9b41

Pseudomonas phage Pa193 Neck (portal and head-to-tail proteins)

Method: ELECTRON MICROSCOPY D_max: 189.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9b41
沉积日期 deposition_date	2024-03-20
最后修订 last_revision	2024-10-16
结构标题 title	Pseudomonas phage Pa193 Neck (portal and head-to-tail proteins)
关键词 keywords	;phage, bacteriophage, gene product (gp19), STRUCTURAL PROTEIN, VIRAL PROTEIN, gene product 28 (gp28), head-to-tail protein, portal protein ;; VIRAL PROTEIN
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	59.51 Å
回转半径 Rg (电子) rg_electron	58.86 Å
零角强度 I(0) i0	8725750000.00
分子量 molecular_weight	787320.0 kDa
排除体积 excluded_volume	982260 Å³
包络体积 envelope_volume	1352600 Å³
水化壳体积 shell_volume	177600 Å³
包络直径 envelope_diameter	190.8 Å
壳层 Rg shell_rg	69.93 Å
包络 Rg envelope_rg	58.03 Å
形状 Rg shape_rg	58.87 Å
总 Rg total_rg	59.01 Å
总原子数 total_atoms	55608
残基数 n_residues	7080
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	189.2 Å
Rg (实空间) rg_real	59.15 Å
Rg 误差 (实空间) rg_real_error	1.78 Å
I(0) (实空间) i0_real	8.7260e+09
I(0) 误差 (实空间) i0_real_error	1.9160e+08
Rg (倒空间) rg_reciprocal	59.80 Å
I(0) (倒空间) i0_reciprocal	8735000000.0000
解质量估计 total_estimate	0.8668
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	80.0 Å
偏度 Skewness skewness	0.156
峰度 Kurtosis kurtosis	-0.365
角度范围 angular_range	— – 0.1300 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	953000000.0000
实空间数据点数 n_real_points	27
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.854; Stabil: 0.997; Sysdev: 1.000; Positv: 1.000; Valcen: 0.936; Smooth: 0.776

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	gp19 Portal
分子量 formula_weight	84478.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	12

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L,A,B,C,D,E,F,G,H,I,J,K
原子数 atom_count	55608

MFKLSWIFGRKKDNAACSESAPEKVAQIPQHDPLDPMIKLGRIRGWNVEPEKAPVIRSVKDFLEPGLSVAMDSAYGDGPTPAAKAAAGGQNPYVVPTMLQDWYNSQGFIGYQACAIISQHWLVDKACSMSGEDAARNGWELKSDGRKLSDEQSALIARRDMEFRVKDNLVELNRFKNVFGVRIALFVVESDDPDYYEKPFNPDGITPGSYKGISQIDPYWAMPQLTAGSTADPSSEHFYEPDFWIISGKKYHRSHLVVVRGPQPPDILKPTYIFGGIPLTQRIYERVYAAERTANEAPLLAMSKRTSTIHVDVEKAIANEDAFNARLAFWIANRDNHGVKVLGTDESMEQFDTNLADFDSIIMNQYQLVAAIAKTPATKLLGTSPKGFNATGEHETISYHEELESIQEHIFDPLLERHYLLLAKSEEIDVQLEIVWNPVDSTSSQQQAELNNKKAATDEIYINSGVVSPDEVRERLRDDPRSGYNRLTDDQAETEPGMSPENLAEFEKAGAQSAKAKGEAERAEAQAGAVEGAGDPVPAAPRGTKPLAKAAEEGASEAAEPPSRPDPKAELRNLLVDLLSKLQDLDDIKAPDGVDIEHNDAPGVKRTSKPGVSGMEPSVFSSNRIVGPRDHSELQRIKVNGITTLIENPRGSIRQGKDGSWRVQMKHHYGFIKGTKGADGDEVDCFVGPNLGSKRVFVVNQVNKEGQFDEHKCMLGFNNINDAKSGYLSCFRPGWDGLGSIHEVDLPAFRRWLANGDTTKPFGGE

Entity 2 polymer

描述 description	gp28 Head-to-tail protein
分子量 formula_weight	16885.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	12

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M,N,O,P,Q,R,S,T,U,V,W,X

MVIFDEHKFRTLFPEFADPAAYPDVRLQMYFDIACEFISDRDSPYRILNGKALEACLYLLTAHLLSLSTMQVQGAAGGGVTAGGTQGGFITSATVGEVSVAKLAPPAKNGWQWWLSGTPYGQELWALLSVKAVGGFYIGGLPERRGFRKVGGTFW

▶

7. 引用文献 (1)

▶

分辨率 resolution	3.20 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	9179

fit	/app/data/saxs_computation/b4/9b41/9b41.fit
pddf	/app/data/pr_computation/b4/9b41/9b41.pr.out
profile	/app/data/saxs_computation/b4/9b41/9b41.dat

Pseudomonas phage Pa193 Neck (portal and head-to-tail proteins)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)