9epc

Cryo-EM structure of the Plastid-encoded RNA polymerase from Sinapis alba

Method: ELECTRON MICROSCOPY D_max: 272.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9epc
沉积日期 deposition_date	2024-03-18
结构标题 title	Cryo-EM structure of the Plastid-encoded RNA polymerase from Sinapis alba
关键词 keywords	RNA polymerase Chloroplast Sinapis alba Cryo-EM, TRANSCRIPTION; TRANSCRIPTION
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	75.58 Å
回转半径 Rg (电子) rg_electron	75.33 Å
零角强度 I(0) i0	11799800000.00
分子量 molecular_weight	926810.0 kDa
排除体积 excluded_volume	1161000 Å³
包络体积 envelope_volume	1799300 Å³
水化壳体积 shell_volume	196260 Å³
包络直径 envelope_diameter	266.3 Å
壳层 Rg shell_rg	75.72 Å
包络 Rg envelope_rg	74.42 Å
形状 Rg shape_rg	75.35 Å
总 Rg total_rg	75.24 Å
总原子数 total_atoms	65291
残基数 n_residues	8155
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	272.2 Å
Rg (实空间) rg_real	79.44 Å
Rg 误差 (实空间) rg_real_error	1.80 Å
I(0) (实空间) i0_real	1.1860e+10
I(0) 误差 (实空间) i0_real_error	2.6770e+08
Rg (倒空间) rg_reciprocal	75.57 Å
I(0) (倒空间) i0_reciprocal	11800000000.0000
解质量估计 total_estimate	0.8927
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	90.7 Å
偏度 Skewness skewness	0.526
峰度 Kurtosis kurtosis	0.060
角度范围 angular_range	— – 0.1050 Å⁻¹
当前正则化参数 α current_alpha	1.0480
最高正则化参数 α highest_alpha	748900000.0000
实空间数据点数 n_real_points	22
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.827; Stabil: 0.860; Sysdev: 1.000; Positv: 1.000; Valcen: 0.981; Smooth: 0.596

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (25)

Entity 1 polymer

描述 description	DNA-directed RNA polymerase subunit alpha
分子量 formula_weight	37945.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	65302

MVREKVKVSTRTLQWKCVESRRDSKRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRAKFENIPHDYSNIVGIQESVHEILMNLNEIVLKSNLYGTRNALICVQGPGYITARDIILPPSVEIVDNTQHIATLTEPINLCIGLKIERNRGYSLKMSNNFEDRSYPIDAVFMPVQNANHSIHSYGNGNEKQEILFIEIWTNGSLTPKEALHEASRNLINLFIPFLHVEEETFYLENNQHQVTLPLFPFHNKLVNLRQKKKELAFQYIFIDQLELPPRIYNCLKKSNIHTLLDLLNNSQEDLIKMEHFHIEDVKKLLDILEKK

Entity 2 polymer

描述 description	RpoB, subunit beta
分子量 formula_weight	121223.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MLGDGKEGTSTIPGFNQIQFEGFYRFIDQGLIEELSKFPKIEDIDHEIEFQLFVETYQLVEPLIKERDAVYESLTYSSELYVSAGLIWKTNRNMQEQRIFIGNIPLMNSLGTFIVNGIYRVVINQILQSPGIYYQSELDHNGISVYTGTIISDWGGRLELEIDKKARIWARVSRKQKISILVLSSAMGSNLREILENVCYPEIFLSFLTDKEKKKIGSKENAILEFYQQFSCVGGDPIFSESLCKELQKKFFHQRCELGRIGRRNINWRLNLNIPQNNIFLLPRDILAAADHLIGMKFGMGTLDDMNHLKNKRIRSVADLLQDQLGLALARLENVVKGTIGGAIRHKLIPTPQNLVTSTPLTTTYESFFGLHPLSQVLDRTNPLTQIVHGRKLSYLGPGGLTGRTANFRIRDIHPSHYGRICPIDTSEGINVGLIGSLSIHARIGDWGSLESPFYELVEKSKKAQIRMLFLSPSQDEYYMIAAGNSLALNRGIQEEQVVPARYRQEFLTIAWEEVHLRSIFPFQYFSIGASLIPFIEHNDANRALMSSNMQRQAVPLSRSEKCIVGTGLERQVALDSGVPAIAEHEGKILYTDTEKIILSGNENTLSIPLIMYQRSNKNTCMHQKPQVRRGKCIKKGQILADGAATVGGELALGKNILVAYMPWEGYNFEDAVLISECLVYGDIYTSFHIRKYEIQTHVTTQGPERITKEIPHLEGRLLRNLDKNGIVMLGSWVETGDILVGKLTPQVAKESSYAPEDRLLRAILGIQVSTSKETCLKLPIGGRGRVIDVRWVQKKGGSSYNPEIIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGRPVDMVFNPLGVPSRMNVGQIFECSLGLAGSLLDRHYRIAPFDERYEQEASRKLVFSELYEASKQTANPWVFEPEYPGKSRIFDGRTGDPFEQPVIIGKPYILKLIHQVDDKIHGRSSGHYALVTQQPLRGRSKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDAPESFRLLVRELRSLALELNHFLVSEKNFQINRKEV

Entity 3 polymer

描述 description	;DNA-directed RNA polymerase subunit beta' ;
分子量 formula_weight	78761.9 kDa
EC 编号 ec_number	2.7.7.6
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MIDRYKHQQLRIGLVSPQQISAWATKKIPNGEIVGEVTKPYTFHYKTNKPEKDGLFCERIFGPIKSGICACGNYRVIGDEKEDPKFCEQCGVEFVDSRIRRYQMGYIKLTCPVTHVWYLKRLPSYIANLLDKPLKELEGLVYCDFSFARPITKKPTFLRLRGSFEYEIQSWKYSIPLFFTTQGFEIFRNREISTGAGAIREQLADLDLRIIIENSLVEWKQLGEEGPTGNEWEDRKIVRRKDFLVRRMELAKHFIRTNIEPEWMVLCLLPVLPPELRPIIQIEGGKLMSSDINELYRRVIYRNNTLTDLLTTSRSTPGELVMCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIVVGPSLSLHRCGLPREIAIELFQTFVIRGLIRQHLASNIGVAKSQIREKKPIVWEILQEVMQGHPVLLNRAPTLHRLGIQSFQPILVEGRTICLHPLVCKGFNADFDGDQMAVHVPLSLEAQAEARLLMFSHMNLLSPAIGDPISVPTQDMLIGLYVLTSGTRRGICANRYNPCNRKNYQNERIYETNYKYMKEPFFCNSYDAIGAYRQKRINLDSPLWLRWQLDQRVIASKEVPIEVHYESFGNYHEIYAHYLIVRSVKKETLYIYIRTTVGHISFYREIEEAIQGFSQACSYDT

Entity 4 polymer

描述 description	;DNA-directed RNA polymerase subunit beta'' ;
分子量 formula_weight	156388.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MAERANLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQVKTLGFQQATATSISLGIDDLLTIPSKGWLVQDAEQQSLILEKHHHYGNVHAVEKLRQSIEIWYATSEYLRQEMNPNFRMTDPFNPVHMMSFSGARGNASQVHQLVGMRGLMSDPQGQMIDLPIQSNLREGLSLTEYIISCYGARKGVVDTAVRTSDAGYLTRRLVEVVQHIVVRRTDCGTIRGISVSPRNKSRMMSERIFIQTLIGRVLADDIYIGSRCVAFRNQDLGIGLVNRFITFGTQSISIRTPFTCRSTSWICRLCYGRSPTHGDLVELGEAVGIIAGQSIGEPGTQLTLRTFHTGGVFTGGTAEHVRAPYNGKIKFNEDLVHPTRTRHGHPAFLCYIDLSVIIESEDIIHSVTIPPKSFLLVQNDQYVESEQVIAEIREGTYTFHFKERVRKYIYSDSEGEMHWSTDVSHAPEFTYSNVHLLPKTSHLWILSGGSCGSSLILFSIHKDQDQMNIPFLSVERKSISSLSVNNDQVSQKFFSSDFSDKKKSGIPNYSELNGIVGTSHYNFIYSAIFHENSDLLAKRRRNRFLIPFQSIQEQEQEKEFIPHSGISVEIPINGIFRRNSIFAFFDDPRYRRKSSGILKYGTLKADSIIQKEDMIEYRGVQKFKTKYEMKVDRFFFIPEEVHILPESSAIMVENYSIIGVDTRITLNIRSQVGGLIRVERKKKRIELKIFSGDIHFPDKTDKISRHSGILIPPGRGKTNSKESKNLKNWIYVQRITPTKKKFFVLVRPVATYEIADSINLATLFPKDLFREKDNIQLRVFNYILYGNGKPTRGISDTSIQLVRTCLVLNWDQDNKNSSLEEVRAFFVEVNTKGLIRDFIRIGLVKSHISYIRKRNNPPDSGLISADSMNPFYSISPKAGILHQSLRQNHGTIRMFLNRNKESQSLLILSSSNCFRIGPFNHVKYHNVINQSIKKKPLITIKNSSGPLGTAIQISNFYSFLPLLTYNQISVIKYLQLDNFKYIFQVIHSYLIDENGRIFNLDPYSNLVLNPFKLNWYFLHQNYNNNYCEETSTIISLGQFFCENVCIAKKEPYLKSGQVLIVQRDSVVIRSAKPYLATPGAKVHGHYREILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISLNLEKRIKGWNRCITRILGIPWGFLIGAELTIVQSRISLVNKIQKVYRSQGVQIHNRHIEIIVRQITSKVLVSEEGMSNVFLPGELIGLLRAERTGRALEEAICYRAVLLGITRASLNTQSFISEASFQETARVLAKAALRGRIDWLKGLKENVVLGGVIPAGTGFNKGLVHCSRQHTNILLEKKTKNLSLLEGDMRDILFYHREFCDSSI

Entity 5 polymer

描述 description	PAP1, pTAC3
分子量 formula_weight	103467.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F

MSLFFLNPALPSNSIHPIPRRAAGISSIRCSISAPEKKPRRRRKQQQKRENEDSSSFGSSEAVSALERSLRLTFMDELMERARNRDPSGVSEVIYDMIAAGLSPGPRSFHGLVVAHALNGDEQGAMHSLRKELGAGQRPLPETMIALVRLSGSKGNAQRGLELLAAMEKLNYDIRQAWLILVEELVRTNHLEEANKVFLKGARGGMRATDQLYDLMIEEDCKAGDHSNALDISYEMEAAGRFATTFHFNCLLSVQATCGIPEVAYATFENMEYGEDFMKPDTETYNWVIQAYTRADSYDRVQDVAELLGMMVEDYKRVQPNVKTHALLVECFTKYCVVKEAIRHFRALKNFEGGTKVLHNAGNFEDPLSLYLRALCREGRIVELIDALDAMRRDNQPIPPRAMIMSRKYRTLVSSWIEPLQEEAELGYEIDYLARYVEEGGLTGERKRWVPRRGKTPLDPDAAGFIYSNPIETSFKQRCLEDWKVHHRKLLRTLQSEGLPVLGDASESDYMRVMERLRNIIKGPAQNLLKPKAASKMVVSELKEELEAQGLPIDGTRNVLYQRVQKARRINKSRGRPLWVPPIEEEEEEVDEEVDELICRIKLHEGDTEFWKRRFLGEGLIETTAETKETDESSVATGEIENKTEVVAKEADDDEDDEEEEQEGDEDDDENEEEEEAVVVEPENRAEGEDLIKNKAADAKRHLQMIGVQLLKESDEANRTKKRGKRASRMTLEDDADEDWFPEEPFEAFKEMRERKVFDVSDMYTIADVWGWTWEKDFKNKTPRRWSQEWEVELAIVLMAKVIELGGVPTIGDCAVILRAAIRAPMPSSFLKILQTTHSLGYAFGSPLYDEIITLCLDLGELDAAIAIVADMETTGITVPDQTLDKVISARQSNEIPKSEHEEPPSSSESS

Entity 6 polymer

描述 description	PAP2, pTAC2
分子量 formula_weight	122664.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

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

Entity 7 polymer

描述 description	PAP3, pTAC10
分子量 formula_weight	79815.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H

MQICQATLTTFTFTNPSNPNFCKPKPLFPSFQPPRRVTLPPCRGFSSDEFPVDETFLEKFGPKDKDTEDEARRRNWIERGWAPWEEILTPEADFARKSLNEGEEVPLQSPEAIEAFKMLRPSYRKKKIKEMGITEDEWYAKQFEIRGDKPPPLDTSWAGPLVVRQIPPRDWPPKGWEVDRKELEFIREAHKLMAERVWLEDLDKDLKVGEDATVDKMCLERFKVFLKQYNEWVEANKDRLEEDSYKYDQDFYPGRRIRGKDYKEGMYELPFYYPGMICEGTVTTLHLYQGAFVDIGGVHEGWVPIKGNDWFWIRHFIRVGMHVIVEITAKRDPYRFRFPLELRFVHPNIDHMIFNKFDFPPIFHRDGDTNPDEIRRDCGRPPEPRKDPGSKPEEEGLLSDHPYVDKLWQLHVAEQMILDDYEANPEKYKGKKLSELSDDEGFDERKEIEHGEAYYKKTKLPKVILKTSVKELDLEAALIERKYHNKLMMEAKARGEGYKIEKLRRNIEMDEYDSLHWRRSLEEREALLRDISSRQALGLPLEEPGRYKPGSFFGKDQYDPTSALYQYDYWGEPKNSEISKQERMKDAHNKSIVGKGNVWYDMSYDDAIKQTIERRKAESNVVTQKEEETESKEEEEDDDDEYEFDDFDYSILSDESSIGYSEQQPLVNGTQVFTD

Entity 8 polymer

描述 description	PAP4, FSD3
分子量 formula_weight	30418.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	I

MASCVVTTSSFCTLSDSNIRLKSSKLVHLSNQQRRRFPLGSRGGLKVEAYYGLKTPPYPLDALEPYMSRRTLEVHWGKHHRGYIDNLNKQLGKDDKLYGYTMEELIKATYNNGNPLPEFNNAAQVYNHDFFWESMQPGGGDMPQKGVLEQIEKDFGSFTNFREKFTNAALTQFGSGWVWLVLKREERRLEVVKTSNAINPLVWDDIPIISLDVWEHSYYLDYKNERGKYINTFLNHLVSWNAAMSRMARAEAFVNLGEPNIPIA

Entity 9 polymer

描述 description	PAP5, pTAC12, HEMERA
分子量 formula_weight	60884.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J

MASISTTSWLYRDKLCTESGKLGTCILQRPVKCGFPVKRLYVGITSKDVLMRDCIKCKKDDDDDDASEGSSKKDGQGYEYVSVERAPYYSYMDSTSGKMEPASGARASIPGEDYWPEGTSSRVRAARAPQPAGESSSFPSYGKNPGSRRKKNRKATEGNAAVETYDEVSDSEDSSEEEESDSSNGFVVYNNEVEGEDEEETGFELDKKLGRPHPFIDPTKKKQIETTLTSDESWWNWRKPEKEQWSRWQRRRPDVETVFLKAMAETGQVKLYGKEPTLTETSLYRARRHLFKEERLQAERERLAKEGPMAFYSEWVKAWKRDTSREAVQKHFEETGEDENTQLIEMFSHQTDREYRIMMGTDVRIKRDPLAMRMKEDQIKQIWGGDPVYPTINYIQAPDAVMDFRGPDFHEPTPNMLSYLKENCKVISREMHETLLAKEKTEQVEVPDIDDAMAQAVDIGENDDEEEDTEEAEKDEKVARNWSVLKSTPELRNSKPKPKKEGRMSLDEAVDDSENLTDFLMDFDEETDP

Entity 10 polymer

描述 description	PAP6, FLN1
分子量 formula_weight	52435.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K

MASLHLFPHLHHFDSIIHRREIAAHSRRQFLSPKASINGGVVTNGASAAETAKPSRKGRNKKKQTEETNPDSDPALVDYDDGIDFPYDDPPLVCCFGAVQKEFVPVVRVHDNPMHPDIYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMGKVGGDDYGEELVLMMNKERVQTRGVKFDEGASTACTRVKIKFEDGKMKAETVKEPPEDSLLASELNLAVLKEARIFHFNSEVLTSPTMESTLFKAIQWSKKFGGLIFFDLNLPLPLWRSRNETRKLIKKSWDEANIIEVSQQELEFLLDEEYYERRRNYTPQYFAEDFEQTKNRRDYYHYTPEEIKPLWHDDLKLLVVTDGTLRLHYYTPKFDGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTCPEMFEDQDVLERQLRFAVAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW

Entity 11 polymer

描述 description	PAP7, pTAC14
分子量 formula_weight	55675.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

MASPVSLHYLINTFISKPQGFCNGTVSSPRPRSSFVRERQNSVKPIKVASLETQPFPLFQSPASEESSSSELESADPDFYKIGYVRRVRAYGVEFKEGPDGFGIYASKDIEPRRRARVIMEIPHELMITIRQKHPWMFFPDIVPIGHPIFDIINSTDPERDWDLRLACLLLFSFDREDHFWRLYGDFLPAADECSSLLLATEEDLAELQDPQLVSTIRQQQKRVLEFWEKNWHSGVPLKIKRLAEDAERFIWAVSIAQTRCISMKTRIGALVQDLNMMIPYADMLNHSFEPNCFLHWRPKDRILEVMSNAGQAIKKGEEMTINYMPGQKNNMLMERYGFSTPVNPWDAIPFSGDSRIHLNSFLSVFNIFGLPEEYYHDSELSGDDSFVDGAVIAAARTLPTWSDIDLPPIPSAERKAVKELQDECRKMLAEYPTTSEQDQKLLDSLSEARTTFATAVKYRMHRKMFIGKIIKALDIYQERLLF

Entity 12 polymer

描述 description	PAP8, pTAC6
分子量 formula_weight	38039.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

MASSAASPSLSLLSLTPKPPPSPSTASATSHRLFPSFRTNGCFAPLTLKPRRGRSIIVKVDDGDADGGGQDEYDMDDEEVEEVDNKKDYDVEYDPLAAAMAAASGGGGDGDIAFVQSKSFISTQGWDSEMVVDYRINEDEFHKISLLDCDFFIRKPPDPDNDVYDFREMYVTPPDTDIYSVPRVLAPMPQKYIRCAMSDYGCYDVTEPPIDAPRDPLYKSEREISKVFLTKHYRNRRLNDPEFVLDFEEIYVIDSKTKSITRARVLVTVPGGRKRDRKDDLLVIRDNGNSFKIIHVGERDDPTTVIEREEWTKTREDMEKHLRKLRDFSVSNWF

Entity 13 polymer

描述 description	PAP9, FSD2
分子量 formula_weight	34008.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

MMTSTCSFLSSCSLLPSQEPNQRMQWKRHEKRQFSRKVAVSGVIRAGFELKPPPYPLDALEPHMSRETLDYHWGKHHKTYVENLNKQIVGTDLDGLSLEEVVLLSYNRGNMLPAFNNAAQAWNHEFFWESIQPGGGGKPSGDLLRLIERDFGSFSDFVERFKAAAASNFGSGWTWLAYKANRLDVANAVNPLPKEEDKKLVIVKTPNAVNPLVWDYSPLLTIDTWEHAYYLDFENRRIEYINTFMEKLVSWETVSTRLESAMARAAQREQEGSDTEAEENPDDEEPEVYLDDASEVD

Entity 14 polymer

描述 description	PAP10, TRXz
分子量 formula_weight	20954.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O,P

MALVQSRALPRLNVSLSPILSTLHAPPSSLFLRREIRPVVTSPFSSSTTGNLPFSPLTHPRKILCPPPRGKFVREDYLVRKLSAQELQDLVKGERKVPLIVDFYATWCGPCILMAQELEMLAVEYESNAMIVKVDTDDEYEFARDMQVRGLPTLFFISPDPSKDAIRTEGLIPLQMMRDIIDNDM

Entity 15 polymer

描述 description	PAP11, MurE-like
分子量 formula_weight	85121.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q

MAFTFFSPHPVFLPLGRTTSSFSYKPVYSPFPRNSRNLQLAAGPARRNSYPNPADDDPPEAPEDSMHGVSKFQQIQRQAARARKLEEEDFEKNRNTYLSAIADVEDAPETGRDDVESGGDLFSDIDRAISMKRSEFVKKGLLQPNPPKTTSSKKLDEEEDDVTDVVDELDEEEVVDLDEIDKLTGLTEVSDEEDWVDEEGNPRINKKKETGSDHHFEFDLDDFGGSKARIVEPKFRLSLAELLDESKVVPISVYGDLDVEITGIQHDSRGVSSGDLFVCCENEGDSVLSEADKRGAVAVVASKEIDIEDTLGCRALVIVEDTEAVLAALASSFYRHPSKNMAVIGVTGTNGKTTTTYLIKSLYEAMGVRTGMFSTVSCYVHGDNKMDSPTTTSPDAVLVQSMMAKMLHNGTEALVMEASPQELASGKCDEVDFDIAVFTNLTREDSDFRGTDEEYRDAEAKLFARMVDPERHRKVVNIDDPNAAFFVQQGNPDVPVVTFAMENTKADVHPLKFELSLFETQVLVNTPQGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGVEEVDAVPGRCELIDEEQAFGVIVDHANTPDGLSRLLDSVRELKPRRIITVIGCAGENERGKRPVMTKIATEKSDVTMLTSDNPGNEDPLDILDDMLSGIGWTMQEYLKHGEHDYYPPLSNGHRLFLHDVRRVAVRCAVAMGEEGDMVVVAGKGHESYQLEGDKKEFYDDREECREALQYVDELHQAGIDTSEFPWRLPESH

Entity 16 polymer

描述 description	PAP12, pTAC7
分子量 formula_weight	18835.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R

MFCSSFTSSISRIGDARSGNSRASSFTFQTQVSCGIQRDDNGRRIWRRRTLTKKDDMLRYKLQRVPFVEEQVRKIKEVGKVMTMDIERLLLSEDNRFEFVNSVAAEATEYVEKNRDEYGGTKKAIFHVLSNRVNDLGFDRPEAYAESDPYKPGPGYLKEYYT

Entity 17 polymer

描述 description	FLN2
分子量 formula_weight	68527.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S

MASLSFTQFLPFPRCSVDVPCLQPHGFVKFRGERWKGKHSFLMVAGRRKLSESAPLDEDDGGNGAVGGKKPTKVPKKSGARTAKKKVVAKDEPLEESSQLLVDSDNVSDNESDTKEPVRRTRKKAAASSDVNEGKTEKKVRRKRTVKKDKEVEDGLVTYDEASDVEEALTVEATDADSEGEEIDLSKHESEDISHTYGWPPLVCCFGSAQHAFVPSGRPANRLLDYERQERMKDAVWAPEKYIRAPGGCAGGVAIALASLGGKAAFMGKLGDDDFGQAMLYYLNVCQVQTRSVKIDSKRVTACSTMKISKRGRLKSTCVKPCAEDSLSKSEINVDVLKEAKMFYFTTHSLLDKKMMSTTLQAIKISKQLGNVIFYDLNLPLPLWQSLEETKSLIQEVWDLADVIEVTKQELEFLCGIEPTEEFDTKNNDSSKFVHYEPETVEPLWHENLKILFVTNGTSKIHYYTKEHNGAVLGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNDKGYLERTARYAIECGVVDQWLLAQTRGYPPKDDMEEEEDDDEEEEMESDPNGIRSITEREYRTSKPYDEPDGPYVMKPVEEREYRKLELVGSMGEDDDSS

Entity 18 polymer

描述 description	pTAC18
分子量 formula_weight	16430.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T

MASLIMTTPFSGSLTQCKKKTNNLSVNRAFFKVTCSMQTPLEELYNVKVERKVSQRRLDELGVSRWSVWKTGKCKLPWDWQVDQLVYIEEGEVRVVPEGSKRFMQFLAGDLVRYPKWLEADLFFNAPYSERYCFKAYADD

Entity 19 polymer

描述 description	PRIN2, PLASTID REDOX INSENSITIVE 2
分子量 formula_weight	20915.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	U

MQMASLDAALFSSRLQFHSSSSSSSSIRCALPTIFNLSSPAGSSCVLKSTQFLKRRLRAKSTNFSLSSLPRRGFVCRAAEYKFPDPIPEFAEAETKKFRDHMAKKLAKRDLFEDSVDEIVGVCTEIFETFLRNEYGGPGTLLVIPFIDMADTLNERELPGGPQAARAAIKWAQDHVDKDWKEWTGTD

Entity 20 non-polymer

描述 description	2,3-DIHYDROXY-1,4-DITHIOBUTANE
分子量 formula_weight	154.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 21 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 22 non-polymer

描述 description	FE (III) ION
分子量 formula_weight	55.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 23 non-polymer

描述 description	CALCIUM ION
分子量 formula_weight	40.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 24 non-polymer

描述 description	POTASSIUM ION
分子量 formula_weight	39.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 25 non-polymer

描述 description	S-ADENOSYL-L-HOMOCYSTEINE
分子量 formula_weight	384.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.000 Å
最低分辨率 resolution_low	— Å
R 因子 r_factor	—
R_work r_work	—
R_free r_free	—

分辨率 resolution	3.00 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	105796

fit	/app/data/saxs_computation/ep/9epc/9epc.fit
pddf	/app/data/pr_computation/ep/9epc/9epc.pr.out
profile	/app/data/saxs_computation/ep/9epc/9epc.dat

Cryo-EM structure of the Plastid-encoded RNA polymerase from Sinapis alba

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

冷冻电镜 EM 3D

5. 实体与聚合体信息 (25)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 non-polymer

Entity 21 non-polymer

Entity 22 non-polymer

Entity 23 non-polymer

Entity 24 non-polymer

Entity 25 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)