8uua

Cryo-EM structure of the Listeria innocua 50S ribosomal subunit in complex with HflXr (structure III)

Method: ELECTRON MICROSCOPY D_max: 265.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8uua
沉积日期 deposition_date	2023-10-31
结构标题 title	Cryo-EM structure of the Listeria innocua 50S ribosomal subunit in complex with HflXr (structure III)
关键词 keywords	cryo-EM, recycling, HflXr, time-resolved, RIBOSOME; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	71.40 Å
回转半径 Rg (电子) rg_electron	71.81 Å
零角强度 I(0) i0	61643300000.00
分子量 molecular_weight	1354600.0 kDa
排除体积 excluded_volume	1380400 Å³
包络体积 envelope_volume	2432900 Å³
水化壳体积 shell_volume	259490 Å³
包络直径 envelope_diameter	236.3 Å
壳层 Rg shell_rg	84.36 Å
包络 Rg envelope_rg	71.59 Å
形状 Rg shape_rg	71.86 Å
总 Rg total_rg	71.80 Å
总原子数 total_atoms	90874
残基数 n_residues	6414
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	265.8 Å
Rg (实空间) rg_real	74.47 Å
Rg 误差 (实空间) rg_real_error	1.39 Å
I(0) (实空间) i0_real	6.1660e+10
I(0) 误差 (实空间) i0_real_error	1.1490e+09
Rg (倒空间) rg_reciprocal	72.04 Å
I(0) (倒空间) i0_reciprocal	61740000000.0000
解质量估计 total_estimate	0.8775
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	84.6 Å
偏度 Skewness skewness	0.556
峰度 Kurtosis kurtosis	0.405
角度范围 angular_range	— – 0.1100 Å⁻¹
当前正则化参数 α current_alpha	0.9683
最高正则化参数 α highest_alpha	9728000000.0000
实空间数据点数 n_real_points	23
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.637; Stabil: 0.892; Sysdev: 1.000; Positv: 1.000; Valcen: 0.938; Smooth: 0.898

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (35)

Entity 1 polymer

描述 description	GTPase HflX
分子量 formula_weight	47610.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	v
原子数 atom_count	91008

MEKKVLIVGISQKQKDFDYSMEELANLAAANNMEVVGEIRQNIDRENRATYVGKGKVDEIKGLAEMQDARLIIFNDELSPSQIRNLEEALELDVMDRTGLILAIFANRAKTKEAQLQVQIAKLQYELPRIFGQGEDMDQQSGKGGLSNRGSGEKKIETDRRTIKHQIRHLQKELDMLVDDREVRRRKRKKNEIPVVSLVGYTNAGKSTTMNGLVRAYSETADKQVFEKDMLFATLETSVREIVLPDNKQFLLTDTVGFVSKLPHQLVKAFRSTLEEARDADLLIHVVDYSDPHYKTMMKTTEETLKVVGVEDVPVIYAYNKADLLEDEMYPKQTGNTIIFSAREEESLEFLTEVIRKELFASYEKATFLIPFEAGQVVAYLNEHADILETEYLENGTQIVAEVSPADLQKLAEYQVAE

Entity 2 polymer

描述 description	23S Ribosomal RNA
分子量 formula_weight	951061.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	A

GGUUAAGUUAGAAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAAGAAGGACGGGACUAACACCGAUAUGCUUUGGGGAGCUGUACGUAAGCGUUGAUCCAGAGAUUUCCGAAUGGGGGAACCCACUAUCUUUAGUCGGAUAGUAUCCUUACGUGAAUACAUAGCGUGAGGAAGGCAGACCCAGGGAACUGAAACAUCUAAGUACCUGGAGGAAGAGAAAGAAAAAUCGAUUUCCUGAGUAGCGGCGAGCGAAACGGAAAGAGCCCAAACCAAGAAGCUUGCUUCUUGGGGUUGUAGGACACUCUAUACGGAGUUACAAAAGAAAGUUAUAAAUGAAGCGGUCUGGAAAGGCCCGCCAAAGACGGUAACAGCCCGGUAGUUGAAAUGGCUUUCCCUCCAGAGUGGAUCCUGAGUACGACGGAACACGUGAAAUUCCGUCGGAAUCCGGGAGGACCAUCUCCCAAGGCUAAAUACUCCCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAACAGUUCCUGAAACCGUGUGCCUACAAGUAGUUAGAGCCCGUUAAUGGGUGAUAGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUUUGUUGCAAGGUUAAGCGGAAAAAGCGGAGCCGUAGCGAAAGCGAGUCUGAAUAGGGCGCAUAAGUAACAGGUCGUAGACCCGAAACCAGGUGAUCUACCCAUGUCCAGGAUGAAGGUAAGGUAAUACUUACUGGAGGUCCGAACCCACGCACGUUGAAAAGUGCGGGGAUGAGGUGUGGGUAGCGGAGAAAUUCCAAUCGAACUUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCGAGGUAAAGAGUCAUGGAGGUAGAGCACUGUUUGGACUAGGGGCCCUUCUCGGGUUACCGAAUUCAGAUAAACUCCGAAUGCCAUGUACUUAUACUCGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAAAGGGAAACAGCCCAGACCACCAGUUAAGGUCCCCAAAUAUAUGUUAAGUGGAAAAGGAUGUGGGGUUGCUUAGACAACCAGGAUGUUGGCUUAGAAGCAGCCACCAUUGAAAGAGUGCGUAAUAGCUCACUGGUCGAGUGACCCCGCGCCGAAAAUGUACCGGGGCUAAACAUAUUACCGAAACUGUGGAUGAACCUCUUCGGAGGUUCGUGGUAGGAGAGCGUUCUAAGGGCGGUGAAGUCAGACCGGAAGGACUGGUGGAGCGCUUAGAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGAAGGGUGAGAAUCCCUUCCACCGAAUAUCUAAGGUUUCCUGAGGAAGGCUCGUCCGCUCAGGGUUAGUCGGGACCUAAGCCGAGGCCGAUAGGCGUAGGCGAUGGACAACAGGUAGAGAUUCCUGUACCAGUGCUAAUUGUUUAACCGAUGGGGUGACACAGAAGGAUAGGGAAUCGCACGAAUGGAAAUGUGCGUCCAAGCAGUGAGUGUGAGAAGUAGGCAAAUCCGCUUCUUGCGAAGCAUGAGCUGUGAUGGGGAAGGAAAUUAAGUACGGAAGUUCCUGAUUUCACGCUGUCAAGAAAAGCCUCUAGGAAGAGUAGUACUGCCCGUACCGCAAACCGACACAGGUAGAUGAGGAGAGAAUCCUAAGGUGAGCGAGAGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCGGGAGAAGGGGUGCUCUAUUAGGGUGCAAGCCCGAGAGAGCCGCAGUGAAUAGGCCCAGGCGACUGUUUAGCAAAAACACAGGUCUCUGCAAAACCGUAAGGUGACGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGUGCUUAGCUUCGGCGAAGGUACGAAUUGAAGCCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGGCGCAACGAUCUGGGCACUGUCUCAACGAGAGACUCGGUGAAAUUAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGAGCUUUACUGCAACCUGAUAUGGAAUGUUUGUACCGCUUGUACAGGAUAGGUAGGAGCCGAAGAGACGUGUGCGCUAGCAUACGAGGAGGCAAUGGUGGGAUACUACCCUGGCUGUAUGACCAUUCUAACCCGCCACGCUAAGCGCGUGGGGAGACAGUGUCAGGUGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAGAGUAACGGAGGCGCCCAAAGGUUCCCUCAGAAUGGAUGGAAAUCAUUCGCAGAGUGUAAAGGCACAAGGGAGCUUGACUGCGAGACUGACAAGUCGAGCAGGGACGAAAGUCGGGCUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUCCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCGUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGCACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCUAUCCGUCGCGGGCGCAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACACACCGCUGGUGUACCAGUUGUUCCGCCAGGAGCAUCGCUGGGUAGCUAUGUGUGGCAGGGAUAAACGCUGAAAGCAUCUAAGCGUGAAGCCCCCCUCAAGAUGAGAUUUCCCAUUUCUUCGGAAAGUAAGAUCCCUGAAAGAUGAUCAGGUAGAUAGGUUUGGAGUGGAAGUGUAGCGAUACAUGGAGCGGACAAAUACUAAUCGAUCGAGGACUUAACCAA

Entity 3 polymer

描述 description	5S Ribosomal RNA
分子量 formula_weight	37304.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	B

UCUGGUAGUUAUGGCGAGAAGGUCACACCCGUUCCCAUCCCGAACACGGUAGUUAAGCUUCUCUGCGCCAAUGGUAGUUGGGGGCUUCCCCCUGCGAGAGUAGGUCGCUGCCGGGC

Entity 4 polymer

描述 description	Large ribosomal subunit protein uL2
分子量 formula_weight	30568.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MAIKKYKPTTNGRRHMTSSDFAEITTSTPEKSLLRPLKKKAGRNNQGKLTVRHHGGGHKRQYRVIDFKRNKDGIPGRVATIEYDPNRSANIALINYADGEKRYIIAAKGLEVGQTIYSGAEADIKVGNALELKDIPVGTVIHNIEMKPGKGGQLVRSAGTSAQVLGKEGKYVLIRLNSGEVRMILATCRATIGQVGNEQHELINIGKAGRSRWMGKRPTVRGSVMNPNDHPHGGGEGKAPIGRKSPMSPWGKPTLGYKTRKKNNNSDKFIVRRRKKK

Entity 5 polymer

描述 description	Large ribosomal subunit protein uL3
分子量 formula_weight	22880.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MTKGILGRKVGMTQVFTENGELIPVTVIEAAQNVVLQKKTVETDGYEAVQIGFEDKRAKLSNKPEQGHVAKADTTPKRFIREFRDVNLDEYEIGAEVKVDVFAEGDIIDATGVSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKLLPGRMGGEQITIQNLEIVKVDVEKNVLLVKGNVPGAKKALVQIKTATKAK

Entity 6 polymer

描述 description	Large ribosomal subunit protein uL4
分子量 formula_weight	22640.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MPKLSLLKQDGTNAGEITLNDTVFGIEPNEKVVVDVILSQRASLRQGTHKVKNRSEVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLPKKVRRLAIKSILSSKVNEEKLVVLEGLTFDAPKTKEFAAFLKNISVDTKALIVVAGESENVELSARNLQGITVIPAESISVLEVAKHDKLIITKAAVEKVEEVLA

Entity 7 polymer

描述 description	Large ribosomal subunit protein uL5
分子量 formula_weight	20023.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

MNRLKDQYLKEIVPALMSKFNYDSVMEVPKIDKIVINTGVGDATANAKVLDSAVEELALITGQKPVITKAKNSIAGFRLREGMPIGAKVTLRGERMYDFLDKLVTVSLPRVRDFRGVSKKAFDGRGNYTLGVREQLIFPEIDYDQVSKVRGMDVVIVTTAKSDEESHELLTQLGMPFQK

Entity 8 polymer

描述 description	Large ribosomal subunit protein uL6
分子量 formula_weight	19433.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

MSRIGKKTIVIPAGVTVTLNGSTATVKGPKGELVKEFNPEITINIEGNEINVSRPTDNKNHRALHGTTRAILNNMVVGVSEGYEKKLELIGVGYRAQKQGDKLVLNVGYSHPVEFVAPKGVEIEVPANTQVIVKGYNKEHVGELAANIRAVRPPEPYKGKGIRYEGEHVRRKEGKTGK

Entity 9 polymer

描述 description	Large ribosomal subunit protein uL13
分子量 formula_weight	16228.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

MRTTYMAKPGEVERKWYVIDATGVSLGRLSSEVASILRGKNKPQFTPHIDTGDFVIIINAGKIGLTGKKATDKIYYRHSQYPGGLKSRTAGEMRTNNPEKLLELSIKGMLPKNSLGRQLFKKLHVYGGSEHEHAAQKPEVYELRG

Entity 10 polymer

描述 description	Large ribosomal subunit protein uL14
分子量 formula_weight	13248.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

MIQQESRMKVADNSGAREVLTIKVLGGSGRKTANIGDVVVCTVKQATPGGVVKKGEVVKAVIVRTKSGARRQDGSYIKFDENACVIIRDDKSPRGTRIFGPVARELRENNFMKIVSLAPEVL

Entity 11 polymer

描述 description	Large ribosomal subunit protein uL15
分子量 formula_weight	15827.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

MKLHELKPSEGSRKERNRVGRGTGSGNGKTSGRGHKGQKARSGGGVRLGFEGGQLPLFRRIPKRGFTNINRKEFAIVNLDVLNRFEDGTEVTPELLIETGIIRNEKSGIKILSDGNIEKKLTVKANKFSAAAKEAIEAAGGKTEVI

Entity 12 polymer

描述 description	Large ribosomal subunit protein uL16
分子量 formula_weight	16171.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

MLVPKRVKYRREFRGNMRGRAKGGTEVAFGEYGLQAVEASWITNRQIEAARIAMTRYMKRGGKVWIKIFPHKSYTSKPIGVRMGKGKGAPEGWVSPVKRGKIMFEIAGVPEDVAREALRLAAHKLPVKTKIVKREEIGGEANES

Entity 13 polymer

描述 description	Large ribosomal subunit protein bL17
分子量 formula_weight	15237.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P

MGYRKLGRTSSQRKALLRDLATDLIVHERIETTEARAKEIRKVVEKLITSGKKGDLHARRQAAAFIRHEVVEVVQVDAKGKDGSTVKKNRPVYALQKLFDDVAPRYAERQGGYTRILKKGPRRGDGAPMVIIELV

Entity 14 polymer

描述 description	Large ribosomal subunit protein uL18
分子量 formula_weight	13107.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q

MITKIDKNKVRKKRHARVRSKISGTESRPRLNVFRSNKNIYAQVIDDVNGVTLASASNLDKDFGSAESKVDAASKVGELVAKRASEKGITSVTFDRGGYLYHGRVKALAEAARENGLEF

Entity 15 polymer

描述 description	Large ribosomal subunit protein bL19
分子量 formula_weight	13089.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R

MNKLIDEITKSQLNPDVPSFRPGDTVRVHAKVVEGTRERIQLFEGVVIKRRGAGISETFTVRKISNGVGVERTFPVHTPRIAKLEVIRRGKVRRAKLYYLRNLRGKAARIKEIR

Entity 16 polymer

描述 description	Large ribosomal subunit protein bL20
分子量 formula_weight	13719.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S

MPRVKGGTVTRKRRKKIVKLAKGYYGSKHLLFKVANQAVMKSYQYAYRDRRQKKRDFRRLWIARINAAARMQDLSYSKLMHGLKLAGIDINRKMLADLAVNDIASFNTLADSAKKALAK

Entity 17 polymer

描述 description	Large ribosomal subunit protein bL21
分子量 formula_weight	11220.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T

MYAIIETGGKQIKVEAGQEIYVEKLAGEVGDVVTFDKVLFVGGDSAKVGVPFVDGATVTAKVEKQGRAKKLTVYKYKPKKNYHKKQGHRQPYTKLTIDAINA

Entity 18 polymer

描述 description	Large ribosomal subunit protein uL22
分子量 formula_weight	12895.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	U

MASEVTSAKAVAKTVRIAPRKARIVIDLIRGKQVGEAIAILKYTPRSASPIIEKVLKSAIANAEHNYDLDINNLVVEEAFVDEGPTLKRFRPRAQGRASAINKRTSHITVVVSEVKEG

Entity 19 polymer

描述 description	Large ribosomal subunit protein uL23
分子量 formula_weight	10949.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	V

MDARDIIKRPVVTEESTSILDDKKYTFEVDTRATKTQVKYAIEEIFDVKVAKVNVMNYKGKLKRMGRYAGYTNKRRKAIVTVTADSKEIQFFEV

Entity 20 polymer

描述 description	Large ribosomal subunit protein uL24
分子量 formula_weight	11219.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	W

MHVKKGDKVKVITGKDKGKSGKVLAAFPKKDRVLIEGINMVKKHTKPSNINPQGGILNVEAPIHVSNVMLIDPKTGEPTRVGYEVKGDKKVRVAKKSGEVIDK

Entity 21 polymer

描述 description	Large ribosomal subunit protein bL27
分子量 formula_weight	10571.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Y

MLKFDIQHFAHKKGGGSTSNGRDSESKRLGAKRADGQFVTGGSILYRQRGTKIYPGTNVGRGGDDTLFAKTDGVVRFERMGRDKKKVSVYPEVQEA

Entity 22 polymer

描述 description	Large ribosomal subunit protein bL28
分子量 formula_weight	7010.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Z

MAKECVITGRKSRSGNKRSHAMNSSKRTWKANLQKVRILVNGKPKKVWVSARALKSGKVERV

Entity 23 polymer

描述 description	Large ribosomal subunit protein uL29
分子量 formula_weight	7414.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1

MKANDIRDLSTTEIQDQEKALKEELFNLRFQLATGQLENTARIREVRKAIARMKTIVRERELA

Entity 24 polymer

描述 description	Large ribosomal subunit protein uL30
分子量 formula_weight	6504.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2

MAKLEITLKRSLIGRPQPQRKTVQALGLGKTNSVVVKEDNPAIRGMITKVSHLVDVKEV

Entity 25 polymer

描述 description	Large ribosomal subunit protein bL31B
分子量 formula_weight	9274.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3

MKTGIHPEYRPVVFVDTSTDFKFLSGSTKSSSETIKWEDGNEYPLLRVEISSDSHPFYTGKQKHATADGRVDRFNKKYGLK

Entity 26 polymer

描述 description	Large ribosomal subunit protein bL32
分子量 formula_weight	6534.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	4

MAVPFRRTSKAKKRKRRTHVKLQLPGMNECSNCGEYRLSHHVCPECGQYDGKDVANS

Entity 27 polymer

描述 description	Large ribosomal subunit protein bL33
分子量 formula_weight	6021.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	5

MRVNITLECTECGDRNYITTKNKRENPERIELKKYCPRLRRVTLHRETK

Entity 28 polymer

描述 description	Large ribosomal subunit protein bL34
分子量 formula_weight	5319.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	6

MKRTYQPSKRKRKKVHGFRTRMSTKNGRRVLASRRRKGRKVLSA

Entity 29 polymer

描述 description	Large ribosomal subunit protein bL35
分子量 formula_weight	7746.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	7

MPKMKTHRGSAKRFKRTGSGKLKRRHGFTSHMFANKSQKQKRKLRKSAMVSAGDFKRIRQMVAKMK

Entity 30 polymer

描述 description	Large ribosomal subunit protein bL36
分子量 formula_weight	4336.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	8

MKVRPSVKPMCEKCKVIRRKGKVMVICENPKHKQKQG

Entity 31 non-polymer

描述 description	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
分子量 formula_weight	521.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 32 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	200

Entity 33 non-polymer

描述 description	POTASSIUM ION
分子量 formula_weight	39.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 34 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

Entity 35 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	134

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.70 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	332513

fit	/app/data/saxs_computation/uu/8uua/8uua.fit
pddf	/app/data/pr_computation/uu/8uua/8uua.pr.out
profile	/app/data/saxs_computation/uu/8uua/8uua.dat

Cryo-EM structure of the Listeria innocua 50S ribosomal subunit in complex with HflXr (structure III)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (35)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 non-polymer

Entity 32 non-polymer

Entity 33 non-polymer

Entity 34 non-polymer

Entity 35 water

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)