8v7x
Cryo-EM structure of TTMV-LY1 anellovirus virus-like particle expressed in HEK293
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 8v7x |
| 沉积日期 deposition_date | 2023-12-04 |
| 最后修订 last_revision | 2024-09-04 |
| 结构标题 title | Cryo-EM structure of TTMV-LY1 anellovirus virus-like particle expressed in HEK293 |
| 关键词 keywords | Anello Virus: Virus Like Particle (VLP), VIRUS LIKE PARTICLE; VIRUS LIKE PARTICLE |
| 实验方法 method | ELECTRON MICROSCOPY |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | — Å |
| 回转半径 Rg (电子) rg_electron | 116.40 Å |
| 零角强度 I(0) i0 | 171398000000.00 |
| 分子量 molecular_weight | 3589000.0 kDa |
| 排除体积 excluded_volume | 4500500 ų |
| 包络体积 envelope_volume | 8333900 ų |
| 水化壳体积 shell_volume | 587810 ų |
| 包络直径 envelope_diameter | 333.9 Å |
| 壳层 Rg shell_rg | 126.00 Å |
| 包络 Rg envelope_rg | 106.40 Å |
| 形状 Rg shape_rg | 116.50 Å |
| 总 Rg total_rg | 116.10 Å |
| 总原子数 total_atoms | 253440 |
| 残基数 n_residues | 30900 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 330.6 Å |
| Rg (实空间) rg_real | 116.60 Å |
| Rg 误差 (实空间) rg_real_error | 0.66 Å |
| I(0) (实空间) i0_real | 1.6540e+11 |
| I(0) 误差 (实空间) i0_real_error | 3.1800e+09 |
| Rg (倒空间) rg_reciprocal | 128.20 Å |
| I(0) (倒空间) i0_reciprocal | 178400000000.0000 |
| 解质量估计 total_estimate | 0.8844 |
| 解质量评级 solution_quality | GOOD a GOOD solution |
| P(r) 峰数 n_peaks | 1 |
| 主峰位置 r_peak_primary | 178.0 Å |
| 偏度 Skewness skewness | -0.155 |
| 峰度 Kurtosis kurtosis | -0.555 |
| 角度范围 angular_range | — – 0.0650 Å−1 |
| 当前正则化参数 α current_alpha | 2.0090 |
| 最高正则化参数 α highest_alpha | 90130000000.0000 |
| 实空间数据点数 n_real_points | 14 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.922; Stabil: 0.923; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.000 |