9bx4

Structure of influenza A RNP, 4xNP local reconstruction, class 6

Method: ELECTRON MICROSCOPY D_max: 135.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9bx4
沉积日期 deposition_date	2024-05-22
结构标题 title	Structure of influenza A RNP, 4xNP local reconstruction, class 6
关键词 keywords	Influenza, Ribonucleoprotein complex, nucleoprotein, VIRAL PROTEIN-RNA complex; VIRAL PROTEIN/RNA
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	45.33 Å
回转半径 Rg (电子) rg_electron	44.90 Å
零角强度 I(0) i0	534429000.00
分子量 molecular_weight	116550.0 kDa
排除体积 excluded_volume	114480 Å³
包络体积 envelope_volume	330860 Å³
水化壳体积 shell_volume	62119 Å³
包络直径 envelope_diameter	134.1 Å
壳层 Rg shell_rg	49.98 Å
包络 Rg envelope_rg	42.14 Å
形状 Rg shape_rg	45.00 Å
总 Rg total_rg	44.98 Å
总原子数 total_atoms	8160
残基数 n_residues	1888
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	135.6 Å
Rg (实空间) rg_real	45.07 Å
Rg 误差 (实空间) rg_real_error	0.81 Å
I(0) (实空间) i0_real	5.3440e+08
I(0) 误差 (实空间) i0_real_error	8.7510e+06
Rg (倒空间) rg_reciprocal	45.33 Å
I(0) (倒空间) i0_reciprocal	534600000.0000
解质量估计 total_estimate	0.8842
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	65.8 Å
偏度 Skewness skewness	0.011
峰度 Kurtosis kurtosis	-0.742
角度范围 angular_range	— – 0.1750 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	318000000.0000
实空间数据点数 n_real_points	36
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.957; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.625

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Nucleoprotein
分子量 formula_weight	56454.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D
原子数 atom_count	8160

MATKGTKRSYEQMETDGERQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRVDGKWRRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINDRNFWRGENGRRTRIAYERMCNILKGKFQTAAQRTMVDQVRESRNPGNAEFEDLIFLARSALILRGSVAHKSCLPACVYGSAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTKVVPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASSGQISIQPTFSVQRNLPFDRPTIMAAFTGNTEGRTSDMRTEIIRLMESARPEDVSFQGRGVFELSDEKATSPIVPSFDMSNEGSYFFGDNAEEYDN

Entity 2 polymer

描述 description	viral RNA
分子量 formula_weight	18325.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	E,F

UUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUU

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7. 引用文献 (1)

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分辨率 resolution	9.20 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	8664

fit	/app/data/saxs_computation/bx/9bx4/9bx4.fit
pddf	/app/data/pr_computation/bx/9bx4/9bx4.pr.out
profile	/app/data/saxs_computation/bx/9bx4/9bx4.dat

Structure of influenza A RNP, 4xNP local reconstruction, class 6

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)