8w9z

The cryo-EM structure of the Nicotiana tabacum PEP-PAP

Method: ELECTRON MICROSCOPY D_max: 266.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8w9z
沉积日期 deposition_date	2023-09-06
结构标题 title	The cryo-EM structure of the Nicotiana tabacum PEP-PAP
关键词 keywords	TRANSCRIPTION, PEP, PAP; TRANSCRIPTION
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	77.40 Å
回转半径 Rg (电子) rg_electron	76.72 Å
零角强度 I(0) i0	10570700000.00
分子量 molecular_weight	856930.0 kDa
排除体积 excluded_volume	1064100 Å³
包络体积 envelope_volume	1856000 Å³
水化壳体积 shell_volume	198580 Å³
包络直径 envelope_diameter	270.8 Å
壳层 Rg shell_rg	77.21 Å
包络 Rg envelope_rg	75.62 Å
形状 Rg shape_rg	76.82 Å
总 Rg total_rg	76.39 Å
总原子数 total_atoms	60525
残基数 n_residues	8235
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	266.8 Å
Rg (实空间) rg_real	80.57 Å
Rg 误差 (实空间) rg_real_error	1.41 Å
I(0) (实空间) i0_real	1.0570e+10
I(0) 误差 (实空间) i0_real_error	2.1210e+08
Rg (倒空间) rg_reciprocal	77.42 Å
I(0) (倒空间) i0_reciprocal	10570000000.0000
解质量估计 total_estimate	0.8993
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	93.4 Å
偏度 Skewness skewness	0.452
峰度 Kurtosis kurtosis	-0.102
角度范围 angular_range	— – 0.1000 Å⁻¹
当前正则化参数 α current_alpha	1.1970
最高正则化参数 α highest_alpha	740400000.0000
实空间数据点数 n_real_points	21
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.893; Stabil: 0.880; Sysdev: 1.000; Positv: 1.000; Valcen: 0.982; Smooth: 0.411

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (21)

Entity 1 polymer

描述 description	DNA-directed RNA polymerase subunit alpha
分子量 formula_weight	38663.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,a
原子数 atom_count	60525

MVREKVTVSTRTLQWKCVESRTDSKRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRVKSEKVPHEYSTITGIQESVHEILMNLKEIILRSNLYGTSDASICVKGPGSVTAQDIILPPYVEIVDNTQHIASLTEPIDFCIGLQIERNRGYLIKTPHNFQDGSYPIDAVFMPVRNANHSIHSYGNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFIPFLHMEEDNLYLQDNQHTVPLSPFTFHDKLAKLIKNKKKIALKSIFIDQSELPSRIYNCLKMSNIYTLLDLLNNSQEDLMKIEHFRSEDVKRILGILEKYFVIDLAKNKF

Entity 2 polymer

描述 description	DNA-directed RNA polymerase subunit beta
分子量 formula_weight	120698.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

MLGDGNEGISTIPGFNQIQFEGFCRFIDQGLTEELYKFPKIEDTDQEIEFQLFVETYQLVEPLIKERDAVYESLTYSSELYVSAGLIWKNSRDMQEQTIFIGNIPLMNSLGTSIVNGIYRIVINQILQSPGIYYRSELDHNGISVYTGTIISDWGGRSELEIDRKARIWARVSRKQKISILVLSSAMGLNLREILENVCYPEIFLSFLSDKERKKIGSKENAILEFYQQFACVGGDPVFSESLCKELQKKFFQQRCELGRIGRRNMNRRLNLDIPQNNTFLLPRDILAAADHLIGLKFGMGALDDMNHLKNKRIRSVADLLQDQFGLALVRLENVVRGTICGAIRHKLIPTPQNLVTSTPLTTTYESFFGLHPLSQVLDRTNPLTQIVHGRKLSYLGPGGLTGRTASFRIRDIHPSHYGRICPIDTSEGINVGLIGSLAIHARIGHWGSLESPFYEISERSTGVRMLYLSPGRDEYYMVAAGNSLALNQDIQEEQVVPARYRQEFLTIAWEQVHLRSIFPFQYFSIGASLIPFIEHNDANRALMSSNMQRQAVPLSRSEKCIVGTGLERQAALDSGALAIAEREGRVVYTNTDKILLAGNGDILSIPLVIYQRSNKNTCMHQKLQVPRGKCIKKGQILADGAATVGGELALGKNVLVAYMPWEGYNSEDAVLISERLVYEDIYTSFHIRKYEIQTHVTSQGPEKVTNEIPHLEAHLLRNLDKNGIVMLGSWVETGDILVGKLTPQVVKESSYAPEDRLLRAILGIQVSTSKETCLKLPIGGRGRVIDVRWIQKRGGSSYNPETIRVYILQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGRSVDMVFNPLGVPSRMNVGQIFECSLGLAGSLLDRHYRIAPFDERYEQEASRKLVFSELYEASKQTANPWVFEPEYPGKSRIFDGRTGNPFEQPVIIGKPYILKLIHQVDDKIHGRSSGHYALVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPNPEDAPESFRLLVRELRSLALELNHFLVSEKNFQINRKEA

Entity 3 polymer

描述 description	DNA-directed RNA polymerase subunit gamma
分子量 formula_weight	79367.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MNNNFSSMIDRYKHQQLRIGSVSPQQISAWATKILPNGEIVGEVTKPYTFHYKTNKPEKDGLFCERIFGPIKSGICACGNYRVIGDEKEDPKFCEQCGVEFVDSRIRRYQMGYIKLACPVTHVWYLKRLPSYIANLLDKPLKELEGLVYCDFSFARPITKKPTFLRLRGLFEYEIQSWKYSIPLFFTTQGFDTFRNREISTGAGAIREQLADLDLRIIIENSLVEWEELGEEGHTGNEWEDRKVGRRKDFLVRRVELAKHFIRTNIEPEWMVLCLLPVLPPELRPIIQIDGGKLMSSDINELYRRVIYRNNTLTDLLTTSRSTPGELVMCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIVVGPSLSLHRCGLPREIAIELFQTFVIRGLIRQHLASNIGVAKSKIREKEPIVWEILQEVMQGHPVLLNRAPTLHRLGIQAFQPVLVEGRAICLHPLVCKGFNADFDGDQMAVHVPLSLEAQVEARLLMFSHMNLLSPAIGDPISVPTQDMLIGLYVLTSGNHRGICVNRYNPCNRRNYQNQKRSDNSHYKYTKEPFFSNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASRETPIEVHYESLGTFYEIYGHYLIVRSLKKQILFIYIRTTVGHIALYREIEEAIQGFSRAYSSGT

Entity 4 polymer

描述 description	;DNA-directed RNA polymerase subunit beta'' ;
分子量 formula_weight	157057.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	c

MAERANLVFHNKAINGTAMKRLISRLIDHFGMAYTSHILDQVKTLGFQQATATSISLGIDDLLTIPSKGWLVQDAEQQSLILEKHHHYGNVHAVEKLRQSIEIWYATSEYLRQEMNPNFRMTDPFNPVHIMSFSGARGNASQVHQLVGMRGLMSDPQGQMIDLPIQSNLREGLSLTEYIISCYGARKGVVDTAVRTSDAGYLTRRLVEVVQHIVVRRTDCGTARGISVSPRNGMMPERIFIQTLIGRVLADDIYMGPRCIATRNQDIGIGLVNRFITFRAQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVELGEAVGIIAGQSIGEPGTQLTLRTFHTGGVFTGGTAEHVRAPSNGKIKFNEDLVHPTRTRHGHPAFLCSIDLYVTIESEDILHNVNIPPKSLLLVQNDQYVESEQVIAEIRAGISTLNFKEKVRKHIYSDSDGEMHWSTDVYHAPEFTYGNVHLLPKTSHLWILLGRPCRSSLVYLSIHKDQDQMNAHFLSGKRRYTSNLSVTNDQARQKLFSSDFSGKKEDRIPDYSDLNRIICAGQYNLVYSPILHENSDLLSKRRRNKFIIPLHSIQELENELMPCSGISIEIPVNGIFRRNSILAYFDDPRYRRKSSGIIKYGTVETHSVIKKEDLLEYRGVKEFRPKYQMKVDRFFFIPEEVHILPGSSSIMVRNNSIVGVDTQITLNLRSRVGGLVRVERKKKRIELKIFSGDIHFPGETDKISRHTGVLIPPGTGKRNSKESKKVKNWIYVQRITPSKKKFFVLVRPVVTYEITDGINLATLFPPDPLQERDNVQLRIVNYILYGNGKPIRGISDTSIQLVRTCLVLNWNQDKKSSSCEEARASFVEIRTNGLIRHFLRINLVKSPISYIGKRNDPSGSGLLSDNGSDCTNINPFSSIYSYSKAKIQQSINQPQGTIHTLLNRNKECQSLIILSAANCSRMGPFKDVKYHSVIKKSIKKDPLIPIRNSLGPLGTSLPIENFYSSYHLITHNQILVTNYLQLDNLKQTFQVIKFKYYLMDENGKIFNPDPCRNIILNPFNLNWYFLHHNYCEETSKIISLGQFICENVCIAKNGPPLKSGQVILVQVDSIVIRSAKPYLATPGATVHGHYGETLYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSVDSISMNLEKRIEGWNKCITRILGIPWGFLIGAELTIAQSRISLVNKIQQVYRSQGVQIHNRHLEIIVRQITSKVLVSEDGMSNVFSPGELIGLLRAERMGRALEEAICYRVVLLGITRASLNTQSFISEASFQETARVLAKAALRGRIDWLKGLKENVVLGGVIPVGTGFKGLVHPSKQHNNIPLETKKKNLFEGEMRDILFHHKKLFDSCLSKNFHDIPEQSFIGFNDS

Entity 5 polymer

描述 description	PAP1(pTAC3)
分子量 formula_weight	101413.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MAGISIHYLPFNSKFTLPIPRPSISRSIKASVSSQPRKTRRRKQTQQQQHQLKTAEDDGSMASGTEKVLRLVFMEELMERARNADSKGVSQVIYDMIAAGLSPGPRSFHGFVVSHVLNRDNDGAMHALRRELSEGLRPLHETFLALVRLFGAKGLATRGLEILAAMEKLKYDIRQAWLVLVEELVRSNHLEDANKVFLKGAEGGLRATDEIYDLLIEQDCKVGDHSNALTIAYEMEAAGRMATTFHFNCLLSVQATCGIPEIAFATFENMEYGEDHMKPDTETYNWVIQAYTRAESYDRVQDVAELLGMMVEDHKRVQPNVRTYALLVECFTKYCVVREAIRHFRGLKNFEGGTQVLYNDGKYGDPLSLYLRALCREGRIVELLEALEAMAKDNQPIPPRAMILSRKYRTLVSSWIEPLQEEAELGYEIDYIARYVAEGGLTGDRKRWVPRRGKTPLDPDAEGFIYSNPRETSFKQRCLEEWRLHHRKLLKTLHNEGPSILGKISESDYIRLVERLRKIIKGPEQNALKPKAASKMIVSELKEELEAQGLPTDGTRNVLYQRVQKARRINRSRGRPLWVPPVEEEEEEVDEELDELISRIKLHEGNTEFWKRRFLGEGLNENHVQQSEIIDLEPTDVVDDTDDAVDDISKEAEDDEAEDDEAQDEEEEVEQAESQPEVGDRKDKEVEAAKPLQMIGVQLLKDSDQTASSSRKSRRRLSRVAAVDDDDDDWFPLDIQEAFVEMRKRNIFDVSDMYTITDAWGWTWEKEIKNKAPQRWSQEWEVELGIKVMTKVIELGGTPTIGDCAVILRAAVRAPMPSAFLNILQTTHSLGYVFGSPLYDEIITLCLDLGELDAAIAIVADLETSGIKVPDETLDRVISARQSSDTPVNGSQ

Entity 6 polymer

描述 description	Pentatricopeptide repeat-containing protein At1g74850, chloroplastic-like
分子量 formula_weight	96666.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MSLSCNSFSPVFTPVPPSHRFLFPAKIPNYGKLSPVHRRLLLTVAVRAKPKELILGNPTVTVEKGKYSYDVETLINKLSSLPPRGSIARCLDTFKNKLSLTDFSHVFKEFAARGDWQRSLRLFKYMQRQIWCKPNEHIYTLMIGILGREGLLDKAFEIFDEMPSHSVARTVFSYTAIINAYGRNGQYGTSLQLLEKMKQEKIIPSILTYNTVINSCARGGHEWEGLLSLFAEMRHEGIQPDLVTYNTLLSACSSRGLGDEAEMVFRTMNEAGILPDVTTYSYLVDTFGKLGKLEKVSELLMEMEAGGTSPEVTSYNVLLEAYAHSGSMKEAMDVFRQMQTAGCVANAETYSVLLNLYGKNGRYDQVRDLFLEMKMSNTEPDADTYNILIQVFGEGGYFKEVVTLFHDMVEEKVEPNMETYEGLIYACGKGGLHDDAKRILLHMNGQGLVPSSKVYTGVIEAYGQAALYEEAVVAFNTMNEVGSRPMVETFNSLIHAFAKGGLYKESEAIWFRMGEVGVPRNRDSFNGMIEGYRQGGQFEEAIKAYVEMEKARCDPDERTLEAVLSVYCFAGLVDESEEQFQEIKSLGIQPSIICCCMMLAIYAKSERWNMAHELLNDVMTNKTSDMHQVIGQMIHGDFDDENNWQMVEYVFDKLNSEGCGLSMRFYNTLIEALWWLGQKERAARVLNEATKRGLFPELFRRNKLVWSVDVHRMWPGGACTAISVWLNDMEELFHKGEELPQLASVVVVRGQTEKSSITRDFPVAKAAYSFLKDTVSSSFCFPGWNKGRIVCQKTQLKRIFSSAEPSSGSSKGDRLIPLSNSPISLLGTQTSVSDAKRSESANADSKRSTKSDSELMASSV

Entity 7 polymer

描述 description	Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10-like
分子量 formula_weight	80403.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

MQTLQSSSLFFTFPSSKTLLKPQSSKFSSFSLFPSSLSKPSRPLTLHCFSSDEFPVGDDDAFLEAFGPKEKESEEEARRKNWVDRGWAPWEEILSPEANFARKSLNEGEEVALQSPEAIEAFKMLSPNYRKKKISDMGITEDEYYAKQFEIKGEIPEPLSTMWAGPLVVRHVPPRDWPPRGWEVDKKELEFIRETHKLQSVRVDYDKVEEMVKMETDDMGLDRYKMFLKQYNEWVAANKDRLEKESYKYDQDYYPGRRKRGKDYQDGMYELPFYYPGQICAGKVTAIHLYQGAFVDIGGVHDGWVPIKRNDWYWIRHHIKVGMHVIVEILAKRDPYRFRFPIEMRFIDPNIDHLIFNRFDFAPIFHRDEDTNLDELRRDCGRQPLPRKDPGVKVEEEPLLSNHPYVDKLWQIHNAEQMILDDMEANPVKYKGKNLTELTDDEDFDEENRIEYSKAYYKKALLPKMITKVSVKELDLEAAFAERQHHNKLRMEAQERGEVYKIPKLRRNIEMDEYDFIHWRRSLEEREAMLRDISCRRALGLPLEEPGRYVDPSAFGKDQYDPDSPLYRYDYWGEPKNSEKSKQERMTDVHNKSIVGKGTVWYEMAYEDAVKERMQMEAQGIVRELYDEDSDSDEVGTDDDDDDEEDFDYSILGDPSANVSNQPYVNGTESRLSDEGMFEDKS

Entity 8 polymer

描述 description	superoxide dismutase
分子量 formula_weight	30420.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

MSWSSCNSLCTSSSRQLLVTTDLSSQLNIPSRLSNVKRSLHGHHGVPKVFAYYGLKTPPYKLDALEPYMSQKMLEVHWGEHHRSYVEALNKQLEKNDILYGCTMEELIKVTYNNGNPLPEFSDAAQVWNHDFFWESMQPGGGDMPKLGLLQQIEKDFGSFTNFREKFTEAALALFGSGWIWLVLKREEKRLAIVKTSNAVNPLVWNDIPLIGLDLWEHAYYLDYKNDKAKYVNVFMNHLVSWDAALGRMARAQAFVNLGEPKIPVA

Entity 9 polymer

描述 description	Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12-like
分子量 formula_weight	61281.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H

MASVLSTSLYQDRGLRTMVSTDGFVPSFCCPYRKTLFTGTIPVSIWQFKLVSSSPFQKAPLAPCIKCENKEKDQASQGSFEQVSVERYPYHSYMDSTSGQLEPASGARASIPGQEYWPEGTASRVRAARAPEPTGTSTGTPSYGKNPGSRRKKYKASAAASKPSEINIISDDSAESPDNLPEEPKDLSSEYVIYQPEQEEEELTGYELDKRLGRPHPFIDPKTKKKIEKPLTSEELWWNWRKPEKEQWSRWQRRRPDVETVFLKAMAETGQVKLYGDHPTLTETALYRARRHLYKKERLQAEKEKLEKIGPIAYYSEWVQAWKKDTSREAIQKHFEETGEDENTQLIEMFCHQTDREYRIMMGTDIRIPRDPLAMRMREDQIKQIWGGDPVYPTINYIQDPDEVIDYRGPDFHEPTPNMLAYLKEHGKIISREELEKILAKEKTEEIEVAEIDEAMARAVDIGENDDEEEGSDAEVEEGDEKITRNWSVLKSNPELRKSKEKPKKKDMSLEEAVDDSENLTDFLMDFDEDE

Entity 10 polymer

描述 description	Fructokinase-like 1, chloroplastic
分子量 formula_weight	55770.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	I

MASIHFLPQSLPHHSHNSLTHFNFSSIFFSSKLPHRKNTLKCSSNDHSTTTQEPPKHSPRGPKKSSRTAKKTSKPQKDSNFETNIEKSNTQMELSKNADDKIEKYDDGVDFPYPNPPLVICFGAAQKEFVPTVRVSHEQMHQDKYSEWKMLQWNPPEFVRAPGGPPSNVAISHVRLGGRAAFMGKVGNDEFGQEMVLLMNKEKVQTRAVKFDDSMRTGCTHMKIKFEDGKMKVEKVKEPAEDSLSSSELNLDVLKEARIFHFNSEVLTSSSMRSTLFKAISLSKKFGGLVFFDLNLPLPLWRSRDETRNLIKEAWEQANIIEVSRQELEFLLDEEHYERKRNYRPQYFAENFEQTKQRRDYYHYTPAEIAPLWHDGLKLLFVTDGTLRLHYYSPSFDGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTQPEMYHDQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW

Entity 11 polymer

描述 description	Fructokinase-like 2, chloroplastic
分子量 formula_weight	72534.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	i

MAALSFSLLSTLPRQHLHWNFYPNMKVMQLQDLGLKNKWVLMAVSKEGTPEAIAKELSKTEVFGAGKKTVRISKRAPVMARRKKVVETSNDDPVNVEEAENTSGSTEEPKKTQRRTRKKKEIVESSFGDSISDAEGNVIDAEAVTEPESSAKPKKTRRTRKKKETVVSTFEDSTLDVEGNVTDEEVLPTSGSSEGSVEIRKRTSKKAASSSSSLEKEPTQKVTRRRRKKVNNLEDEGSQTELSDIEEELHVANADADSEEELDFDGGEDISFSYGWPPLVCCFGAAQHAFVPSGRPSNRLVDHEWHERMKDAIWDPEKFTRAPGGCSSNVAVALASLGGKVAFMGKLGDDDFGQSLVYFMNINKVQTRSVRLDSKKATAITHMKIGKRGGLRMTTTKPSAEDSLLKSEINIDVLKEAKMFYFNTFSMLDPNMRLTTLRATKISKKLGGVVFYDVNLPFPLWESGDKAKTFIQQAWDLADIIEVTKQELEFLCGIKPSERFDTKDNDRSKFTHYPPEVIAPLWHENLKVLFVTNGTSKIHYYTKEHNSAVLGLEDVPLTPYTSDMSASGDGIIAGIIRMLTVQPHLMTDKGYLERTLKYAISCGVVDQWLQARRLGYPPKEGMEDDVVPDDHGIKSVTEREYRILVPVS

Entity 12 polymer

描述 description	Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14-like isoform X2
分子量 formula_weight	58183.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J

MVSSTILIQQPTNLFHQPEFQHQLWRQGCAAPLQKQPIIICSSNKGRSRPLRVTASANGAATSSTLEGYDSSPTPSAFPLFTPPSQPHDTPASQLELADPDFYKIGYVRSFRAYGIEFREGPDGYGVFASRDVEPLRRARVIMEIPLELMLTISKKLPWMFFPDIIPVGHPIFDIINSTNPETDSDLRLACLLLYAFDCKDNFWQLYGDFLPSDDECTSFLLATEEDLLELQDEKLASTMREQQQRALEFWEKNWHSAVPLKIKRLARDPERFIWAMCIAQSRSINLQMRIGALVQDANLLVPYADMMNHSFQPNCFFHWRFKDRMLEVMINAGQRIRKGDEMTVDYMAGQKNNFFMQRYGFSSPVNPWDVIHFTGDAKIHLDTFLSVFNISGLPGEYYHNSRLSNDGDSFVDGAIIAAARTLPTWSDGDLPPIPSLERKAVKELQEECHQMLAEFPTTSDEDQKILDSMPDCRRTLEAAIKYRLHRKLLIEKVIQALDIYQDRILF

Entity 13 polymer

描述 description	PAP8(pTAC6)
分子量 formula_weight	38014.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K

MSAAQLFFPLPPNLSTFSTTPSSQALFTISFAKTISSNPNSVKQSLTTKRRRDFRVFADDGDADGGGPDDYDMDEDEVEEADNKKDFDVDYDTLLGGASLTVAATGDDIAMVHSSSFVFTQGWDSEKIVDYRINEEEFHKISLLDCDFFIRKPPDPDNDVYDFREMYVTPPDTDIYAIPRVLAPMPQKYIRCAMSDYGCYNVTEPPIDAPRDPMYKSEREVSKVFLTKHYRNRRAGDPEFALDFEEIYVIDSKTKSITRAKVVVTVPGGRNRDRKNDLLVIRDNGTSFKIIPSEERDDPTTVIEKEEWKKSRQDMERHLRKLRDFSVSNWF

Entity 14 polymer

描述 description	superoxide dismutase
分子量 formula_weight	34825.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

MMAATASTTLTSAFLPFQGFRESCRTLNWRTHKKQFARKAGPVKVTAKFELMPPPYPMNALEPHMSHTTFEYHWGKHHRAYVDNLNKQIDGTELDGMTLEDIILITYNRGDLLPPFNNAAQAWNHQFFWESMKPSGGGKPSGELLQLINRDFGSFEAFVKEFKAAAATQFGSGWAWLAYKANRLNVGNTSNPHPTDEDKKLVVVKTPNAVNPLVWDYSPLLTIDVWEHAYYLDFRNRRPDYISIFMEKLVSWEAVSSRLEVAKAKAAEREEEEERKKREKEEEYKAGGEVREMYVESTDSDAE

Entity 15 polymer

描述 description	Thioredoxin-like protein CITRX1, chloroplastic
分子量 formula_weight	20142.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M,m

MQAATLSFHPLAPPPQTSACHFSSNQRKYSLFSYTCPTPRPSLLSTQTLSRKSICKPPAVATGKYVREDYLVKKVSAKDIQELIKGERNVPLIIDFYATWCGPCILMAQELEMLAVEYESNALIVKVDTDDEYEFARDMQVRGLPTLYFISPDPNKDAIRTEGLIPIQMMRDIINNDL

Entity 16 polymer

描述 description	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase-like
分子量 formula_weight	85288.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

MPFTLFSLPPFPSLLHHNPPPLQFKPFTSLHHLRLKYPTTLTTVSAIGADGKYYPNPSDDDPPEAPEDSMHGVNKFQQIQRQAAKAKKQQEELFKKEQSIFVNALADVEDAPDNPALNDNDNSGDDLFGEIDKAIALKRKEFVKQGLLKPNPPKNSTLVESEVENVDELEPEEVVDLEEIDGLSGLAEIEESDEEKSDFEVSDDMGKSEFSDLSSFDIDFDEFGKTKPRIVEPKFRMSLAELLDESRVVPLSVYGDLEVGISGVQHDSRLVESGDLFVCCVGRKTDGHLYLSEADKRGAVAVVASKEIDIEETLGCKALVIVEDTNAVLAVLAASFYRHPSKSMTLIGITGTNGKTTTSYLIKAMYEAMGLRTGMLSTVGYYIYGDNKLESPHTTPDAVLVQKLMAKMVHNGTEALVMEASSHGLALGRCDKVDFDIAVFTNLTRDHLDFHGTEEEYRDAKAKLFARMVDPARHRKIVNIDDPNAAFFLAQGNPDVPIVTFAMENKSADVHPLKFQLSLFETQVLVNTPQGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVKGIEEVDAVPGRCELIDEEQAFGVIVDYAHTPDALSRLLDYVRELGPRRVITVFGCAGESDRGKRPIMAKIATDKSDVTILTSDNPKTEDPLDILDDMLAGVGWSMQDYLKHGENDYYPPLPNGHRLFLHDIRRVAVRCAVAMGEEGDIVVVAGKGHETYQIEGDKKEFFDDREECREALQYVDELHQAGIDTSEFPWRLPESH

Entity 17 polymer

描述 description	Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7-like isoform X1
分子量 formula_weight	19254.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

MAASTLTFSGFSTLQTFPKIAAVENTKRVNFSIRSQSASNSKNESRGGRRIWRRRKLTKKDETLDAKMERIPFLEEQVRKIRDGGKLLTMDIHRLLLNEDNRFDFVNEIAAEAKQYVENNRDEYGAKKAILHVLSNRMNDAGVYRAEAYMESDPFKPGPGYMKDELL

Entity 18 polymer

描述 description	PAP13(pTAC18)
分子量 formula_weight	17054.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P

MASFITMPALSYLSTNTSSLERTNFRPSSRGYQGVRAMRTEKPLEELYNVRVERNVTKDRLMELGVPRWSMWKTGKCKLPWDWHVDQLVYIEEGEVRVVPEGSQRFMQFVAGDLVRYPKWFEADLYFNDFYQERYSFRAYGDS

Entity 19 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 20 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 21 non-polymer

描述 description	FE (III) ION
分子量 formula_weight	55.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	triclinic

分辨率 resolution	3.00 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	410910

fit	/app/data/saxs_computation/w9/8w9z/8w9z.fit
pddf	/app/data/pr_computation/w9/8w9z/8w9z.pr.out
profile	/app/data/saxs_computation/w9/8w9z/8w9z.dat

The cryo-EM structure of the Nicotiana tabacum PEP-PAP

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (21)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 non-polymer

Entity 20 non-polymer

Entity 21 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)