9glc

NONO/SFPQ filament: local refinement central units (strand 1)

Method: ELECTRON MICROSCOPY D_max: 206.3 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9glc
沉积日期 deposition_date	2024-08-27
结构标题 title	NONO/SFPQ filament: local refinement central units (strand 1)
关键词 keywords	filament, RNA binding, DNA binding, gene regulation, NUCLEAR PROTEIN; NUCLEAR PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	63.01 Å
回转半径 Rg (电子) rg_electron	64.17 Å
零角强度 I(0) i0	690291000.00
分子量 molecular_weight	205960.0 kDa
排除体积 excluded_volume	252630 Å³
包络体积 envelope_volume	445670 Å³
水化壳体积 shell_volume	62043 Å³
包络直径 envelope_diameter	247.7 Å
壳层 Rg shell_rg	56.03 Å
包络 Rg envelope_rg	63.73 Å
形状 Rg shape_rg	64.18 Å
总 Rg total_rg	63.91 Å
总原子数 total_atoms	14423
残基数 n_residues	1728
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	206.3 Å
Rg (实空间) rg_real	63.79 Å
Rg 误差 (实空间) rg_real_error	1.74 Å
I(0) (实空间) i0_real	6.8940e+08
I(0) 误差 (实空间) i0_real_error	1.4370e+07
Rg (倒空间) rg_reciprocal	62.05 Å
I(0) (倒空间) i0_reciprocal	687900000.0000
解质量估计 total_estimate	0.7968
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	49.5 Å
偏度 Skewness skewness	0.544
峰度 Kurtosis kurtosis	-0.460
角度范围 angular_range	— – 0.1250 Å⁻¹
当前正则化参数 α current_alpha	0.0028
最高正则化参数 α highest_alpha	40660000.0000
实空间数据点数 n_real_points	26
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.778; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.869; Smooth: 0.154

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)
分子量 formula_weight	75880.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	BC,BE,AC,AE,CC,CE
原子数 atom_count	14423

MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGGPKPPIPPPPPHQQQPQQPPPQQPPPQQPPPHQQPPPHQPPHQQPPPPPQDSSKPVVPQGPGSAPGVSPAPPPAGSAPPANPPTTGAPPGPGPTPTPPPAVTSATPGPPPPSTPSSGVSTTPPQSGGPPPPPAGGAGPGPKQGPGPGPGGPKGGKMPGGPKPGGGPGMGAPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPAARTEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHSQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGTMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF

Entity 2 polymer

描述 description	Non-POU domain containing octamer binding
分子量 formula_weight	54519.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	BD,BF,AD,CF

MQSNKTFNLEKQNHTPRKHHQHHHQQHHQQQQQQQQQPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPTGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRPAPGADFAPNKRRRY

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.30 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	2974535

fit	/app/data/saxs_computation/gl/9glc/9glc.fit
pddf	/app/data/pr_computation/gl/9glc/9glc.pr.out
profile	/app/data/saxs_computation/gl/9glc/9glc.dat

NONO/SFPQ filament: local refinement central units (strand 1)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)