8ww8

Structure of Apo-Form AsfvPrimPol Hexamer

Method: ELECTRON MICROSCOPY D_max: 188.7 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8ww8
沉积日期 deposition_date	2023-10-25
结构标题 title	Structure of Apo-Form AsfvPrimPol Hexamer
关键词 keywords	VIRAL PROTEIN, DNA BINDING PROTEIN; DNA BINDING PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	60.57 Å
回转半径 Rg (电子) rg_electron	59.96 Å
零角强度 I(0) i0	4458930000.00
分子量 molecular_weight	578040.0 kDa
排除体积 excluded_volume	728270 Å³
包络体积 envelope_volume	1128500 Å³
水化壳体积 shell_volume	152650 Å³
包络直径 envelope_diameter	192.9 Å
壳层 Rg shell_rg	66.48 Å
包络 Rg envelope_rg	57.71 Å
形状 Rg shape_rg	59.95 Å
总 Rg total_rg	60.15 Å
总原子数 total_atoms	40886
残基数 n_residues	5129
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	188.7 Å
Rg (实空间) rg_real	60.69 Å
Rg 误差 (实空间) rg_real_error	0.57 Å
I(0) (实空间) i0_real	4.3410e+09
I(0) 误差 (实空间) i0_real_error	7.1030e+07
Rg (倒空间) rg_reciprocal	60.90 Å
I(0) (倒空间) i0_reciprocal	4464000000.0000
解质量估计 total_estimate	0.6959
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	79.8 Å
偏度 Skewness skewness	0.180
峰度 Kurtosis kurtosis	-0.272
角度范围 angular_range	— – 0.1300 Å⁻¹
当前正则化参数 α current_alpha	1.7280
最高正则化参数 α highest_alpha	515200000.0000
实空间数据点数 n_real_points	27
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.889; Stabil: 0.925; Sysdev: 0.000; Positv: 1.000; Valcen: 0.945; Smooth: 0.666

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (1)

Entity 1 polymer

描述 description	Putative primase C962R
分子量 formula_weight	112721.5 kDa
来源方法 src_method	man
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F
原子数 atom_count	40886

MREESWEDHDTIQLTAQRKYLAEVQALETLLTRELSVFLTEPGSKKTNIINRITGKTYALPSTELLRLYEHLEQCRKQGALMYFLERQGTYSGLMLDYDLKLNTNAVPPLEPPALSRLCHRIFVHIKNSSVLPEGSHKIHFFFTLKPEVVQGKYGFHVLIPGLKLAASTKKSIIGSLQHDATVQKILHEQGVTNPESCLDPHSASVPSLLYGSSKLNHKPYQLKTGFELVFDSSDPDYIPIHQIKNLESYNLVSELSLTNEQGSLVRPVYCAADIAAEKEEEIPTEDHSLSILMLHDPEARYLHKILNLLPPEYYVEYPLWSNVVFALANTSANYRPLAEWFSQKCPEKWNTGGKEKLEKLWNDASHHTEKKITKRSIMYWAHKHAPQQYKEIVEQGYFSILAEYVYSYNGMLEHYMIAKVIYAMMGNKFVVDVDSNGKYVWFEFVLPGQPMNQGEIWKWRKEVNPDELHIYISENFSRVMDRITEHIKYHLSQPHESNILNYYKKLLKAFERSKSKIFNDSFKKGVIRQAEFLFRQRSFIQTLDTNPHLLGVGNGVLSIETIPAKLINHFHEHPIHQYTHICYVPFNPENPWTKLLLNALQDIIPELDARLWIMFYLSTAIFRGLKEALMLLWLGGGCNGKTFLMRLVAMVLGDHYASKLNISLLTSCRETAEKPNSAFMRLKGRGYGYFEETNKSEVLNTSRLKEMVNPGDVTARELNQKQESFQMTATMVAASNYNFIIDTTDHGTWRRLRHYRSKVKFCHNPDPSNPYEKKEDPRFIHEYIMDPDCQNAFFSILVYFWEKLQKEYNGQIKKVFCPTIESETEAYRKSQDTLHRFITERVVESPSAETVYNLSEVVTAYAEWYNTNINVKRHIALELSQELENSVLEKYLQWSPNKTRILKGCRILHKFETLQPGESYIGVSTAGTLLNTPICEPKNKWWEWSPNPSAPPEKEASAPTPLEDYKDDDDK

来源 Source

来源生物 organism	African swine fever virus
NCBI 分类编号 ncbi_taxonomy_id	10497
宿主生物 host_organism	Homo sapiens

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7. 引用文献 (1)

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分辨率 resolution	3.99 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	33986

fit	/app/data/saxs_computation/ww/8ww8/8ww8.fit
pddf	/app/data/pr_computation/ww/8ww8/8ww8.pr.out
profile	/app/data/saxs_computation/ww/8ww8/8ww8.dat