8vk7

Structure of Mycobacterium smegmatis 50S ribosomal subunit bound to HflX:50S-HflX-B

Method: ELECTRON MICROSCOPY D_max: 265.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8vk7
沉积日期 deposition_date	2024-01-08
结构标题 title	Structure of Mycobacterium smegmatis 50S ribosomal subunit bound to HflX:50S-HflX-B
关键词 keywords	ribosome splitting, Mycobacterium smegmatis 50S, HflX, disordered 23S rRNA helices, TRANSLATION, RIBOSOME; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	72.85 Å
回转半径 Rg (电子) rg_electron	73.77 Å
零角强度 I(0) i0	67698800000.00
分子量 molecular_weight	1438300.0 kDa
排除体积 excluded_volume	1478600 Å³
包络体积 envelope_volume	2517500 Å³
水化壳体积 shell_volume	263500 Å³
包络直径 envelope_diameter	266.7 Å
壳层 Rg shell_rg	85.14 Å
包络 Rg envelope_rg	73.34 Å
形状 Rg shape_rg	73.81 Å
总 Rg total_rg	73.75 Å
总原子数 total_atoms	96832
残基数 n_residues	7114
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	265.8 Å
Rg (实空间) rg_real	75.57 Å
Rg 误差 (实空间) rg_real_error	1.38 Å
I(0) (实空间) i0_real	6.7700e+10
I(0) 误差 (实空间) i0_real_error	1.3370e+09
Rg (倒空间) rg_reciprocal	73.40 Å
I(0) (倒空间) i0_reciprocal	67790000000.0000
解质量估计 total_estimate	0.8850
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	88.6 Å
偏度 Skewness skewness	0.521
峰度 Kurtosis kurtosis	0.251
角度范围 angular_range	— – 0.1050 Å⁻¹
当前正则化参数 α current_alpha	0.9126
最高正则化参数 α highest_alpha	7232000000.0000
实空间数据点数 n_real_points	22
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.695; Stabil: 0.906; Sysdev: 1.000; Positv: 1.000; Valcen: 0.949; Smooth: 0.841

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (36)

Entity 1 polymer

描述 description	50S ribosomal protein L30
分子量 formula_weight	7022.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2
原子数 atom_count	96832

MAELKITQVRSTIGARWKQRESLRTLGLKKIRQSVVREDNAQTRGLINTVHHLVEVEEVGK

Entity 2 polymer

描述 description	50S Ribosomal Protein L37
分子量 formula_weight	2841.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3

MAKRGRKKRDRKHSKANHGKRPNA

Entity 3 polymer

描述 description	GTPase HflX
分子量 formula_weight	50637.1 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	4

MTYPENSVAPSTGELALEDRASLRRVAGLSTELADVSEVEYRQLRLERVVLVGVWTEGSAADAEASLAELAALAETAGSEVLEGLIQRRDKPDPSTYIGSGKAAELREVVLATGADTVICDGELSPAQLNALEKAVKVKVIDRTALILDIFAQHATSREGKAQVSLAQMEYMLPRLRGWGESMSRQAGGRAGGAGGGVGTRGPGETKIETDRRRIRERMAKLRREIRDMKKIRDTQRGSRRRSEIPSVAIVGYTNAGKSSLLNALTGAGVLVENALFATLEPTTRRGEFEDGRPFVLTDTVGFVRHLPTQLVEAFRSTLEEVVDADLLIHVVDGSDVNPLAQINAVRTVINEVVAEYDIAPPPELLVVNKIDAATGVGLAQLRRALPDAVFVSARTGDGLDKLRSRMGELVESTDATVDVTIPYDRGDLVARVHTDGHVDATEHTDAGTRIKARVPAPLAATLREYATFA

来源 Source

来源生物 organism	Mycolicibacterium smegmatis MC2 155
NCBI 分类编号 ncbi_taxonomy_id	246196
宿主生物 host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 4 polymer

描述 description	23S ribosomal RNA
分子量 formula_weight	1012140.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	A

UAAGUGUUUAAGGGCGCAUGGUGGAUGCCUUGGCACUGGGAGCCGAUGAAGGACGUAGGAGGCUGCGAUAAGCCUCGGGGAGCUGUCAACCGAGCGUUGAUCCGAGGAUGUCCGAAUGGGGAAACCCGGCACGAGUGAUGUCGUGUCACCAGGCGCUGAAUAUAUAGGCGUCUGGGGGGAACGCGGGGAAGUGAAACAUCUCAGUACCCGUAGGAAGAGAAAACAAAAUGUGAUUCCGUGAGUAGUGGCGAGCGAAAGCGGAGGAUGGCUAAACCGUAUGCAUGUGAUACCGGGUAGGGGUUGUGUGUGCGGGGUUGUGGGACCUAUCUUUCCGGCUCUACCUGGCUGGAGGGCAGUGAGAAAAUGUUGUGGUUAGCGGAAAUGGCUUGGGAUGGCCUGCCGUAGACGGUGAGAGCCCGGUACGUGAAAACCCGACGUCUGUCUUGAUGGUGUUCCCGAGUAGCAGCGGGCCCGUGGAAUCUGCUGUGAAUCUGCCGGGACCACCCGGUAAGCCUGAAUACUUCCCAGUGACCGAUAGCGGAUUAGUACCGUGAGGGAAUGGUGAAAAGUACCCCGGGAGGGGAGUGAAAGAGUACCUGAAACCGUGCGCUUACAAUCCGUCAGAGCCCUCGACGUGUCGUGGGGUGAUGGCGUGCCUUUUGAAGAAUGAGCCUGCGAGUCAGGGACAUGUCGCGAGGUUAACCCGGGUGGGGUAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGUAUCCACACAAGAGUGUGUGGUGUAGUGGUGUGUUCUGGACCCGAAGCGGAGUGAUCUACCCAUGGCCAGGGUGAAGCGCGGGUAAGACCGCGUGGAGGCCCGAACCCACUUAGGUUGAAGACUGAGGGGAUGAGCUGUGGGUAGGGGUGAAAGGCCAAUCAAACUCCGUGAUAGCUGGUUCUCCCCGAAAUGCAUUUAGGUGCAGCGUCGCAUGUUUCUUGCCGGAGGUAGAGCUACUGGAUGGCCGAUGGGCCCCACAGGGUUACUGACGUCAGCCAAACUCCGAAUGCCGGUAAGUCCAAGAGUGCGGCAGUGAGACGGCGGGGGAUAAGCUCCGUGCGUCGAGAGGGAAACAGCCCAGAUCGCCGGCUAAGGCCCCUAAGCGUGUGCUAAGUGGAAAAGGAUGUGCAGUCGCGAAGACAACCAGGAGGUUGGCUUAGAAGCAGCCACCCUUGAAAGAGUGCGUAAUAGCUCACUGGUCAAGUGAUUGUGCGCCGAUAAUGUAGCGGGGCUCAAGCACACCGCCGAAGCCGCGGCAGCCAACGUGUUGGCUGGGUAGGGGAGCGUCCUGCAUCCGGUGAAGCCGCCGAGUGAUCGAGUGGUGGAGGGUGUGGGAGUGAGAAUGCAGGCAUGAGUAGCGAUUAGGCAAGUGAGAACCUUGCCCGCCGAAAGACCAAGGGUUCCUGGGCCAGGCCAGUCCGCCCAGGGUGAGUCGGGACCUAAGGCGAGGCCGACAGGCGUAGUCGAUGGACAACGGGUUGAUAUUCCCGUACCCGUGUAUGUGCGUCCAUGAUGAAUCAGCGGUACUAACCAUCCAAAACCACCGUGACCGCACCUUUCGGGGUGUGGCGUUGGUGGGGCUGCAUGGGACCUUCGUUGGUAGUAGUCAAGCGAUGGGGUGACGCAGGAAGGUAGCCGUACCGGUCAGUGGUAAUACCGGGGUAAGCCUGUAGGGAGUCAGAUAGGUAAAUCCGUCUGGCAUAUAUCCUGAGAGGUGAUGCAUAGCCGAGUGAGGCGAAUUCGGUGAUCCUAUGCUGCCGAGAAAAGCCUCUAGCGAGGACAUACACGGCCCGUACCCCAAACCAACACAGGUGGUCAGGUAGAGAAUACUAAGGCGUACGAGUGAACUAUGGUUAAGGAACUCGGCAAAAUGCCCCCGUAACUUCGGGAGAAGGGGGACCCACAUGGCGUGUAAGCCUUUACGGCCCAAGCGUGAGUGGGUGGCACAAACCAGUGAGAAGCGACUGUUUACUAAAAACACAGGUCCGUGCGAAGUCGCAAGACGAUGUAUACGGACUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGACCCGUUAACUCCCUUUGGGGGUGAAGCGGAGAAUUUAAGCCCCAGUAAACGGCGGUGGUAACUAUAACCAUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAACGACUUCUCAACUGUCUCAACCAUAGACUCGGCGAAAUUGCACUACGAGUAAAGAUGCUCGUUACGCGCGGCAGGACGAAAAGACCCCGGGACCUUCACUACAACUUGGUAUUGGUGCUCGAUACGGUUUGUGUAGGAUAGGUGGGAGACUGUGAAGCUCACACGCCAGUGUGGGUGGAGUCGUUGUUGAAAUACCACUCUGAUCGUAUUGGGCCUCUAACCUCGGACCGUAUAUCCGGUUCAGGGACAGUGCCUGGUGGGUAGUUUAACUGGGGCGGUUGCCUCCUAAAAUGUAACGGAGGCGCCCAAAGGUUCCCUCAACCUGGACGGCAAUCAGGUGUUGAGUGUAAGUGCACAAGGGAGCUUGACUGCGAGACGGACAUGUCGAGCAGGGACGAAAGUCGGGACUAGUGAUCCGGCACCUCUGAGUGGAAGGGGUGUCGCUCAACGGAUAAAAGGUACCCCGGGGAUAACAGGCUGAUCUUCCCCAAGAGUCCAUAUCGACGGGAUGGUUUGGCACCUCGAUGUCGGCUCGUCGCAUCCUGGGGCUGGAGCAGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGCACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCUCUAUCCGCCGCGCGCGUCAGAAGCUUGAGGAAACCUGUCCCUAGUACGAGAGGACCGGGACGGACGAACCUCUGGUAUACCAGUUGUCCCACCAGGGGCACGGCUGGAUAGCCACGUUCGGACAGGAUAACCGCUGAAAGCAUCUAAGCGGGAAACCUCUUCCAAGACCAGGCUUCUCACCCUCUAGGAGGGAUAAGGCCCCCCGCAGACCACGGGAUUGAUAGACCAGACCUGGAAGCCUAGUAAUAGGUGCAGGGAACUGGCACUAACCGGCCGAAAACUUAC

Entity 5 polymer

描述 description	5S ribosomal RNA
分子量 formula_weight	38061.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	B

GUUACGGCGGUCCAUAGCGGCAGGGAAACGCCCGGUCCCAUCCCGAACCCGGAAGCUAAGCCUGCCAGCGCCGAUGAUACUACCCAUCCGGGUGGAAAAGUAGGACACCGCCGAACAC

Entity 6 polymer

描述 description	50S ribosomal protein L2
分子量 formula_weight	30425.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MGIRKYKPTTPGRRGASVSDFAEITRSTPEKSLVRPLHGKGGRNAHGRITTRHKGGGHKRAYRVIDFRRHDKDGVNAKVAHIEYDPNRTANIALLHYLDGEKRYIIAPQGLKQGDVIESGANADIKPGNNLPLRNIPAGTVIHAVELRPGGGAKLARSAGVSIQLLGKEGTYAALRMPSGEIRRVDVRCRATVGEVGNAEQSNINWGKAGRMRWKGKRPTVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGKPEGRTRKPNKPSDKLIVRRRRTGKKR

Entity 7 polymer

描述 description	50S ribosomal protein L3
分子量 formula_weight	23011.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MARKGILGTKLGMTQVFDENNKVVPVTVVKAGPNVVTRIRTTERDGYSAVQLAYGEISPRKVIKPVAGQFAAAGVNPRRHVAELRLDDEAAVAEYEVGQELTAEIFSDGAYVDVTGTSKGKGFAGTMKRHGFRGQGAAHGAQAVHRRPGSIGGCATPGRVFKGTRMSGRMGNDRVTTQNLKVHKVDAENGVLLIKGAIPGRNGGLVVVRSAIKRGEK

Entity 8 polymer

描述 description	50S Ribosomal Protein L4
分子量 formula_weight	22920.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MTLKVDVKTPAGKTDGSVELPAELFDVEPNIALMHQVVTAQLAAKRQGTHSTKTRGEVSGGGKKPYRQKGTGRARQGSTRAPQFTGGGTVHGPKPRDYSQRTPKKMIAAALRGALSDRARNDRIHAVTELVEGQTPSTKSAKTFLGTLTENKKVLVVIGRTDEVGAKSVRNLPGVHVISPDQLNTYDVLNADDVVFSVEALNAYISANSKEGASV

Entity 9 polymer

描述 description	50S Ribosomal Protein L5
分子量 formula_weight	21152.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

MTTTEKALPRLKQRYREEIREALQQEFNYANVMQIPGVVKVVVNMGVGDAARDAKLINGAINDLALITGQKPEVRRARKSIAQFKLREGMPIGARVTLRGDRMWEFLDRLISIALPRIRDFRGLSPKQFDGTGNYTFGLNEQSMFHEIDVDSIDRPRGMDITVVTTATNDAEGRALLRALGFPFKEN

Entity 10 polymer

描述 description	50S ribosomal protein L6
分子量 formula_weight	19483.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

MSRIGKQPVPVPSGVDVTINGQNLSVKGPKGTLTLDVAEPISVSRAEDGAIVVTRPDDERRSRSLHGLSRTLIANLVTGVTEGYTQKMEIFGVGYRVQLKGQNLEFALGYSHPVLIEAPEGITFAVESPTKFSVSGIDKQKVGQISAVIRRLRRPDPYKGKGVRYEGEQIRRKVGKTGK

Entity 11 polymer

描述 description	50S ribosomal protein L9
分子量 formula_weight	15949.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H

MKLILTAEVEHLGAAGDTVEVKDGYGRNYLLPRGLAIVASRGAERQAEEIRRARESKVIRDIEHANELKTALEGLGDVTLSVNAAGDTGKLFGSVTAADVVNAIKKAGGPNLDKRTVQLAKAHIKSVGTHPVTVKLHTGVEAKVSLNVVAQ

Entity 12 polymer

描述 description	50S ribosomal protein L10
分子量 formula_weight	18035.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	I

MAKADKATAVADIAEQFKASTATVVTEYRGLTVANLAELRRALGDSATYTVAKNTLVKRAASEAGIEGLDELFAGPTAIAFVKGEAVDAAKAIKKFAKDNKALVIKGGYMDGKALSVADVEKIADLESREVLLAKLAGAMKGNLSKAAGLFNAPASQVARLAAALQEKKAGEEAA

Entity 13 polymer

描述 description	50S ribosomal protein L11
分子量 formula_weight	15023.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J

MAPKKKVAGLIKLQIQAGQANPAPPVGPALGQHGVNIMEFCKAYNAATESQRGNVIPVEITVYEDRSFTFALKTPPAAKLLLKAAGVQKGSGEPHKTKVAKVTWDQVREIAETKKADLNANDIDAAAKIIAGTARSMGITVE

Entity 14 polymer

描述 description	50S Ribosomal Protein L13
分子量 formula_weight	16146.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K

MPTYTPKAGDTTRSWYVIDASDVVLGRLASAAATLLRGKHKPTFTPNVDGGDFVIVINADKIAVSGDKLTKKFAYRHSGYPGGLRKRTIGELLEKHPTRVVENAIIGMLPHNKLGRQIQKKLKVYAGPDHPHAAQQPIPFEIKQVAQ

Entity 15 polymer

描述 description	50S ribosomal protein L14
分子量 formula_weight	13400.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

MIQQESRLKVADNTGAKEILCIRVLGGSSRRYAGIGDVIVATVKDAIPGGNVKRGDVVKAVVVRTVKERRRADGSYIKFDENAAVIIKNDNDPRGTRIFGPVGRELREKKFMKIVSLAPEVL

Entity 16 polymer

描述 description	50S ribosomal protein L15
分子量 formula_weight	15602.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

MSVIKLHDLKPAPGEKKAKTRVGRGEGSKGKTAGRGTKGTKARKNVPVMFEGGQMPIHMRLPKLKGFKNRFRTEYQVVNVGDINKAFPQGGTVGVDELVAKGLVRKNSLVKVLGDGKLTVKVDVTANKFSGSAREAITAAGGSATEL

Entity 17 polymer

描述 description	Large ribosomal subunit protein uL16
分子量 formula_weight	15774.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

MLIPRKVKHRKQHHPEQRGIASGGTSVSFGDYGIQALEHAYITNRQIESARIAINRHIKRGGKVWINIFPDRPLTKKPAETRMGSGKGSPEWWVANVKPGRVLFELSYPDEKTARDALTRAIHKLPIKARIVTREEQF

Entity 18 polymer

描述 description	50S ribosomal protein L17
分子量 formula_weight	21892.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

MPKPTKGPRLGGSSSHQSALLANLATSLFEHGRIKTTEPKARALRPYAEKLITHAKKGALHNRREVMKKIRDKDVVHTLFAEIGPFYADRNGGYTRIIKVENRKGDNAPMAVIELVREKTVTDEANRARRAAASQAKADERADEKADEKAEETVEETTEAPAEESTEAAAEETVEETTEAPAEESTEAAEESEAKDDTK

Entity 19 polymer

描述 description	50S Ribosomal Protein L18
分子量 formula_weight	13711.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P

MAHKPVGQNISEVRRNARLRRHARLRKKVAGTAEVPRLVVNRSARHIHVQLVNDLNGTTLAAASSIEADVRAIDGDKKAHSVRVGQLIAERAKAAGVETVVFDRGGYTYGGRIAALADAAREAGLKF

Entity 20 polymer

描述 description	50S ribosomal protein L19
分子量 formula_weight	12892.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q

MNTLDFVDQASLRDDIPTFSPGDTVNVHVKVIEGSKERIQVFKGVVIRRQGGGISETFTVRKESYGVGVERTFPVHSPNIDHIDVLTRGDVRRAKLYYLRELRGKKAKIKEKR

Entity 21 polymer

描述 description	50S Ribosomal Protein L20
分子量 formula_weight	14494.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R

MARVKRALNAQKKRRTVLKASKGYRGQRSRLYRKAKEQQLHSLTYAYRDRRARKGEFRKLWISRINAAARANDITYNRLIQGLKAAGVEVDRKNLAELAVSDPAAFTALVDVARAALPEDVNAPSGEAA

Entity 22 polymer

描述 description	50S Ribosomal Protein L21
分子量 formula_weight	11055.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S

MATYAIVKTGGKQYKVAAGDVVKVEKLDSEPGASVSLPVALVVDGANVTSKADDLAKVAVTAEVLEHTKGPKIRIHKFKNKTGYHKRQGHRQQLTVLKVTGIK

Entity 23 polymer

描述 description	50S Ribosomal Protein L22
分子量 formula_weight	16353.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T

MSTVTEFPSATAKARYVRVSATKARRVIDLVRGKSVEEALDILRWAPQAASEPVAKVIASAAANAQNNEGLDPSTLVVATVYADEGPTAKRIRPRAQGRAFRIRKRTSHITVIVESRPPKQKGASAASARSRRAQGSKAAATKKSAETKEGSE

Entity 24 polymer

描述 description	50S Ribosomal Protein L23
分子量 formula_weight	11047.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	U

MATITDPRDIILAPVISEKSYGLIEDNVYTFVVHPDSNKTQIKIAIEKIFDVKVDSVNTANRQGKRKRTRTGFGKRKSTKRAIVKLAAGSKPIDLFGAPA

Entity 25 polymer

描述 description	50S ribosomal protein L24
分子量 formula_weight	11228.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	V

MKVHKGDTVLVISGKDKGAKGKVLVAYPDRNKVLVEGVNRIKKHTAVSANERGASSGGIVTQEAPIHVSNVMVVDSDGKPTRVGYRIDDETGKKVRIAKTNGKDI

Entity 26 polymer

描述 description	50S ribosomal protein L25
分子量 formula_weight	22571.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	W

MAKTANIPNKLTANVRTRTGKGASRQARRDGKVPAVLYGHGTDPQHLELNARDFAAVLRSHGTNAILTLDIEGTEQLALTKALDVHPIRRNIQHADLLVVQRGEKVTVEVTVLVEGDATPGTLVTQDANTIEIEAEALSIPEQLTVSVEGVEAGTQITAGQISLPEGVNLISDPELLVVNVVEAPSAEALEEEGAGETGAAEAPAEEAPAEEPAE

Entity 27 polymer

描述 description	50S ribosomal protein L27
分子量 formula_weight	9249.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	X

MAHKKGASSSRNGRDSAAQRLGVKRFGGQVVKAGEILVRQRGTHFHPGVNVGRGGDDTLFALAPGAVEFGAKRGRKTVNIVPVARPEA

Entity 28 polymer

描述 description	50S Ribosomal Protein L28
分子量 formula_weight	6891.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Y

MAAVCDICGKGPGFGKSVSHSHRRTSRRWNPNIQPVRAVTRPGGNKQRINACTSCIKAGKVSRA

Entity 29 polymer

描述 description	50S ribosomal protein L29
分子量 formula_weight	8790.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Z

MAVGTTPGELRELTDDELKDKLRESKEELFNLRFQMATGQLSNNRRLRTVRQEIARVYTVLRERELGLASGPAGEES

Entity 30 polymer

描述 description	50S ribosomal protein L32
分子量 formula_weight	6467.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	b

MAVPKRRMSRANTRSRRAQWKAEAPGLVTVSVAGQQRKVPRRLLKAARLGLVDLDKR

Entity 31 polymer

描述 description	50S Ribosomal Protein L33
分子量 formula_weight	6468.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	c

MASSTDVRPKITLACEVCKHRNYITKKNRRNDPDRLEIKKFCPNCGTHQPHKESR

Entity 32 polymer

描述 description	50S ribosomal protein L34
分子量 formula_weight	5522.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	d

MAKGKRTFQPNNRRRARVHGFRLRMRTRAGRAIVANRRSKGRRALTA

Entity 33 polymer

描述 description	50S ribosomal protein L35
分子量 formula_weight	7298.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	e

MPKAKTHSGASKRFRRTGTGKIVRQKANRRHLLEHKPTKRTRRLDGRTTVSAADNSRINKLLNG

Entity 34 polymer

描述 description	50S ribosomal protein L36
分子量 formula_weight	4341.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	f

MKVNPSVKPICDKCRVIRRHGRVMVICSDPRHKQRQG

Entity 35 polymer

描述 description	50S Ribosomal Protein L31
分子量 formula_weight	8312.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	g

MKTGIHPEYVDTTVQCGCGHSFTTRSTKQSGTIVVEVCSQCHPFYTGKQKILDSGGRVARFEKRYGKRNKAAADK

Entity 36 non-polymer

描述 description	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
分子量 formula_weight	521.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.09 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	84008

fit	/app/data/saxs_computation/vk/8vk7/8vk7.fit
pddf	/app/data/pr_computation/vk/8vk7/8vk7.pr.out
profile	/app/data/saxs_computation/vk/8vk7/8vk7.dat

Structure of Mycobacterium smegmatis 50S ribosomal subunit bound to HflX:50S-HflX-B

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (36)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 polymer

聚合体 Polymer

Entity 34 polymer

聚合体 Polymer