8x9n

Cte_A695U_Branch Intermediate,Mg,inactive

Method: ELECTRON MICROSCOPY D_max: 150.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8x9n
沉积日期 deposition_date	2023-11-30
结构标题 title	Cte_A695U_Branch Intermediate,Mg,inactive
关键词 keywords	CP group ii intron, Cte, back-splicing, circular RNA, cryo-EM, SPLICING, RNA; RNA, SPLICING
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	45.47 Å
回转半径 Rg (电子) rg_electron	45.21 Å
零角强度 I(0) i0	2549220000.00
分子量 molecular_weight	238840.0 kDa
排除体积 excluded_volume	222660 Å³
包络体积 envelope_volume	426730 Å³
水化壳体积 shell_volume	78463 Å³
包络直径 envelope_diameter	155.7 Å
壳层 Rg shell_rg	49.84 Å
包络 Rg envelope_rg	44.32 Å
形状 Rg shape_rg	45.18 Å
总 Rg total_rg	45.36 Å
总原子数 total_atoms	15736
残基数 n_residues	730
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	150.2 Å
Rg (实空间) rg_real	45.36 Å
Rg 误差 (实空间) rg_real_error	1.72 Å
I(0) (实空间) i0_real	2.5490e+09
I(0) 误差 (实空间) i0_real_error	4.8370e+07
Rg (倒空间) rg_reciprocal	45.47 Å
I(0) (倒空间) i0_reciprocal	2550000000.0000
解质量估计 total_estimate	0.8809
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	51.5 Å
偏度 Skewness skewness	0.289
峰度 Kurtosis kurtosis	-0.358
角度范围 angular_range	— – 0.1750 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	109000000.0000
实空间数据点数 n_real_points	36
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.846; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.916

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (5)

Entity 1 polymer

描述 description	RNA (219-mer)
分子量 formula_weight	70596.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	E
原子数 atom_count	15744

GCCGGGGCGCCACCCCGGAAGUGAUGCGAGUCGCAACUCGCAUCACAAGCAAACGCUGUAGCCGCGUGCCUCUAAUAGGGCUGGCGCGGUUGCGAAGGGCGCUGGUGAGUGCAACUCUCACCUUCGACCCAAUCCAUCUUGCGGCUCAACCCCGCAAGAUCAUCGCCAGACCGCUGGCGGCGUACUGAGUGACAAACGAGGCAAAACCAAAUUGAAGUU

Entity 2 polymer

描述 description	RNA (589-mer)
分子量 formula_weight	191687.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	A

GGGCGACCGUGAAACGGCGCUGGGCAGGAAAUGGCCCAGUGACCUGGUCAAUGGUGAAAAUCGGUGAAAGACCGACCGGUGGGGCGUAUCGAAAGAGCGCAACGCCUGCCGCACAGGAUGGCUUCUGAGGUACCGGUGACGGUACAGAACGCGGAGGGGAAACCUGGAAGCGAGGGCACCUCGGGAAACCGGGGGUCGAUGCAUAGCUCAAACCUGUAACGGCACCAGUGAAGGGUGCUGUGCGGAGCAACGUGGAGCCACAGGCAUGAAGCCGUGGUUCGUAGUCGAUGAGACAAGCGGUGAGUAAGGGAAGGGCUGCGAACAUCGCCUCCCCGAAAUCCAAGGAAAGCCGAAAGGCUAGCCGCUUUGUUGAGACAGUGGCGCCACGUUGCGCAUUAGCCGUGACCUAAACGGGGAACCUCUUGGCCGUACCGACUCGGGUGGCACCGGUCGGGCUCGAUGGCUCAAGAGGGGGUGAUGUGAUGAUUAGGGUUUGACCCGUGAUGCGAUACGACCGAAGCAUCCGGGGAGCUGUCUGACGAAGAGUCGGCAGCAGUGGGUUUGGCGACCCGCUCCGAAAGUCGCAAGC

Entity 3 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	15

Entity 4 non-polymer

描述 description	POTASSIUM ION
分子量 formula_weight	39.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	13

Entity 5 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	8

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	triclinic

分辨率 resolution	3.01 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	74584

fit	/app/data/saxs_computation/x9/8x9n/8x9n.fit
pddf	/app/data/pr_computation/x9/8x9n/8x9n.pr.out
profile	/app/data/saxs_computation/x9/8x9n/8x9n.dat

Cte_A695U_Branch Intermediate,Mg,inactive

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (5)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)