9h5i

Haemophilus influenzae serotype b capsule polymerase bcs3 in complex with acceptor polymer

Method: ELECTRON MICROSCOPY D_max: 180.9 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9h5i
沉积日期 deposition_date	2024-10-22
结构标题 title	Haemophilus influenzae serotype b capsule polymerase bcs3 in complex with acceptor polymer
关键词 keywords	Capsule polymerase, Haemophilus influenzae serotype b, Pathogens, BIOSYNTHETIC PROTEIN; BIOSYNTHETIC PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	50.90 Å
回转半径 Rg (电子) rg_electron	50.92 Å
零角强度 I(0) i0	884398000.00
分子量 molecular_weight	249140.0 kDa
排除体积 excluded_volume	312360 Å³
包络体积 envelope_volume	478070 Å³
水化壳体积 shell_volume	81201 Å³
包络直径 envelope_diameter	179.0 Å
壳层 Rg shell_rg	52.18 Å
包络 Rg envelope_rg	49.60 Å
形状 Rg shape_rg	50.94 Å
总 Rg total_rg	50.92 Å
总原子数 total_atoms	34123
残基数 n_residues	2226
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	180.9 Å
Rg (实空间) rg_real	51.08 Å
Rg 误差 (实空间) rg_real_error	1.96 Å
I(0) (实空间) i0_real	8.8440e+08
I(0) 误差 (实空间) i0_real_error	1.7610e+07
Rg (倒空间) rg_reciprocal	50.75 Å
I(0) (倒空间) i0_reciprocal	884000000.0000
解质量估计 total_estimate	0.8462
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	58.4 Å
偏度 Skewness skewness	0.474
峰度 Kurtosis kurtosis	-0.249
角度范围 angular_range	— – 0.1550 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	67710000.0000
实空间数据点数 n_real_points	32
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.761; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.925; Smooth: 0.790

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (4)

Entity 1 polymer

描述 description	Bcs3
分子量 formula_weight	137381.2 kDa
来源方法 src_method	man
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	34195

VDKTWLFGSYAWQGNPKALFLYMLVNCKETHECWWVADNEESMKSIKKSTGLKNITFTDSEKAKELFPHADVYVTENFRESYPVYMNENIKVFNTWHGVGLKHIELALGMNSVLAESIVRKYVRNYDIYKNNVLFLTTSQAMEDHFLEDMAISKELIIRGKYPRNAVYGPNGIHTYDINTLLPKNKSQYSQTILFCPTYRIGAIQGVLNSLLPDFAKLEEVCRHKNQLFIVKVHPFMKKDNYFAEMSEKYKDSEYILFWNDDYDIYEAFNSIDLAIIDYSSIFYDLLDAGVEKFIRYVPDLDEYQNDLELIGDYADLTEGRIVKSFQQLLNCLDNANIKIISTKRKQYLMDYFFGFKKENKSMESLIADVDNCQLQPKSLKELHTFDIFDTLIRRSTLRPFSIFDYVRDKAKASGIKFPLALTENWINVRNRAEHDVRDIMRKTTFERQSDKIEITLDDIYTRLQKNLLLTDEQTDFLKQAEIEAEIAHVEPIQKRINYLFSLKAKGHDVAMASDMYLPEDVIYKMLDRADTRLREIPLYLSSTIGYQKSTGKLYQHIFFDLDYQYSRWTHYGDNKHADGSVPRRLGIQTAVHDIDDFIPFENAMVNAMDNYNRYPAYQLATKMHRYRTQLVQENGFGNTLFETKYYNYAYVGASFVPYINWAIKDAIKRGYETIYFISRDGHFLKQIADKIIEIRGYNVKTKYIYGSRKAWRLPSFITKVDDETFWQFGNFVGMDSFEDLVKASYLSESELLSLFPEFESLRHAKHLRGEIAENIRKIFKNSPAYHEKVLAIAAEKRKMVRQYIQQEINPKEKFAFVEFWGRGYTQDTFGRLLNDAFGKEVKNPFYYVRSFTDDMGTSVRHNFILAPQNFSFFEPIFAQTPYDSIPDYYEEKGRIEPIINHRDRSVSDLISEGLLKFTEDYLALNTQDEDYFDAALSQFNYQYQLNTPNDQFICNVFSELKDNISSFGVEKPYAPALTLKQLESITSKQELDKLTQSIPISLSKSDVKVIDYYNKIQKNYNLPAYNSTPMRKAYAVNPLEQYVWSTQVPFRVLSLKQNSFYLDVSFAETTKRKDIFLKELNEIDVIAVDWLKGGVPRLLTEHGYITAHKDWVKKSFNDDKTNNIEEPKVKNKEKSKVLEVNTTVTNNNKQAIGKLDNNIDKSLEHHHHHH

来源 Source

来源生物 organism	Haemophilus influenzae
NCBI 分类编号 ncbi_taxonomy_id	727
宿主生物 host_organism	Escherichia coli

Entity 2 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 3 non-polymer

描述 description	beta-D-ribosyl-(1->1)-D-ribitol-5-phosphate
分子量 formula_weight	364.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	12

Entity 4 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	72

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.65 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	388941

fit	/app/data/saxs_computation/h5/9h5i/9h5i.fit
pddf	/app/data/pr_computation/h5/9h5i/9h5i.pr.out
profile	/app/data/saxs_computation/h5/9h5i/9h5i.dat