8tek

Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila

Method: ELECTRON MICROSCOPY D_max: 237.9 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8tek
沉积日期 deposition_date	2023-07-06
结构标题 title	Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila
关键词 keywords	complex, Cilia, axoneme, nexin-dynein regulatory complex, PROTEIN BINDING; PROTEIN BINDING
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	82.76 Å
回转半径 Rg (电子) rg_electron	84.62 Å
零角强度 I(0) i0	658884000.00
分子量 molecular_weight	216810.0 kDa
排除体积 excluded_volume	273270 Å³
包络体积 envelope_volume	575520 Å³
水化壳体积 shell_volume	69752 Å³
包络直径 envelope_diameter	340.1 Å
壳层 Rg shell_rg	57.18 Å
包络 Rg envelope_rg	83.80 Å
形状 Rg shape_rg	84.56 Å
总 Rg total_rg	84.11 Å
总原子数 total_atoms	15250
残基数 n_residues	1817
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	237.9 Å
Rg (实空间) rg_real	80.76 Å
Rg 误差 (实空间) rg_real_error	1.49 Å
I(0) (实空间) i0_real	6.4960e+08
I(0) 误差 (实空间) i0_real_error	1.0500e+07
Rg (倒空间) rg_reciprocal	78.88 Å
I(0) (倒空间) i0_reciprocal	651800000.0000
解质量估计 total_estimate	0.8361
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	87.7 Å
偏度 Skewness skewness	0.390
峰度 Kurtosis kurtosis	-0.596
角度范围 angular_range	— – 0.0950 Å⁻¹
当前正则化参数 α current_alpha	0.0603
最高正则化参数 α highest_alpha	33320000.0000
实空间数据点数 n_real_points	20
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.949; Stabil: 0.989; Sysdev: 1.000; Positv: 1.000; Valcen: 0.947; Smooth: 0.022

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (10)

Entity 1 polymer

描述 description	Dynein regulatory complex protein 1/2 N-terminal domain-containing protein
分子量 formula_weight	98840.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	15250

MNPSQTQKGKIQIQVSRGSIDLLVRTMYEDINNIKLKNERFQKKKRAEDERRRRERYAEIQQEAIASGKKNAALEMKWAELKELDECEELNREMQDQQQTFQQIIEGKENLRKEFEEELKRKDDEYVKMLKEQSNDIKDMISKMRSQFYKIRDANMQELEDIERHFEQERSNLLNEFKAKIQATFKKHLDLEEQYVKEHAKTEEEQTKNIEDLRIKGAKHYAELKITMETEIQDLEKCFEDMKALYQLITEKLDYSLKVLQEKFHENNSMCDELKRKENNFKNRLKQLTKDYKQYDKKFKLENKNLTQEYKRIIRQFKELQKKFKHFEKADLDKYSEIQKMNEAEVKELKDKIIKCNITIHIQQLGMQWIPPQSEEEILAQQKLQLEQGGKAEEEEEREFEFCISEIKIGEICNIILEEADFLIDDKLREELEGSAEPEQIFQRLDCIKKCLYIDSEDEMNLFFRELITKCRVKSAEEIEEEARKQLLLLGLIKEAEENENPEGEEVQQQEGAQQKKKEEGNKKDDPKGKEGAKDSKENANPQGQNKSNQDERKKKKDGQDDHAGQGQGQYDSNQGGMGENQEEGENAEGQENLEGEIEKEIDLENTQINLNEVVKFLQNWQANKDKRKEKLEKESSKKAVKQETEREKKERIAREGKKYWEKLTQVLPERTFRIWNVNFFKFSFEKQILLNQINKLRFWTDLCPNIMNFFWTDKNQSKKPVNCITKMKNQRIFQINTYKLIMNLLSALQDSLIQIKDKDDFIQTKISTNLFTFHNFQILLQIFIVNQSFLFIRLCQSYSKKIILLIKNINQKLFNLSNFKNQFSF

Entity 2 polymer

描述 description	Coiled-coil protein, putative
分子量 formula_weight	60596.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

MALLGKTLKGRSTRPGGIPQKRNIKWRQLAKNQEEFDQLKQLAKMKREGLKARIKDEQKVVTFNKKKLITYWRKIMRIAKTEQLKNEIDIYSQNNQRELDSKEAFIQMLDKNLDEAEDQFQIALRNHLIHIENLMQLQEARMRGLAEEFNRDVNILETEFDLEREEMVKTHKTQLKELEDMIETVKEEDKKKTEEAQNEFSQFKEETKNKNLEETNVMKIILETKQTKYYTELEQMNSKFQSDTSNKVKDHQFYHAHNKNRKQEIDRYLRTISSKKAKIDLMKLKILQHCKEFNARNSALKKEKENISRNYHELKLKMQKFREEESRRLKELSNNSRNAVLKLREYCALGEKILKTAELCRRLETEKEKVLPFYESSVDEDQIPEQLKNEFEHLKKEDAEEYAYLNNFYKRYNKVLLDKLAIEKQKENLQRDNQLLKSLLKQYLDGISLNDDVLKNENNPLLVVNHKFNLGKMPVEKIENKTVIEGVFEVRNTSHQLQGQRAPPFQ

Entity 3 polymer

描述 description	Growth-arrest-specific microtubule-binding protein
分子量 formula_weight	56190.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MSKAAKAKKNVPVVSKLEADARKAAEGVNDNESEEFKKAMRKEARALVEQFNEEKKLLAFYQQERQKINYNWIIAKKELEDKKSELINKEREIQDLQENHFMTLNVYKQKIKHLLFQNQDQQSELKKDVEVTLKQLEDQHRIKSRELKTDVRSLKVTKKEQEISQQDYLFALKSEHDKQMTLMRQDYERQVNDIKRKYDLKMQNLTKEMEEARAAMIKQLEDNKNQKIAEIIKEHTQKYNDIKNYYSEITATNLDYRKTLKNEIKELQTKDEEYKKTLQQTEKGYKELNEPLQALGIEIIQLKKQDEEQEAIIKEKEELKQKIDNQERLFRKLEYEYEVKLQQFQYLERERNALYAKFNQTVFEIHQKSGLENLILEKKVTNLREDLEIKDLQIHQVLTAANIDPNSVGSINKSLEEVESLKNELISELQAQLKQIRKAHSHMVKAYEGKLSEFVIPVEELGFDPLVPTNTD

Entity 4 polymer

描述 description	Growth-arrest-specific microtubule-binding protein
分子量 formula_weight	55393.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MPPKKAKGKKKKEEEPDDEYKSMTGADLTQTLEKLKERVNEMRTNRNYIQMDRDMVENFYHNTLKEISEVKTKISNKETEAEEKESKHRIDVKVFLQKVKHLEYEQEKSNLNIEDDGKKAKEKEDAYFEDITKNMKQLKTQLKSEYLEKEKANIQQVQEEKKDHQSLLKIQQKKFDELINNLIIKYEERLAKLKEDLELKLKVEIHELEERKNLHINELMNNHEKAFAELKKYYNDITAENLNLIKAHKEKIAQIYANIQLNTKNVADNQAKNEQLKEPLAKHREIRNKLKEDLKQFAKHKMSLQNLKSKAITLKDKITKLERDGKDLDEKYEKVVREKQELEKKFEDITQEVKKNADLNNNVLSNRLQILLKEYNNKEEELRTIIDNAGLDHNLHEQLKQRVQQSIEAKNTLIKNLKYSIHHATKAYNDAIRVYEAKLVEFGIPIEELGFQPLETITSSMPAGLVSS

Entity 5 polymer

描述 description	Cilia- and flagella-associated protein 91
分子量 formula_weight	76154.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

MATTTNILHDVVRDQSMVNYVSNRDRPLYFKRPLVPQMTDIPLHISRPPVDQQVDYQTMQMQQNATIVEAPTKDAFVQTDYRESETQTDPYTPKCFVRDGDHPEVMELKDYKYGKGLPASIEELEQIELNREKVWFENSLPPISDEASFNLRRKLMEEQELREWSKKENEIKKFQNEKLYLLQQALIEREKEVEDKSQERIEEIRQQKTEHKNRQIAKIQRKKIKIDRKMTKSRKMQGKETLKRDIIEDYANFASRVYAGITHEGLSLDKIANKYEVQPLALGNYEMLQALHEGIRPREFETRVNLKKEIKEIEKNYTRLENYHRGELKKAQDEINGVHEQSKAQQKQEGNNFSYRNFENKIRPATPTWKYDTDFNISPSLITEIQEYRGPNGVQLMQAADKREEAVLLLQRLLRGRTTQNIMYEGKKKRTALIEELLTVAQIENLEEDKAEEVLMQQHEEKVKNAVLESIQGEVIAETMDELSKELLRIKQERKIQQMVEIAEKDRRIREIEEAGKRQAEEILRDREDVLHNQLIRVHQGTVDTYLDWLMSNALEQSSARQATIMTNLRKAKFNLPLEDFERKYNNNQTLIKDLVHSFLIPNVQRSKLKKQIQLEEKRFSEAAKRSLQQTITRASNKHANVQN

Entity 6 polymer

描述 description	Flagella associated protein
分子量 formula_weight	114163.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

MDQGEFIQDDNDQDYDQQEGDDENNDYMEDPQQDNNYNQNGDNLDDQGQDDDGGQNNLQQPSVPPEDDEDDDDDFPEYANEQNKRLNEIIKKKRKLIKDISAKIEEKSDRTKVLQEHLKNVEQELLHTQALIDAKNKEIETEDHMKQIAERQSGRIQSELKILEKRSIEQQEKLNDVQNQIFRGNEKMDQYKLEMNWNQEELEQWALAARQKEEDNLTLEKYKRADEVKIKELNLAIEKLTAEVGRKQNELEKEITETQAAQIELDKTAEEFKRQHEERHKLFLQWQEVTEIISKRDLAIREEGENFARIKIEIKSNKDALEERKRILKEAKEENKRIQMANELMERQNIQQISDNKHIEEQLAEKKADVEILKNQVSAFASDLSSKKNRIAILSQELLAKKQRLNAAQKKYQAHQVKLKNEEIMAKQYENDRSYAEEKYRKNEKEKKELEKEIRTQKENLFKHTQELFKHREREANLYGEIQGNMAACRNLQSHITKLNQEFQRQQELLYNAEYQIQLMERRVARAKGERTLEEKKDLENEIMNAERELGGVTGQNKELIESLKNLDDEIRTVKKKLAYVEEENTKYSSLIEELILENDMTYQDLNKIIKQKEEVLVQHDTMKLEIKKINESLNGATEKVFNLENQIYQLEMSMQEREKEIEVHKDVLMTEHKAAEEERHKIAVELAERKNKVKNLKIKYESLVQKNKASNGEVESVHEHSQAYYVIKAAQEREELQRKGDELNAKILKNEKELKALDNTLNHLKNRNSNYRDKFLNKGVTTQHREEAEGLEEQCRAASQNLFKKRDELQKLQKEQEEDTRRYTEIKNKLERLYEQHQSLDQEISKYQKDIDQQGDKLQRAQNSLQRNYQLAVNKNQNFINPKNPHMIQVKLDNQNNLYKTLLQGLYSLQQDIPELSSVVDEILKEQRIQNNKAPSSIDINSKRSSQSGRSQRSQRSNISNQ

Entity 7 polymer

描述 description	Coiled-coil protein, putative
分子量 formula_weight	105196.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

MSNQQGPEDNNLEDDMAYLPADHPLLAKLQIDLTKQLTDEHERVDQKLIEIDANLKKLEKTKEDIGVRLYSVQQQLAENQMNFEQAHENYNWVQKLRIEAEQKLKTESEVYDAKKKELEELRKKYLKAQDELSKLTRTLYQINEFNQQMKGQIINTKTNTYRAEENVVNLEAQKKKQDLLIDTMNEEIKRQTEQKTILTAQLISQKEETEQAKQILKEAHLEMQKIIASKKNLLERWQKSLMTMQRMDNALQAIKEALKGQQELNLQIGTELNGVNAEIRKETEIQESLEGKNKKFDYEKDYLQKKYNELQEEKSKLEAQINLLTQSLRQTETEAGRAEIDKRNIEDQMNLIETNIMKLHTETKKLWEDLVHQKSEHTTIEKTATNLNKQANQISIEIEDKSVELENLLNEIARVKIDQLNTLSQIEVLENKRREVIKEREEKEITVATYEVQIRQGHDLNEKKQHEVGRLNREHDKLSSVQSDMSRGPLEAKRNNLIRKTQELGKENDLMQREWIKKQTLLVTQNNRLNKIEEDVSQLKTKQTILEQKKLRLNNNYRIYEKDIREIQNALKNLRNEMNKLNDAIYRNKEKQQKLDNENFNIKSEFVEKLKELEKESVKLEVEIDRLKEEKADLLAEIVESERQILLWERKIQLEKEMQDALDPTVGQTEIQELKREIHRMELRLDDLRKKQEAIIAEMERAVYKRETIQLKYMNKDKTFSNSNSMSQKSSSISAASDNSAQITKKIAQLKTTLNQTTRNAEQMEKAIKNKKIELDDLNAQIEGNNDNLQKLESDCYNKNIELTKHKLERSTNILSISCMQNKAKKLEDLVAGKARLSVPEATLMTKYEELRDKNQEIKEALQKLCDDAPQYVEVLNYLIDLNVGDDDEDQEQ

Entity 8 polymer

描述 description	DUF4201 domain-containing protein
分子量 formula_weight	94540.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P

MASEDGEMPSQFEGIDPEQLTESQMQQYMMEMQRQGLLDSNMEGGQYEEGYEEGQEGEGYGEEYGDQDYDGNQNEYDSQQDSQQQGHDQVNEMHQDRYDRRVNSEISGNYEADDETLRKIRRDLLDNINLERRHRDLQPVYIDLTTNNISQYYSEYLVNNEHNQDFYENAKVRYNNLGECELCHITAKFEPDADITKEYIYDYFMEIGYLFLESEEEKRIILNPANNHIGIGVFFDEIQIVVVLILSEKVLCIQKISQPEQNKIEIRGKMLDENFGIYAIRIMNVDDQKKDIKGVGPEFIEYTRSTQEWMASFELELYNQDRMAIEYYTRVSPDSIPYKKKQSKNEKLTYKHLQLRLRTPFQIYPDPKYAAEDEKERIRKEQEILAHEEQERKEREENDAKRLKQSRKDYGDGQYDDDEHGQDDFNSQSDISDKDKHHDSMHAEQEQNQQAAQQQQDTISNKEIRQELEMAITEAQRQHDEFMLQNHKLQEEIKLLKNKNDGFVDRSNETAMNEHKYLNTLAHVHQIRLDLKQTQTRYNQMSQELQKKLEQKQKKCNEIKYAFLELKREVAKKAANSRTDKPIPEQQINEWEKAELQKSKELQELRLQILRLRNAYVKNQKILKKKEELAEGLHLIDFEQLKIENQTLNEKIEERNEELHKLKKKNTTTIQILTHTREKLGFVQGENGELNSQNVRKDQELDDMRKQLTQQKKTKDKLRSVNLTLKQQTGIVNSEELGQDYRDLRTRVSKLEEEKKRLEQKLRSMHEAIKTANQISTQNMQSQNNSLKKPYQPY

Entity 9 polymer

描述 description	Flagellar associated protein
分子量 formula_weight	42931.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T

MNQDKHPEITDEEQELITVEELSQKLELLYKDMEQIRRENLLFEAYLARNRKEIAKEDEVSEDKKGKGKKKDKNVDKKSLLLTNEEKFEIAQQEQDALKKQIDDGRIKSDQILETLRAILEETDMAITEIRKDAFDFQREILVGGENSRTGKIEAEKIIKFFEEKERQKDALIAKYSSKRTNLERQILKTNNQIQKKEEMGDDLKFIDFYQLQIENKKYVKEIDDKNKKLLALKISTNRISQTLKDEKQNLKQELDKGKEYASQMSERKKKISKIDAQIKSVKKVTSKLEKDRKIYDKQKEIFVQDNQDDVPQIMKYVQYKSKEQQLLYAIQNLERKIEIAELAYKKANRILQSSQQFQQK

Entity 10 polymer

描述 description	CFAP20
分子量 formula_weight	22976.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	g

MFKNTFQSGFLSILYSIGSKPLQIWDKSIRNGHIKRITDQDILSSVLEIMGTNVSTNYITAPADPKETLGIKLPFLVMIIKNLKKYYTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDEGWNQIQFNLSDFTRRAYATNYIETLRVQIHANCRIRRIYFSDRLYSEEELPPEFKLFLPIQGQNKTNV

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	triclinic

分辨率 resolution	3.60 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	211502

fit	/app/data/saxs_computation/te/8tek/8tek.fit
pddf	/app/data/pr_computation/te/8tek/8tek.pr.out
profile	/app/data/saxs_computation/te/8tek/8tek.dat

Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (10)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)