Co-crystal structure of yeast Forkhead transcription factor Fkh1 bound to DNA

条目编号 entry_id	9efw
沉积日期 deposition_date	2024-11-20
结构标题 title	Co-crystal structure of yeast Forkhead transcription factor Fkh1 bound to DNA
关键词 keywords	DNA binding, Transcription factor, DNA shape readout, Forkhead proteins, DNA BINDING PROTEIN, DNA BINDING PROTEIN-DNA complex; DNA BINDING PROTEIN/DNA
实验方法 method	X-RAY DIFFRACTION

回转半径 Rg (Guinier) rg_guinier	25.46 Å
回转半径 Rg (电子) rg_electron	24.79 Å
零角强度 I(0) i0	68298700.00
分子量 molecular_weight	50129.0 kDa
排除体积 excluded_volume	56495 ų
包络体积 envelope_volume	78544 ų
水化壳体积 shell_volume	26934 ų
包络直径 envelope_diameter	89.4 Å
壳层 Rg shell_rg	31.50 Å
包络 Rg envelope_rg	24.55 Å
形状 Rg shape_rg	24.78 Å
总 Rg total_rg	25.45 Å
总原子数 total_atoms	3436
残基数 n_residues	312
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å−1
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/ų
壳层衬度 contrast_shell	0.0300 e/ų
CRYSOL 版本 crysol_version	4.1.3

最大尺寸 Dmax dmax	85.3 Å
Rg (实空间) rg_real	25.37 Å
Rg 误差 (实空间) rg_real_error	0.61 Å
I(0) (实空间) i0_real	6.8300e+07
I(0) 误差 (实空间) i0_real_error	8.9970e+05
Rg (倒空间) rg_reciprocal	25.40 Å
I(0) (倒空间) i0_reciprocal	68300000.0000
解质量估计 total_estimate	0.8797
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	33.0 Å
偏度 Skewness skewness	0.199
峰度 Kurtosis kurtosis	-0.368
角度范围 angular_range	— – 0.3100 Å−1
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	3567000.0000
实空间数据点数 n_real_points	63
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.825; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.957

晶胞参数 Unit Cell

晶胞边长 a length_a	48.161 Å
晶胞边长 b length_b	50.245 Å
晶胞边长 c length_c	59.041 Å
晶胞夹角 α angle_alpha	110.78 °
晶胞夹角 β angle_beta	100.37 °
晶胞夹角 γ angle_gamma	82.63 °
晶胞分子数 Z z_pdb	2

对称性 Symmetry

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	triclinic

精修参数 Refinement

分辨率 resolution	2.200 Å
最低分辨率 resolution_low	29.510 Å
R 因子 r_factor	0.1914
R_work r_work	0.1889
R_free r_free	0.2374

衍射实验 Diffraction

实验温度 temperature

100.0 K

Files (7)

• pr_distribution /app/data/pr_computation/ef/9efw/pr_distribution.json
• pr_output /app/data/pr_computation/ef/9efw/9efw.pr.out
• pr_plot /app/data/visualization/pr_computation/ef/9efw/9efw_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/ef/9efw/9efw.dat
• saxs_fit /app/data/saxs_computation/ef/9efw/9efw.fit
• saxs_log /app/data/saxs_computation/ef/9efw/9efw.log
• saxs_plot /app/data/visualization/saxs_computation/ef/9efw/9efw_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/ef/9efw/9efw.fit
pddf	/app/data/pr_computation/ef/9efw/9efw.pr.out
profile	/app/data/saxs_computation/ef/9efw/9efw.dat