8yp6

Cryo-EM map of 30S ribosomal subunit in complex with MetAP1c of Mycobacterium smegmatis

Method: ELECTRON MICROSCOPY D_max: 211.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8yp6
沉积日期 deposition_date	2024-03-15
结构标题 title	Cryo-EM map of 30S ribosomal subunit in complex with MetAP1c of Mycobacterium smegmatis
关键词 keywords	Methionine aminopeptidase type1c, anti-association factor, inter-subunit face, RIBOSOME; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	67.62 Å
回转半径 Rg (电子) rg_electron	68.10 Å
零角强度 I(0) i0	18108300000.00
分子量 molecular_weight	761550.0 kDa
排除体积 excluded_volume	796290 Å³
包络体积 envelope_volume	1289100 Å³
水化壳体积 shell_volume	157560 Å³
包络直径 envelope_diameter	238.4 Å
壳层 Rg shell_rg	68.49 Å
包络 Rg envelope_rg	66.30 Å
形状 Rg shape_rg	68.09 Å
总 Rg total_rg	68.11 Å
总原子数 total_atoms	51445
残基数 n_residues	3940
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	211.6 Å
Rg (实空间) rg_real	67.68 Å
Rg 误差 (实空间) rg_real_error	1.32 Å
I(0) (实空间) i0_real	1.8100e+10
I(0) 误差 (实空间) i0_real_error	3.6540e+08
Rg (倒空间) rg_reciprocal	67.18 Å
I(0) (倒空间) i0_reciprocal	18090000000.0000
解质量估计 total_estimate	0.8411
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	73.6 Å
偏度 Skewness skewness	0.417
峰度 Kurtosis kurtosis	-0.344
角度范围 angular_range	— – 0.1150 Å⁻¹
当前正则化参数 α current_alpha	0.0011
最高正则化参数 α highest_alpha	867000000.0000
实空间数据点数 n_real_points	24
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.946; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.093

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (20)

Entity 1 polymer

描述 description	16S rRNA
分子量 formula_weight	489828.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	a
原子数 atom_count	51445

UGGAGAGUUUGAUCCUGGCUCAGGACGAACGCUGGCGGCGUGCUUAACACAUGCAAGUCGAACGGAAAGGCCCUUUCGGGGGUACUCGAGUGGCGAACGGGUGAGUAACACGUGGGUGAUCUGCCCUGCACUUUGGGAUAAGCCUGGGAAACUGGGUCUAAUACCGAAUACACCCUGCUGGUCGCAUGGCCUGGUAGGGGAAAGCUUUUGCGGUGUGGGAUGGGCCCGCGGCCUAUCAGCUUGUUGGUGGGGUGAUGGCCUACCAAGGCGACGACGGGUAGCCGGCCUGAGAGGGUGACCGGCCACACUGGGACUGAGAUACGGCCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCGACGCCGCGUGAGGGAUGACGGCCUUCGGGUUGUAAACCUCUUUCAGCACAGACGAAGCGCAAGUGACGGUAUGUGCAGAAGAAGGACCGGCCAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGGUCCGAGCGUUGUCCGGAAUUACUGGGCGUAAAGAGCUCGUAGGUGGUUUGUCGCGUUGUUCGUGAAAACUCACAGCUUAACUGUGGGCGUGCGGGCGAUACGGGCAGACUAGAGUACUGCAGGGGAGACUGGAAUUCCUGGUGUAGCGGUGGAAUGCGCAGAUAUCAGGAGGAACACCGGUGGCGAAGGCGGGUCUCUGGGCAGUAACUGACGCUGAGGAGCGAAAGCGUGGGGAGCGAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGGUGGGUACUAGGUGUGGGUUUCCUUCCUUGGGAUCCGUGCCGUAGCUAACGCAUUAAGUACCCCGCCUGGGGAGUACGGCCGCAAGGCUAAAACUCAAAGGAAUUGACGGGGGCCCGCACAAGCGGCGGAGCAUGUGGAUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGGUUUGACAUGCACAGGACGCCGGCAGAGAUGUCGGUUCCCUUGUGGCCUGUGUGCAGGUGGUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUGUCUCAUGUUGCCAGCACGUUAUGGUGGGGACUCGUGAGAGACUGCCGGGGUCAACUCGGAGGAAGGUGGGGAUGACGUCAAGUCAUCAUGCCCCUUAUGUCCAGGGCUUCACACAUGCUACAAUGGCCGGUACAAAGGGCUGCGAUGCCGUGAGGUGGAGCGAAUCCUUUCAAAGCCGGUCUCAGUUCGGAUCGGGGUCUGCAACUCGACCCCGUGAAGUCGGAGUCGCUAGUAAUCGCAGAUCAGCAACGCUGCGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACGUCAUGAAAGUCGGUAACACCCGAAGCCGGUGGCCUAACCCUUGUGGAGGGAGCCGUCGAAGGUGGGAUCGGCGAUUGGGACGAAGUCGUAACAAGGUAGCCGUACCGGAAGGUGCGGCUGGAUCA

Entity 2 polymer

描述 description	Small ribosomal subunit protein uS3
分子量 formula_weight	23805.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	c

MGQKINPHGFRLGITTEWKSRWYADKQYKDYVKEDVAIRKLLATGLERAGIADVEIERTRDRVRVDIHTARPGIVIGRRGTEADRIRADLEKLTGKQVQLNILEVKNPESQAQLVAQGVAEQLSNRVAFRRAMRKAIQSAMRQPNVKGIRVQCSGRLGGAEMSRSEFYREGRVPLHTLRADIDYGLYEAKTTFGRIGVKVWIYKGDIVGG

Entity 3 polymer

描述 description	Small ribosomal subunit protein uS4
分子量 formula_weight	23284.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	d

ARYTGPATRKSRRLGVDLVGGDQSFEKRPYPPGQHGRARIKESEYRQQLQEKQKARFSYGVMEKQFRRYYEEANRQPGKTGDNLLRILESRLDNVVYRAGLARTRRMARQLVSHGHFLVNGVKVDIPSYRVSQYDIIDVKEKSLNTLPFQIARETAGERPIPSWLQVVGERQRILVHQLPERAQIDVPLTEQLIVELYSK

Entity 4 polymer

描述 description	Small ribosomal subunit protein uS5
分子量 formula_weight	20443.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	e

RGRRDDRGGRGRDGGDKSNYIERVVSINRVSKVVKGGRRFSFTALVIVGDGKGMVGVGYGKAKEVPAAIAKGVEEARKNFFRVPLIGSTITHPVQGEAAAGVVMLRPASPGTGVIAGGAARAVLECAGVHDILAKSLGSDNAINVVHATVAALKLLQRPEEVAARRGLPIEDVAPAGMLKARRESEALAAAAAREGSA

Entity 5 polymer

描述 description	Small ribosomal subunit protein bS6
分子量 formula_weight	10991.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	f

MRPYEIMVILDPTLDERTVAPSLETFLNVIRKDGGTVDKVDIWGRRRLAYEIAKHAEGIYAVIDVKAEPATVSELDRQLNLNESVLRTKVLRTDKH

Entity 6 polymer

描述 description	30S ribosomal protein S7
分子量 formula_weight	17660.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	g

MPRKGPAPKRPLVNDPVYGSQLVTQLVNKVLLEGKKSLAERIVYGALEQAREKTGTDPVVTLKRALDNVKPALEVRSRRVGGATYQVPVEVRPDRSTTLALRWLVNFSRQRREKTMVERLANEILDASNGLGASVKRREDTHKMAEANRAFAHYRW

Entity 7 polymer

描述 description	Small ribosomal subunit protein uS8
分子量 formula_weight	14260.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	h

MTDPIADFLTRLRNANSAYHDEVTLPHSKLKANIAEILKREGYISDYRTEDARVGKSLVVQLKYGPSRERSIAGLRRVSKPGLRVYAKSTNLPRVLGGLGVAIISTSSGLLTDRQAARQGVGGEVLAYVW

Entity 8 polymer

描述 description	Small ribosomal subunit protein uS9
分子量 formula_weight	14093.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	i

PIQTVGRRKEAVVRVRLVPGTGQFNLDGRTLENYFPNKVHQQLIKAPLVTVDRVDQFDIYAHLDGGGPSGQAGALRLAIARALILVQPEDRPALKKAGFLTRDPRAIERKKYGLKKARKAPQYSKR

Entity 9 polymer

描述 description	Small ribosomal subunit protein uS10
分子量 formula_weight	11066.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	j

KIRIRLKAYDHEAIDASARKIVETVTRTGASVVGPVPLPTEKNVYCVIRSPHKYKDSREHFEMRTHKRLIDILDPTPKTVDALMRIDLPASVDVNIQ

Entity 10 polymer

描述 description	Small ribosomal subunit protein uS11
分子量 formula_weight	12380.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	k

KKNVPHGAAHIKSTFNNTIVSITDPQGNVIAWASSGHVGFKGSRKSTPFAAQLAAENAARKAQEHGVKKVDVFVKGPGSGRETAIRSLQAAGLEVGTISDVTPQPHNGCRPPKRRRV

Entity 11 polymer

描述 description	Small ribosomal subunit protein uS12
分子量 formula_weight	13678.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	l

PTIQQLVRKGRRDKIAKVKTAALKGSPQRRGVCTRVYTTTPKKPNSALRKVARVKLTSQVEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGSLDTQGVKNRKQARSRYGAKKEK

Entity 12 polymer

描述 description	Small ribosomal subunit protein uS14B
分子量 formula_weight	6845.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	n

AKKALVHKANKKPKFAVRAYTRCNKCGRPHSVYRKFGLCRICLREMAHAGELPGVQKSSW

Entity 13 polymer

描述 description	Small ribosomal subunit protein uS15
分子量 formula_weight	10079.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	o

ALTAEQKKEILGQYGLHDTDTGSPEAQVALLTKRIQDLTEHLKVHKHDHHSRRGLLLLVGRRRRLLKYVAQVDVARYRSLIERLGLR

Entity 14 polymer

描述 description	Small ribosomal subunit protein bS16
分子量 formula_weight	12635.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	p

AVKIKLTRLGKIRNPQYRIIVADARTRRDGRAIEVIGRYHPKEEPSLIQIDSERAQYWLGVGAQPTEPVLALLKITGDWQKFKGLPGAEGTLKVKEPKPSKLDLFNAALAEAE

Entity 15 polymer

描述 description	Small ribosomal subunit protein uS17
分子量 formula_weight	10423.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	q

GPKYTPAAEKPRGRRKTAIGYVVSDKMQKTIVVELEDRKSHPLYGKIIRTTKKVKAHDENGEAGIGDRVSLMETRPLSATKRWRLVEILEKA

Entity 16 polymer

描述 description	Small ribosomal subunit protein bS18B
分子量 formula_weight	7338.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	r

KTRKCVFCSKKGQTIDYKDTALLRTYISERGKIRARRVTGNCVQHQRDIAVAVKNAREVALLPF

Entity 17 polymer

描述 description	Small ribosomal subunit protein bS20
分子量 formula_weight	9353.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	t

NIKSQIKRIRTNERRRLRNQSVKSSLRTAIRGFREAVDAGDKDKASELLHATSRKLDKAASKGVIHPNQAANKKSALALALNKL

Entity 18 polymer

描述 description	Small ribosomal subunit protein uS19
分子量 formula_weight	9167.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	s

KKGPFVDDHLLKKVDVQNEKNTKQVIKTWSRRSTIIPDFIGHTFAVHDGRKHVPVFVTEAMVGHKLGEFAPTRTFKGHIK

Entity 19 polymer

描述 description	Methionine aminopeptidase
分子量 formula_weight	32354.4 kDa
EC 编号 ec_number	3.4.11.18
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A

MSVRSALRPGVLSPTLPVPKSIPRPEYAWKPTVQEGSEPWVQTPEVIEKMRVAGRIAAGALAEAGRAVAPGVTTDELDRIAHEYMIDHGAYPSTLGYKGFPKSCCTSLNEIICHGIPDSTVIEDGDIVNIDVTAYIDGVHGDTNATFLAGDVSEEHRLLVERTHEATMRAIKAVKPGRALSVVGRVIEAYANRFGYNVVRDFTGHGIGTTFHNGLVVLHYDQPAVETVLEPGMTFTIEPMINLGTLDYEIWDDGWTVATTDGKWTAQFEHTLVVTEDGAEILTQLAAALQHHHHHH

来源 Source

来源生物 organism	Mycolicibacterium smegmatis MC2 155
NCBI 分类编号 ncbi_taxonomy_id	246196
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 20 polymer

描述 description	Small ribosomal subunit protein uS13
分子量 formula_weight	13445.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	m

ARLVGVDLPRDKRMEIALTYIYGIGRTRSNEILAATGIDKNMRTKDLTDDQVTVLRDYIEGNLKVEGDLRREVQADIRRKIEIGCYQGLRHRRGLPVRGQRTKTNARTRKGPKRTIA

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	4.70 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	50296

fit	/app/data/saxs_computation/yp/8yp6/8yp6.fit
pddf	/app/data/pr_computation/yp/8yp6/8yp6.pr.out
profile	/app/data/saxs_computation/yp/8yp6/8yp6.dat

Cryo-EM map of 30S ribosomal subunit in complex with MetAP1c of Mycobacterium smegmatis

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (20)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

来源 Source

Entity 20 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)