9b45

Pseudomonas phage Pa193 baseplate complex and tail fiber

Method: ELECTRON MICROSCOPY D_max: 240.9 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9b45
沉积日期 deposition_date	2024-03-20
最后修订 last_revision	2024-10-16
结构标题 title	Pseudomonas phage Pa193 baseplate complex and tail fiber
关键词 keywords	phage, bacteriophage, baseplate complex, STRUCTURAL PROTEIN, VIRAL PROTEIN, tail fiber; VIRAL PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	88.38 Å
回转半径 Rg (电子) rg_electron	88.58 Å
零角强度 I(0) i0	30450000000.00
分子量 molecular_weight	1481700.0 kDa
排除体积 excluded_volume	1851000 Å³
包络体积 envelope_volume	3090200 Å³
水化壳体积 shell_volume	290040 Å³
包络直径 envelope_diameter	349.4 Å
壳层 Rg shell_rg	88.98 Å
包络 Rg envelope_rg	87.07 Å
形状 Rg shape_rg	88.62 Å
总 Rg total_rg	88.44 Å
总原子数 total_atoms	104304
残基数 n_residues	13848
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	240.9 Å
Rg (实空间) rg_real	83.74 Å
Rg 误差 (实空间) rg_real_error	0.76 Å
I(0) (实空间) i0_real	2.9050e+10
I(0) 误差 (实空间) i0_real_error	5.8800e+08
Rg (倒空间) rg_reciprocal	87.56 Å
I(0) (倒空间) i0_reciprocal	30380000000.0000
解质量估计 total_estimate	0.9161
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	107.1 Å
偏度 Skewness skewness	0.282
峰度 Kurtosis kurtosis	-0.331
角度范围 angular_range	— – 0.0900 Å⁻¹
当前正则化参数 α current_alpha	0.5959
最高正则化参数 α highest_alpha	3327000000.0000
实空间数据点数 n_real_points	19
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.001; Oscil: 0.979; Stabil: 0.986; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.017

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (11)

Entity 1 polymer

描述 description	gp45-a Baseplate wedge 1
分子量 formula_weight	43189.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	12

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	j,k,l,m,n,o,q,p,r,s,t,u
原子数 atom_count	104304

MANYNYIVDTGVIVADTADVLSDVEAEFRAALGANINLAASTPQGSLVAAEAIARSSVMRNEARIANTINPNVSFGTFLDAICALMGIERGSDLSTFGYGVQVTGRSQTRISTGSRVQTPAGAIFTVLSDVTIPAGGVATIDIKSQEYGNIPLPVGNLIIIDGTIGWSGAKVIASTRVDPGSRQMSDAELKNARVNRLAIQGRNSTMAIKAYVSAVPNVTSVNVIENNTGAVQVVNGVSFTLPYAVWVCVAGNPDKQAVADALWAAHNGGTPWDYGATNNGVPVDGPNGVPVRDPASGRKYVVKWTTPIMYDGYVNVTVQQGSSSVAPEAIQNAVVNYAQGKVEGEEGLVVGASLSAFEVAGAIAREIPGIYIKLCQVACVAAGSPAPAPGDFTSEYVMSAFGQATISVGNVRVTFV

Entity 2 polymer

描述 description	gp47 Tail fiber
分子量 formula_weight	103353.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2,3,4

MITPELIPSPFAAQGDKDPIPQTSSTGFANLRDGYTPDYEISLASNNPQAKAVERKIQNQLFFIATQNAQAWQRQMAPPWFQGMPGGYEQNAEVVRVGNDGIMRRYRSMVNANASDPLSSTTWEEQPAWSAMRSNIPMPAGGPGLSSGGEVITTGRNFNDLLNGTWEFFSDSVVIASQNAPVYPASAGAAAGMLEAKSWISGSNTFCVQRYTDRVGNVAVRGLNAGAWTNWMYAVNVMALQQGRVTYGVAAGPANAYTLTLVPQLQGGLVDGMILRVKFNTMNTGASTINVSGLGAKAIVGAANFPLTGGELGQGLIAELVFDAAGDRWRILAGAPRIQVGNADQDYQAPSWKQVKDYVASQKLTEVDWADVVNKPNVAIQDTTPWFANLELSDARPFIDFHFNNNRAKDFDYRFISEADGSMAFYSRQGSAGPTQDILFSRSNVTFLQPRLDVAKNLAYIANSGPLWQNTTADQPGWKFTFAQGVDANNNAVIAVNTTNPDGSYRSQIMRWDWASTNVIFNNRPLFAGQYVPWDSGNFDPSTKLTVNATNQIAGPTGIRNTNGNTGNMNTWGSGSTTASYGNAAIQIFGKGGGEPAAIYFDNSQTGWYLGMDKDGQLKRAGWSLGNNSYVITDELNIRNHINGMAARPVWGGNEFWGPWNFNPNTKLTLGAFNDSQHTRMVNSAAKDVGIAAMTSYGDAAMSFFNYEASTPTGNRAAVISFVRNGARGVLFGLDTDNKLKWGGYSLGAVAFEIADSNNLMSLWSSHAAAPNWNGQTIWRSGNFNPDTKATLAARNTTSSPTIFSYGASGIASTGQVGALVVENNSVTNTAAAITFHSPQKYQVNFGLDADNVVKIGGGTMGGVAYPIIHSGNYNNYINQALVQVGLGGVGSYAILAVFDTSAPAASIAPGTIMDSSKLFYSSCDSTYRSSASPTGTWRCMGYVYNRDSTNGDSASLFQRVT

Entity 3 polymer

描述 description	gp34 helical bundle
分子量 formula_weight	12270.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F

MKKIPLTAVPNQAISFNAGSSYWKIRLYQNMDMMNADISRDGVIVCHGVRCFGGIPLLQYSRQYRPDYGNFVFDRDADWTLFGDGINLFYLDGAEFAEYQALATRKE

Entity 4 polymer

描述 description	gp38 Ripcord-1
分子量 formula_weight	20000.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S,T,U

MMLGIFTSLLSSRSFSIVDQNTNQLVAADLRISRVNTRFSSVGQRHMLEDGKTKMDSRTIHPMEIIVEVFCPSIDVVDQINQLLLDRDTLYKVITRGMVFERMMCTSEALNQTPDMISATPARLTFSQVLVQNPKPIMFRNAGDSSMIDRGLALAEDVVGSAGDLFDYAVNGVQNAADLF

Entity 5 polymer

描述 description	gp41 Tape measure protein
分子量 formula_weight	94380.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	a,b,c

MSSDLDEFILRYEADTARAERNLEHLQNQIRRVNSASTSGLQDLRHFADGAATELGRVVPQIDSVTSAIRGMNAQLAIGATGVALVAAGVKAFMNTRDQYNQQRIQAMDIGIAPARLEEYQRKFVRQSGGTISREQGAEMTKNLADTFRRAYRDIGRVGPEARILRMAGVDVGSFQKGMRPLNDIITDLATKMAKLKPDEISAYADALGVSRDYLSTLAKIGPAMGKVTEMTTAELQSRVQGESNIQKFNDALANLNQTFTTLENRVGEKLAPAFTKLIEIIDKIVQAIPNEVESFAKDTKSRWEDGVLGKATVGSDILSLLSPGALLGRLAAWGTRRGMEESGLIDKDKVPGAQTSEDLAKKQEDQDKATKSMKELEKLADQTTKSTNDFAVAINMFSGAVSSFANAVDERQAWAAWAGEIGRAVGMGSTAPTSRATGVYPHAIYDQSKSGAAGQVFGEPIGAQSLRNRMFSPQRKAEPVTVPSYINDIIKDASKMYNIPELDIKKLIYTESRFNARATSEAGAKGLMQLMPEIAKAYGITDVYDPRQNILGGTRLLRENLDRANGDMRLALTYYHGGLDPKNWGPRTRAYPGLVMSAPIELMEEAQRKQKAAAMTVANETFAPEGGDMDIRPYDGGRLETPDQGKKEDERREARRYDDRVVRPEIRIIDRMPDRSDGEILKMSRRQEADRADSGFRKFPNQVRGETKQNIQAQLTAGAIAQVIGVNPNQIMRREISRSDLLFGYNQAILGKQQEIKAAATEANNVFLSPAKLAEATAKVNAASREMDILRTYGEQLLKSAPERGQELTIGRIDMLVNVTGANSPEEAREIFSKQTADQLTTAIQDAQNDSATKILY

Entity 6 polymer

描述 description	gp42 Tail hub
分子量 formula_weight	32480.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	d,e,f

MKKRILRVTFNMPYGPEVIREDLDVRVRIMKAALRIQNRATMEIFGLTTQLRESLLSQFTAWKHRQRQVGREDELMIKVSVEAGYSDQGREQVSRVFVGEVAIVDIISPPPDIGIRIQCYTRQIDRTKTIRNMPPANTTFVKFVEWGANEMGLNFICDTSYNDQVLKNPGRSITVASAILASIQDMYMPDVAAFVDDDILIVKDRDKVIRPDEVTNVNSFVGIPSWSEWGVEFQCLFEPSIRVAGGVAVESLMNPSVNGNYVITALEYDLASRDRPFYIKVMGSPAA

Entity 7 polymer

描述 description	gp39 Ripcord-2
分子量 formula_weight	21667.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	V,W,X

MNSFLKSILNTPTLTIRDDVTKLPVWKSLQVKKVEIYSPASVVSKPLATKDQTEAQVYTEALDIDVKNGKIIQPVRLRINAICPDLSTVESIMNAFNDNTSTFAITSKSILADKMAIMTLDVDQSPDMLNAAEINMEFEQVEPPVLNEFDPAFPQDSPTYGVQIQSLSDANLLDLGAIGDSISSAAKSLYNRVTSYF

Entity 8 polymer

描述 description	gp46 Baseplate wedge 2
分子量 formula_weight	54629.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	v,w,x,y,z,1

MTLPAYNSDIQQALKWLHNQAPGITGLIQRKAQWYDRFSRQFWANWERDVFHLKTANPFGLMVWCIILGTPSKGFGLYPKNSSWAFGRLRQNFIYSGTQVPPPADASPGGNFYGGGNAEILNLDEIRKVLQLRYVALISNGSIAYINRMLRYIFNDDEPWDEATGLYFYLMDSTGENGPVENLAIYRKDWEGMVLLSSSPRTNHVLTSTPASDADWPGVDPAASGIPVTVETASATAPDGSATVCKLTKPAGSTAYVSAPIDGPLGSGSTVTFSFFAKAGSTRFIAIQSAADFPSRADAVFDLDSGNVISDQMLDSSVVSARMIRLENGWWRCVLTTKTVSSSFRAAYVAPAETNFSWIDSNSSAAIDVLIWGAQIELGDTPTGYLETTGAPVTMTDYVLQNAQTGTVKFTQPLPTGVEAYWTGDWKGGTAAEPARFAVGNGTQDTFTLSDPAYIGLPTSGAFKLEYRVGPALNLSPQLINLMNDRAVGIMPTCAGCDVKVIQE

Entity 9 polymer

描述 description	gp37 Baseplate tube
分子量 formula_weight	17748.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M,N,O,P,Q,R

MINVSGFGTGIVIVSASSFPMGFSLSKFADDESPISSKELEPFGYEMLYDGGLFAFDKAAPLEVSVSVIAGSEDDINLRILLNSKKGSFRFLPGIIPDMTTLVATLPDGGRTVLSNGTIIKGPAIDTIQNTGRRKGNTYTFVFGSYLGAQTARQAISNVIQSVLEVV

Entity 10 polymer

描述 description	gp35 Sheath initiator
分子量 formula_weight	12511.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G,H,I,J,K,L

MSTSTIRTGTNNDILLDDNGNMVILRDVEACAQDVRAAMLMRTGENIFDVNSGVGYFEYIFSPQKSYDDARKSIADAILSSPDVTGIEQLDIDITGEVFGVDAKVITIHGPVTTGV

Entity 11 polymer

描述 description	gp44 Tail tip
分子量 formula_weight	24201.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	g,h,i

MSNISLTSAKAPDRTRLIAALDARSRRDALDFEVMIPAQVVQYDRAENIATIQPLITWVDTEHNAVQRHQLVDIPVISMGAGGFHISFPIQQGDIGWIYAADRDTSQFLESLSMSKPNTGRIHKFEHGLFIPDVFRRYTINSEDSAAMVIQSTSGATRISIRGDNIKITAPSNVTVDTPQANFTGNVTIANTLVVNGINVNNHGHLENNPPDARTKGGMIA

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7. 引用文献 (1)

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分辨率 resolution	3.30 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	17175

fit	/app/data/saxs_computation/b4/9b45/9b45.fit
pddf	/app/data/pr_computation/b4/9b45/9b45.pr.out
profile	/app/data/saxs_computation/b4/9b45/9b45.dat

Pseudomonas phage Pa193 baseplate complex and tail fiber

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (11)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)