9evd

In situ structure of the peripheral stalk of the mitochondrial ATPsynthase in whole Polytomella cells

Method: ELECTRON MICROSCOPY D_max: 209.1 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9evd
沉积日期 deposition_date	2024-03-29
最后修订 last_revision	2024-09-18
结构标题 title	In situ structure of the peripheral stalk of the mitochondrial ATPsynthase in whole Polytomella cells
关键词 keywords	ATP synthesis, in situ, OXPHOS, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	54.80 Å
回转半径 Rg (电子) rg_electron	55.82 Å
零角强度 I(0) i0	497557000.00
分子量 molecular_weight	191840.0 kDa
排除体积 excluded_volume	243080 Å³
包络体积 envelope_volume	395890 Å³
水化壳体积 shell_volume	64328 Å³
包络直径 envelope_diameter	222.9 Å
壳层 Rg shell_rg	49.85 Å
包络 Rg envelope_rg	58.53 Å
形状 Rg shape_rg	55.80 Å
总 Rg total_rg	55.68 Å
总原子数 total_atoms	13561
残基数 n_residues	1747
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	209.1 Å
Rg (实空间) rg_real	55.61 Å
Rg 误差 (实空间) rg_real_error	3.43 Å
I(0) (实空间) i0_real	4.9750e+08
I(0) 误差 (实空间) i0_real_error	1.2430e+07
Rg (倒空间) rg_reciprocal	54.12 Å
I(0) (倒空间) i0_reciprocal	496500000.0000
解质量估计 total_estimate	0.7519
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	43.3 Å
偏度 Skewness skewness	0.711
峰度 Kurtosis kurtosis	-0.008
角度范围 angular_range	— – 0.1450 Å⁻¹
当前正则化参数 α current_alpha	0.0001
最高正则化参数 α highest_alpha	51740000.0000
实空间数据点数 n_real_points	30
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.464; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.582; Smooth: 0.796

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (9)

Entity 1 polymer

描述 description	ASA-10: Polytomella F-ATP synthase associated subunit 10
分子量 formula_weight	8731.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	0
原子数 atom_count	13561

MSYSAYFAKAGFQFPAGLSALVAGIVALNVCTGRPTKGTKEISNAEYNATPIGYLQSPDQHPTAFPKVPGMKDVHGSPHHHH

Entity 2 polymer

描述 description	ATP synthase associated protein ASA1
分子量 formula_weight	68679.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1

MMRAAQKAKQELPATVLTQTRSYLAPLRSDFTEEITAPKVASASNLVNEWNNKKQATENLMKLLQAYKDIGDAKSEPLLKNHNPRTFEDRDYPVPDFRTQNLKAGDVPKFFDTVISTRASAAIASKDKFWAGRKTEAEAASAKASAAFPRVAVPEWKKGKTVSIENLNTVTDKYAAALVPKRKLALPVLPEGVKKAVEDFAASVGQAKNASEVSELLAKSLAEKAVVTEGGKVVEGFSYVSKAVAAKVIATRRAEVHERLLKLWAKRLLVSPELAIVPLNEFDAQLASKFEGISPKYQELLSAVAQGNKTFAQRLNSSPAFSSFLLKREKAESEVPPSELELEAAQKAAELEDPEVALRTLLGPQMEALGASDLLLSEQIRVITEHRYTPDRLQYKEGMKLADKIAAQEAALKEELKVIYGDNVDVKHFQASPRTPVQQLFDSLKNAAANKERAAKEAAAAASPYLAYAVTKKQEVQADPSNIPFDEVLYPQLSEELLELELSDIREDEIALEKAEEEELWLLTLTQQFKHIQKHFGIDLPHSVVAHMDPLLIKKIDWETTNALEDFDITLDDMGAEDAKEQWGAENLSHHFLPLIRYRRDLARKNGDRYGPDLVNGN

Entity 3 polymer

描述 description	Mitochondrial F1F0 ATP synthase associated 32 kDa protein
分子量 formula_weight	34850.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3

MRQASRLALSIRQAGNVEAASAVPAMTRQFSAPGSHEHHETPLSKVMPTVVSIPRKVACLALGATKKVVCGLASSGPSQNLVSTFANKVIVEENLVNVAEIDVPFWSYWLSSAGFTSKDAFVKFAEAVKPKVAALSTSDITNLTVAFKRANYYDKDLFTGIEANVSANFTKFETEQLLQIVATFDAFNHSSVAFLDDVADSITYCNHYLAPVRAGADELATLLTYYAKNGHERADLLATVARGFSEVSLGKLSAAQRKDTVLSALKAFQTFGFYPESIEAVIGAALVSPAEYSAEELKEVEAVKVAAENALGGEFVLIQEGAHGH

Entity 4 polymer

描述 description	Mitochondrial F1F0 ATP synthase associated 14 kDa protein
分子量 formula_weight	14004.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	5

MKLLPESLQQEAATAAVVASWVLWHLDTQLLPTIMREHKLHACWAAAAKRYNEKLFKLNPSYDRVLSLPAVSKNQVLENVFHTAPKAPVEHLEKMVSANSKVYDALNLQSKRVLIWQVKPALF

Entity 5 polymer

描述 description	Mitochondrial ATP synthase subunit ASA6
分子量 formula_weight	15904.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	6

MMLRTLTRSSAVAGQAVRLFKTSAAAAEGNSVAGIIKSVNETSGANLLSSLKTIKAQAAPIYPAAASSTGYSTQAKIALFGALSWILYRADGQSKAHEWIVDLNLNVLQAAWLISFSSLIPFRAVYFAFRGMAPATASTLNGLKTFSSISL

Entity 6 polymer

描述 description	Mitochondrial ATP synthase associated protein ASA7
分子量 formula_weight	20553.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	7

MSSVRAGVEAGRRDLTTFTFSGLQDAPVAALSGSIKLNVAAKAGKAEVTVAAGAAKAATQVSAAALRKLSGSKISLAEVARISVLHSSIQNYLLSLSNERYQLLSQWPDFTTMYGKDFYYRAHPEDLKKFYDAADEYYKLYETVTEFDSLSALASQVVPNYAARRRSTVHPAIGSTVADGAFTNFLLSKQ

Entity 7 polymer

描述 description	Mitochondrial ATP synthase subunit ASA8
分子量 formula_weight	9883.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	8

MVLGEVYLKDILRTPPTGAIPANVPHPFQTSFYTYATKKLIPRHWYLLGGFTFTITLYGILDGLRDSGKKKAYDEAIHAGKTPYTAGGH

Entity 8 polymer

描述 description	ASA-9: Polytomella F-ATP synthase associated subunit 9
分子量 formula_weight	11001.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	9

MAVTSFLGKAFEKYFYDFSAYEQFGLNRFLSSKGQYVALRHVGFVMVGVNVLLAANFPFNPPFPTIGMCPAGWEGTWVCQADKAKALEMYKEWKKSN

Entity 9 polymer

描述 description	Mitochondrial ATP synthase subunit 6
分子量 formula_weight	34802.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

MSVLSSVSMGSRIGSSLLGRSSAYLAQCGFSTRSNLNGSIDTSSSVFQALSSDNENKPAASPLNVKLPGMSCSSILLPKTSRIAVPFGNQTMAMSSVRDVKTGSLPTNFLTGVYRFWRSQNPAEKPHDPVNDRLLPAVVDASDKRASIGTWATTFFCTIISCNLLGLMPFNEAPTSGLGFATGLGVSVWATATILGLSKTGFKFPGHFIPGGTPWPMAFIFVPLETISYTFRAVSLGVRLWVNMLAGHTLLHILTGMALALPFSLGFFSMVPATFGVCCLLSALVGLEYLVAVLQSGVFSILSTVYVGEFNHDKFIGPAAKIVKKIH

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7. 引用文献 (3)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	5.60 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	238622

fit	/app/data/saxs_computation/ev/9evd/9evd.fit
pddf	/app/data/pr_computation/ev/9evd/9evd.pr.out
profile	/app/data/saxs_computation/ev/9evd/9evd.dat

In situ structure of the peripheral stalk of the mitochondrial ATPsynthase in whole Polytomella cells

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (9)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

7. 引用文献 (3)

8. 文件与曲线 (10)

Files (7)

Curves (3)