9e6q

Cryo-EM structure of the Pyrobaculum calidifontis 50S ribosomal subunit in complex with Dri

Method: ELECTRON MICROSCOPY D_max: 261.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9e6q
沉积日期 deposition_date	2024-10-30
结构标题 title	Cryo-EM structure of the Pyrobaculum calidifontis 50S ribosomal subunit in complex with Dri
关键词 keywords	Ribosome, Translation, Hibernation, RNA; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	70.26 Å
回转半径 Rg (电子) rg_electron	71.83 Å
零角强度 I(0) i0	72972400000.00
分子量 molecular_weight	1576200.0 kDa
排除体积 excluded_volume	1673500 Å³
包络体积 envelope_volume	2533800 Å³
水化壳体积 shell_volume	268410 Å³
包络直径 envelope_diameter	241.0 Å
壳层 Rg shell_rg	85.47 Å
包络 Rg envelope_rg	71.71 Å
形状 Rg shape_rg	71.92 Å
总 Rg total_rg	71.72 Å
总原子数 total_atoms	106594
残基数 n_residues	8150
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	261.6 Å
Rg (实空间) rg_real	72.84 Å
Rg 误差 (实空间) rg_real_error	1.43 Å
I(0) (实空间) i0_real	7.2970e+10
I(0) 误差 (实空间) i0_real_error	1.3130e+09
Rg (倒空间) rg_reciprocal	70.90 Å
I(0) (倒空间) i0_reciprocal	73090000000.0000
解质量估计 total_estimate	0.8730
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	83.2 Å
偏度 Skewness skewness	0.528
峰度 Kurtosis kurtosis	0.377
角度范围 angular_range	— – 0.1100 Å⁻¹
当前正则化参数 α current_alpha	0.8392
最高正则化参数 α highest_alpha	10420000000.0000
实空间数据点数 n_real_points	23
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.631; Stabil: 0.916; Sysdev: 1.000; Positv: 1.000; Valcen: 0.934; Smooth: 0.912

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (43)

Entity 1 polymer

描述 description	23S rRNA
分子量 formula_weight	984007.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	1
原子数 atom_count	114588

UAGGCAAAGCCGCCCGGUGGAUGGCUCGGCUCGGGCGCCGAAGAAGGGCGUGGCAAGCUGCGAUAAGCCCGGGGUAGCCGCAGGCAGGCUUAGAACCCGGGAUCCCCGAAUGGGGCUUCCUGCCGGGGCCGAAUAGGCCCCGGCGCCCCGUAAGGGGCGGGAACGCGGGGAAAGGAAACAUCUUAGUACCCGCAGGAAGGGAAGCCAACAGGGACCCCCUGAGUAGGGGCGACCGAAAGGGGGAUAGCCCAAACCAAAUCCUCGCGGGACAACCGUGGGGAGAUGUGGGGCUUGGGCCCGGGCAACCGCCGGCGGGCGGUAGCCGAAGUGGGCUGGAAUGCCCCGCCGUAGAGGGUGAUAGCCCCGUAGGCGAAACCGCCCGUGGCGGAGUCCCGGGGUCCCGGAGUACCUCGGCUUAGUUUUGCCGGGGGAACGCGCCGGCCACUGGCCGGCAAGGCUAAGCACGUCCCGAGUCCGAUAGCGCACUAGUACCGUGAGGGAAAGCUGAAAAGAACCCCGGAAGGGGGGUGAAAAGAGCCUGAAACCGGGCGGCUACAGUGGGGCAGGCCCGAAAGGAUGCCCCCUCCCGAAGGAAACCCCGGUGACGGGGGAGUACGAGGGAGGGGGUCCAGGGUCUGCCCUUACGUCUAGAAACACGGGCCGGGGAGUUCACGGCCGUGGCGAGCCUAAGGGGUUCAACCCCGGAGGCGUAGGGAAACCGACAGCCCGCAGCGGGGCAACCCGCGAGGGGCGGGGUCUUAAAGGGCCCGUAGUCACGGCCGUGAGACCAGAAACCGGGCGAUCUAGCCCUGGGCAGGGUGAAGCGGGGCGAAAGCCCCGUGGAGGCCCGAAGGGGUUCUGAUGUGCAAAUCGUUCCCAUGACCUGGGGCUAGGGGCAAAAGACCAAUCAAGCCCGGUGAUAGCUGGUUCCCCCCGAAGCGGGUCUCAGCCCGGCCUCCCCGGAGGCGGCCGGCGGGGUAGAGUACUGAUCGGGGGUGCGGGAGCCGAAAGGCUCCGGCCCCCGGUCAAACUCCGAACCUGCCAGCGCCGUAGAAGGGGGGAGGCGGGGGCGGUGGGGUAAGCCUCCGCUCCGAGACGGGAACAACCGAGACCGGGGUUAAGGCCCCCAAGUGCGGGCUUAGUGUCAAUCUAAAAGGGCGUCCCCCGCCCAAGACAGCGGGGCCGUGGGCCUAACAGCAGCCAUCGGCUAAGCAACGCGUAACAGCGGACCCGCCGAGGCGGGGGGCCCCGAAGAUGUACAGGGACUAAGCCCGCCGCCGAGACCCCGGCCCGCGGGCCGUUGGCCCGCGUGGGGUAGGGGGGCGCGGCCGUGGGGCAGAAGCCGGGCCGUGAGGUCCGGUGGACCCGCGGCCGACGAAGAUCCCGGCGGUAGUAGCAGCGAAGAGGGGUGAGAAGCCCCUCCGCCGGAAAGGACCAGGGUUUCCUGGCAACUUCAAUAGGCCAGGAGUUAGCCGGUCCUAAGGCGGGGCCUAAUAGGCACCCGCCGAAAGGGAAACGGGUUAAUAUUCCCGUGCCGCGGGGGUAGGUUCUGCGGCAACGCAGGCCCCGUCCCCGACGCCUCGGGAUAGGGCGGGCGGGACUGCCGUCCCGCUUAACCGUCGAAGGCCGGGGAGUGCCGUAAUGGCGAGAACCGGCCGAAGGCGGGAAUAGCCGGGGGUUUCCCCGGUCCGCCCGACUCCUGGGGCCCGUGAAAAGGGGACGGGGAACGAGCCCCCGCGCCCGUACCGAGAACCGACGCAGGUGCUCCUGGGUGAGAAGCCCAAGGCGGCUCGGGUGACCCCGGGCCAGGGAACUCGGCAAAUUGGCCCCGUAACUUCGGGAGAAGGGGUGCCUGCGGUCUUGGGGUAUACCCCCGGGACCGCAGGUCGCAGUGGCAAGGGGGACCUGACUGUUUAACAAAAACAUAGGUCCCCGCGAGCCCGUAAGGGUGUGUACGGGGGCUGAAUCCUGGCCACUGGCGGUACGUGAACCCCGGGUACAACCGGGCGAAGCGCNGCUGAAGGCCGGGGGUAACUCUGACCCUCUUAAGGUAGCCAAAUGCCUUGCCGGGUAAGUUCCGGCGUGCAUGAAUGGAUCAACGAGGUCCCCACUGUCCCGGCCCGGGGCCCGGCGAACCCACCUCCAGGUGCACAGUCCUGGGACCCCCGACGGGGCGAGAAGUCCCUAUGGAGCUUCACAGCAGCCUGUCGUUGCGGGGGGGCGGGGGGUGCAGAGCGUAGGUGGGAGCGAUGAAACGGGGUCUCCGGGCCCCGUGGAUGCGACCCUGGAACACCACCCACUCUCCGCCCCUCCGCUUACCCGCCGCAAGGCGGGGACAGCGGCAGGCGGGCUGUUCGGCUGGGGCGGCACACCCCUGAAAAGAUAUCGGGGGUGCCCAAAGCUCGGCUCAGGCGGGUCAGAAAUCCGCCGUAGAGUGUAAGGGCAAAAGCCGGGCUGACUGGGCCCUUGAACGCAAGGGGCCCAGGCGGGAAACCGGGGCCUAGAGAACGCUCGUGCCCCCACCAGUGGGGGCCGGGCAUGACAGAAAAGUUACCCUAGGAAUAACCGGCUCGUCGCGGGUGAGAGUCCCCAUCGACCCCGCGGUUUGGUACCCAGACGUCGUCUCUUCCCAUCCUGGCGGUGCAGCAGCCGCCAAGGGUGGGGCUGCCCGCCCAUUAAAGGGGAACGUGAGAUGGGUUCAGACCGUCGCGAGACAGGUCGGUCUCUACCUGUCGGGGGCGCUGGCCGCCUGAGGGGAAGGUGCCCUCAGUACGAGAGGAACGGGGCGCCGCGGCCUCUAGUGUACCGGUUGUCCGGCAGGGCACUGCCGGGCAGCCACGCCGUGGGGGAUAACCGCUGAAAGCAUCUAAGCGGGAAGCCCUCCCCGAGACGAGGCGGCCGUUGCCCUGGGGGCAACCCCGGGGCACGAGGGCUCCCGUAGAAGACGGGGUUGAUGGGGGGGCGGUGUAACCCCCGAGGGUUUCCCGAGGGGAGAGCCGGCCCCUCCCAAUCGCCCGAGCGUGC

Entity 2 polymer

描述 description	5S rRNA
分子量 formula_weight	41804.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	2

GCCCCUAACCCGGCCAUAGGCGCCGGUGCUACACCCGGUCUCAUCAGAACCCGGAAGUUAAGGCCGGCGCCGCGCUCGGGAGUACUGGGCUCCGCGAGGGCCCGGGAAACCGGCGUGCUGGGAGGGGGC

Entity 3 polymer

描述 description	Putative signal-transduction protein with CBS domains
分子量 formula_weight	72127.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3

MLTREELEKLAEKRVPQFVRDVVEHPPFRVTVNTTLETLAAYLRKFPVDVVPVFKSVFSDEVAGVVYPHTALLLKSKKLDAKVGEFLNQPLVVKESWRIENVAEMLISESKWGAVVVDEEGKFVGVVSLRGLLSALLLREPKAKSVAAVYTSIDEKKPRVGFVKAIEKVSKIFHKLVGGEVDGYVVLNREGGAAGILTVWNFLKSRRWFRGSGEPRAIFGTRVTRGESKPRGVARVWRIMSRGVAVANPDTPITDVARYMATFGIYVVPVVDRNGKVIGAVTAWDVLHAYLYGPKEGREDVEVKTAVEVPVSKPSMEAVIRLKPVKHVTGLRARDVMLSDVPMVNVRDPLSSIRKAFLRSKSGILAVVDDSGKVVGFITRRDFMSYIAEKSLGYWRRQKGKLLVLREEALPGEQAKLAVEEGTAGDLAKSEYPTVSESATLEEVAYKMLAAGTDYVVVIDSAGQPIGVVTKDELVKAYKERGRSVKVGELMTPADVAAVNPFSSLMSTTRKINAFELDGVVVVEGGNIMGVVTVDDLALRPVEETLRGERVVYFTKSGVLRKATTGLSRLRYSKVGTLTALDVSRPVDHVANVNADVKEVLDKLVEQGVVPVVDEQGRLVGVLNKMDIVKDLARVYVTYAMPEKLAEVKQVEERA

Entity 4 polymer

描述 description	Large ribosomal subunit protein uL2
分子量 formula_weight	26240.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AA

MGKRILVQRRGRGGSQFRSPSWKRDGPVRYPPNISGRGIVVEILHEPGLNAPVAKIRMENGVEFFNYAAEGLYVGQVIQVGPDAPPAVGNVLPLGKIPEGTMVFNVEKRFGDGGKFARSGGTYALVIGQRPEENKTIVRLPSGRVIEVDARGRATIGIVAGGGRVEKPFVKAGKKYHRARAKSWKYPTVRGKAMSPYAHPHGGGSHQKGGTPVPKTAPPGQKVGFIGSRCTGRGCVRARAQQKQ

Entity 5 polymer

描述 description	Large ribosomal subunit protein uL3
分子量 formula_weight	37240.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AB

MGLKINRPRRGSMGVYPRKRAADIVPRVRTWPEVNLGKPTLLGFAAYKAGMLHAVVVDDRPTSPLYGKEVVKAVTVLDAPPLYVAAVRLYTLDPTNGYKVAVGEAWVSEPPADLRRVLTLPEKFDTEKQLKALEEYRDVAVDVRVLVATQPRLSGIGKKTPEVLEIPVGGVPSIDERINFAISLLGKTVSPKDVFTPGQLVDVIAVTKGKGYQGVVKRFGVTILPRWHKHRKGHRRTGTIGPQAPALMFTQPRPGQMGFHQRTEYNKRILKIGDNGAEITPKSGFPHYGVIKGPYILLQGSVPGARKRLVVLRYPVRPPKKAPPAAEPQVVWVSSQSI

Entity 6 polymer

描述 description	Large ribosomal subunit protein uL4
分子量 formula_weight	31602.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AC

MGAVVKELLKFKLLDLSPYIKPAEERPPEALKVYDVNGQYMADIETPIHFYEPVRPDLIRRAYLSALSARFQPKGVYEGAGKEHSCESFGVGLGIARIPRYKGHLWPRGCFAPNTRGGRRAHPPRPEKKLHEEINWKEKNLAIRSAIAATAYKSWVAARGHMVEKVPSLPLVVSGDAEKIAKAKEAKKLFEVLGLWPDVERAAEGVKIRAGKGKMRGRRYKEPKSVLVVVSELDVPLIGAVRNFPGVDVVPVSHLNMLVLAPGGVPGRLTLWTATAVERLKGLFL

Entity 7 polymer

描述 description	Large ribosomal subunit protein uL5
分子量 formula_weight	20156.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AD

MKWKELVLVKDHPMKRVYIEKVVVNIGVGTGGERLEKAANLLRELTGAEPSLRRAKRSIKDFGIRKGEPIGVAVTLRRDKAVEFLMRALQAVGNRIKRSSFDERGNVCFGIKEHIMLPGVKYDPAVGIWGMDVCVRLAKPGLRVQLRRRRRSKVGKGQLVTREEAVEFFQKVLGVQVD

Entity 8 polymer

描述 description	Large ribosomal subunit protein uL6
分子量 formula_weight	21663.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AE

MHVVYAVEEVPIPDGVKVAIEKTGPFDYVVKVKGPLGELVKEFKNTPVIMSLSDGKVVLEVLNAKKREYALLGTYKGILKNMFLGVTKGWRYKLKVIYTHFPMLVKVQGNQLTIENFLGRKSKIVLEIPKGVKVEVKGKEDIVVEGIDRELVSQFAAAIQAATELRGEEKPSPHGREGGLGVVDGIYVVGYEHVKA

Entity 9 polymer

描述 description	Large ribosomal subunit protein eL8
分子量 formula_weight	16013.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AF

MAVTIDPKTFYANPLPGKPFYVRFEVPSDVAEKALEILSIARQTGKIKKGTNETTKAVERGLAKLVLIAEDVDPPEVVAHLPLLCEEKKVPYVYVPSKEKLGKAAGINVAAAAAVVIEAGQAAGELEALVNKINEIRAKHGLNAIPVRR

Entity 10 polymer

描述 description	Large ribosomal subunit protein uL13
分子量 formula_weight	21737.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AG

MIVKKVLDLSQIPEKGEIVIDAEGHIMGRLASYVAKILLSKPELRVVVVNAEKLVVTGDRKMVVEWFMRKISEWRTHYNPEKAGPKIPRRPDRVFKRVVRGMLPKKVESGRDALKRLRVYMSIPLDFIQRRRLVLYEVPAAKLRVRPLMQFVTLEEVWRSIDPAAWEKWNKAKEVWAKKIKQATSG

Entity 11 polymer

描述 description	Large ribosomal subunit protein eL13
分子量 formula_weight	17939.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AH

MIPKPVVQVPRSIKSGGVTKWKEGRGFSLGELKEVGLNAEQARLLGLPVDTRRDSVWPQNVEALRKWLIEVLEGKATPPEPTLPKVVKVKRKRGRAFRGLTSAGRRSRGLISTRFRETHNYKFKKKAKERRLKKRHEATKGLGNVLRISRIINESKE

Entity 12 polymer

描述 description	Large ribosomal subunit protein uL14
分子量 formula_weight	15768.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AI

MAKRGGKRTVGVPYRFHVTPGIFMNSLVPVADNSGAKLVRVIGVVGHYSKSVHRRIPGAGVGDMVVVVVREGKPELRKQIFRAIVVRQRRPYRRPDGTWVAFEDNAVVIVTPEGDPKGSEIHGPVAMEATLRWPTIANLASIVV

Entity 13 polymer

描述 description	eL14
分子量 formula_weight	11470.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AJ,AK

MVKVIDVGRVVVKVLGREAGRKAVVVDIVDENYVVITGPKSLSGVRRRRVNINHIEPTDKKIEIKKGASDEEVLKALEAAGLVEYMKERVKPKLFEITPADVK

Entity 14 polymer

描述 description	Large ribosomal subunit protein uL15
分子量 formula_weight	17525.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AL

MVRRFKRATKYRRGSRTHGWGRVGQHRKSGGSGGKGMVGFHKHKWSLVMKYGESGTGWPFYGKHGFKQPETISVEWRPINVGRLAELVKELRERGEVREEEGRLVVDLLKLGYNKLLGGGHMDVPLVVYTPVASKAAVEKIAKAGGEVRIVSLVHR

Entity 15 polymer

描述 description	50S ribosomal protein L15e
分子量 formula_weight	22623.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AM

MPSAYQYMAMWYRKVGREVHERLMRSVLIKWRKEPAIVRVERPFRLERARKLGYKPKQGVVVARVRLIRGPFNRRRPDSGRRPKRMGVYGITAWKSWRQVAEERAARKFPGLEVLNSYWVADDGIYRYFEVILIDCNQPTIQRDPLYSGICGREVPRRRLARRLRERQRKLIEHLKKTLLPKLKGTSTA

Entity 16 polymer

描述 description	Large ribosomal subunit protein uL16
分子量 formula_weight	19893.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AN

MPARPARCYRRIKGPPYTREEYIHGAPMIQIPKFDMGTTSAAARAAFTMVAKLVAEERGQIRMQALEAARQISSKYLTKYVGDANYYLRLNVVPHHVLRENRMLAMAGADRLQEGMRLAFGSPAGRAARVEPGQVIFYVEFKPEHLAHVKEALRRAASKLPIPTRIVVEPKGDGKTAS

Entity 17 polymer

描述 description	Large ribosomal subunit protein uL18
分子量 formula_weight	23424.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AO

MARGARYKVPFRRRREGVTNYRKRRKLLLSRKPRLVVRKTNKHLIAQIVIARPHGDETVVGLDTRVLSKFGWKGDENNTSAAYLLGLIVGYKARAKGIKEAILDIGLHRPTPGARVFAVMKGVIDAGVEVPHGEEVVPSEERIRGEHVAEYAKRLKEEAPEAYKARFSRYLQRGLPPEELPSHFEEVKKKIVEYYEQKLAKAVAR

Entity 18 polymer

描述 description	Large ribosomal subunit protein eL18
分子量 formula_weight	13224.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AP

MPPNPTGPTNRQLRMLIRFLRKAATANSANIWRAIAELVERPRRSRVVVNVGKLNRVANDGDVVVIPGKLLGGGELKKRVVVAAVSVTPKAWKKVVEAGGEVLTIPELVRRNPKGSGVKIVI

Entity 19 polymer

描述 description	Large ribosomal subunit protein eL19
分子量 formula_weight	17414.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AQ

MVDVKRLAAEILGVGVSRVKISPEAVGRLEEVVTKDDVRALIDEGLIWAEPEKGNSRGRWRELHEKKKKGRRRGQGSRKGRRLDEEEAWVNKMRVMRRFLNTLKRQGKLDPATWRQLYRMVKGNYFRDLRHLKTYINEHKLTKEPVK

Entity 20 polymer

描述 description	Large ribosomal subunit protein eL20
分子量 formula_weight	8929.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AR

MPKIYRVVGDTSTGMKFVVEVVGERPYDALEKVYSVLGSRHKLKRTQIRIREVSVVEPSSAKSNEAKMLMVLEKVVRY

Entity 21 polymer

描述 description	Large ribosomal subunit protein eL21
分子量 formula_weight	11319.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AS

MVKRTHGYRYKSRKLLSKAPRERGMSGLSRLLYEYKPGDKVVIDIDPTFISTAPHRRYQGKVGVVIGTRGRAYVIETYIGDKKKIIITTPEHLRPHLGG

Entity 22 polymer

描述 description	Large ribosomal subunit protein uL22
分子量 formula_weight	21309.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AT

MPHFHYSLTDEQAAELVFKKYGVRITPEQIAKAYAPEQRMSWKKSVEVARFIKGMTLKQAKAWLEDVVKLKRPIPIKTFKKKQAHHATPWEGWPVAKWPVKVAKRYLQLLENLENNAKFKGLDVERLVIIHAAAHKGIKIPNIMPRAFGRATPFNEETVNVEIVAAELPKEVVPKRYKLNLVKR

Entity 23 polymer

描述 description	Large ribosomal subunit protein uL23
分子量 formula_weight	9307.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AU

MIKRFVITEKALRLAERENKITIIVERTATKKQIADEVQRLYNVKVEKVNTLITPRGEKKAYVKLAEGYSAMDLLTKLGVL

Entity 24 polymer

描述 description	Large ribosomal subunit protein uL24
分子量 formula_weight	14588.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AV

MPFTASAQPRKQRLSLYAAPLHLRRKLLNAKLSPELQKKLGVKRLPVRRGDTVLIMRGDFKGVTGKVVKVDLKRVRIFVEGATRTNSRGQTVYYPIHPSKVMIVDVDLSDKARQKLIERRKRGQHGSS

Entity 25 polymer

描述 description	Large ribosomal subunit protein eL24
分子量 formula_weight	7102.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AW

MKIHKCSFCGADIPPGYGIMYVRSDGTVQRFCSRKCFVSAVKYGRNPRKLAWVRKKKAKTSK

Entity 26 polymer

描述 description	Large ribosomal subunit protein uL29
分子量 formula_weight	9158.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AX

MSERKLKPKALREMSPEERRELLNQLRAELVKLQTQRSRGFVEKPGRIRQVRRMIARILTIERELAKAGARAEKGGAKA

Entity 27 polymer

描述 description	Large ribosomal subunit protein uL30
分子量 formula_weight	20287.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AY

MMAATEQKTVYPRYAVIRLRGIPTTPGDIAATLDLLRLRRKFTMVVVVGSPSVMGMIERVNDWVTWGEIDADTLAEVLRKRGRIVGDKPLTLEYLQKWGWNSFEEVALAYISGEIETLACKKDFKAREGQRPPCIPYLKPFFRLHPPRGGLNSVKLHFSVGGDLGYRGPLINDLIKRML

Entity 28 polymer

描述 description	Large ribosomal subunit protein eL30
分子量 formula_weight	10886.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AZ

MIDIGRELQVAINTGKVIIGLRETKKSLLTGAPKLVIIAANAPRWAREDIEYYAKLAGVPIFTFPGSSIELGAAAKRPHKIMALAVVDPGQSEILKLVEHA

Entity 29 polymer

描述 description	Large ribosomal subunit protein eL31
分子量 formula_weight	10668.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Aa

MSEEKKVVLTREYVINLRRTREVSRTKRAKYAVGLIRRFVARHLKVEPKAVKLGQALNEALWARSIEKPPRRVHVVVEKLDDGTVRVELKQ

Entity 30 polymer

描述 description	Large ribosomal subunit protein eL32
分子量 formula_weight	18172.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ab

MVVVPRRELPQPLKRLLRLRLQMKRRKPEFVRIDQWRYKRIEDSGWRNQRTLDNKIRRKWKGWPKPVEVGYRKPAAVRGLHPSGFVEVLVHRPEDLAGLDPKVHAVRIGRTVGLRKRLEIVKKARELGFYVLNPGKEVVELLKKELNTAQPQQ

Entity 31 polymer

描述 description	Large ribosomal subunit protein eL34
分子量 formula_weight	9373.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ac

MPRPAYRSRSLRRVKVKTPGGRTVVHYEKRAKGVPKCAVTGQPLGGMNSKVYRFGISTRAPSRPYGGVVSHKVLARAIRYAVRR

Entity 32 polymer

描述 description	Large ribosomal subunit protein eL37
分子量 formula_weight	6174.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ad

MKGTPSMGKHNKGKTHIRCPRCGRHSYNVTKKYCASCGWGRSKRWRRYNWAK

Entity 33 polymer

描述 description	LSU ribosomal protein L38E
分子量 formula_weight	7954.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ae

MPKEIFLVELWKKYGEKAQVVRVKRYEDFVKLKARLSGYLYTIRVPPDKAEQLISELKAKNVKVEEV

Entity 34 polymer

描述 description	Large ribosomal subunit protein eL39
分子量 formula_weight	6030.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Af

MARNKPLGKKLRLAAALNSNRNPPLWVIAKTKRRVTRSPARRHWRRQKLKA

Entity 35 polymer

描述 description	Large ribosomal subunit protein eL40
分子量 formula_weight	6356.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ag

MPITLDPEKLAIVLQHRFNYKICRNCGARNPPDAEKCRRCRSRNLRPKKFKKK

Entity 36 polymer

描述 description	eL42
分子量 formula_weight	10603.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ah

MKFPKSVNMYCPRCNTYTQHSVSNYHGGQRRSLAEGQRRYERKLKGYGSTPKPKQKRFAKVNKKVTLVFTCTKCGYKIVKSLGRMKKVELV

Entity 37 polymer

描述 description	Large ribosomal subunit protein eL43
分子量 formula_weight	11387.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ai

MPFSHTKIVGPAGRYGARYGMGIRRKVTTIEVKQRGKHRCPSCRSLVRLERLAFGIWHCPKCGHTFAGGAYVPQTILGKTIAPEELKGVEAEKAKWKEAAKR

Entity 38 polymer

描述 description	DJ-1/PfpI domain-containing protein
分子量 formula_weight	20956.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Aj

MPKALLYVIDTAKRDEYPTLKNFLVKGGYEVDVAVGSRDVNVNYTVDLMTLPSDKLPTLFEEYDAFALAGGFKIYYHVLQKKPPLKIWDLNIDKERLAQLVVEFNRRRKLLIAPMAVPAYVAQLGLLSGRHATVYPVTELIKILRDNKVNFVNKQVVRSENIITVKDITTTSEKEFLAALREKG

Entity 39 polymer

描述 description	PaREP1 domain containing protein
分子量 formula_weight	10938.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ak

MPLCSKWESLITWAEKGGNSYKALEFREKLVECIVYTATEKVKKKKLDEAEELLKYGKEVAKKFNIEELNFHIALLEKEISKIRQRRAQAQTK

Entity 40 non-polymer

描述 description	SPERMINE
分子量 formula_weight	202.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	46

Entity 41 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	173

Entity 42 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	5

Entity 43 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	7994

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	1.95 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	183467

fit	/app/data/saxs_computation/e6/9e6q/9e6q.fit
pddf	/app/data/pr_computation/e6/9e6q/9e6q.pr.out
profile	/app/data/saxs_computation/e6/9e6q/9e6q.dat

Cryo-EM structure of the Pyrobaculum calidifontis 50S ribosomal subunit in complex with Dri

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (43)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 polymer

聚合体 Polymer

Entity 34 polymer

聚合体 Polymer

Entity 35 polymer