8ved

Cryo-EM structure of antibody T5-1E11 in complex with stabilized H1N1 Influenza Hemagglutinin Trimer (A/Kiev/1/57)

Method: ELECTRON MICROSCOPY D_max: 145.7 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8ved
沉积日期 deposition_date	2023-12-18
结构标题 title	Cryo-EM structure of antibody T5-1E11 in complex with stabilized H1N1 Influenza Hemagglutinin Trimer (A/Kiev/1/57)
关键词 keywords	;Broadly neutralizing antibody, group 1, influenza, cryo-EM, Fab, hemagglutinin, trimer, spike, IMMUNE SYSTEM, viral protein, IMMUNE SYSTEM-VIRAL PROTEIN complex ;; IMMUNE SYSTEM/VIRAL PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	46.48 Å
回转半径 Rg (电子) rg_electron	45.99 Å
零角强度 I(0) i0	987433000.00
分子量 molecular_weight	253710.0 kDa
排除体积 excluded_volume	314570 Å³
包络体积 envelope_volume	458500 Å³
水化壳体积 shell_volume	81265 Å³
包络直径 envelope_diameter	153.7 Å
壳层 Rg shell_rg	51.92 Å
包络 Rg envelope_rg	45.45 Å
形状 Rg shape_rg	45.97 Å
总 Rg total_rg	46.27 Å
总原子数 total_atoms	17840
残基数 n_residues	2130
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	145.7 Å
Rg (实空间) rg_real	46.32 Å
Rg 误差 (实空间) rg_real_error	0.94 Å
I(0) (实空间) i0_real	9.8740e+08
I(0) 误差 (实空间) i0_real_error	1.6690e+07
Rg (倒空间) rg_reciprocal	46.48 Å
I(0) (倒空间) i0_reciprocal	987600000.0000
解质量估计 total_estimate	0.8827
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	55.7 Å
偏度 Skewness skewness	0.210
峰度 Kurtosis kurtosis	-0.491
角度范围 angular_range	— – 0.1700 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	110600000.0000
实空间数据点数 n_real_points	35
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.949; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.984; Smooth: 0.640

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (6)

Entity 1 polymer

描述 description	Hemagglutinin
分子量 formula_weight	64432.0 kDa
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,C,E
原子数 atom_count	17840

MKAKLLILLCALSATDADTICIGYHANNSTDTVDTVCEKNVTVTHSVNLLEDSHNGKLCRLKGKAPLQLGNCNIAGWVLGNPECESLLSNRSWSYIAETPNSENGTCFPGDFADYEELREQLSSVSSFERFEIFPKERSWPNHTTRGVTAACPHAMKSSFYKNLVWLTEANGSYPNLSRSYVNNQEKEVLVLWGVHHPSNIEDQRTLYRKDNAYVSVVSSNYNRRFTPEIAKRPKVRGQSGRINYYWTLLEPGDTIIFEATGNLIAPWYAFALSRGPGSGIITSNAPLDECDTKCQTPQGAINSSLPFQNIHPVTIGECPKYVRSTKLRMVTGLRNIPSFQSRGLFGAIAGFIEGGWTGMMDGWYGYHHQNEQGSGYAADQKSTQNAINCITNKVNSVIEKMNTQFTAVGKEFNKLEKRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKNQLRNNAKELGNGCFEFYHKCDNECMESVKNGTYDYPKYSEESKLNREKIDGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGSGLNDIFEAQKIEWHEGHHHHHH

来源 Source

来源生物 organism	Influenza A virus
NCBI 分类编号 ncbi_taxonomy_id	11320
宿主生物 host_organism	Homo sapiens

Entity 2 polymer

描述 description	T5-1E11 Fab heavy chain
分子量 formula_weight	24777.7 kDa
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H,G,J

QVQLQESGPGLVKPSETLSLTCTVSGASVSSYFWSWIRQPAGKALEWIGRIYTSGNTKSNPSLESRVTMSLDASKDQFSLKLTSVTAADTAVYYCAAGRLDYSDTTGYYKPPPLDYWGQGALVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH

来源 Source

来源生物 organism	Homo sapiens
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 3 polymer

描述 description	T5-1E11 Fab light chain
分子量 formula_weight	24091.9 kDa
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L,I,K

DVVMTQSPLSLPVTLGQPASISCRSSQGLAFLDGNTYLSWFQQRPGQSPRRLIYKVSNRDSGVPDRFSGSGSRTDFTLKISRVEAEDVGVYYCMQGTHWPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

来源 Source

来源生物 organism	Homo sapiens
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 4 branched

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	424.4 kDa
来源方法 src_method	man
拷贝数 entity_count	6

Entity 5 branched

描述 description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
分子量 formula_weight	1235.1 kDa
来源方法 src_method	man
拷贝数 entity_count	3

Entity 6 non-polymer

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	221.2 kDa
来源方法 src_method	man
拷贝数 entity_count	16

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.98 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	463321

fit	/app/data/saxs_computation/ve/8ved/8ved.fit
pddf	/app/data/pr_computation/ve/8ved/8ved.pr.out
profile	/app/data/saxs_computation/ve/8ved/8ved.dat

Cryo-EM structure of antibody T5-1E11 in complex with stabilized H1N1 Influenza Hemagglutinin Trimer (A/Kiev/1/57)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (6)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 branched

Entity 5 branched

Entity 6 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)