8sgx

Leishmania tarentolae propionyl-CoA carboxylase (alpha-4-beta-6)

Method: ELECTRON MICROSCOPY D_max: 209.9 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8sgx
沉积日期 deposition_date	2023-04-13
结构标题 title	Leishmania tarentolae propionyl-CoA carboxylase (alpha-4-beta-6)
关键词 keywords	Multienzyme complex, carboxylase, LIGASE; LIGASE
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	63.73 Å
回转半径 Rg (电子) rg_electron	63.35 Å
零角强度 I(0) i0	5354000000.00
分子量 molecular_weight	615020.0 kDa
排除体积 excluded_volume	769030 Å³
包络体积 envelope_volume	1156000 Å³
水化壳体积 shell_volume	150530 Å³
包络直径 envelope_diameter	244.8 Å
壳层 Rg shell_rg	66.33 Å
包络 Rg envelope_rg	63.69 Å
形状 Rg shape_rg	63.30 Å
总 Rg total_rg	63.56 Å
总原子数 total_atoms	43182
残基数 n_residues	5562
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	209.9 Å
Rg (实空间) rg_real	63.68 Å
Rg 误差 (实空间) rg_real_error	1.12 Å
I(0) (实空间) i0_real	5.3470e+09
I(0) 误差 (实空间) i0_real_error	9.2890e+07
Rg (倒空间) rg_reciprocal	63.61 Å
I(0) (倒空间) i0_reciprocal	5352000000.0000
解质量估计 total_estimate	0.8693
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	75.5 Å
偏度 Skewness skewness	0.436
峰度 Kurtosis kurtosis	-0.034
角度范围 angular_range	— – 0.1250 Å⁻¹
当前正则化参数 α current_alpha	0.0231
最高正则化参数 α highest_alpha	505900000.0000
实空间数据点数 n_real_points	26
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.864; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.977; Smooth: 0.726

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (3)

Entity 1 polymer

描述 description	Propionyl-coa carboxylase beta chain, putative
分子量 formula_weight	53587.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C,D,E,F,G,H
原子数 atom_count	43182

PTAAEDLRHKKKRLTAMERVQLFCDPGTFRERDALVEHECHNFGMEKRKVPGDGFITGTGKVFGRPVFLFSHDFTVFGGSLSRTNAAKVVRIMEEAAKIGVPVIGFNDSGGARIHEGVDSLAGYADIFLRNTLFSGVIPQISVIMGPCAGGAVYSPAITDFTFMVETSSYMFVTGPEVVSAVGGKLVTKDELGGPHVHATKSGVSAGTFPNDIVAMAQLRRLYSYLPLSNRDPVPVLPTADERYRDVSSLNTVVPTEVKEAYDMRDVIYPVIDHDSFFEIQPQFAKNIICGFARVEGRSVCIIANQPKVQAGVLDIDSSVKGARMVRFADAFNIPIITFVDVPGFLPGVQQEYGGIIRHGAKLLYAYAEATVPKVTIITRKAYGGAYDVMSSKHLRGDSNYAWPHAEIAVMGAAGACKLLYSKETAEQQAQRIADYEKTFCTPLSAARKGFVDAVIDPSETRMRVCEDLERLARKQLQNPWKKHGNIPL

Entity 2 polymer

描述 description	propionyl-CoA carboxylase
分子量 formula_weight	73277.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S,V,X,Z

VLVANRGEIACRVMATCRRLGIKTVAVYSTADEQAKHVKVADESVCIGPPASVESYLCIDKIVDACKKTGAQAVHPGYGFLSENGEFQSALQKNNIVFVGPDAHSIESMGDKIESKRLAQRAGVTCIPGFIGEVKTHEDLLRFAREIGYPVMIKASGGGGGKGMRVAYNDTQCVEYYDMCREEAKAAFHSDKMLVERFIDHPRHIEIQVIADRRGNTVYLPERECSIQRRNQKVIEEAPSVLLDATTRKAMGEEAVAMARAVQYVSAGTVENVVNPQKQFYFLEMNTRLQVEHPITEEITGVDLVEQMLRAAADLPLSITQDDITINGHATECRVYAEDPMKNYFPSIGRLTMYQEPTGAGVRCDSGIIEGSQISVYYDPLICKLSTWGRDRAECIGRMEKALDEYVIRGLRHNICLLRDVVTEPRYRSGSITTNYLQEQYPNGFKKAELTAEEMQLMYEVAACVHLKRERLHYTQGTAPSERQLYLSVGAGQEGETPVYVRYLDDSHFEIGASKHGPFRKMEVVWKASYPIIRVKDGEAETVLQFWGTNEVTYGMQMRGTTFDVNVMSDLQSTLAHFVPITEATTNTKQILSPMPGVIVAIKVQPGQMVVAGEELLTLEAMKMRNKIHAQADGKVKEVKVKLGATVEDNEVLVELE

Entity 3 non-polymer

描述 description	5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL
分子量 formula_weight	228.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

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7. 引用文献 (1)

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分辨率 resolution	10.30 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	5933

fit	/app/data/saxs_computation/sg/8sgx/8sgx.fit
pddf	/app/data/pr_computation/sg/8sgx/8sgx.pr.out
profile	/app/data/saxs_computation/sg/8sgx/8sgx.dat

Leishmania tarentolae propionyl-CoA carboxylase (alpha-4-beta-6)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (3)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)