9awj

Bovine adult muscle nAChR bound to ACh

Method: ELECTRON MICROSCOPY D_max: 154.1 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9awj
沉积日期 deposition_date	2024-03-05
结构标题 title	Bovine adult muscle nAChR bound to ACh
关键词 keywords	Bovine muscle nicotinic acetylcholine receptor, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	45.00 Å
回转半径 Rg (电子) rg_electron	44.26 Å
零角强度 I(0) i0	752557000.00
分子量 molecular_weight	241120.0 kDa
排除体积 excluded_volume	307570 Å³
包络体积 envelope_volume	406650 Å³
水化壳体积 shell_volume	76889 Å³
包络直径 envelope_diameter	163.5 Å
壳层 Rg shell_rg	48.82 Å
包络 Rg envelope_rg	44.51 Å
形状 Rg shape_rg	44.28 Å
总 Rg total_rg	44.42 Å
总原子数 total_atoms	34036
残基数 n_residues	2041
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	154.1 Å
Rg (实空间) rg_real	45.23 Å
Rg 误差 (实空间) rg_real_error	1.59 Å
I(0) (实空间) i0_real	7.5260e+08
I(0) 误差 (实空间) i0_real_error	1.3320e+07
Rg (倒空间) rg_reciprocal	45.00 Å
I(0) (倒空间) i0_reciprocal	752300000.0000
解质量估计 total_estimate	0.8364
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	48.4 Å
偏度 Skewness skewness	0.561
峰度 Kurtosis kurtosis	-0.048
角度范围 angular_range	— – 0.1750 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	116400000.0000
实空间数据点数 n_real_points	36
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.725; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.974; Smooth: 0.721

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (13)

Entity 1 polymer

描述 description	Acetylcholine receptor subunit alpha
分子量 formula_weight	49949.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,C
原子数 atom_count	34041

SEHETRLVAKLFEDYNSVVRPVEDHRQAVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLDYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVVINPESDQPDLSNFMESGEWVIKESRGWKHWVFYACCPSTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPEWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAEEWKYVAMVMDHILLAVFMLVCIIGTLAVFAGRLIELNQQG

Entity 2 polymer

描述 description	Acetylcholine receptor subunit beta
分子量 formula_weight	55111.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

SEAEGRLREKLFSGYDSTVRPAREVGDRVWVSIGLTLAQLISLNEKDEEMSTKVYLDLEWTDYRLSWDPEEHEGIDSLRISAESVWLPDVVLLNNNDGNFDVALDINVVVSSDGSMRWQPPGIYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLQTGLSPEGQERQEVYIHEGTFIENGQWEIIHKPSRLIQPSVDPRGGGEGRREEVTFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKMGLSIFALLTLTVFLLLLADKVPETSLSVPIIIKYLMFTMVLVTFSVILSVVVLNLHHRSPHTHQMPLWVRQIFIHKLPLYLGLKRPKPERDQMQEPPSIAPRDSPGSGWGRGTDEYFIRKPPNDFLFPKPNRFQPELSAPDLRRFIDGPNRAVGLPPELREVVSSISYIARQLQEQEDHDVLKEDWQFVAMVVDRLFLWTFIIFTSVGTLVIFLDATYHLPPADPFP

Entity 3 polymer

描述 description	Acetylcholine receptor subunit delta
分子量 formula_weight	56543.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

LNEEERLIRHLFEEKAYNKELRPAAHKESVEISLALTLSNLISLKEVEETLTTNVWIEQGWTDSRLQWDAEDFGNISVLRLPADMVWLPEIVLENNNDGSFQISYSCNVLIYPSGSVYWLPPAIFRSSCPISVTYFPFDWQNCSLKFSSLKYTTKEITLSLKQAEEDGRSYPVEWIIIDPEGFTENGEWEIVHRPARVNVDPSVPLDSPNRQDVTFYLIIRRKPLFYVINILVPCVLISFMINLVFYLPADCGEKTSMAISVLLAQSVFLLLISKRLPATSMAIPLIGKFLLFGMVLVTMVVVICVIVLNIHFRTPSTHVLSEPVKKLFLETLPEILHMSRPAEDGPSPGTLIRRSSSLGYISKAEEYFSLKSRSDLMFEKQSERHGLARRLTTARRPPAGSEQAQQELFSELKPAVDGANFIVNHMKDQNNYNEEKDCWNRVARTVDRLCLFVVTPIMVVGTAWIFLQGAYNQPPPQPFPGDPFSYLEKDKRFI

Entity 4 polymer

描述 description	Acetylcholine receptor subunit epsilon
分子量 formula_weight	52613.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

KNEELRLYHYLFDTYDPGRRPVQEPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKGDFGGVETLRVPSELVWLPEIVLENNIDGQFGVAYEANVLVSEGGYLSWLPPAIYRSTCAVEVTYFPFDWQNCSLVFRSQTYNAEEVEFVFAVDDEGKTISKIDIDTEAYTENGEWAIDFCPGVIRRHDGDSAGGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKTPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHAMSPRLRYVLLELLPQLLGSGAPPEIPRAASPPRRASSLGLLLRAEELILKKPRSELVFEQQRHRHGTWTATLCQNLGAAAPEIRCCVDAVNFVASSTRDQEATGEEVSDWVRMGKALDSICFWAALVLFLVGSSLIFLGAYFNRVPQLPYPPCM

Entity 5 branched

描述 description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
分子量 formula_weight	1721.5 kDa
来源方法 src_method	man
拷贝数 entity_count	1

Entity 6 branched

描述 description	;alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
分子量 formula_weight	748.7 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 7 branched

描述 description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
分子量 formula_weight	1559.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 8 branched

描述 description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
分子量 formula_weight	1235.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 9 branched

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	424.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

Entity 10 branched

描述 description	;alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
分子量 formula_weight	910.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 11 non-polymer

描述 description	ACETYLCHOLINE
分子量 formula_weight	146.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 12 non-polymer

描述 description	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate
分子量 formula_weight	760.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

Entity 13 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	5

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.45 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	167900

fit	/app/data/saxs_computation/aw/9awj/9awj.fit
pddf	/app/data/pr_computation/aw/9awj/9awj.pr.out
profile	/app/data/saxs_computation/aw/9awj/9awj.dat

Bovine adult muscle nAChR bound to ACh

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (13)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 branched

Entity 6 branched

Entity 7 branched

Entity 8 branched

Entity 9 branched

Entity 10 branched

Entity 11 non-polymer

Entity 12 non-polymer

Entity 13 water

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)