9cuy

Bacteriophage PhiTE extended baseplate

Method: ELECTRON MICROSCOPY D_max: 203.0 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9cuy
沉积日期 deposition_date	2024-07-26
最后修订 last_revision	2025-04-16
结构标题 title	Bacteriophage PhiTE extended baseplate
关键词 keywords	;baseplate, Sheath, Tube, PhiTE, Virus, Phage, VIRAL PROTEIN, sheath initiator, tube initiator, hub, puncture apparatus, fibers, wedge, tape measure protein ;; VIRAL PROTEIN
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	78.95 Å
回转半径 Rg (电子) rg_electron	79.02 Å
零角强度 I(0) i0	11462900000.00
分子量 molecular_weight	906410.0 kDa
排除体积 excluded_volume	1132800 Å³
包络体积 envelope_volume	2046400 Å³
水化壳体积 shell_volume	215370 Å³
包络直径 envelope_diameter	317.4 Å
壳层 Rg shell_rg	78.17 Å
包络 Rg envelope_rg	77.69 Å
形状 Rg shape_rg	78.97 Å
总 Rg total_rg	79.15 Å
总原子数 total_atoms	63911
残基数 n_residues	8324
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	203.0 Å
Rg (实空间) rg_real	75.30 Å
Rg 误差 (实空间) rg_real_error	0.51 Å
I(0) (实空间) i0_real	1.1010e+10
I(0) 误差 (实空间) i0_real_error	2.0050e+08
Rg (倒空间) rg_reciprocal	78.69 Å
I(0) (倒空间) i0_reciprocal	11450000000.0000
解质量估计 total_estimate	0.9110
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	91.4 Å
偏度 Skewness skewness	0.211
峰度 Kurtosis kurtosis	-0.586
角度范围 angular_range	— – 0.1000 Å⁻¹
当前正则化参数 α current_alpha	0.1797
最高正则化参数 α highest_alpha	1859000000.0000
实空间数据点数 n_real_points	21
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.001; Oscil: 0.977; Stabil: 0.977; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.001

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (12)

Entity 1 polymer

描述 description	Wedge 2 protein
分子量 formula_weight	54432.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T,S,R,U
原子数 atom_count	63911

MAENYGLTGSGFNLPPMDDLVQETKKTFKSAFGEDFNTESNSVADKLIQIFNEREYQLWLLMGSVYYAQTMQGAEGIYLDDLLGKRGIYRLGKTRSTGTVVMTIDSSVPYNMIYSAATYTIDTDYELSSDVQVAGNIVAQLIKGTDLSVGTYRLQIQNTTDQSVKTLSLNLTATSGQPLITFFGQIKDFIVNNTILSNQDRIWIDSTEGALYIGYDTNKIMIGLSSRVDFRTNPMAGTRSISMDVRSIEPGYISRDVHSVRSINPTPGGFVDIDNLSAFIDGSDVESDNEYRIRAATSISEGKATRPAILAALLNKVEGIEKVRIFNNNTDKTNSLGIPPYRFMVVCYGGGTAEISQVLYDTIATSNNTYGDTFYDITTEDDQVERIWHTKAAARQLAIRVRYRGRPLSLTEETAIANGLATAVNGTMIAGTLYNVRLVGTVMSSTSPDRFTQVYVDIKNKGQPDSAYVNTDVTASTTQVLSLELEDVIFSQIV

Entity 2 polymer

描述 description	Tail sheath protein
分子量 formula_weight	50647.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,C,E,B,D,F

MAEYQDKVVDVEVSLGTQPIDTVGFETPMFLAMHGNFPERIRFYVSTAGMVADGFAVGSPAYQFATNAFAGNFAPQRVAIGRMSIDSSKVDFTGTTNTEQVVVNITLNKVVKAVKINVLPGNTPAQIATALADAVTADADLTGKATAVATGTYVTVTAVSPNVVSVGKGAGVYKIVNESSETVATVLPSVIAENHNWYFLATEARSDADIVAAAEFAKANYKLHIYNSTDVDAYAPENSAASVFDTLKSLSYDSLGTSDAGADVDFTEGSVIGAMAANDPSYGDSLHLKTMPGMVPFAGSDTQRSNAWSRNANIYRGLYGGGSYIEGKTSSGQYVDVIRFSHWVKFRMEESVFAYMKRRSDMGLSMKMSDEDLPVLKSVLMNNPINIGIRNGGILTGYDTENKVSYDPTIIIPKRANIPTNDLAARILRDVKVELVYNNSLHYVKIRASVVLDRPAGQSTNAQTPMSSSAVGV

Entity 3 polymer

描述 description	Tail tube protein
分子量 formula_weight	16792.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J,H,I,G

NQSKILTLQAYDPAKVLVFIGGQRVSGFAADTKIVITRNNDNISVHAGVDGEISNALSRDNTGVMTLSLQNTAKWNGYLAQWQRQANVTGLIYLPVQVEGSQGLSLNTIGWIQKQPDLSYGTEVGQMDWEIGVLDAWLSPDQIQGIAAGITGLLGL

Entity 4 polymer

描述 description	Tube initiator protein
分子量 formula_weight	31720.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K,L

MAVNNNSISPAQQAQADKRARDAAAAKRKQDTEVSNAKSREDIQYTMFVSGLRRGRLNEFERKNRTDDLAILFDSVTTHTYTKDYNKSSYAVESKAKASDHVTTQDGKFTFSGTVTDSPYLIDPRNMIDRDTDKENPMLARRPAKAIEILELIADSHQLVTLVTEDNILSNYVITSFQVDRSSEAGSSINVQVTLEEFRFKMVNKTVMARTSDPKKAKNANTGTKQTAEDGAVDDSAKQKRQTPYIGKNAETKERWENAAIGTTDFSGKPGAKLPFDPSDLMRK

Entity 5 polymer

描述 description	Hub protein
分子量 formula_weight	36545.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	X

MRAMERTYTLVIGRPVVIGEKPVNIEKFANTSKGDAYEIKDLHIEFNVKKDNSKEPNKGYVTVYNLSDEVVNYLSVNQRESLAVMLHAGYNGDEKLIFSGTVEYVEDDFPEETRTTKFILGDGTLNLTTATTARSYRKGTPVNSVLNDLIADLKLPKGRVIDFGNQTLQTSMAFTGNASQNLANLAKNTGSTFSVQDGAVYWTKEGSRFNNVMFEISEEGGMVGTPTPKQPSSSKKLIKAKAKAKSDEAEGKKPKAPSKKKKEHDIKEDVGMTVSTLLNGAILPESTVYLNTRYHKGFYKVAELTHRGGYETGDWITELGLVETRGELIK

Entity 6 polymer

描述 description	Putative baseplate assembly protein
分子量 formula_weight	26474.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Y

MAYADRYDAAFGIYISRFLRNNVHTNIRGKVVGVNYSGPSVDVQPMAYTEFPSGTTDRYPVIYDVPVLLPSGAGGKARLTMPIKPGDVVGLSFSERNEGDNNDQNTHQLFAGWAVTQIFTDGNSKAIHPDNVVLENDKAIITLKPDGDTSLQNPKVTVEALADGNVKISNGTGEFTMSPSGEIKGGNRGGSFTLQPSGQLMCNGARITTSGRIITANGVDMDDFYQKYMSHTHGGVSRGGDNTNPPN

Entity 7 polymer

描述 description	Wedge 1 protein
分子量 formula_weight	18869.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O,P

LKDVNHIHPLPDFVRGGFDYVPSDIFSGKENFEQTLRILLERLEFIDQKMVELAELRTTLNAEDAILDEIGRQLGIYRNGLNDPEYRAVIMILTGNNSKSGTRADIIATLKQLFGEDGVTTYKGYNYRLDINIFNTCMEVTDILPEIIDMLPLVTHLRVVENQGY

Entity 8 polymer

描述 description	Spacer protein
分子量 formula_weight	13055.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N,M

RTVVRTFDFELSGYPDETFRVVLDSVTYELRFMWNERDESWFMSLGDIGAQRPTITSKLTCYSDILAPYRYLDNVPDGNLYLWPLGDIRTRAGRFNIGPLKGIQMTYSSLIE

Entity 9 polymer

描述 description	Sheath initiator protein
分子量 formula_weight	18823.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	n,m

QFKDLLLDPLTGDLDFGTPGDRGMRLALTNQLSLRQRLYLRFAIWAGDWYFDETFGFPYRTFVGKKTVKAVLDGRIKSEVRQEPDVLQITDFQSTMDVVSRSYKCFFTVVTAEGEEISLAFVGEDEYQYPTPPESNVQLCGDEGVIINFKNKLYYLINFRLPK

Entity 10 polymer

描述 description	Putative tail fiber protein
分子量 formula_weight	81935.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	g,i,h,j,k,l

MSSNTDIIALLSSDADKAVDQIIEDSKRLHIVVNGTGTEQAVAEDGSLLPSVRKALIDNLYFKTPPLPWRNGGSVNEFNQLYSFTDVSGNTTWWYAPGATVSNPVVMRDSPINDGKFKVFLDKTNIADIYAPLNSPNFVGNPRVPTPAPGDNSQSIPTTGWVQSEMEGLEDLIISSMRGEFENITVRNDAILQDTYINGEFIVTSDKLAAANTEATFRRIRMVGTGTTGTAATEIAFEQNTTPPPGVSTKTNIKPFAVSTGILTVDQINGKAAQVGDVAVSTDSLNVDGYMRGDYLHLEGNNRNTAGRPQLIVDGIAEIETLRVTGTIEGVKADVDGLDIHPRSVIVDEGLTVAKDTTMTGKLTVGGTTKVGNLEVTGTLSGVAISVDGKDIKPRSVNTGNLVATGDVQISGTTTATGKITTQDLEVLGTLTANLDLSGSDLSVDNINVAQTATITDLVVTGTTTGVKGDVEGEAIAPASVAATGTVSAASMSTTGNMAVGGNLTITGDFRPASVAGQDTPRLVVQGDSDLQGDLNVSGTITGVVDLTAQDINMNSLGLAQGLTAGGGITSFSTVKGVNGVFGEGSTAPGAIGLQSMTNAKVATDLLVGEDLRVEGDTNLFGNLSVTGTITGNMNFSGKTIAPATVNATTVTATNLTVTGTIDLTDSPIVAQEITATNVEATGKMVAGKYSTTPKTANATTATFTPDGTTSIYNVNVQADTEVQAPAGLLSSGKGESIFIYFEQDTTGHAVTFSSAFVVHNAATVSNAANSITIANLVYRGTGPLIDVFLTGR

Entity 11 polymer

描述 description	Short tail fiber
分子量 formula_weight	59236.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	c,a,b,f,d,e

MAKPKDPIYVWAAADVNLPGTGRPNKSKPIDDLLAKGYDKGQKPAAEEFNYILNMSSAWVNWIVNEKFPELEAEIARLLAELENRINQQLAVIRQDIAQLRQDVADLRRYVDQKVQELKQEIQGVRNDLNKLRQDFDAAITQVNGRIDDLEPRLVPIGAVIPWPGATVPDGWLECSGQVFNTGQNPKLYSVLGRNVVPDYRGLFLRGWAHGSDANDPDAGRALGSVQGDAIRNITGYFPADIGNSNFIGRYVGGAFRDDGSLTSGDDGTRSNEVRKYSFDASREVPVAAENRPKNIAVMYIIKTDQAQSSGGNSPTAIVVSPDTITNRIGYAVKATASVLPASISGQYPISWSTQNAAVATVDGSGNISLVGPGETNIIASISTGMNVVIRVTSYSVLTSISIADPGQITVTESKMLVVTKSPSSANEPLQFLSNNSGVAAVNDAGYVIGVSAGSATITVRGTLSGVSSTRAVTIIPEQVEESVQDNRLGAVGSYIPPSPSSNVMSWTFQAPQGCALTGIIVQENESNSGDNIGGVYYKPIQKKVNGVWVTITG

Entity 12 polymer

描述 description	Putative tape measure protein
分子量 formula_weight	87898.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	W

MAASGNVVVTRTTNRVTFETDKASYARAVKQIRQVGKEWEKASDAISKPKKDPAKAYDKSAQQMRLVNKRLAETRAREEKRASERSIALARREAKAKEAIARVSAARIKQQVQQMTSPRAGMSEMKKFYQQQEREAKKANRKATIGMNAASRPMNVTRINSAPLSPRGGQGTGMVGDPNKVYNPELIAAQNRAMAGRIRSQSQAQDRKAKAEARQRAAAAARAARIDDVMAQQRIRLSSKYGRSYEGRLGRDGAGQGIQDLNRQFRAGTLSAGQYRQSIQALERQFRSAQANAGGFGAALGDIRSQLLNAGAAYGVFASGASVLKQGQFFQGMEATMSMVSDSSEEAGQRIKFVKDQAYRLGLDLKIASQGYTQMAVNSAGILSKAQNDDLFKGFSEFATASQVDPVKFQRGITAIGQMMGKGQVMAEELKGQLAEGIPGSLQVFVKAAQEAFGDTTIDVEKLMDMMQKGELKAAKILPFVGKYFAEAARKGGALPKALESNRVAMQRLSLTWIDFQNQIFQGGFGEQMTRVFRDLATILDSNGELATNLGAFFGNVIEGFWDMVTEIHDDFVLLDRIVSYYTEKLGYQGDLLKEVFDWAGYAIGIGIFVGGLNKIFKILTKIAGLRTALVGVRQAMGGAITRGGVTNGAGLGGGAGAAGTPGASRTASFLSKWKGLSKISKVGILGTLYSAGSMLNEQFIKRGDDKLADAGLDQETFQKEYGFMPKPVGLLDVFDKWFNKPRDLDAPTITAPGPGSGMAAQTGIPFPAPQKVEGDITIKIEAGELRNMVRAVVDENNQFNFNMLIQGGPN

▶

7. 引用文献 (1)

▶

分辨率 resolution	3.24 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	16480

fit	/app/data/saxs_computation/cu/9cuy/9cuy.fit
pddf	/app/data/pr_computation/cu/9cuy/9cuy.pr.out
profile	/app/data/saxs_computation/cu/9cuy/9cuy.dat

Bacteriophage PhiTE extended baseplate

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (12)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)