAA
201–716(516 aa)
Chain DDD
201–716(516 aa)
|
Not recorded | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;281 K;Protein at about 9 mg/ml
0.1 M Tris pH 7.0, 8-13% PEG 8K, 0.2 M MgCl2, 0.1 M guanidine hydrochloride
|
Resolution 3.33 Å R-free 0.268 |
| 7QTL Influenza A/H7N9 polymerase elongation complex Deposited 2022-01-14 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 3 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Not recorded | 2TM 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine × 1 MG MAGNESIUM ION × 2 GTA P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 7R0E Early transcription elongation state of influenza A/H7N9 polymerase backtracked due to double incoproation of nucleotide analogue T1106 and with singly incoporated T1106 at the +1 position Deposited 2022-02-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 3 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Not recorded | MG MAGNESIUM ION × 2 GTA P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.51 Å |
| 7Z4O Influenza A/H7N9 polymerase core dimer with Pol II pSer5 CTD peptide mimic bound in site 2A Deposited 2022-03-04 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 4 PDB declaration: pentameric |
Chain AAA
201–716(516 aa)
|
Not recorded | MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;277 K;0.1 M Tris pH 7.0, 13 % PEG 8K, 0.2 M MgCl2, 0.1 M guanidine hydrochloride
|
Resolution 3.41 Å R-free 0.273 |
| 7Z4O Influenza A/H7N9 polymerase core dimer with Pol II pSer5 CTD peptide mimic bound in site 2A Deposited 2022-03-04 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–RNA Heteromer;Protein × 4 PDB declaration: pentameric |
Chain DDD
201–716(516 aa)
|
Not recorded | MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;277 K;0.1 M Tris pH 7.0, 13 % PEG 8K, 0.2 M MgCl2, 0.1 M guanidine hydrochloride
|
Resolution 3.41 Å R-free 0.273 |
| 7ZPL Symmetric dimer of influenza A/H7N9 polymerase bound to 5' vRNA hook Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 6 PDB declaration: octameric |
Chain A
1–716(716 aa)
Chain D
1–716(716 aa)
|
Not recorded | MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.12 Å |
| 7ZPM Influenza A/H7N9 polymerase apo-protein dimer complex Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–716(716 aa)
Chain D
1–716(716 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.81 Å |
| 8BEK Early transcription elongation state of influenza A/H7N9 backtracked polymerase with singly incoporated T1106 at the U +1 position Deposited 2022-10-21 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 3 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Not recorded | MG MAGNESIUM ION × 2 GTA P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.86 Å |
| 8PM0 Influenza A/H7N9 polymerase in replicase-like conformation in pre-initiation state with Pol II pS5 CTD peptide mimic bound in site 1A/2A Deposited 2023-06-27 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 5 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8PNP Influenza A/H7N9 polymerase in pre-initiation state with continuous Pol II pS5 CTD peptide mimic bound in site 1A/2A Deposited 2023-06-30 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 4 PDB declaration: pentameric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.49 Å |
| 8PNQ Influenza A/H7N9 polymerase in elongation state with continuous Pol II pS5 CTD peptide mimic bound in site 1A/2A Deposited 2023-06-30 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 4 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | 2KH 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine × 1 MG MAGNESIUM ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.88 Å |
| 8POH Influenza A/H7N9 polymerase symmetric dimer bound to the promoter (PA K289A/C489R) Deposited 2023-07-04 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 6 PDB declaration: decameric |
Chain A
1–716(716 aa)
Chain E
1–716(716 aa)
|
Mutation:K289A; C489R Mutation:K289A; C489R | MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8R3K Influenza A/H7N9 polymerase in self-stalled pre-termination state, with Pol II pS5 CTD peptide mimic bound in site 1A/2A. Deposited 2023-11-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 4 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | POP PYROPHOSPHATE 2- × 1 MG MAGNESIUM ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.43 Å |
| 8R3L Influenza A/H7N9 polymerase in pre-initiation state, intermediate conformation (I) with PB2-C(I), ENDO(T), and Pol II pS5 CTD peptide mimic bound in site 1A/2A Deposited 2023-11-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 4 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.25 Å |
| 8RMP Influenza polymerase A/H7N9-4M (ENDO(R) | Core1) Deposited 2024-01-08 | Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å |
| 8RMQ Influenza polymerase A/H7N9-4M (ENDO(R) | Core2) Deposited 2024-01-08 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | MG MAGNESIUM ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.54 Å |
| 8RMR Influenza polymerase A/H7N9-4M replication complex, an asymmetric polymerase dimer bound to human ANP32A Deposited 2024-01-08 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
1–716(716 aa)
Chain D
1–716(716 aa)
|
Mutation:E349K; R490I Mutation:E349K; R490I | MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.25 Å |
| 8RN0 Influenza polymerase A/H7N9-4M encapsidase plus 627(R) / human ANP32A (from "Influenza polymerase A/H7N9-4M replication complex" | Local refinement) Deposited 2024-01-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain D
1–716(716 aa)
|
Mutation:E349K; R490I | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.13 Å |
| 9FYX Influenza A/H7N9 polymerase pre-cleavage cap-snatching complex Deposited 2024-07-04 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer;Protein × 18 PDB declaration: 23-meric |
Chain a
1–716(716 aa)
|
Mutation:E119D | ZN ZINC ION × 9 G1G 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE × 1 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20 mM BICINE pH 8.0
150 mM NaCl
0.1 mM MgCl2
4% glycerol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9G0A Influenza A/H7N9 polymerase post-cleavage cap-snatching complex Deposited 2024-07-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer;Protein × 18 PDB declaration: 23-meric |
Chain a
1–716(716 aa)
|
Mutation:E119D | ZN ZINC ION × 8 MG MAGNESIUM ION × 2 G1G 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE × 1 PO4 PHOSPHATE ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20 mM BICINE pH 8.0
150 mM NaCl
3 mM MgCl2
4% glycerol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9RAF Influenza A/H7N9 polymerase in complex with a 70-mer template in stalled elongation with backtracking and stem. Deposited 2025-05-20 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 4 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.15 Å |
| 9RAG Influenza A/H7N9 polymerase in complex with a 70-mer RNA template, in stalled elongation. Deposited 2025-05-20 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 4 PDB declaration: hexameric |
Chain A
1–716(716 aa)
|
Mutation:E349K; R490I | MG MAGNESIUM ION × 3 POP PYROPHOSPHATE 2- × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.99 Å |
21 other PDB entries and 22 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | M9TI86_9INFA |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A; PDBConstruct 1–716; UniProt 1–716 |