SAXSdb — 9eoj

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9eoj
沉积日期 deposition_date	2024-03-15
结构标题 title	Vertebrate microtubule-capping gamma-tubulin ring complex
关键词 keywords	cytoskeleton, microtubule, microtubule nucleation, complex, template, cap, gamma-tubulin, gamma-tubulin ring complex, CELL CYCLE; CELL CYCLE
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	109.50 Å
零角强度 I(0) i0	37218900000.00
分子量 molecular_weight	1672400.0 kDa
排除体积 excluded_volume	2100900 Å³
包络体积 envelope_volume	4271900 Å³
水化壳体积 shell_volume	327050 Å³
包络直径 envelope_diameter	319.0 Å
壳层 Rg shell_rg	113.40 Å
包络 Rg envelope_rg	99.05 Å
形状 Rg shape_rg	109.50 Å
总 Rg total_rg	109.50 Å
总原子数 total_atoms	117778
残基数 n_residues	14563
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	369.5 Å
Rg (实空间) rg_real	113.80 Å
Rg 误差 (实空间) rg_real_error	2.42 Å
I(0) (实空间) i0_real	3.7220e+10
I(0) 误差 (实空间) i0_real_error	8.5780e+08
Rg (倒空间) rg_reciprocal	113.50 Å
I(0) (倒空间) i0_reciprocal	37550000000.0000
解质量估计 total_estimate	0.8656
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	158.4 Å
偏度 Skewness skewness	0.140
峰度 Kurtosis kurtosis	-0.334
角度范围 angular_range	— – 0.0700 Å⁻¹
当前正则化参数 α current_alpha	0.9838
最高正则化参数 α highest_alpha	731600000.0000
实空间数据点数 n_real_points	15
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.869; Stabil: 0.905; Sysdev: 1.000; Positv: 1.000; Valcen: 0.978; Smooth: 0.004

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (7)

Entity 1 polymer

描述 description	Mitotic-spindle organizing protein 1
分子量 formula_weight	7749.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B,D
原子数 atom_count	117778

MANASGNMSAVRETMDVLLEISRLLNTGLDMETLSICVRLCEQGINPEALSSVIKELRRASDTLKASESTAS

Entity 2 polymer

描述 description	Gamma-tubulin complex component 3 homolog
分子量 formula_weight	103789.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	5

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q,c,d,e,f

MAVPDQKSPNVLLQNLCCRILGKGEADVAQQFQYAVRVIGSNFAPTVERDEFLVTEKIKKEFVRQRREADGALFSELHRKLQSQGVLKNRWSILYLLLSLSEDPRKQPNKTSSFAALFAQALPRDAHSTPYYYARPQSLPLSYQDRNVQCAQNAASIGSSGISSIGMYALNGPTPQSIIQGQSNQTPNMGDALRQQLGSRLAWTLAAGQQPSQQSTTTKGLPNTVSRNVPRTRREGDSSGSVEITETSLVRDLLYVFQGIDGKFVKMCNSENCYKVDGKVAVSKSLKDITSKLSELGWLHNKIKKYTDQRSLDRAFGLVGQSFCAALHQELKEYYRLLSVLHSQLQVEDDQGVNLGVESSLTLRRLLVWTFDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSLVQHILGLVAYPILNFLYRWIYDGELEDTYHEFFVASDPVVKTDRLWHDKYSLRKSMIPSFMTMDQSRKVLLIGKSINFLHQVCHDQTPASKAMAVGKSAESPKDAAELFTDLENAFQTKIDAAYFDTSKYLLDVLNKNYNLLEHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQHNLTGILETAVRATNAQFDNPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRECMSHYLRVFNFLWRAKRMEYILTDIWKGHMCNAKLLKGMPELSGVLHQCHILASEMVHFIHQMQYYITFEVLECSWDELWNKVLKAQDLDHIIAAHDVFLDTIISRCLLDSESRALLNQLRAVFDQIIEFQNAQDALYRAALEELQQRLQFEERKKERESEGEWGVTAAEEDVENKRIQEFQESIPKMRSQLRILTHFYQGIVQQFLVLLTTSTDESLRFLSFRLDFNEHYKAREPRLRVSMGTRGRRSFHV

Entity 3 polymer

描述 description	Gamma-tubulin complex component
分子量 formula_weight	103468.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	5

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R,Y,Z,a,b

MSEFRIHHDVNELISLLHVFGLEGADVYIDLLQKNRTPYVTTSVSTHSAKVKIAEFSRTPDDFLKKYEELKSKNTRNLDPLVYLLSKLIEDKETLQYLQQNAKDKAELATSSVTSVSLPIAPNTSKISMQELEELRRQLETATVAVSCSHQPVEVLRKFLRDKLNKKHTGHPVPVFPSWVYERPALTGDFMSFSNPSTDVTVSIGTLPLPSQETCLVEDLLYILIGVDGRYISVQPLVGRQSRSFSVEQNLDSSVKELVNRILPVATNYSTVTRFVEENSSFEYGQVNHALGAAMRTLGKEYMILISQLEHLQRQGLLSLQKLWFYIQPTLRTMEVLASIATSLNKGECFGGATLSLLHDRTFGYTGDSQAQELCLYLTKAASAPYFDILERWIYRGIINDPYSEFMVEEHELQKEKIQEDYNDKYWDQRYTIVQQQIPSFLQKVADKILSTGKYLNVVRECGHDVTCPDAKEITYTLKEQAYVERIEKAYNYASKVLLDFLMEEEELVAHLRSIKHYFLMDQGDFFVHFMDLTEEELKKPVDDIIPTRLEALLELALRMSTANTDPFKDDLKIELMPHDLITQLLRVLAIETHQEKALINSDPTELALSGLESFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERLLCNVWISNKTAKQFSLHSAKWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLSHHTSFLDNCLKDCMLTNPELLKIFSKLMSVCVMFTNCLQRFTQSMQVQTEMEHLTLEHGTMMGPPTQCERTEEALKKKLTSKYLEEHIDKFPSSFGFESTINNFDSNFSAHLMDLLDKLSMYSTSDCEHSMINIIYRLDFNGFYTERLKQLSSERNQKSAPLLGPAQHAVSTK

Entity 4 polymer

描述 description	Gamma-tubulin complex component 6
分子量 formula_weight	193069.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S

MDSITKLFGDLCESHMVGFPWRTALNSRKHSKNRTKQTLKKLAYDTLFVHLFQDEARKLQPNCTRLPVKNKIIMLSFNLRICGMSSEADRLEELVEYLEQSNGIQISDLHAVLELLVELSGTGPPQLLPPKRDYFKNNKYVGRNVKYQGYDYYDVQVFEADLGTTVAYQELEISTTIQRTLQIMEAAPGTGLPALSFFSQNDLSTDKFEKETRGSLFGALVHSRTNDMDIKLDMPPVPENADLSGLAIKVPQSIDQSEDEGFQSASNMTPDSQSEPSMTPDIDVWEAVLTYGPSKRRCWERIGCPPGKREEPYVTEAGREAFDKLYKLHEGGLQILSATTLQPQLVLLEETDLVKAVLNVLIGVVSSTFSYNQALQSFAVKQGVYISGTSPDNVSSLLTQVAEYGTYYTRLSHFSLLTVLDSSHSNGLVFQAFTSGLRKYLQYYRACVLSTPASLTLLTISFLFRKLGRQLRYLAELCCIGTLVTSATRGISTAFPTGVKLLSYLYKEALENSSNENYPVLLSLLKTSCEPYTRFIYDWVYSGVFRDVCGEFMIQVNEDYLGFRDKRYWTHGYVLISKEVEDCVPVFLKHVANEIYICGKTINLLKLCCPKHYICWSDIPVPRISVTFSLEELKEMEKDCAVYVARMERIARHSCISKEQKALQTEIARQELIIQARETTEKVFETFKDRKLAEKLSLDTKKRELFQKLKDQYEKEQERRLTTKQEEADDDFSYAREIRDREKRLKALEEELELKTRQELIEHYSRLSEEATRKEQRALWKLQRHKLETIRLKFFLEEQKRMQDLVANFPVDICEENLGVLPDGEISHQTDNTNDAGLGNIENEKSVPEQHALHNNNDEVYTAQNCISKSESLCVDVTLPTENVHSQTSNASVLGVPSFDSNLCTPDVDIIDFLPTLPSENQEVAVVQSLVDDALISIGSDLNTDTKDKESLCALKSDLQESSTGSEYDFKTILKPIACTQVSQGHIKIGEYSSNVQPARPRWSTHGHSSDSNIKIGNYVSDINVHQPKHSQHGHSSDSNINISDHMSDVEPRLPRLNLHGHISTGHIKVGEYASDVEPSTPRHSVHGHASQGNIKIGENVSDVKLSRPRWNIHGHVSDANIKIGENTSEIAPLRPRWNIHGHASQSHIKIGELVSDIEPSQPRRTPFGHPSQSSIPIGDQPVEKYAQKSESEVHSSNSTIQHLLYSNIPDKNKDTGGTLTDSPVPVPDQGNSNDDTEKRSSTLEQRVQAADSVCDGEASPNTAQSLPCMSDTLDFGTNGEENVGNDDHTWEKQQEYLKGLAEKYCLEKYQDSYELMSHPPVLHLYSNVMPNRFSFPTDSDIKSATDETTVQLIELLSLPVLMKYSVTAPMVSHVYLVNKAIVDYYFVELKMERHFEAMRHFLLMEDGEFAQSLSDMLFEKLGSGQTPSELLNPLVLNSILNKALQYSLHGDSSLASNLTFALKYLPEVFTPTAPDALSCLELKYKVDWPLNIVITDTCMNKYSRIFSFLLQLKHMVWTLRDVWFHLKRTALVNQASNSVQYRQLQLYRHEMQHFVKVIQGYIANQILHVTWCEFRNKLSAVSNLEEIYKTHADYLNKALFRGLLTEKAAPLMNIIHSIFSLILKFRLQLISQSWICDTGKQMAVHPNFGLMQQSYNTFKYYSDFLFEVVSKLVNRGYQPHLEDFLLRINFNSYYKQS

Entity 5 polymer

描述 description	Tubulin gamma-1 chain
分子量 formula_weight	51226.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	14

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T,h,i,k,l,m,n,o,p,q,r,s,t,w

MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYANLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSHVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTNISSLFERTCRQYDKLRKREAFLEQFRKEDIFKDNFDELDNSREIVQQLIDEYHAATRPDYISWGTQDK

Entity 6 polymer

描述 description	Gamma-tubulin complex component
分子量 formula_weight	117577.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	U

MAHWTRFERDQEGDIKKLVSLMSGIQDDQDGNFQQALQFAWSNFRFHRYLDVSSHTVLRTLEGIFEKLVVHSDLEKAESWKRLTEEFLLLPLPNTEGTKTDSHFAVLSLLLCLSDSPSNHDYTEKPRKKENDEQEPFDWGKYLREGEDIEFSPDADTPEWSEASEEEDAQEPPSREDSGIQVDRTPLEDPEKKGAPPLVSWKVGEPDARSWLEQHIVHQYWTSRAPRFSHSSHLHSNLSAIWDQHLYTTDPLYTPDDKTIVTETQVIRETLWLLSGVKKLLIFQLNDGKVNVRNDIIVTHMTQNCLRSVLEQIAAYGQVVFRLQKFIDEITGHGSEVPLPGTLPTAKKTTEAPFRTYQAFMWALYKYFISFKEELTEIEKCIINKDETVTLAIVLDKLAPRLAQLKVLHRVFSTGIAEVPPDTRNVVRASHLLNTLYKAILDYDNVGEASEQTVSLLFCLWVETVRPYLEIVDEWIVHGNLFDPAKEFIIQRNKDVPFNHRDFWYATYTLYSVSEKTENEDKMSDNASASSGSDQAPAGRQHTMVSFLKPVLKQIIMAGKSMQLLKNLKCRTALQQDSSRDSDRKSLYTLFLESVQSRLQHGNDSVPDIITEQQVNKLSLIKMQSIVAKHLELDEVHDPLLAINFVRLYLEQSDFLETFTCNEVCVDRSSESVTCQSFELTLRSCLYPHIGKQYLECCGNLMYTLKKDYRLVEYLQAMRNFFLLEAGDTMYDFYTPIFDKIREKEPWLNLSYLNVQIQEAVGQRYPDDSTRLSVSFESVDLAKKKLPVHTLDGLILSYKVPWPVDIVISSECQKIYNQVFLLLLLIKWAKYSLDVLQFNELGNASENESTKEGATVEPFPLPPLTSPSEPKGQQIHRMFLLRVKLMHFVNSLHNYLMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSSVKEAIMKVLNVVLMFADRWHAGLGAWKKESIVKMESDFTNCHKFLVKVLNKAVCRGSFPHLESLALSLMAGMEQS

Entity 7 polymer

描述 description	Gamma-tubulin complex component
分子量 formula_weight	76123.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	V,W

MIHELLLALSGYPGSIFTWNKRTGLQVSQDIPFLHPGETSVLNRLCKLGTDYIRFTEFIEQYTGHVQQQDHHPSQQGQVGLHGIYLRAFCRGLDSILQPYRQALLDLEQEFLADPHLSISHINYSLDQFHLLFPSIMVVVEQIKSQKIHGCQILETVYKHSCGGLPPVRSALEKTLAVCHGVMYKQLSAWMLHGLLLDQYEEFFVRQGSSSGNLAAAFEEEEDDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRAEMLPSYIPVRVAEKILFVGESVQMFENQNVNMSRTGSILKNQEDTFAAELHRLKQQPLFSLVDFESVLDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLLGRGELFQAFIDVAQNMLKTPPTAVTEHDVNVAFQLSAHKVLLDDDNLLPLLNLTIDYHGKEHKDTSQPREGPFRDMSPREAPTSGWAALGLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQSELQHCWALQMQRKHLESNKTDAIKWRLQNHMAFLVDNLQYYLQVDVLESQFSQLLQQINSTRDFESIRLAHDHFLSNLLAQSFILLKPVFHCLNEILELCHSFCSLVSQNLGPLDERGAGQLDILVKGFSCQSSLLFRILSSVRNHQINPDLAQLLLRLDYNKYYTQAGGTLGSFGL

▶

7. 引用文献 (1)

▶

分辨率 resolution	17.00 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	8497

fit	/app/data/saxs_computation/eo/9eoj/9eoj.fit
pddf	/app/data/pr_computation/eo/9eoj/9eoj.pr.out
profile	/app/data/saxs_computation/eo/9eoj/9eoj.dat

Vertebrate microtubule-capping gamma-tubulin ring complex

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (7)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)