8uu7

Cryo-EM structure of the Listeria innocua 70S ribosome in complex with HflXr, HPF, and E-site tRNA (structure II-B)

Method: ELECTRON MICROSCOPY D_max: 295.1 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8uu7
沉积日期 deposition_date	2023-10-31
结构标题 title	Cryo-EM structure of the Listeria innocua 70S ribosome in complex with HflXr, HPF, and E-site tRNA (structure II-B)
关键词 keywords	cryo-EM, recycling, HflXr, HPF, time-resolved, RIBOSOME; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	85.64 Å
回转半径 Rg (电子) rg_electron	86.09 Å
零角强度 I(0) i0	151722000000.00
分子量 molecular_weight	2161900.0 kDa
排除体积 excluded_volume	2224100 Å³
包络体积 envelope_volume	4061500 Å³
水化壳体积 shell_volume	362730 Å³
包络直径 envelope_diameter	285.5 Å
壳层 Rg shell_rg	99.87 Å
包络 Rg envelope_rg	84.09 Å
形状 Rg shape_rg	86.14 Å
总 Rg total_rg	86.05 Å
总原子数 total_atoms	145236
残基数 n_residues	10433
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	295.1 Å
Rg (实空间) rg_real	87.43 Å
Rg 误差 (实空间) rg_real_error	1.36 Å
I(0) (实空间) i0_real	1.5000e+11
I(0) 误差 (实空间) i0_real_error	3.0770e+09
Rg (倒空间) rg_reciprocal	87.13 Å
I(0) (倒空间) i0_reciprocal	152400000000.0000
解质量估计 total_estimate	0.8997
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	114.7 Å
偏度 Skewness skewness	0.329
峰度 Kurtosis kurtosis	-0.060
角度范围 angular_range	— – 0.0900 Å⁻¹
当前正则化参数 α current_alpha	1.2550
最高正则化参数 α highest_alpha	7647000000.0000
实空间数据点数 n_real_points	19
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.801; Stabil: 0.920; Sysdev: 1.000; Positv: 1.000; Valcen: 0.910; Smooth: 0.631

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (56)

Entity 1 polymer

描述 description	16S Ribosomal RNA
分子量 formula_weight	501949.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	a
原子数 atom_count	145251

UUUAAAGAGAGUUUGAUCCUGGCUCAGGACGAACGCUGGCGGCGUGCCUAAUACAUGCAAGUCGAACGAACGGAGGAAGAGCUUGCUCUUCCAAAGUUAGUGGCGGACGGGUGAGUAACACGUGGGCAACCUGCCUGUAAGUUGGGGAUAACUCCGGGAAACCGGGGCUAAUACCGAAUGAUAGAGUGUGGCGCAUGCCACGCUCUUGAAAGAUGGUUUCGGCUAUCGCUUACAGAUGGGCCCGCGGUGCAUUAGCUAGUUGGUAGGGUAAUGGCCUACCAAGGCAACGAUGCAUAGCCGACCUGAGAGGGUGAUCGGCCACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUAGGGAAUCUUCCGCAAUGGACGAAAGUCUGACGGAGCAACGCCGCGUGUAUGAAGAAGGUUUUCGGAUCGUAAAGUACUGUUGUUAGAGAAGAACAAGGAUAAGAGUAACUGCUUGUCCCUUGACGGUAUCUAACCAGAAAGCCACGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGUGGCAAGCGUUGUCCGGAUUUAUUGGGCGUAAAGCGCGCGCAGGCGGUCUUUUAAGUCUGAUGUGAAAGCCCCCGGCUUAACCGGGGAGGGUCAUUGGAAACUGGAAGACUGGAGUGCAGAAGAGGAGAGUGGAAUUCCACGUGUAGCGGUGAAAUGCGUAGAUAUGUGGAGGAACACCAGUGGCGAAGGCGACUCUCUGGUCUGUAACUGACGCUGAGGCGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGAGUGCUAAGUGUUAGGGGGUUUCCGCCCCUUAGUGCUGCAGCUAACGCAUUAAGCACUCCGCCUGGGGAGUACGACCGCAAGGUUGAAACUCAAAGGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAAGCAACGCGAAGAACCUUACCAGGUCUUGACAUCCUUUGACCACUCUGGAGACAGAGCUUUCCCUUCGGGGACAAAGUGACAGGUGGUGCAUGGUUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUGAUUUUAGUUGCCAGCAUUUAGUUGGGCACUCUAAAGUGACUGCCGGUGCAAGCCGGAGGAAGGUGGGGAUGACGUCAAAUCAUCAUGCCCCUUAUGACCUGGGCUACACACGUGCUACAAUGGAUGGUACAAAGGGUCGCGAAGCCGCGAGGUGGAGCCAAUCCCAUAAAACCAUUCUCAGUUCGGAUUGUAGGCUGCAACUCGCCUACAUGAAGCCGGAAUCGCUAGUAAUCGUGGAUCAGCAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCACGAGAGUUUGUAACACCCGAAGUCGGUAGGGUAACCUUUAUGGAGCCAGCCGCCGAAGGUGGGACAGAUAAUUGGGGUGAAGUCGUAACAAGGUAGCCGUAUCGGAAGGUGCGGCUGGAUCACCUCCUUU

Entity 2 polymer

描述 description	Small ribosomal subunit protein uS2
分子量 formula_weight	28359.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	b

MPVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQKTVKKVDEAFNFMREVASDNGTILFVGTKKQAQESVRDEAIRSGQYFVNHRWLGGTLTNFETIQKRIQHLKKIEKMEADGTFEVLPKKEVVLLKKEQEKLERFLGGIKDMKGLPDALFIVDPRKERIAVAEARKLHIPIIGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMADAIIEVNQGEELTEAEVAPVEEKAAEETTEA

Entity 3 polymer

描述 description	Small ribosomal subunit protein uS3
分子量 formula_weight	24587.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	c

MGQKVHPIGMRIGVIRDWDSKWYAEKDYADFLHEDLRIRDYVAKRLSDASVSRVEIERAANRVNITIHTAKPGMVIGKGGSEVEALRKNLNELTQKRVHINIVEIKRADLDAKLVAENIARQLEGRVSFRRAQKQAIQRTMRAGAKGIKTQVSGRLGGADIARAEHYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPTKKNNVEGGK

Entity 4 polymer

描述 description	Small ribosomal subunit protein uS4
分子量 formula_weight	22716.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	d

MARYTGPSWKVSRRLGISLSGTGKELERRPYAPGQHGPTQRKKISEYGLQQAEKQKLRHMYGLTERQFKNTFNKAGKLQGKHGENFMILLEQRLDNIVYRLGLARTRRAARQLVNHGHITVDGKRVDIPSYQVSVGQVISVREKSAKNSAIAESLEVSSFVPEYVTFDAETLTGSLNRLPERSELAAEINEAFIVEFYSR

Entity 5 polymer

描述 description	Small ribosomal subunit protein uS5
分子量 formula_weight	17516.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	e

MPEQIDGNKLDLEERVVTINRVAKVVKGGRRFRFTALVVVGDKNGHVGFGTGKAQEVPDAIRKAVEDAKKNMVFVPTVDTTIPHTVVGHFGGGEILLKPASAGSGVTAGGPVRAVLELAGVADVSSKSLGSNTPINMVRATIDGIKQLKNAEDVAKLRGKTVEELLG

Entity 6 polymer

描述 description	Small ribosomal subunit protein bS6
分子量 formula_weight	11512.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	f

MARKYEIMYIIRPNIEEDEKKAVVERFDGILTENGAEIIESKEWGKRRLAYEINDYRDGFYHIVKLNADKADSINEFDRLAKISDDIVRHMVIKEEA

Entity 7 polymer

描述 description	Small ribosomal subunit protein uS7
分子量 formula_weight	17848.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	g

MPRKGPVAKRDVLPDPIYNSKLVTRLINKMMVDGKRGKSQAILYSAFDIIAQETGKDPMEVFEQAMKNIMPLLEVKARRVGGANYQVPIEVRADRRSTLGLRWLVNYARLRGEKTMEVRVAREIMDAANNTGASVKKREDTHKMADANRAFAHYRW

Entity 8 polymer

描述 description	Small ribosomal subunit protein uS8
分子量 formula_weight	14667.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	h

MVMTDPIADFLTRIRNANMVKHDKLELPASKIKKEIAEILKREGFIRDVEYIEDDNAGTIRVFLKYGATGERVITGLKRISKPGLRVYAKSTEVPKVLNGLGIAIVSTSQGVLTDKEARAKQVGGEVLAYVW

Entity 9 polymer

描述 description	Small ribosomal subunit protein uS9
分子量 formula_weight	14473.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	i

MAQVQYYGTGRRKSSVARVRLVPGDGKIVINNRDWEDYIPFAALREVIKQPLVATETLGNYDVLVNVHGGGYTGQAGAIRHGVARALLQVAPEYRPALKSAGLLTRDPRMKERKKYGLKGARRAPQFSKR

Entity 10 polymer

描述 description	Small ribosomal subunit protein uS10
分子量 formula_weight	11703.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	j

MAKQKIRIRLKAYDHRILDQSAEKIVETAKRSGASVSGPIPLPTEKSIYTVLRAVHKYKDSREQFEMRTHKRLIDIVNPTPQTVDSLMRLDLPSGVDIEIKL

Entity 11 polymer

描述 description	Small ribosomal subunit protein uS11
分子量 formula_weight	13812.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	k

MARKTNTRKRRVKKNIESGIAHIRSTFNNTIVMITDTHGNALAWSSAGSLGFKGSRKSTPFAAQMAAESAAKSAQEHGLKTLEVTVKGPGSGREAAIRALQAAGLEVTAIKDVTPVPHNGCRPPKRRRV

Entity 12 polymer

描述 description	Small ribosomal subunit protein uS12
分子量 formula_weight	15216.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	l

MPTINQLVRKPRQSKIKKSTSPALNKGLNSFKRELTDVNSPQKRGVCTRVGTMTPKKPNSALRKYARVRLSNGIEVTAYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGALDTAGVENRGQSRSKYGTKKPKK

Entity 13 polymer

描述 description	Small ribosomal subunit protein uS13
分子量 formula_weight	13739.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	m

MARIAGVDVPREKRIVISLTYIYGIGKQTAKEVLAEAGVSEDTRTRDLTEEELGKIREILDRIKVEGDLRREVNLNIKRLIEIGSYRGMRHRRGLPVRGQNTKNNARTRKGPSKTVAGKKK

Entity 14 polymer

描述 description	Small ribosomal subunit protein uS14
分子量 formula_weight	7164.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	n

MAKKSMIAKQKRTPKYAVQAYTRCERCGRPHSVIRKFKLCRICFRELAYKGQIPGVKKASW

Entity 15 polymer

描述 description	Small ribosomal subunit protein uS15
分子量 formula_weight	10656.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	o

MALTQERKNEIIAEYRVHDTDTGSPEVQIAVLTAEINSLNEHVRVHKKDHHSYRGLMKMVGHRRNLLTYLRKKDVQRYRELIKRLGLRR

Entity 16 polymer

描述 description	Small ribosomal subunit protein bS16
分子量 formula_weight	10404.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	p

MAVKIRLKRMGSKKKPFYRIVVADSRFPRDGRSIETIGTYNPLLDPVEVKIDEEATLKWMHNGAKPSDTVRNLLSREGIMEKFHNQKLGK

Entity 17 polymer

描述 description	Small ribosomal subunit protein uS17
分子量 formula_weight	10057.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	q

MADRNQRKVYTGRVVSDKMDKTITVVVETYKKHGLYGKRVKYSKKFKAHDENNIAKTGDVVRISETRPLSATKHFRLLEVVEEAVII

Entity 18 polymer

描述 description	Small ribosomal subunit protein bS18
分子量 formula_weight	9119.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	r

MAGGRRGGRRRKKVCYFTSNGITHIDYKDVELLKKFVSERGKILPRRVTGTSAKYQRKLTVAIKRSRQMALLPFVAEEK

Entity 19 polymer

描述 description	Small ribosomal subunit protein uS19
分子量 formula_weight	10498.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	s

MGRSLKKGPFVDDHLMKKVEAAAESEKKQVIKTWSRRSTIFPTFVGQTIAVYDGRKHVPVYVQEDMVGHKLGEFAPTRTYRGHAGDDKKTKR

Entity 20 polymer

描述 description	Small ribosomal subunit protein bS20
分子量 formula_weight	9189.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	t

MPNIKSAIKRVKTAETRNSRNASQRSAMRTAIKKFDEAAANNADNAKDLYVEASKKLDSAVSKGLIHKNNAARNKSRLAAKLAK

Entity 21 polymer

描述 description	E-site Phenylalanine tRNA
分子量 formula_weight	24645.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	y

GCCCGGAUAGCUCAGUCGGUAGAGCAGGGGAUUGAAAAUCCCCGUGUCCUUGGUUCGAUUCCGAGUCCGGGCACCA

Entity 22 polymer

描述 description	GTPase HflX
分子量 formula_weight	47610.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	v

MEKKVLIVGISQKQKDFDYSMEELANLAAANNMEVVGEIRQNIDRENRATYVGKGKVDEIKGLAEMQDARLIIFNDELSPSQIRNLEEALELDVMDRTGLILAIFANRAKTKEAQLQVQIAKLQYELPRIFGQGEDMDQQSGKGGLSNRGSGEKKIETDRRTIKHQIRHLQKELDMLVDDREVRRRKRKKNEIPVVSLVGYTNAGKSTTMNGLVRAYSETADKQVFEKDMLFATLETSVREIVLPDNKQFLLTDTVGFVSKLPHQLVKAFRSTLEEARDADLLIHVVDYSDPHYKTMMKTTEETLKVVGVEDVPVIYAYNKADLLEDEMYPKQTGNTIIFSAREEESLEFLTEVIRKELFASYEKATFLIPFEAGQVVAYLNEHADILETEYLENGTQIVAEVSPADLQKLAEYQVAE

Entity 23 polymer

描述 description	Ribosome hibernation promoting factor
分子量 formula_weight	21664.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	w

MLKYNIRGENIEVTEPIRDYVEKKIDKLERYFTETPDANVHVNLKVYSDKNAKVEVTIPLPNLVLRAEETSGDLYASIDLIVDKLERQIRKHKTKVNRKFRDKGAERDYFAYSDVNGSTPPEENEGDFDLEIVRTKQFSLKPMDSEEAVLQMNLLGHSFYVYTDAETNGTNIVYSRKDGKYGLIETN

Entity 24 polymer

描述 description	23S Ribosomal RNA
分子量 formula_weight	951061.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	A

GGUUAAGUUAGAAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAAGAAGGACGGGACUAACACCGAUAUGCUUUGGGGAGCUGUACGUAAGCGUUGAUCCAGAGAUUUCCGAAUGGGGGAACCCACUAUCUUUAGUCGGAUAGUAUCCUUACGUGAAUACAUAGCGUGAGGAAGGCAGACCCAGGGAACUGAAACAUCUAAGUACCUGGAGGAAGAGAAAGAAAAAUCGAUUUCCUGAGUAGCGGCGAGCGAAACGGAAAGAGCCCAAACCAAGAAGCUUGCUUCUUGGGGUUGUAGGACACUCUAUACGGAGUUACAAAAGAAAGUUAUAAAUGAAGCGGUCUGGAAAGGCCCGCCAAAGACGGUAACAGCCCGGUAGUUGAAAUGGCUUUCCCUCCAGAGUGGAUCCUGAGUACGACGGAACACGUGAAAUUCCGUCGGAAUCCGGGAGGACCAUCUCCCAAGGCUAAAUACUCCCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAACAGUUCCUGAAACCGUGUGCCUACAAGUAGUUAGAGCCCGUUAAUGGGUGAUAGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUUUGUUGCAAGGUUAAGCGGAAAAAGCGGAGCCGUAGCGAAAGCGAGUCUGAAUAGGGCGCAUAAGUAACAGGUCGUAGACCCGAAACCAGGUGAUCUACCCAUGUCCAGGAUGAAGGUAAGGUAAUACUUACUGGAGGUCCGAACCCACGCACGUUGAAAAGUGCGGGGAUGAGGUGUGGGUAGCGGAGAAAUUCCAAUCGAACUUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCGAGGUAAAGAGUCAUGGAGGUAGAGCACUGUUUGGACUAGGGGCCCUUCUCGGGUUACCGAAUUCAGAUAAACUCCGAAUGCCAUGUACUUAUACUCGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAAAGGGAAACAGCCCAGACCACCAGUUAAGGUCCCCAAAUAUAUGUUAAGUGGAAAAGGAUGUGGGGUUGCUUAGACAACCAGGAUGUUGGCUUAGAAGCAGCCACCAUUGAAAGAGUGCGUAAUAGCUCACUGGUCGAGUGACCCCGCGCCGAAAAUGUACCGGGGCUAAACAUAUUACCGAAACUGUGGAUGAACCUCUUCGGAGGUUCGUGGUAGGAGAGCGUUCUAAGGGCGGUGAAGUCAGACCGGAAGGACUGGUGGAGCGCUUAGAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGAAGGGUGAGAAUCCCUUCCACCGAAUAUCUAAGGUUUCCUGAGGAAGGCUCGUCCGCUCAGGGUUAGUCGGGACCUAAGCCGAGGCCGAUAGGCGUAGGCGAUGGACAACAGGUAGAGAUUCCUGUACCAGUGCUAAUUGUUUAACCGAUGGGGUGACACAGAAGGAUAGGGAAUCGCACGAAUGGAAAUGUGCGUCCAAGCAGUGAGUGUGAGAAGUAGGCAAAUCCGCUUCUUGCGAAGCAUGAGCUGUGAUGGGGAAGGAAAUUAAGUACGGAAGUUCCUGAUUUCACGCUGUCAAGAAAAGCCUCUAGGAAGAGUAGUACUGCCCGUACCGCAAACCGACACAGGUAGAUGAGGAGAGAAUCCUAAGGUGAGCGAGAGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCGGGAGAAGGGGUGCUCUAUUAGGGUGCAAGCCCGAGAGAGCCGCAGUGAAUAGGCCCAGGCGACUGUUUAGCAAAAACACAGGUCUCUGCAAAACCGUAAGGUGACGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGUGCUUAGCUUCGGCGAAGGUACGAAUUGAAGCCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGGCGCAACGAUCUGGGCACUGUCUCAACGAGAGACUCGGUGAAAUUAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGAGCUUUACUGCAACCUGAUAUGGAAUGUUUGUACCGCUUGUACAGGAUAGGUAGGAGCCGAAGAGACGUGUGCGCUAGCAUACGAGGAGGCAAUGGUGGGAUACUACCCUGGCUGUAUGACCAUUCUAACCCGCCACGCUAAGCGCGUGGGGAGACAGUGUCAGGUGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAGAGUAACGGAGGCGCCCAAAGGUUCCCUCAGAAUGGAUGGAAAUCAUUCGCAGAGUGUAAAGGCACAAGGGAGCUUGACUGCGAGACUGACAAGUCGAGCAGGGACGAAAGUCGGGCUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUCCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCGUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGCACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCUAUCCGUCGCGGGCGCAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACACACCGCUGGUGUACCAGUUGUUCCGCCAGGAGCAUCGCUGGGUAGCUAUGUGUGGCAGGGAUAAACGCUGAAAGCAUCUAAGCGUGAAGCCCCCCUCAAGAUGAGAUUUCCCAUUUCUUCGGAAAGUAAGAUCCCUGAAAGAUGAUCAGGUAGAUAGGUUUGGAGUGGAAGUGUAGCGAUACAUGGAGCGGACAAAUACUAAUCGAUCGAGGACUUAACCAA

Entity 25 polymer

描述 description	5S Ribosomal RNA
分子量 formula_weight	37304.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	B

UCUGGUAGUUAUGGCGAGAAGGUCACACCCGUUCCCAUCCCGAACACGGUAGUUAAGCUUCUCUGCGCCAAUGGUAGUUGGGGGCUUCCCCCUGCGAGAGUAGGUCGCUGCCGGGC

Entity 26 polymer

描述 description	Large ribosomal subunit protein uL2
分子量 formula_weight	30568.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MAIKKYKPTTNGRRHMTSSDFAEITTSTPEKSLLRPLKKKAGRNNQGKLTVRHHGGGHKRQYRVIDFKRNKDGIPGRVATIEYDPNRSANIALINYADGEKRYIIAAKGLEVGQTIYSGAEADIKVGNALELKDIPVGTVIHNIEMKPGKGGQLVRSAGTSAQVLGKEGKYVLIRLNSGEVRMILATCRATIGQVGNEQHELINIGKAGRSRWMGKRPTVRGSVMNPNDHPHGGGEGKAPIGRKSPMSPWGKPTLGYKTRKKNNNSDKFIVRRRKKK

Entity 27 polymer

描述 description	Large ribosomal subunit protein uL3
分子量 formula_weight	22880.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MTKGILGRKVGMTQVFTENGELIPVTVIEAAQNVVLQKKTVETDGYEAVQIGFEDKRAKLSNKPEQGHVAKADTTPKRFIREFRDVNLDEYEIGAEVKVDVFAEGDIIDATGVSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKLLPGRMGGEQITIQNLEIVKVDVEKNVLLVKGNVPGAKKALVQIKTATKAK

Entity 28 polymer

描述 description	Large ribosomal subunit protein uL4
分子量 formula_weight	22640.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MPKLSLLKQDGTNAGEITLNDTVFGIEPNEKVVVDVILSQRASLRQGTHKVKNRSEVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLPKKVRRLAIKSILSSKVNEEKLVVLEGLTFDAPKTKEFAAFLKNISVDTKALIVVAGESENVELSARNLQGITVIPAESISVLEVAKHDKLIITKAAVEKVEEVLA

Entity 29 polymer

描述 description	Large ribosomal subunit protein uL5
分子量 formula_weight	20023.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

MNRLKDQYLKEIVPALMSKFNYDSVMEVPKIDKIVINTGVGDATANAKVLDSAVEELALITGQKPVITKAKNSIAGFRLREGMPIGAKVTLRGERMYDFLDKLVTVSLPRVRDFRGVSKKAFDGRGNYTLGVREQLIFPEIDYDQVSKVRGMDVVIVTTAKSDEESHELLTQLGMPFQK

Entity 30 polymer

描述 description	Large ribosomal subunit protein uL6
分子量 formula_weight	19433.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

MSRIGKKTIVIPAGVTVTLNGSTATVKGPKGELVKEFNPEITINIEGNEINVSRPTDNKNHRALHGTTRAILNNMVVGVSEGYEKKLELIGVGYRAQKQGDKLVLNVGYSHPVEFVAPKGVEIEVPANTQVIVKGYNKEHVGELAANIRAVRPPEPYKGKGIRYEGEHVRRKEGKTGK

Entity 31 polymer

描述 description	Large ribosomal subunit protein uL13
分子量 formula_weight	16228.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

MRTTYMAKPGEVERKWYVIDATGVSLGRLSSEVASILRGKNKPQFTPHIDTGDFVIIINAGKIGLTGKKATDKIYYRHSQYPGGLKSRTAGEMRTNNPEKLLELSIKGMLPKNSLGRQLFKKLHVYGGSEHEHAAQKPEVYELRG

Entity 32 polymer

描述 description	Large ribosomal subunit protein uL14
分子量 formula_weight	13248.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

MIQQESRMKVADNSGAREVLTIKVLGGSGRKTANIGDVVVCTVKQATPGGVVKKGEVVKAVIVRTKSGARRQDGSYIKFDENACVIIRDDKSPRGTRIFGPVARELRENNFMKIVSLAPEVL

Entity 33 polymer

描述 description	Large ribosomal subunit protein uL15
分子量 formula_weight	15827.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

MKLHELKPSEGSRKERNRVGRGTGSGNGKTSGRGHKGQKARSGGGVRLGFEGGQLPLFRRIPKRGFTNINRKEFAIVNLDVLNRFEDGTEVTPELLIETGIIRNEKSGIKILSDGNIEKKLTVKANKFSAAAKEAIEAAGGKTEVI

Entity 34 polymer

描述 description	Large ribosomal subunit protein uL16
分子量 formula_weight	16171.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

MLVPKRVKYRREFRGNMRGRAKGGTEVAFGEYGLQAVEASWITNRQIEAARIAMTRYMKRGGKVWIKIFPHKSYTSKPIGVRMGKGKGAPEGWVSPVKRGKIMFEIAGVPEDVAREALRLAAHKLPVKTKIVKREEIGGEANES

Entity 35 polymer

描述 description	Large ribosomal subunit protein bL17
分子量 formula_weight	15237.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P

MGYRKLGRTSSQRKALLRDLATDLIVHERIETTEARAKEIRKVVEKLITSGKKGDLHARRQAAAFIRHEVVEVVQVDAKGKDGSTVKKNRPVYALQKLFDDVAPRYAERQGGYTRILKKGPRRGDGAPMVIIELV

Entity 36 polymer

描述 description	Large ribosomal subunit protein uL18
分子量 formula_weight	13107.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q

MITKIDKNKVRKKRHARVRSKISGTESRPRLNVFRSNKNIYAQVIDDVNGVTLASASNLDKDFGSAESKVDAASKVGELVAKRASEKGITSVTFDRGGYLYHGRVKALAEAARENGLEF

Entity 37 polymer

描述 description	Large ribosomal subunit protein bL19
分子量 formula_weight	13089.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R

MNKLIDEITKSQLNPDVPSFRPGDTVRVHAKVVEGTRERIQLFEGVVIKRRGAGISETFTVRKISNGVGVERTFPVHTPRIAKLEVIRRGKVRRAKLYYLRNLRGKAARIKEIR

Entity 38 polymer

描述 description	Large ribosomal subunit protein bL20
分子量 formula_weight	13719.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S

MPRVKGGTVTRKRRKKIVKLAKGYYGSKHLLFKVANQAVMKSYQYAYRDRRQKKRDFRRLWIARINAAARMQDLSYSKLMHGLKLAGIDINRKMLADLAVNDIASFNTLADSAKKALAK

Entity 39 polymer

描述 description	Large ribosomal subunit protein bL21
分子量 formula_weight	11220.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T

MYAIIETGGKQIKVEAGQEIYVEKLAGEVGDVVTFDKVLFVGGDSAKVGVPFVDGATVTAKVEKQGRAKKLTVYKYKPKKNYHKKQGHRQPYTKLTIDAINA

Entity 40 polymer

描述 description	Large ribosomal subunit protein uL22
分子量 formula_weight	12895.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	U

MASEVTSAKAVAKTVRIAPRKARIVIDLIRGKQVGEAIAILKYTPRSASPIIEKVLKSAIANAEHNYDLDINNLVVEEAFVDEGPTLKRFRPRAQGRASAINKRTSHITVVVSEVKEG

Entity 41 polymer

描述 description	Large ribosomal subunit protein uL23
分子量 formula_weight	10949.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	V

MDARDIIKRPVVTEESTSILDDKKYTFEVDTRATKTQVKYAIEEIFDVKVAKVNVMNYKGKLKRMGRYAGYTNKRRKAIVTVTADSKEIQFFEV

Entity 42 polymer

描述 description	Large ribosomal subunit protein uL24
分子量 formula_weight	11219.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	W

MHVKKGDKVKVITGKDKGKSGKVLAAFPKKDRVLIEGINMVKKHTKPSNINPQGGILNVEAPIHVSNVMLIDPKTGEPTRVGYEVKGDKKVRVAKKSGEVIDK

Entity 43 polymer

描述 description	Large ribosomal subunit protein bL27
分子量 formula_weight	10571.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Y

MLKFDIQHFAHKKGGGSTSNGRDSESKRLGAKRADGQFVTGGSILYRQRGTKIYPGTNVGRGGDDTLFAKTDGVVRFERMGRDKKKVSVYPEVQEA

Entity 44 polymer

描述 description	Large ribosomal subunit protein bL28
分子量 formula_weight	7010.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Z

MAKECVITGRKSRSGNKRSHAMNSSKRTWKANLQKVRILVNGKPKKVWVSARALKSGKVERV

Entity 45 polymer

描述 description	Large ribosomal subunit protein uL29
分子量 formula_weight	7414.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1

MKANDIRDLSTTEIQDQEKALKEELFNLRFQLATGQLENTARIREVRKAIARMKTIVRERELA

Entity 46 polymer

描述 description	Large ribosomal subunit protein uL30
分子量 formula_weight	6504.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2

MAKLEITLKRSLIGRPQPQRKTVQALGLGKTNSVVVKEDNPAIRGMITKVSHLVDVKEV

Entity 47 polymer

描述 description	Large ribosomal subunit protein bL31B
分子量 formula_weight	9274.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3

MKTGIHPEYRPVVFVDTSTDFKFLSGSTKSSSETIKWEDGNEYPLLRVEISSDSHPFYTGKQKHATADGRVDRFNKKYGLK

Entity 48 polymer

描述 description	Large ribosomal subunit protein bL32
分子量 formula_weight	6534.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	4

MAVPFRRTSKAKKRKRRTHVKLQLPGMNECSNCGEYRLSHHVCPECGQYDGKDVANS

Entity 49 polymer

描述 description	Large ribosomal subunit protein bL33
分子量 formula_weight	6021.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	5

MRVNITLECTECGDRNYITTKNKRENPERIELKKYCPRLRRVTLHRETK

Entity 50 polymer

描述 description	Large ribosomal subunit protein bL34
分子量 formula_weight	5319.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	6

MKRTYQPSKRKRKKVHGFRTRMSTKNGRRVLASRRRKGRKVLSA

Entity 51 polymer

描述 description	Large ribosomal subunit protein bL35
分子量 formula_weight	7746.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	7

MPKMKTHRGSAKRFKRTGSGKLKRRHGFTSHMFANKSQKQKRKLRKSAMVSAGDFKRIRQMVAKMK

Entity 52 polymer

描述 description	Large ribosomal subunit protein bL36
分子量 formula_weight	4336.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	8

MKVRPSVKPMCEKCKVIRRKGKVMVICENPKHKQKQG

Entity 53 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	392

Entity 54 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

Entity 55 non-polymer

描述 description	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
分子量 formula_weight	521.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 56 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	15

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.20 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	27543

fit	/app/data/saxs_computation/uu/8uu7/8uu7.fit
pddf	/app/data/pr_computation/uu/8uu7/8uu7.pr.out
profile	/app/data/saxs_computation/uu/8uu7/8uu7.dat

Cryo-EM structure of the Listeria innocua 70S ribosome in complex with HflXr, HPF, and E-site tRNA (structure II-B)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (56)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 polymer

聚合体 Polymer

Entity 34 polymer

聚合体 Polymer

Entity 35 polymer